Abstract:
:Cell death induced by various radiation doses in Molt-4 and L5178Y cells was studied. Molt-4 cells have a low threshold for induction of interphase death while L5178Y cells have a high threshold. Interphase death in Molt-4 cells at doses > 100 Gy and in L5178Y cells at doses > 300 Gy showed typical necrosis. In contrast, cell death in Molt-4 cells after 2-30 Gy had features of both apoptosis and necrosis; the appearance of orcein-stained cells in the light microscope was suggestive of apoptosis, showing chromatin margination and apoptotic bodies. The concomitant appearance of non-viable cells as estimated by either orcein staining or eosin dye staining correlated well with this. Early cell swelling and a diffuse smear pattern in DNA gel electrophoresis were however suggestive of necrosis. Detailed electron microscopic observations revealed that events leading to the interphase death of Molt-4 cells after 2-30 Gy involved complicated pathways which included both apoptotic changes in the nucleus and necrotic changes in the cytoplasm. TPA not only had a protective effect on the interphase death of Molt-4 cells, but also converted the mode of cell death from interphase death to mitotic death after low doses of radiation. Cycloheximide and H-7, known to inhibit apoptosis in irradiated thymocytes, had no protective effect on the interphase death of Molt-4 cells, suggesting that the events leading to interphase death in Molt-4 cells might be different from those in thymocytes. BrdUrd-labelling had a sensitizing effect on the radiation response, but no effect on interphase death in Molt-4 cells thus suggesting that DNA damage may not be responsible for interphase death.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Akagi Y,Ito K,Sawada Sdoi
10.1080/09553009314551101subject
Has Abstractpub_date
1993-07-01 00:00:00pages
47-56issue
1eissn
0955-3002issn
1362-3095pii
UKKYG08KMVX2CX13journal_volume
64pub_type
杂志文章abstract:PURPOSE:5-Formyluracil (5-foU) is a major derivative of thymine produced in DNA by ionizing radiation and various chemical oxidants. It has been previously shown that 5-foU in template DNA directs misincorporation of nucleotides by DNA polymerases during in vitro DNA synthesis. The present experiments were designed to ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530001453113
更新日期:2001-01-01 00:00:00
abstract:PURPOSE:Radiotherapy is an important treatment option for brain tumors, but the unavoidable irradiation of normal brain tissue can lead to delayed cognitive impairment. The mechanisms involved are still not well explained and, therefore, new tools to investigate the processes leading to the delayed symptoms of brain ir...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1787542
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:Cartilage degradation usually results as a consequence of inflammatory processes in the joints. To study this phenomenon experimentally, adjuvant-induced arthritis (AIA) was used as a model of chronic inflammation under the influence of irradiation. The potential chondroprotective effect of 13% aqueous extract ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.530337
更新日期:2011-03-01 00:00:00
abstract::Solid human tumours contain areas with low oxygen tension (pO2). For bioreductive drugs it is important to define the cytotoxic effect according to drug concentration and to clinically relevant pO2. In this study, the pO2 dependence of the survival of three human cell lines (HRT 18, Na11 +, and MEWO), exposed to tirap...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514550261
更新日期:1995-02-01 00:00:00
abstract::BW12C, which was developed as an agent for the treatment of sickle cell anaemia, increases the binding of oxygen to haemoglobin and hence reduces the availability of oxygen to tissues. Due to these changes in oxygen availability BW12C could act as a protector against radiation-induced injury to normal tissues. In this...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550921
更新日期:1991-04-01 00:00:00
abstract:PURPOSE:A key enzyme of polyamine catabolism, spermidine/spermine N(1)-acetyltransferase (SSAT), is responsive to antiproliferative agents. The role of SSAT in cellular responses to X-ray irradiation was examined. MATERIALS AND METHODS:Exponentially growing HeLa S3 cells were irradiated by X-rays, and mRNA levels for ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000410001695886
更新日期:2004-05-01 00:00:00
abstract:PURPOSE:In order to understand the molecular mechanism of nucleobase damage caused by ultrasoft X-ray irradiation, guanine radicals have been studied using an X-band EPR (electron paramagnetic resonance) spectrometer installed in a synchrotron soft X-ray beamline. MATERIALS AND METHODS:Guanine pellets were irradiated ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000400017689
