Abstract：
:Determinations of cell sensitivity in terms of survival fraction after doses employed in clinical radiation therapy, say 1-3 Gy, are of increasing interest to clinicians as they provide direct experimental data which can be employed without reference to models of cell inactivation. SF2 values are expected ultimately to prove valuable as response predictors. Even so, SF2 values would surely be combined with other predictors also under development to give the best feasible estimate of response of tumor and normal tissue. There are, however, several concerns with the SF2 data currently available. These include: SF2 depends upon the cell system employed (established cell lines vs primary cultures) and the method of assaying survival fraction (colony formation vs population growth); dose-response curves for inactivation of tumors characterized by the reported distribution of SF2 values are, in many instances, not close to those judged to obtain in clinical practice; the broad distribution of SF2 values indicates a rather flatter dose-response curve for tumor control or normal tissue than seems true from clinical experience. There appears to be a potential for clinical gain by determination of sensitivity of normal tissues in order to identify patients who are of increased sensitivity (for example heterozygotes for AT, 5-oxoprolinuria, etc.). Although the absolute SF2 values obtained by current technologies of culturing human cells often appear to be poorly related to values expected from observed radiation response in patients, intensive research on cell viability assays will almost certainly yield more realistic results.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Suit HD,Baumann M,Skates S,Convery Kdoi
10.1080/09553008914551971subject
Has Abstractpub_date
1989-11-01 00:00:00pages
725-37issue
5eissn
0955-3002issn
1362-3095pii
QY5YCK1EP305G7RVjournal_volume
56pub_type
杂志文章,评审abstract：:In a series of studies to investigate activation energies and thermal enhancements of various chemotherapeutic agents the effect of 5-fluoruracil (5FU), an antimetabolite, on murine tumour cells was studied at elevated temperatures. Animal tumours were early generation isotransplants of a spontaneous fibrosarcoma, FSa...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550211
更新日期：1991-01-01 00:00:00
abstract：PURPOSE:Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments describ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138844
更新日期：2000-02-01 00:00:00
abstract：PURPOSE:To study the influence of TGF-beta1 and TGF-beta-neutralizing antibodies on the clonogenic activity and terminal differentiation of rat lung fibroblasts following radiation exposure. MATERIAL AND METHODS:Early passage rat lung fibroblasts were used in this study. Colony formation assays were applied to determi...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138501
更新日期：2000-04-01 00:00:00
abstract：:The studies of the late effects of atomic-bomb (A-bomb) radiation on the immune system were started about 20 years after the bombings in 1945. The most remarkable late effects of radiation are the functional and quantitative abnormalities of T and B cells in survivors exposed to high doses (> or = 1.0 Gy). Abnormaliti...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009514551491
更新日期：1995-11-01 00:00:00
abstract：PURPOSE:To examine the heat sensitivity of DNA-dependent protein kinase (DNA-PK) activity in a variety of cultured mouse, hamster and human cell lines. MATERIALS AND METHODS:Eight cell lines, which have been routinely used in our laboratory, were examined. Cells were heated at 44.0 +/- 0.05 degrees C and DNA-PK activi...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000310001596959
更新日期：2003-08-01 00:00:00
abstract：:Electron transfer to 5-bromouracil (5-BrU) from nucleobase (N) electron adducts (and their protonated forms) has been studied by product analysis and pulse radiolysis. When an electron is transferred to 5-BrU, the ensuing 5-BrU radical anion rapidly loses a bromide ion; the uracilyl radical thus formed reacts with add...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214552451
更新日期：1992-11-01 00:00:00
abstract：:Most estimates of the radiation dose lethal to 50 per cent of a human population are based on historical data taken from well-known experiences reflecting inadequately known physical and biological conditions, or from medical procedures where individual patients received advantages of modern clinical care. It has been...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008914550481
更新日期：1989-03-01 00:00:00
