Abstract：
:The radiation response of 15 mammalian cell lines comprising 11 human tumour, two human fibroblast and two murine lymphoma cell lines, has been analysed using the linear-quadratic equation. As well as using conventional analysis of acute dose-survival curves to derive values for alpha and beta (termed alpha ac and beta ac), low dose-rate and split-dose experiments have been used to derive independent values of alpha and beta (alpha 1dr and beta RR), respectively. alpha 1dr provides a measure of irrecoverable damage, the magnitude of which agreed well with the initial slope of the acute survival curve for most cell lines. beta RR derived from split-dose experiments represents a unique measure of recovery for each cell line. Large differences were found between individual values of beta ac and beta RR, especially in the radiosensitive cell lines. Since beta RR is a functional measure of recovery we suggest that this is the more relevant parameter in studies of dose sparing. The most striking result of this analysis was found in considering the alpha/beta ratios. No relationship was observed between alpha ac and beta ac resulting in values of alpha ac/beta ac ranging from 1 to 175. In contrast a positive correlation was observed between alpha 1dr beta RR in the 11 tumour cell lines, giving an alpha/beta ratio of 9.4 +/- 1.8 Gy. This observation of the relative constancy of the ratio for human tumour cells leads to an hypothesis about the role of initial damage as a determinant of radiosensitivity.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Peacock JH,Eady JJ,Edwards SM,McMillan TJ,Steel GGdoi
10.1080/09553009214551241subject
Has Abstractpub_date
1992-04-01 00:00:00pages
479-87issue
4eissn
0955-3002issn
1362-3095pii
H2G6L7MHXUX4MULFjournal_volume
61pub_type
杂志文章abstract：:Radiation survival curves have been obtained for crypts in the small and large intestine of male BDF1 mice using the microcolony assay. Four regions of the small intestine and three regions of the large intestine were studied. One of the major objectives was to determine the optimal conditions for the microcolony assa...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097144265
更新日期：1997-02-01 00:00:00
abstract：PURPOSE:Radiotherapy of cancer carries a perceived risk of inducing secondary cancer and other damage due to dose delivered to normal tissue. While expectedly small, this risk must be carefully analysed for all modalities. Especially in the use of exotic particles like pions and antiprotons, which annihilate and produc...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553000903242081
更新日期：2009-12-01 00:00:00
abstract：PURPOSE:To carry out a comparative study on potential cytogenetic fingerprints for radiation LET in human metaphase lymphocytes. MATERIALS AND METHOD:Human lymphocytes were irradiated in vitro with 3.0 Gy 60Co gamma-rays, 0.9 Gy 3H beta-rays or 0.2 Gy 2.7 Mev neutrons. Detailed chromosome aberrations were analysed by ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050201073
更新日期：2000-12-01 00:00:00
abstract：PURPOSE:To investigate whether extremely low frequency electromagnetic field (ELF-EMF) exposure could induce oxidative stress in workers performing tour-inspection near transformers and distribution power lines. MATERIALS AND METHODS:Occupational short-term 'spot' measurements were performed. In total, 310 inspection ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2015.1012304
更新日期：2015-05-01 00:00:00
abstract：PURPOSE:To evaluate a transcriptomic approach to identify healthy women at increased risk of breast cancer due to G2-radiosensitivity and look at transcripts that are differentially expressed between individuals. MATERIALS AND METHODS:We perform the first study to assess the association of G2 radiosensitivity with bas...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000701317366
更新日期：2007-06-01 00:00:00
abstract：PURPOSE:Cells derived from individuals in which the ataxia telangiectasia (ATM) gene is mutated are hypersensitive to ionizing radiation. Whether differences in ATM protein levels exist among human malignant glioma cell lines and whether such differences are correlated with cellular radiosensitivity were determined. M...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098141591
更新日期：1998-08-01 00:00:00
abstract：PURPOSE:A model that identifies radiation-induced genetic instability as the earliest cellular event in the multi-step sequence leading to radiation-induced cancer was previously proposed. In this paper ongoing experiments are discussed which are designed to test this model and its predictions in mouse mammary epitheli...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/095530098141023
更新日期：1998-12-01 00:00:00
