Abstract:
PURPOSE:To investigate the mechanism of methylene blue-induced radiosensitization in Escherichia coli cells. MATERIALS AND METHODS:Bacteriophage lambda15 was irradiated with X-rays in the presence or absence of methylene blue (MB) and infected into bacteria with different repair capabilities preincubated with or without MB. The survival of the bacteriophage in each bacterial strain was used to quantify MB-induced radiosensitization. DNA repair in bacteria irradiated with X-rays and incubated with or without MB was examined by alkaline and neutral sucrose gradients. An in vitro repair system of pBR322 plasmid DNA irradiated with X-rays was designed to determine the repair enzyme targeted by MB. CONCLUSIONS:MB impairs the repair activity of the polymerase 1 enzyme in E. coli cells, sensitizing these bacteria to the lethal effects of ionizing radiation. Since MB accumulates preferentially in some malignant tumours, it will be of interest to investigate its effects on the repair of irradiated human cells.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Menezes S,Costa JAdoi
10.1080/09553000050134528subject
Has Abstractpub_date
2000-09-01 00:00:00pages
1289-94issue
9eissn
0955-3002issn
1362-3095journal_volume
76pub_type
杂志文章abstract:PURPOSE:Ionizing radiation has been recognized to increase the risk of cardiovascular diseases (CVD). However, there is no consensus concerning the dose-risk relationship for low radiation doses and a mechanistic understanding of low dose effects is needed. MATERIAL AND METHODS:Previously, human umbilical vein endothe...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.905724
更新日期:2014-07-01 00:00:00
abstract:PURPOSE:To investigate the influence of chromatin organization and scavenging capacity in relation to irradiation temperature on the induction of double-strand breaks (DSB) in structures derived from human diploid fibroblasts. MATERIALS AND METHODS:Agarose plugs with different chromatin structures (intact cells+/-wort...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000310001610204
更新日期:2003-10-01 00:00:00
abstract:BACKGROUND:Element-specific biokinetic models are used to reconstruct doses to systemic tissues from internal emitters. Typically, a systemic model for a radionuclide explicitly depicts only its dominant repositories. Remaining tissues and fluids are aggregated into a pool called Other tissue in which the radionuclide ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2018.1554923
更新日期:2018-12-04 00:00:00
abstract:PURPOSE:DNA damage is one of the main consequences of exposure to ionizing irradiation (IR). Recent studies indicate that IR can modulate the expression of immune system-related genes. However, the effects of IR on the expression of genes and pathways of the B7-CD28 superfamily remain poorly defined. The aim of this st...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2021.1857454
更新日期:2020-12-17 00:00:00
abstract:PURPOSE:To evaluate and optimize the radioprotective ability of the most potent fraction of an aqueous extract of Coronopus didymus in whole body gamma-irradiated Swiss albino mice and to evaluate the antioxidant status and lipid peroxidation of the livers of the surviving mice. To correlate the free radical scavenging...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000600876686
更新日期:2006-08-01 00:00:00
abstract::BW12C, which was developed as an agent for the treatment of sickle cell anaemia, increases the binding of oxygen to haemoglobin and hence reduces the availability of oxygen to tissues. Due to these changes in oxygen availability BW12C could act as a protector against radiation-induced injury to normal tissues. In this...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550921
更新日期:1991-04-01 00:00:00
abstract:PURPOSE:To follow plasma Flt3-ligand (FL) concentrations in irradiated animals in order to evaluate it as an indicator of bone marrow damage for the management of accidental radiation-induced aplasia. MATERIALS AND METHODS:Non-human primates were irradiated at doses ranging from 2 to 8 Gy, using whole- or partial-body...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110043711
更新日期:2001-06-01 00:00:00
abstract::Objective: To quantify DNA damage in patients undergoing non-contrast and contrast-enhanced 18F-FDG PET/CT whole body positron emission tomography/computed tomography (WB PET/CT) investigations using comet assay technique and micronucleus assay, and to study the effect of other baseline parameters of patients on DNA d...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1577569