更新日期:2004-11-01 00:00:00
abstract:PURPOSE:Human embryonic stem cells (hESC) hold a great potential for regenerative medicine because, in principle, they can differentiate into any cell type found in the human body. In addition, studying the effect of ionizing radiation (IR) on hESC may provide valuable information about the response of human cells to I...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.700435
更新日期:2012-12-01 00:00:00
abstract::In this report, in situ hybridization with whole chromosome painting probes was used to paint radiation-induced micronuclei (MN) in three lymphoblastoid cells lines to investigate the frequency of radiation-induced MN. The results obtained for four different chromosomes showed that there was a significant deviation of...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096144860
更新日期:1996-10-01 00:00:00
abstract:PURPOSE:To follow plasma Flt3-ligand (FL) concentrations in irradiated animals in order to evaluate it as an indicator of bone marrow damage for the management of accidental radiation-induced aplasia. MATERIALS AND METHODS:Non-human primates were irradiated at doses ranging from 2 to 8 Gy, using whole- or partial-body...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110043711
更新日期:2001-06-01 00:00:00
abstract:PURPOSE:To investigate the hypothesis that the developmental phase at which an individual encounters radiation damage affects its long-term sensitivity to a subsequent tumourigenic insult. MATERIALS AND METHODS:Either the pregnant C57B16 mouse was exposed to 137Cs gamma-rays at 4 or 15 days post-conception (embryonic ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000139032
更新日期:2000-01-01 00:00:00
abstract:PURPOSE:Adverse effects on human health caused by electromagnetic fields (EMF) associated with the use of mobile phones, particularly among young people, are increasing all the time. The potential deleterious effects of EMF exposure resulting from mobile phones being used in close proximity to the brain require particu...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2016.1206223
更新日期:2016-10-01 00:00:00
abstract:PURPOSE:Solar energy has a number of short- and long-term detrimental effects on skin that can result in several skin disorders. The aim of this review is to summarise current knowledge on endogenous systems within the skin for protection from solar radiation and present research findings to date, on the exogenous opti...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.3109/09553002.2010.501842
更新日期:2010-12-01 00:00:00
abstract:PURPOSE:Following ionizing radiation exposure, intestinal crypt regeneration is possible but it is still not known if regenerating crypts give rise to differentiated functional epithelial cells on villi. The aim of this study was to demonstrate that irradiated progeny of enterocytic precursor cells are capable of proli...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000500091923
更新日期:2005-03-01 00:00:00
abstract:PURPOSE:The linear nonthreshold (LNT) model has been used in radiation protection for over 40 years and has been hotly debated. It relies heavily on human epidemiology, with support from radiobiology. The scientific underpinnings include NCRP Report No. 136 ('Evaluation of the Linear-Nonthreshold Dose-Response Model fo...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2017.1328750
更新日期:2017-10-01 00:00:00
abstract:PURPOSE:To describe the design and dosimetric characterization of a simple and economical small animal irradiator. MATERIALS AND METHODS:A high dose rate (HDR) (192)Ir brachytherapy source from a commercially available afterloader was used with a 1.3 cm thick tungsten collimator to provide sharp beam penumbra suitable...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.922719
更新日期:2014-10-01 00:00:00
abstract::The effects of UV radiation on humans and animals are receiving increasing attention and much interest has recently been focused on the environmental effects of UV A and UV B. This study compares the in vitro effects of UV A and UV B on the clonogenic survival of two human skin keratinocyte cell lines, HaCaT which are...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143590
更新日期:1997-07-01 00:00:00
abstract:PURPOSE:To investigate the influence of chromatin organization and scavenging capacity in relation to irradiation temperature on the induction of double-strand breaks (DSB) in structures derived from human diploid fibroblasts. MATERIALS AND METHODS:Agarose plugs with different chromatin structures (intact cells+/-wort...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000310001610204
更新日期:2003-10-01 00:00:00