abstract：:Existing models of chromosome aberrations produced by ionizing-radiation predict equal numbers of dicentrics and translocations if the dose is so low that complex aberrations can be ignored. We show that, for a specific subset of aberrations detected by FISH, dicentric/translocation equality is predicted even at highe...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145977
更新日期：1996-02-01 00:00:00
abstract：:Ionizing radiation induces both quantitative and qualitative changes in the lymphoid cells of both man and experimental animals, including inhibition of antibody responses. However, the cellular basis of this immunological lesion is not clear. In the present study, groups of mice were exposed to 2.0 Gy gamma-rays or s...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514550531
更新日期：1995-04-01 00:00:00
abstract：PURPOSE:To study the absorptive function of rat colon following whole-body exposure to neutron irradiation, either to the same total dose with varying proportion of neutrons or to the same neutron proportion with an increasing irradiation dose. MATERIALS AND METHODS:Different proportions of neutron irradiation were pr...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/0955300021000019221
更新日期：2002-12-01 00:00:00
abstract：:The mitogen phytohemagglutinin (PHA) works well in both human and cynomolgus monkey (Macaca fascicularis) lymphocyte cultures to stimulate T cell proliferation. T cells from rhesus monkeys (Macaca mulatta) are less responsive than human cells, producing few metaphases when thousands are required, e.g. in biological do...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414551321
更新日期：1994-10-01 00:00:00
abstract：PURPOSE:The LET position of the RBE maximum and its dependence on the cellular repair capacity was determined for carbon ions. Hamster cell lines of differing repair capacity were irradiated with monoenergetic carbon ions. RBE values for cell inactivation at different survival levels were determined and the differences...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099139232
更新日期：1999-11-01 00:00:00
abstract：PURPOSE:The main aim of the present study is to gain mechanistic insights into the modulating effect of molecular hydrogen on the γ-radiation-induced alteration pathways of DNA nucleobases. MATERIALS AND METHODS:Aerated aqueous solutions of calf thymus DNA were exposed to a (60)Co source at doses ranging from 0 to 55 ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2016.1206234
更新日期：2016-09-01 00:00:00
abstract：PURPOSE:To investigate the potential total and relative free-radical transfer from histones to DNA in X-irradiated chromatin, by analysing the relative contributions of individual radicals from protein and from DNA. MATERIALS AND METHODS:Chromatin was isolated from calf thymus, freeze-dried and either used as such or ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050111541
更新日期：2000-08-01 00:00:00
abstract：:Dose responses for the effects of four calcium antagonists on SCCVII/St back and leg tumour sensitivity to 20 Gy X-rays, and on tumour perfusion indicated by relative uptake of 86rubidium, were determined. The effects of these agents on 86rubidium uptake in some normal tissues were also investigated. Flunarizine sensi...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008914551511
更新日期：1989-09-01 00:00:00
abstract：PURPOSE:Ionizing radiation causes various types of DNA damage e.g. single strand breaks (SSB) and double strand breaks (DSB), whereby the SSB/DSB ratio is shifted toward the DSB with increasing LET. For the DNA-incorporated Auger electron emitter Iodine-125 a SSB/DSB ratio of 5.4:1 is calculated based on computer simul...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1851059
更新日期：2020-12-03 00:00:00
abstract：:In mice, external X- or gamma-irradiation may induce thymic lymphomas or myeloid leukaemias, while bone-seeking alpha-emitters may induce osteosarcomas and, to a lesser extent, acute myeloid leukaemia. The present paper aims to review briefly some of the experimental data with respect to the molecular mechanisms under...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009014550851
更新日期：1990-04-01 00:00:00
abstract：:The effects of UV radiation on humans and animals are receiving increasing attention and much interest has recently been focused on the environmental effects of UV A and UV B. This study compares the in vitro effects of UV A and UV B on the clonogenic survival of two human skin keratinocyte cell lines, HaCaT which are...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097143590
更新日期：1997-07-01 00:00:00
abstract：:In the last decade the 'comet assay' or 'single cell gel electrophoresis assay' has been established as a sensitive method for the detection of DNA damage and the measurement of its recovery. The results published in the literature have often been obtained with different methods for comet structure measurement. In mos...