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:To investigate the potential total and relative free-radical transfer from histones to DNA in X-irradiated chromatin, by analysing the relative contributions of individual radicals from protein and from DNA. MATERIALS AND METHODS:Chromatin was isolated from calf thymus, freeze-dried and either used as such or ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050111541
更新日期:2000-08-01 00:00:00
abstract:PURPOSE:To resolve the difficulty in counting merged DNA damage foci in high-LET (linear energy transfer) ion-induced patterns. MATERIALS AND METHODS:The analysis of patterns of RIF (radiation-induced foci) produced by high-LET Fe and Ti ions were conducted by using a Monte Carlo model that combines the heavy ion trac...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553001003717175
更新日期:2010-06-01 00:00:00
abstract:PURPOSE:Hypoxic cells in tumours are resistant to 5-fluorouracil (5-FU). This in vivo study investigated the ability of hypoxia to regulate the gene expression of thymidylate synthase (TS), the target enzyme of 5-FU. MATERIALS AND METHODS:C3H mammary carcinomas, grown in the feet of female CDF1 mice, were used for all...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139944
更新日期:1999-07-01 00:00:00
abstract:PURPOSE:The nature of the transferrable factor which goes from irradiated objects to bystander objects remains undefined. Most agree that a chemical entity is the likely 'factor' although some authors have produced in vitro evidence for the involvement of a physical component or a very potent volatile capable of travel...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.698366
更新日期:2012-08-01 00:00:00
abstract::Swiss albino mice (7-8 weeks old) were irradiated with different doses (0-25 Gy) of gamma-radiation at a dose-rate of 0.05 Gy/s. The specific activities of glyoxalase I (GI) and glyoxalase II (GII) were determined in the spleen and liver immediately and on the 3rd and 6th day postirradiation. The results indicate that...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314550301
更新日期:1993-02-01 00:00:00
abstract::Cells use substrates such as glucose and glutamine to provide energy for repair of radiation damage. Glutaminolysis and glycolysis were inhibited by aminooxyacetic acid (AOAA) and 2-deoxyglucose (2DG), respectively, to inhibit metabolism of these substrates in order to determine the effect on radiation response of CHO...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098142419
更新日期:1998-03-01 00:00:00
abstract:PURPOSE:To determine the contribution of intra-alveolar cells as opposed to cells fixed in the interstitium in the development of radiation-induced lung injury. MATERIALS AND METHODS:C3H/HeN mice were irradiated to the thorax with various doses of radiation. The cellular composition and cytokine production were assess...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/0955300031000076894
更新日期:2003-03-01 00:00:00
abstract:PURPOSE:To calculate survival of human haemopoietic stem cells irradiated by alpha particles from 149Tb (initial energy 3.97 MeV) and 211At (average initial energy 6.87 MeV). METHODS:Following as closely as possible radiobiological data, Monte Carlo methods were used to calculate passages of alpha particles (originati...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141771
更新日期:1998-07-01 00:00:00
abstract::Context: Some individuals are reporting being sensitive to electromagnetic fields. They report some unspecific symptoms like headeache, sleep disruption, heart palpitations, itching, etc. The lack of scientific objective links between EHS symptoms and EMF exposure renders the diagnosis of EHS more complex. Purpose: Ou...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1569776
更新日期:2019-06-01 00:00:00
abstract::Male SAS/4 mice were injected i.v. with 6.6 kBq 239Pu-citrate. After 1 or 24 h a single i.p. injection of 15 or 30 mumol kg-1 or repeated (three or four) daily injections of 30 mumol kg-1 of tetra-THB-spermine were given, and at 4 or 7 days Pu retention was measured in liver, kidneys and femur. Besides tetra-THB-sperm...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914550141
更新日期:1989-01-01 00:00:00
abstract:PURPOSE:To investigate the p53 ionizing radiation-induced response, G1/S cell-cycle block and cytogenetic damage in Nijmegen breakage syndrome (NBS) cells characterized by different haplotypes. MATERIAL AND METHODS:Lymphoblastoid cell lines derived from three normals, five NBS and two ataxia telangiectasia (AT) indivi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099140221
更新日期:1999-05-01 00:00:00
