Abstract:
PURPOSE:The low dose (<1 Gy) survival curve of mammalian cells is characterized by a region of hypersensitivity (HRS) followed by increased resistance (IRR). Above 1 Gy, the survival response can be described with a smooth downward-bending curve. Indirect evidence has indicated that the IRR response might reflect an induced radioresistance triggered by DNA damage. The work reported here provides experimental evidence that consolidates this hypothesis. MATERIALS AND METHODS:Clonogenic survival of V79-379A cells was measured using automated microscopy (DMIPS cell analyser) in the presence or absence of three known modifiers of DNA repair processes over the X-ray dose range 0-1 Gy. RESULTS:3-Aminobenzamide (5 microM), a potent inhibitor of poly(ADP ribose)-polymerase, inhibited the development of increased radioresistance as indicated by a statistically significant reduction in an RBE from 3.537 (+/-SEM (0.139)) to 2.168 (+/-0.191) at an X-ray dose of 1 Gy, implying an involvement of DNA repair pathways that require poly(ADP ribose)-polymerase in the IRR response. In contrast, novobiocin (350 microM), an inhibitor of topoisomerase II, did not inhibit the development of increased radioresistance (RBE 3.650 (+/-0.192) to 3.322+/-(0.156)) but eliminated low-dose hypersensitivity as measured by an increase in RBE from 2.508 (+/-0.536) to 1.135 (+/-0.057) at 0.04 Gy. Ara-A (120 microM), an inhibitor of DNA polymerase, sensitized cells at all doses. CONCLUSION:These data support the hypothesis that DNA repair processes are likely to be involved in the development of increased radioresistance and provide further evidence against a sensitive subpopulation explanation for the biphasic low-dose survival response.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Marples B,Joiner MCdoi
10.1080/095530000138646subject
Has Abstractpub_date
2000-03-01 00:00:00pages
305-12issue
3eissn
0955-3002issn
1362-3095journal_volume
76pub_type
杂志文章abstract:PURPOSE:To illustrate the progress in normal tissue radiation biology over the last five decades and its impact on radiotherapy. MATERIALS AND METHODS:Major milestones over the last 50 years and their consequences for radiation oncology are described: The identification of clonogenic cell survival and the (target) ste...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553000902985136
更新日期:2009-07-01 00:00:00
abstract:PURPOSE:To investigate the role of tumour versus non-tumour factors in the specific response to serotonin (5-HT) of tumour-feeding arterioles (TFA). MATERIALS AND METHODS:Using mouse models of intra-vital microscopy, the response to topical administration of 5-HT was studied in arterioles feeding tumours: fibrosarcoma...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141519
更新日期:1998-09-01 00:00:00
abstract::Cells use substrates such as glucose and glutamine to provide energy for repair of radiation damage. Glutaminolysis and glycolysis were inhibited by aminooxyacetic acid (AOAA) and 2-deoxyglucose (2DG), respectively, to inhibit metabolism of these substrates in order to determine the effect on radiation response of CHO...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098142419
更新日期:1998-03-01 00:00:00
abstract:PURPOSE:Melanoma is mainly treated by surgery and rarely with radiation because of the high radioresistance of this tumor. Nevertheless, radiotherapy is the preferred treatment modality for unresectable lesions and avoiding cosmetic disfigurement caused by surgical excision. This study investigated the therapeutic adva...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2017.1276309
更新日期:2017-05-01 00:00:00
abstract:PURPOSES:To estimate the ratio of risks for exposure to radon progeny relative to low-LET radiation based on human lung cancer data, taking account of possible time and age variations in radiation-induced lung cancer risk. MATERIALS AND METHODS:Fitting two sorts of time- and age-adjusted relative risk models to a case...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110095714
更新日期:2002-03-01 00:00:00
abstract:PURPOSE:The assessment of biological effects caused by radiation exposure has been currently carried out with the linear-quadratic (LQ) model as an extension of the linear non-threshold (LNT) model. In this study, we suggest a new mathematical model named as SeaSaw (SS) model, which describes proliferation and cell dea...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2021.1854487
更新日期:2020-12-15 00:00:00
abstract:PURPOSE:The long-term effect of low and moderate doses of ionizing radiation on the lens is still a matter of debate and needs to be evaluated in more detail. MATERIAL AND METHODS:We conducted a detailed histological analysis of eyes from B6C3F1 mice cohorts after acute gamma irradiation (60Co source; 0.063 Gy/min) at...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2021.1876951
更新日期:2021-01-19 00:00:00
abstract:PURPOSE:Static magnetic field (SMF) exposure was shown experimentally to beneficially affect the healing process. The aim of this randomized, controlled trial was to evaluate the pain-inhibitory effect of local exposure to SMF on temporomandibular disorders, on alveolitis and on aphta in the oral mucosa. MATERIALS AND...
