Abstract：
:Cultures of JU-56 cells were irradiated in either G1 or G2 and examined either in their first post-irradiation metaphase (in diploids) or in their second post-irradiation metaphase (in colcemid-induced tetraploids). The timing of fixation, together with tritiated thymidine pulse labelling, allowed selection for scoring only metaphases of cells that were in the G1 or G2 phase of the cell cycle during irradiation. With G1 irradiation it was found that many of the aberrations observed at the first division were not present (as derivatives) at the second division, and also that new aberrations were found at the second division, which were not derived from aberrations at the first division. Clonogenic survival was also measured in populations of cells irradiated in G1. It was found that cells containing chromosomal aberrations at the first division were not numerous enough to explain lack of survival. When frequencies of aberrations following G2 irradiation scored at the second division were compared with those scored at the first, there was a significant increase in dicentrics as compared with their progenitor asymmetrical chromatid interchanges, and of mirror-image dicentrics as compared with their progenitor sister unions. A substantial number of sister unions were also observed at the second division. We conclude that some aberrations are lost during the interphase between the first and the second post-irradiation metaphase and that new chromosomal aberrations arise during the second post-irradiation interphase.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Moore RC,Bender MAdoi
10.1080/09553009314552131subject
Has Abstractpub_date
1993-06-01 00:00:00pages
731-41issue
6eissn
0955-3002issn
1362-3095pii
YPV2ET2C5Q0A4L0Vjournal_volume
63pub_type
杂志文章abstract：:Only a small percentage of the DNA damage set by ionizing radiation in the living cell manifests itself as lethal. It is now increasingly accepted that clustered lesions may constitute the kind of damage that the repair enzymes cannot adequately deal with. The question is raised as to whether damage amplification reac...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414551501
更新日期：1994-11-01 00:00:00
abstract：PURPOSE:To determine the relative toxicity of alpha- and beta-radiations under conditions of controlled temporal and spatial dose distribution. METHODS:Fused aluminosilicate particles were radiolabelled with either (45)Ca (a beta-emitter) or (242)Cm (an alpha-emitter). These were injected into CBA/Ca mice to give life...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553000903564075
更新日期：2010-04-01 00:00:00
abstract：PURPOSE:To determine the relationships between the frequencies of radiation-induced chromosomal alterations and the extent of apoptosis in G0 human lymphocytes. MATERIAL AND METHODS:G0 human peripheral blood lymphocytes (HPBL) were X or gamma-irradiated, in the presence or absence of the repair inhibitor cytosine arab...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000500303690
更新日期：2005-08-01 00:00:00
abstract：:Nitrous oxide reacts during irradiation to increase the yield of .OH, a radical many believe to be a major cause of lethality. Logically, one would expect N2O to be a radiation sensitizer. In some instances it is, while in others it is not. In some cases we can explain why N2O fails to sensitize; factors such as dose ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550631
更新日期：1991-03-01 00:00:00
abstract：:Radioiodine ((131)I) induced a dose- and time-dependent increase in DNA single-strand breaks (DNA-ssb) in human (G0) mononuclear blood cells (MNC) in vitro. Incubation of MNC with 22MBq (131)I/ml at 4 degrees C caused a linear, time-dependent induction of DNA-ssb (increase in elution rate: 24.7 x 10(-3) h(-1) per 100 ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097143103
更新日期：1997-11-01 00:00:00
abstract：:The radioprotective agent WR-2721 is dephosphorylated to the free thiol form WR-1065 in vivo. The effects of WR-2721, WR-1065 and reduced glutathione on a mitochondrial lipid peroxidation system were compared. WR-2721 had no effect on mitochondrial lipid peroxidation in vitro, and could not prevent the inactivation of...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014552611
更新日期：1990-03-01 00:00:00
abstract：PURPOSE:Caffeine (1,3,7-trimethyl xanthine), a dietary component, has been shown to have widely varying effects on DNA damage induced by UV and ionizing radiation, depending upon pre- or post-irradiation administration and its concentration. Caffeine administered post-UV irradiation is known to inhibit enzymatic repair...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110034649
更新日期：2001-05-01 00:00:00