abstract：:The in vitro cell survival of a human cervix carcinoma cell line (HX156c) has been assessed using 60Co gamma-rays administered at either high (150 cGy/min) or low (3.2 cGy/min) dose rate. Recovery during low dose-rate irradiation was observed; the dose reduction factor at 10(-2) cell kill for 150 versus 3.2 cGy/min wa...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008814551671
更新日期：1988-08-01 00:00:00
abstract：:SR 2508 (etanidazole) (1 mM) or buthionine sulphoximine (BSO, 50 microM) or both drugs together did not radiosensitize oxic V79 Chinese hamster cells irradiated at either an acute dose rate (2.35 Gy/min) or at a low dose rate (0.117 Gy/min). BSO pretreatment (15 h at 37 degrees C) depleted cellular glutathione (GSH) t...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550121
更新日期：1991-01-01 00:00:00
abstract：:DNA neutral (pH 9.6) filter elution was used to measure radiation-induced DNA double-strand break (dsb) frequencies in eight human squamous cell carcinoma cell lines with radiosensitivities (D0) ranging from 1.07 to 2.66 Gy and D values ranging from 1.46 to 4.08 Gy. The elution profiles of unirradiated samples from mo...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114551211
更新日期：1991-06-01 00:00:00
abstract：PURPOSE:The aim of this work is to study the relative damaging effects of DNA-incorporated radionuclides by analyzing the behavior of the liquid-scintillation counting efficiency in volumes of nanometer size. METHODS:A liquid scintillation counter can detect changes in the micelle size when different percentages of an...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000802460156
更新日期：2008-12-01 00:00:00
abstract：PURPOSE:To analyze the direct and transgenerational effects of exposure to low-dose 1 GHz (mobile phone/wireless telecommunication range) and 10 GHz (radar/satellite communication range) radiofrequency electromagnetic fields (RF-EMF) on the motility of ciliates Spirostomum ambiguum. MATERIALS AND METHODS:S. ambiguum w...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2014.868612
更新日期：2014-03-01 00:00:00
abstract：PURPOSE:The detection of long-term persistent chromosome aberrations in circulating haemopoietic stem cells after accidental radiation exposure. MATERIAL AND METHODS:Peripheral blood samples from highly exposed persons were collected 7-25 years after the radiation accidents in Moscow (1971), Kazan (1975) and Chernobyl...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099139395
更新日期：1999-10-01 00:00:00
abstract：:Studies of protein phosphorylation in the second cell cycle of mouse embryos were assessed by [32P]-incorporation and one- and two-dimensional electrophoresis. Unirradiated and X-irradiated embryos were compared. X-irradiation in the G2 phase led to a block in this cell cycle phase. The phosphorylation of proteins wit...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114552351
更新日期：1991-09-01 00:00:00
abstract：PURPOSE:To examine the relationship between cell killing and residual chromatin breaks after irradiation with qualitatively different types of radiation in six human cell lines. MATERIALS AND METHODS:Six human tumour cell lines and normal human cells were irradiated with 200 kV X-rays and two carbon-ion beams accelera...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050134429
更新日期：2000-09-01 00:00:00
abstract：:Male SAS/4 mice were injected i.v. with 6.6 kBq 239Pu-citrate. After 1 or 24 h a single i.p. injection of 15 or 30 mumol kg-1 or repeated (three or four) daily injections of 30 mumol kg-1 of tetra-THB-spermine were given, and at 4 or 7 days Pu retention was measured in liver, kidneys and femur. Besides tetra-THB-sperm...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008914550141
更新日期：1989-01-01 00:00:00
abstract：:Chinese hamster ovary cells were exposed to various heat treatments followed by X-irradiation, and the induction and repair of DNA strand breaks was studied using the alkaline unwinding technique. Heat treatments alone were found to cause DNA strand breakage only for temperatures greater than or equal to 43 degrees C,...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214550851
更新日期：1992-02-01 00:00:00
abstract：PURPOSE:To study the natural radionuclides in the freshwater fish samples around the uranium mining areas of Bagjata and Banduhurang, East Singhbhum, Jharkhand, India. MATERIALS AND METHODS:The naturally occurring radioisotopes of uranium, U(nat), consisting of (234)U, (235)U and (238)U; (226)Ra, (230)Th and (210)Po w...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.492490
更新日期：2010-11-01 00:00:00
abstract：:Radiation-induced DNA double-strand breaks (dsbs) were measured in CHO-K1 cells by means of an experimental approach involving constant-field gel electrophoresis and densitometric scanning of ethidium bromide stained gels. For X-irradiation, an induction efficiency of 36 +/- 5 dsbs (Gy x cell)-1 was determined. With t...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514551051
