Abstract:
PURPOSE:To resolve the difficulty in counting merged DNA damage foci in high-LET (linear energy transfer) ion-induced patterns. MATERIALS AND METHODS:The analysis of patterns of RIF (radiation-induced foci) produced by high-LET Fe and Ti ions were conducted by using a Monte Carlo model that combines the heavy ion track structure with characteristics of the human genome on the level of chromosomes. The foci patterns were also simulated in the maximum projection plane for flat nuclei. RESULTS:The model predicts the spatial and genomic distributions of DNA DSB (double-strand breaks) in a cell nucleus for a particular dose of radiation. We used the model to do analyses for three irradiation scenarios: (i) The ions were oriented perpendicular to the flattened nuclei in a cell culture monolayer; (ii) the ions were parallel to that plane; and (iii) round nucleus. In the parallel scenario we found that the foci appeared to be merged due to their high density, while, in the perpendicular scenario, the foci appeared as one bright spot per hit. The statistics and spatial distribution of regions of densely arranged foci, termed DNA foci chains, were predicted numerically using this model. Another analysis was done to evaluate the number of ion hits per nucleus, which were visible from streaks of closely located foci. CONCLUSIONS:We showed that DSB clustering needs to be taken into account to determine the true DNA damage foci yield, which helps to determine the DSB yield. Using the model analysis, a researcher can refine the DSB yield per nucleus per particle. We showed that purely geometric artifacts, present in the experimental images, can be analytically resolved with the model, and that the quantisation of track hits and DSB yields can be provided to the experimentalists who use enumeration of radiation-induced foci in immunofluorescence experiment using proteins that detect DNA damage.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Ponomarev AL,Huff J,Cucinotta FAdoi
10.3109/09553001003717175subject
Has Abstractpub_date
2010-06-01 00:00:00pages
507-15issue
6eissn
0955-3002issn
1362-3095journal_volume
86pub_type
杂志文章abstract:PURPOSE:To obtain compounds that will effectively reduce the fixation of uranium in its main target organs: bone and kidney. There is an urgent need for a chelating agent that is suitable and available for human use. MATERIALS AND METHODS:The efficacy of ethane-1-hydroxy-1,1-bisphosphonate (EHBP), already in use as a ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139331
更新日期:1999-11-01 00:00:00
abstract::Purpose: Radiotherapy prescription dose and dose fractionation protocols vary little between individual patients having the same tumor grade and stage. To personalize radiotherapy a predictive model is needed to simulate radiation response. Previous modeling attempts with multiple variables and parameters have been sh...
journal_title:International journal of radiation biology
pub_type: 杂志文章
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更新日期:2019-10-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550211
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000600562229
更新日期:2005-11-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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abstract::DNA double-strand break (dsb) induction in diploid yeast was measured by neutral sucrose sedimentation after exposure to very heavy ions with values of linear energy transfer (LET) ranging from about 300 to 11500 ke V/microns. Linear fluence dependencies were found in all cases from which dsb production cross-sections...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214552121
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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更新日期:2008-11-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551571
更新日期:1993-10-01 00:00:00
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pub_type: 杂志文章
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abstract::We have re-examined the effect of detergent type, pH and temperature of lysis on the shape of the DNA elution dose response under non-denaturing conditions using plateau-phase CHO cells. Results practically identical to those previously published (Okayasu and Iliakis, 1989) were obtained, with a 1 h incubation at 60 d...
journal_title:International journal of radiation biology
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pub_type: 杂志文章
doi:10.1080/095530097144382
更新日期:1997-01-01 00:00:00
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journal_title:International journal of radiation biology
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doi:10.3109/09553002.2011.530337
更新日期:2011-03-01 00:00:00
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更新日期:2000-03-01 00:00:00
abstract:PURPOSE:To investigate the mechanism of methylene blue-induced radiosensitization in Escherichia coli cells. MATERIALS AND METHODS:Bacteriophage lambda15 was irradiated with X-rays in the presence or absence of methylene blue (MB) and infected into bacteria with different repair capabilities preincubated with or witho...
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pub_type: 杂志文章
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314550201
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journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2017.1332440
更新日期:2017-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1080/095530098142518
更新日期:1998-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1080/095530000138600
更新日期:2000-04-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2017.1328750
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abstract::This paper presents a summary of our understanding to date of the formation of DNA strand breaks induced by highly energetic particle beams (high-LET radiation). We have compared our own recent data on the formation of strand breaks induced in DNA in an aqueous solution with our previous data and those of others avail...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
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更新日期:1990-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1080/09553000050201073
更新日期:2000-12-01 00:00:00
abstract::One-cell mouse embryos were irradiated with X-rays or neutrons. Analysis of the first, second, and third postradiation mitoses revealed that the yields of structural aberrations increased linearly after exposure to both radiation qualities. For X-rays the aberration frequency decreased from the first to the third mito...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814551771
更新日期:1988-09-01 00:00:00
abstract::A radionuclide release assay for measuring the in vitro kinetics of cell death has been developed. CHO cells were labelled for 24 h with 3.0 hBq/ml of [125I] iododeoxyuridine (125IUdR) and the fate of the labelled cells and their progeny was monitored at daily intervals by measuring the rate of 125I release. Prelabell...
journal_title:International journal of radiation biology
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doi:10.1080/09553009114550361
更新日期:1991-02-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009414551761
更新日期:1994-11-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110053873
更新日期:2001-07-01 00:00:00
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journal_title:International journal of radiation biology
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doi:10.1080/09553000110043711
更新日期:2001-06-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00
abstract::In mice, external X- or gamma-irradiation may induce thymic lymphomas or myeloid leukaemias, while bone-seeking alpha-emitters may induce osteosarcomas and, to a lesser extent, acute myeloid leukaemia. The present paper aims to review briefly some of the experimental data with respect to the molecular mechanisms under...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009014550851
更新日期:1990-04-01 00:00:00
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pub_type: 杂志文章
doi:10.3109/09553002.2013.791407
更新日期:2013-09-01 00:00:00