abstract：:Radiation-induced DNA double-strand breaks (dsb) were studied in Ehrlich ascites tumour cells (EATC) by sedimentation in neutral sucrose gradients at low centrifuge speed. Dsb induction was found to be linear with dose with a frequency of: ndsbmr-1D-1 = (11.7 +/- 2) x 10(-12)Gy-1 for 140 kV X-rays and ndsbmr-1D-1 = (1...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008814552201
更新日期：1988-11-01 00:00:00
abstract：PURPOSE:To investigate the mechanism of methylene blue-induced radiosensitization in Escherichia coli cells. MATERIALS AND METHODS:Bacteriophage lambda15 was irradiated with X-rays in the presence or absence of methylene blue (MB) and infected into bacteria with different repair capabilities preincubated with or witho...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050134528
更新日期：2000-09-01 00:00:00
abstract：:Male SAS/4 mice were injected i.v. with 6.6 kBq 239Pu-citrate. After 1 or 24 h a single i.p. injection of 15 or 30 mumol kg-1 or repeated (three or four) daily injections of 30 mumol kg-1 of tetra-THB-spermine were given, and at 4 or 7 days Pu retention was measured in liver, kidneys and femur. Besides tetra-THB-sperm...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008914550141
更新日期：1989-01-01 00:00:00
abstract：PURPOSE:To investigate whether radiation survival of cells irradiated aerobically in the oxygen-sensitive restriction point in late G1 is dependent on where in the cell cycle the cells first were rendered hypoxic. MATERIALS AND METHODS:Human cervix carcinoma, NHIK 3025 cells, were synchronized and rendered hypoxic whi...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098141366
更新日期：1998-10-01 00:00:00
abstract：PURPOSE:We already reported that levels of interferon (IFN)-γ have been shown to be markedly reduced in mice seven weeks after irradiation, resulting in a T helper (Th) 1/Th2 imbalance. To investigate whether the single or fractionated γ-irradiation induced an immune imbalance, we analysed the Th1-related immune respon...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2011.542540
更新日期：2011-05-01 00:00:00
abstract：PURPOSE:The aim is to assess the change with age and disease of the oxygen concentration within the coronary artery walls. MATERIALS AND METHODS:In an accompanying paper, Part A, the age-dependent morphology and composition for the wall layers of normal and diseased coronary arteries were developed from published data...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000802389645
更新日期：2008-10-01 00:00:00
abstract：PURPOSE:To study the relationship between cell reproductive death and exchange frequency in SW-1573 human lung tumour cells with and without incorporated iodo-deoxyuridine (IdUrd) following irradiation of plateau-phase cultures with y-rays. METHOD:Linear-quadratic (LQ) analysis was performed for the data on clonogenic...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099140474
更新日期：1999-03-01 00:00:00
abstract：PURPOSE:The present work summarises the history and current status of research into the importance of cancer stem cells for radiobiological research and for clinical radiation oncology. An effort is made to differentiate clonogenicity from stemness of cancer cells. CONCLUSION:In radiooncology, cancer stem cells have b...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553000902836404
更新日期：2009-05-01 00:00:00
abstract：PURPOSE:To evaluate the characteristic features of mechanical responses and the membrane potential changes induced by repetitive pulsed electromagnetic field (PEMF, 50 Hz, 5 mT) in thoracic aorta rings obtained from streptozotocin-induced diabetic and healthy control rats to determine if PEMF could ameliorate problems ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000903009522
更新日期：2009-08-01 00:00:00
abstract：PURPOSE:In a previous study, we found that exposure to a 50 Hz magnetic field (MF) could activate stress-activated protein kinase (SAPK) and P38 mitogen-activated protein (MAP) kinase (P38 MAPK) in Chinese hamster lung (CHL) fibroblast cells, and simultaneous exposure to a 'noise' MF of the same intensity inhibited the...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000801998875
更新日期：2008-05-01 00:00:00
abstract：PURPOSE:To evaluate the impact of oral administration of purslane (Portulaca oleracea) extract or fish oil and their co-treatments in the modulation of radiation-induced damage. MATERIAL AND METHODS:Purslane (P) (400 mg/kg body weight) or fish oil (Fo) (60 mg/kg body weight) was administrated to male albino rats via g...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2014.926040
更新日期：2014-12-01 00:00:00
abstract：PURPOSE:To compare experimental yields of single strand breaks (SSB) and double strand breaks (DSB) induced in plasmid DNA in aqueous solution by alpha-particles and Al(K) ultrasoft X-rays (USX) with the corresponding yields, generated via computer simulations, for a range of mean diffusion distances of the hydroxyl ra...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110069308