journal_title：International journal of radiation biology
pub_type：
doi：10.1080/095530097143220
更新日期：1997-10-01 00:00:00
abstract：:During previous studies on the relationship between thermosensitivity and intracellular pH (pHi) regulation in mammalian cells, we observed that the thermoresistant TR-4 cells appeared to be resistant to manipulations of the Na+/H+ antiport, one of the two primary membrane regulators of pHi. We hypothesized that this ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096144969
更新日期：1996-10-01 00:00:00
abstract：PURPOSE:The molecular basis of gene regulation in cells exposed to ionising radiation is not fully understood. Gene regulation occurs at the transcriptional and post-transcriptional levels. Recent studies have suggested that micro-RNA (miRNA) plays a significant role at the post-transcriptional gene regulation. miRNA a...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553001003734568
更新日期：2010-07-01 00:00:00
abstract：PURPOSE:Estimating the absorbed dose to tumour relative to normal tissues has often been used in the assessment of the therapeutic efficacy of radiolabelled antibodies for radioimmunotherapy. Typically, the calculations assume a uniform dose deposition and response throughout the tumour. However, the heterogeneity of t...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110117818
更新日期：2002-05-01 00:00:00
abstract：:Purpose: Ionizing radiation is a risk factor to the whole organism, including the heart. Cardiac damage is considered to be a late effect of radiation exposure. While the acute cardiotoxicity of high doses is well characterized, the knowledge about nature and magnitude of the cardiac risk following lower doses exposur...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2020.1704299
更新日期：2020-03-01 00:00:00
abstract：:This study was designed to compare radiosensitization by the oral platinum compound JM216 with cisplatin. RIF1 mouse tumour cells were treated at various doses and at various exposure times with JM216 and irradiated 15 min before the end of drug exposure. The fraction of cells surviving treatment was assessed by colon...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097142834
更新日期：1997-12-01 00:00:00
abstract：PURPOSE:To assess the potential of using the residual phosphorylation of histone H2AX (gammaH2AX) after irradiation as a marker of radiosensitivity in vitro. MATERIAL AND METHODS:Confluent cell cultures of FaDu and SKX human squamous cell carcinoma lines were irradiated with graded single doses. Twenty-four hours afte...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553000903242149
更新日期：2009-11-01 00:00:00
abstract：PURPOSE:Ionizing radiation induces DNA damage, some of which are present in clusters, defined as two or more lesions within one to two helical turns of DNA by passage of a single radiation track. These clusters are thought to contribute to the detrimental effects of radiation, in part due to the compromised repair of c...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2014.899449
更新日期：2014-06-01 00:00:00
abstract：PURPOSE:In a previous study, we found that exposure to a 50 Hz magnetic field (MF) could activate stress-activated protein kinase (SAPK) and P38 mitogen-activated protein (MAP) kinase (P38 MAPK) in Chinese hamster lung (CHL) fibroblast cells, and simultaneous exposure to a 'noise' MF of the same intensity inhibited the...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000801998875
更新日期：2008-05-01 00:00:00
abstract：PURPOSE:A minority of patients exhibits severe late normal tissue toxicity after radiotherapy (RT), possibly related to their inherent individual radiation sensitivity. This study aimed to evaluate four different candidate in vitro cellular radiosensitivity assays for prediction of late normal tissue reactions, in a re...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2016.1230238
更新日期：2016-12-01 00:00:00
abstract：PURPOSE:The tumour suppressor protein p53 is considered to have an impact on the radiosensitivity of tumour cells. However, this concept does not easily translate to the tumour sensitivity in the clinics. The aim of the present study was to determine whether a functional or dysfunctional p53 is associated with a sensit...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000310001642902
更新日期：2004-01-01 00:00:00
abstract：:Lethal mutations are induced in mammalian cells mainly, perhaps wholly, within the dose range encompassing the 'shoulder,' and may well be attributable to error-prone Q-repair. ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008814551961
更新日期：1988-10-01 00:00:00