journal_title:International journal of radiation biology
pub_type: 杂志文章,随机对照试验
doi:10.3109/09553002.2012.661916
更新日期:2012-05-01 00:00:00
abstract:PURPOSE:To investigate within the framework of a multilaboratory study the suitability of FISH chromosome painting to measure so-called stable translocations in peripheral lymphocytes of Mayak nuclear-industrial workers (from the Southern Urals) and their use for retrospective biodosimetry. MATERIALS AND METHODS:Chrom...
journal_title:International journal of radiation biology
pub_type: 杂志文章,多中心研究
doi:10.1080/09553000010018693
更新日期:2001-03-01 00:00:00
abstract::Purpose: To examine the effects of low-dose exposure to uranium with a systems biology approach, a multiscale high-throughput multi-omics analysis was applied with a protocol for chronic exposure to the rat kidney. Methods: Male and female rats were contaminated for nine months through their drinking water with a nont...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1577567
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:To test whether radiolysis-induced fragmentation in frozen aqueous protein solution is dependent on solvent access to the surface of the protein or to the molecular mass of the polypeptide chain. MATERIALS AND METHODS:60Co gamma-irradiation of three proteins at -78 degrees C: lysozyme, citrate synthase and alp...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138349
更新日期:2000-05-01 00:00:00
abstract:PURPOSE:Gene therapy combined with radiation has shown promising potential for the treatment of tumors. This paper aimed to clarify the synergistic effect of radiotherapy combined with the bladder cancer tissue-specific oncolytic adenovirus (Ad-PSCAE-UPII-E1A) on bladder cancer cells and to study the underlying synergy...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2017.1231942
更新日期:2017-02-01 00:00:00
abstract:PURPOSE:To construct a quantitative model of the radiation-induced bystander effect based on diffusion-type spreading of bystander signal communication between the hit and non-hit cells. Cell inactivation and induced oncogenic transformation by broad- and microbeam irradiation systems are considered. MATERIALS AND MET...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:PURPOSE:To evaluate the impact of oral administration of purslane (Portulaca oleracea) extract or fish oil and their co-treatments in the modulation of radiation-induced damage. MATERIAL AND METHODS:Purslane (P) (400 mg/kg body weight) or fish oil (Fo) (60 mg/kg body weight) was administrated to male albino rats via g...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.926040
更新日期:2014-12-01 00:00:00
abstract:PURPOSE:To understand the mechanisms of life-shortening due to early neoplastic death caused by chronic low dose-rate (LDR; 20 mGy/22 h/day) radiation which accumulates to a high dose (HD; 8 Gy) (LDR/HD) as reported previously. MATERIALS AND METHODS:Female B6C3F(1) mice were continuously exposed to LDR/HD gamma-rays u...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.545861
更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:Element-specific biokinetic models are used to reconstruct doses to systemic tissues from internal emitters. Typically, a systemic model for a radionuclide explicitly depicts only its dominant repositories. Remaining tissues and fluids are aggregated into a pool called Other tissue in which the radionuclide ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2018.1554923
更新日期:2018-12-04 00:00:00
abstract:PURPOSE:To investigate cell cycle effects and relative biological effectiveness (RBE) of α-particles from the clinically relevant radionuclide Astatine-211 ((211)At), using X-rays as reference radiation. Double-strand breaks (DSB), non-DSB clusters containing oxidised purines and clonogenic survival were investigated. ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.538127
更新日期:2011-04-01 00:00:00
abstract::Radiation-induced DNA double-strand breaks (dsb) were studied in Ehrlich ascites tumour cells (EATC) by sedimentation in neutral sucrose gradients at low centrifuge speed. Dsb induction was found to be linear with dose with a frequency of: ndsbmr-1D-1 = (11.7 +/- 2) x 10(-12)Gy-1 for 140 kV X-rays and ndsbmr-1D-1 = (1...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814552201
更新日期:1988-11-01 00:00:00
abstract::The effects of a low dose (0.1-20 mGy) preirradiation with X-rays followed by a higher dose (2 Gy) of the same radiation on the recovery of the genetic damage induced as dominant lethals in mature oocytes (stage 14) of different strains of Drosophila melanogaster were investigated. The response was shown to be depende...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414550501