更新日期：2001-10-01 00:00:00
abstract：PURPOSE:Cartilage degradation usually results as a consequence of inflammatory processes in the joints. To study this phenomenon experimentally, adjuvant-induced arthritis (AIA) was used as a model of chronic inflammation under the influence of irradiation. The potential chondroprotective effect of 13% aqueous extract ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2011.530337
更新日期：2011-03-01 00:00:00
abstract：:Determinations of cell sensitivity in terms of survival fraction after doses employed in clinical radiation therapy, say 1-3 Gy, are of increasing interest to clinicians as they provide direct experimental data which can be employed without reference to models of cell inactivation. SF2 values are expected ultimately t...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553008914551971
更新日期：1989-11-01 00:00:00
abstract：PURPOSE:To propose an improved version of RADACK, a stochastic simulation of radiolytic attack on DNA, that takes into account the reactivity of each amino acid of a specifically bound protein with hydroxyl radicals. To apply it to the natural lactose operator-repressor complex taking advantage of recently reported str...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/0955300031000137788
更新日期：2003-06-01 00:00:00
abstract：PURPOSE:As the most recent epidemiological studies provide no definite conclusions about the effects of 50/60 Hz magnetic fields (MFs) on the incidence of leukaemia in humans, animal models in a well-controlled environment are useful for evaluating the possibility of an association between MFs and leukaemia. The presen...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050029011
更新日期：2000-06-01 00:00:00
abstract：:Purpose: Radiotherapy prescription dose and dose fractionation protocols vary little between individual patients having the same tumor grade and stage. To personalize radiotherapy a predictive model is needed to simulate radiation response. Previous modeling attempts with multiple variables and parameters have been sh...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1589013
更新日期：2019-10-01 00:00:00
abstract：PURPOSE:Animal models that accurately reflect human responses to radiation injury are needed for advanced mechanistic investigation and development of effective therapeutics. The rabbit is an established animal model accepted by the FDA for studies of cardiovascular disease, lipid metabolism, the development of anticoa...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1820606
更新日期：2020-10-26 00:00:00
abstract：PURPOSE:The linear nonthreshold (LNT) model has been used in radiation protection for over 40 years and has been hotly debated. It relies heavily on human epidemiology, with support from radiobiology. The scientific underpinnings include NCRP Report No. 136 ('Evaluation of the Linear-Nonthreshold Dose-Response Model fo...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2017.1328750
更新日期：2017-10-01 00:00:00
abstract：PURPOSE:Multicellular tumour spheroids are being used with increasing frequency in various aspects of tumour biology, including studies dealing with radiation biology. This review attempts to outline recent studies using these three-dimensional systems in radiation biology with particular reference made to papers testi...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/095530099139845
更新日期：1999-07-01 00:00:00
abstract：PURPOSE:To estimate gamma-ray doses received by Hiroshima atomic bomb survivors using electron spin resonance (ESR) of tooth enamel and to compare the results with cytogenetic data. MATERIALS AND METHODS:Tooth enamel ESR was performed for 100 teeth donated from 69 Hiroshima survivors, and conventional cytogenetic exam...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098141870
更新日期：1998-06-01 00:00:00
abstract：PURPOSE:To examine the relationship between cell killing and residual chromatin breaks after irradiation with qualitatively different types of radiation in six human cell lines. MATERIALS AND METHODS:Six human tumour cell lines and normal human cells were irradiated with 200 kV X-rays and two carbon-ion beams accelera...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050134429
更新日期：2000-09-01 00:00:00
abstract：PURPOSE:To ask whether highly metastatic sublines show more marked low-dose hyper-radiosensitivity (HRS) response than poorly metastatic ones. MATERIALS AND METHODS:The progressive (PRO) subline showing tumourigenicity and metastatic potential and the regressive (REG) subline showing neither tumourigenicity nor metast...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000802195331
更新日期：2008-07-01 00:00:00