abstract:PURPOSE:To ask whether highly metastatic sublines show more marked low-dose hyper-radiosensitivity (HRS) response than poorly metastatic ones. MATERIALS AND METHODS:The progressive (PRO) subline showing tumourigenicity and metastatic potential and the regressive (REG) subline showing neither tumourigenicity nor metast...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802195331
更新日期:2008-07-01 00:00:00
abstract:PURPOSE:To assess changes in mitochondrial morphology and mitophagy induced by simulated sunlight irradiation (SSI) and how these changes are modulated by mitochondrial activity and energy source. MATERIALS AND METHODS:Human malignant amelanotic melanoma A375 cells were pre-treated with either a mitochondrial activity...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.556175
更新日期:2011-05-01 00:00:00
abstract::Diploid elongated spermatids of mice were enriched by flow cytometry and cell sorting using a new type of sorter (Partec). The sorted abnormal spermatids were identified morphologically and by nuclear area integration. The radiation-induced increase in the frequency of diploid elongated spermatids was monitored with t...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914550841
更新日期:1989-05-01 00:00:00
abstract:PURPOSE:The impact of the damage distribution to cellular survival and chromosomal aberrations following high Linear Energy Transfer (LET) radiation was investigated. MATERIALS AND METHODS:High LET iron-ions (500 MeV/n, LET 200 keV/μm) were delivered to G1-phase synchronized Chinese Hamster Ovary (CHO) cells located a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.767990
更新日期:2013-06-01 00:00:00
abstract:PURPOSE:To summarize current knowledge regarding mechanisms of radiation-induced skin injury and medical countermeasures available to reduce its severity. Advances in radiation delivery using megavoltage and intensity modulated radiation therapy have permitted delivery of higher doses of radiation to well-defined tumor...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.3109/09553002.2013.765055
更新日期:2013-05-01 00:00:00
abstract::Evidence for the roles of proteins and metal ions in the microheterogeneity of DNA damage is reviewed. Decondensation of chromatin in hypotonic buffers markedly sensitizes the DNA to radiation, while treatment of nuclei with hypertonic buffers strips the DNA of histones and other nuclear proteins and enhances the radi...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009414551561
更新日期:1994-11-01 00:00:00
abstract::Electron transfer to 5-bromouracil (5-BrU) from nucleobase (N) electron adducts (and their protonated forms) has been studied by product analysis and pulse radiolysis. When an electron is transferred to 5-BrU, the ensuing 5-BrU radical anion rapidly loses a bromide ion; the uracilyl radical thus formed reacts with add...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214552451
更新日期:1992-11-01 00:00:00
abstract:PURPOSE:To determine the relationships between the frequencies of radiation-induced chromosomal alterations and the extent of apoptosis in G0 human lymphocytes. MATERIAL AND METHODS:G0 human peripheral blood lymphocytes (HPBL) were X or gamma-irradiated, in the presence or absence of the repair inhibitor cytosine arab...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000500303690
更新日期:2005-08-01 00:00:00
abstract:PURPOSE:To study cell damage and possible apoptosis in K562 human erythroleukemic cells exposed for 2 h to an extremely low frequency (ELF) 50 Hz sinusoidal magnetic field with a magnetic induction of either 1 or 5 mT using high resolution proton nuclear magnetic resonance (1H-NMR) spectroscopy. MATERIALS AND METHODS:...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000500102985
更新日期:2005-02-01 00:00:00
abstract:PURPOSE:In a previous study, we found that exposure to a 50 Hz magnetic field (MF) could activate stress-activated protein kinase (SAPK) and P38 mitogen-activated protein (MAP) kinase (P38 MAPK) in Chinese hamster lung (CHL) fibroblast cells, and simultaneous exposure to a 'noise' MF of the same intensity inhibited the...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000801998875
更新日期:2008-05-01 00:00:00
abstract::Cultures of JU-56 cells were irradiated in either G1 or G2 and examined either in their first post-irradiation metaphase (in diploids) or in their second post-irradiation metaphase (in colcemid-induced tetraploids). The timing of fixation, together with tritiated thymidine pulse labelling, allowed selection for scorin...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314552131
更新日期:1993-06-01 00:00:00