更新日期:1994-04-01 00:00:00
abstract::Purpose: To summarize existing knowledge and to understand individual response to radiation exposure, the MELODI Association together with CONCERT European Joint Programme has organized a workshop in March 2018 on radiation sensitivity and susceptibility.Methods: The workshop reviewed the current evidence on this matt...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2019.1665209
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:Many studies have shown that exposure to radiofrequency radiation (RFR) could activate cellular signal transduction pathways. In the present research, we investigated the effects of exposure to a 1.8-GHz RFR at different intensities on epidermal growth factor (EGF) receptor clustering and phosphorylation in hum...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.634882
更新日期:2012-03-01 00:00:00
abstract::Radiologic accidents or terrorist acts involving radioactive material, as well as radiation exposure in medical or industrial procedures are potential sources of risk for human health. All these risks share a common element, exposure to ionizing radiation. The extent of ionizing radiation injury will depend on a numbe...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2019.1562254
更新日期:2019-05-01 00:00:00
abstract:PURPOSE:Ionizing radiation induces DNA damage, some of which are present in clusters, defined as two or more lesions within one to two helical turns of DNA by passage of a single radiation track. These clusters are thought to contribute to the detrimental effects of radiation, in part due to the compromised repair of c...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.899449
更新日期:2014-06-01 00:00:00
abstract:PURPOSE:To obtain compounds that will effectively reduce the fixation of uranium in its main target organs: bone and kidney. There is an urgent need for a chelating agent that is suitable and available for human use. MATERIALS AND METHODS:The efficacy of ethane-1-hydroxy-1,1-bisphosphonate (EHBP), already in use as a ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139331
更新日期:1999-11-01 00:00:00
abstract:PURPOSE:The impact of the damage distribution to cellular survival and chromosomal aberrations following high Linear Energy Transfer (LET) radiation was investigated. MATERIALS AND METHODS:High LET iron-ions (500 MeV/n, LET 200 keV/μm) were delivered to G1-phase synchronized Chinese Hamster Ovary (CHO) cells located a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.767990
更新日期:2013-06-01 00:00:00
abstract::The effect of gamma-radiation on aqueous solutions of saturated phospholipids, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG), 1-palmitoyl-2-lyso-sn-glycero-3-phosphocholine (lysoPC), and bovine brain sphingomyelin (SM) has been investigated. It is shown that th...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143888
更新日期:1997-05-01 00:00:00
abstract:PURPOSE:The aim of this research was to demonstrate the effects of 50 Hz sinusoidal extremely low frequency of magnetic fields (ELF-MF) on preincubated chicken embryo hearts at histopathological, biochemical, and ultrastructural levels. MATERIALS AND METHODS:Ninety healthy fertilized eggs were divided into six groups ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.754556
更新日期:2013-04-01 00:00:00
abstract:PURPOSE:To examine the heat sensitivity of DNA-dependent protein kinase (DNA-PK) activity in a variety of cultured mouse, hamster and human cell lines. MATERIALS AND METHODS:Eight cell lines, which have been routinely used in our laboratory, were examined. Cells were heated at 44.0 +/- 0.05 degrees C and DNA-PK activi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000310001596959
更新日期:2003-08-01 00:00:00
abstract::The aim was to identify the decorporation and anti-oxidation efficacy of prompt and delayed consecutive administration of the catecholicpolyaminopolycarboxylate ligands 9501 and 7601 for radiothorium in vivo. The chelating agents 9501 or 7601 were administered intramuscularly to ICR mice 3 min or 3 days after intraper...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000500141124
更新日期:2005-04-01 00:00:00
abstract::Pre-implantation embryos of the mouse were studied for the occurrence of an adaptive response, i.e. induction of radio-resistance by a previous low dose. Various experimental designs were checked (initial doses between 3 and 10 cGy; second dose 2-6 Gy at 6-24 h after the first dose). Some of the experiments were carri...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214551981
更新日期:1992-08-01 00:00:00