abstract::Samples of human rib were collected at autopsy and 20 were selected for marrow fat cell measurement, representing an age range of 16-96 years. The mean diameter of fat cells in red bone marrow of human rib was found to increase from around 48 microns at ages 16-29 years to around 65 microns at ages 82-96 years. There ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514551681
更新日期:1995-12-01 00:00:00
abstract:PURPOSE:To investigate the mechanism of methylene blue-induced radiosensitization in Escherichia coli cells. MATERIALS AND METHODS:Bacteriophage lambda15 was irradiated with X-rays in the presence or absence of methylene blue (MB) and infected into bacteria with different repair capabilities preincubated with or witho...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050134528
更新日期:2000-09-01 00:00:00
abstract::The dose-rate effect has been the topic of extensive radiobiological studies and has important implications in radiation therapy and in the field of radiation protection. Three examples will be discussed: two in radiation therapy and one in protection. First, continuous low dose-rate interstitial brachytherapy may be ...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009114550531
更新日期:1991-03-01 00:00:00
abstract::This study simulates the exposure of V79 Chinese hamster fibroblasts to low-energy protons, deuterons and alpha-particles in the LET range 10-200 keV/microm. The starting assumption is that the induction of clustered lesions in DNA is a fundamental step for cell inactivation. A non-homogeneous cell population was simu...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143004
更新日期:1997-11-01 00:00:00
abstract:PURPOSE:Since the exact development of posterior subcapsular cataracts (PSCs) is poorly understood, we review various risk factors and propose a two-stage etiology for PSCs. METHODS:The biological mechanisms associated with age-related cataracts (primarily nuclear cataracts, cortical cataracts and PSCs) were reviewed ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1812759
更新日期:2020-11-01 00:00:00
abstract:PURPOSE:Melanoma is mainly treated by surgery and rarely with radiation because of the high radioresistance of this tumor. Nevertheless, radiotherapy is the preferred treatment modality for unresectable lesions and avoiding cosmetic disfigurement caused by surgical excision. This study investigated the therapeutic adva...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2017.1276309
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:The long-term effect of low and moderate doses of ionizing radiation on the lens is still a matter of debate and needs to be evaluated in more detail. MATERIAL AND METHODS:We conducted a detailed histological analysis of eyes from B6C3F1 mice cohorts after acute gamma irradiation (60Co source; 0.063 Gy/min) at...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2021.1876951
更新日期:2021-01-19 00:00:00
abstract::The kinetic scheme of the reaction of desferrioxamine (DFO) with O2-. was studied using pulse and gamma-radiolysis. The rate constant k(O2-. + DFO) is equal to 1.3 +/- 0.1 x 10(6) dm3 mol-1s-1 at pH 7.4. Studying the competition between DFO and ferricytochrome-c for O2-. generated by gamma-radiolysis, we observed that...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914552381
更新日期:1989-12-01 00:00:00
abstract:PURPOSE:Angiotensin-converting enzyme (ACE) inhibitors are effective in the prophylaxis of radiation-induced renal and lung injury. Studies were designed to determine whether blocking the angiotensin II (AII) receptor, rather than blocking AII synthesis with ACE inhibitors, would also be effective. MATERIALS AND METHO...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098142257
更新日期:1998-04-01 00:00:00
abstract::It follows from the present work that the vascularization of the normal tissue present in the treatment volume limits the temperatures achieved during heat treatment of invasive ductal breast carcinoma. The temperatures can often be increased by giving fractionated heat treatment because heat treatment may reduce the ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114551821
更新日期:1991-07-01 00:00:00
abstract:PURPOSE:A model that identifies radiation-induced genetic instability as the earliest cellular event in the multi-step sequence leading to radiation-induced cancer was previously proposed. In this paper ongoing experiments are discussed which are designed to test this model and its predictions in mouse mammary epitheli...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/095530098141023
更新日期:1998-12-01 00:00:00
abstract::The aim was to identify the decorporation and anti-oxidation efficacy of prompt and delayed consecutive administration of the catecholicpolyaminopolycarboxylate ligands 9501 and 7601 for radiothorium in vivo. The chelating agents 9501 or 7601 were administered intramuscularly to ICR mice 3 min or 3 days after intraper...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000500141124
更新日期:2005-04-01 00:00:00