更新日期：1995-08-01 00:00:00
abstract：:Samples of human rib were collected at autopsy and 20 were selected for marrow fat cell measurement, representing an age range of 16-96 years. The mean diameter of fat cells in red bone marrow of human rib was found to increase from around 48 microns at ages 16-29 years to around 65 microns at ages 82-96 years. There ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514551681
更新日期：1995-12-01 00:00:00
abstract：PURPOSE:To assess health, growth and reproductive success of mammals exposed for multiple generations to levels of radium-226 known to occur in environments surrounding uranium mines and mills in Canada. METHODS:The study consisted of a control group and four treatment groups each containing 40 mice (20 males and 20 f...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2015.1028598
更新日期：2015-07-01 00:00:00
abstract：PURPOSE:5-Formyluracil (5-foU) is a major derivative of thymine produced in DNA by ionizing radiation and various chemical oxidants. It has been previously shown that 5-foU in template DNA directs misincorporation of nucleotides by DNA polymerases during in vitro DNA synthesis. The present experiments were designed to ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530001453113
更新日期：2001-01-01 00:00:00
abstract：:Reasonable expectations, based on the normal physiology of the human fetus, cast new light on the possibilities of extrapolation from the finding of severe mental retardation (SMR) in Japanese bomb survivors after maternal exposure to 1.8-5.5 Gy T65DR Gy. After such large exposures the fetal haematopoietic tissues (DS...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014552051
更新日期：1990-10-01 00:00:00
abstract：:Genetic analysis was performed on 72 Italian A-T families ascertained through 91 probands. The frequency of the A-T gene was estimated through Dahlberg's formula, which uses the frequencies of first cousin marriages among the patients' parents and in the general population were obtained from the collection of Catholic...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：PURPOSE:This review will examine the role of phosphatases in cancer cell signalling and also outline emerging findings regarding the influence of phosphatases on tumor cell survival after ionising radiation. CONCLUSION:The exposure of tumour cells to clinically relevant doses of ionising radiation causes DNA damage an...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.3109/09553000903232827
更新日期：2009-11-01 00:00:00
abstract：PURPOSE:The main aim of the present study is to gain mechanistic insights into the modulating effect of molecular hydrogen on the γ-radiation-induced alteration pathways of DNA nucleobases. MATERIALS AND METHODS:Aerated aqueous solutions of calf thymus DNA were exposed to a (60)Co source at doses ranging from 0 to 55 ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2016.1206234
更新日期：2016-09-01 00:00:00
abstract：PURPOSE:The need to determine a reliable relative biological effectiveness (RBE) value for alpha exposures has become important as reports remain controversial. Although a radiation-weighting factor of 20 has been designated for alpha particles, uncertainty exists on realistic value of the RBE of alpha radiation. The a...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2015.1047986
更新日期：2015-08-01 00:00:00
abstract：PURPOSE:Exposure to ionizing radiation induces plant defenses by regulating the expression of response genes. The systemic acquired resistance deficient 1 (SARD1) is a key gene in plant defense response. In this study, the function of Oryza sativa SARD1 (OsSARD1) was investigated after exposure of seeds/plants to ioniz...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2017.1297901
更新日期：2017-07-01 00:00:00
abstract：PURPOSE:To assess changes in mitochondrial morphology and mitophagy induced by simulated sunlight irradiation (SSI) and how these changes are modulated by mitochondrial activity and energy source. MATERIALS AND METHODS:Human malignant amelanotic melanoma A375 cells were pre-treated with either a mitochondrial activity...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2011.556175
更新日期：2011-05-01 00:00:00
abstract：:BW12C, which was developed as an agent for the treatment of sickle cell anaemia, increases the binding of oxygen to haemoglobin and hence reduces the availability of oxygen to tissues. Due to these changes in oxygen availability BW12C could act as a protector against radiation-induced injury to normal tissues. In this...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550921
更新日期：1991-04-01 00:00:00
