Abstract:
PURPOSE:To develop a procedure, using the inverse polymerase chain reaction, to detect and sequence ionizing radiation-induced DNA rearrangements without prior phenotypic selection of mutant cells. METHOD:Normal human fibroblast cells (IMR-90) were given 30Gy of gamma-irradiation and then incubated at 37 degrees C for 23h to allow DNA repair. Rearrangements of the sequence 5' to the c-myc gene were examined by amplifying the region using inverse PCR followed by DNA sequencing. RESULTS:Approximately fivefold more PCR products were amplified from the DNA of cells given 30 Gy of gamma-irradiation and allowed 23 h for repair than were obtained from cells that were either unirradiated or were irradiated and then lysed immediately. PCR products from seven putative radiation-induced DNA rearrangements were sequenced. Of these products, one contained an unidentified sequence (a possible inter-chromosomal rearrangement) whilst the other products appeared to derive from episomes or duplication events (possible intra-chromosomal rearrangements). The sequencing data suggested that the sites of DNA rearrangement breakpoints were non-randomly distributed and possibly associated with topoisomerase I consensus cleavage sequences. There was a significant level of direct homology between the sequences flanking the breakpoints. CONCLUSIONS:The procedure developed was able to detect both inter- and intra-chromosomal rearrangements.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Forrester HB,Radford IRdoi
10.1080/095530098141672subject
Has Abstractpub_date
1998-07-01 00:00:00pages
1-15issue
1eissn
0955-3002issn
1362-3095journal_volume
74pub_type
杂志文章abstract:PURPOSE:To calculate survival of human haemopoietic stem cells irradiated by alpha particles from 149Tb (initial energy 3.97 MeV) and 211At (average initial energy 6.87 MeV). METHODS:Following as closely as possible radiobiological data, Monte Carlo methods were used to calculate passages of alpha particles (originati...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141771
更新日期:1998-07-01 00:00:00
abstract::Radiation-induced DNA double-strand breaks (dsb) were studied in Ehrlich ascites tumour cells (EATC) by sedimentation in neutral sucrose gradients at low centrifuge speed. Dsb induction was found to be linear with dose with a frequency of: ndsbmr-1D-1 = (11.7 +/- 2) x 10(-12)Gy-1 for 140 kV X-rays and ndsbmr-1D-1 = (1...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814552201
更新日期:1988-11-01 00:00:00
abstract::Intact Chinese hamster ovary cells were exposed to the restriction endonucleases (REs) AluI or BamHI. In metaphase spreads from these cells, 300 breakpoints per RE were localized in G-banded chromosome type aberrations (dicentrics, translocations, rings, terminal and interstitial deletions). The majority of breakpoint...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514551401
更新日期:1995-10-01 00:00:00
abstract:PURPOSE:It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2018.1518611
更新日期:2018-11-01 00:00:00
abstract:PURPOSE:We computed probabilities to have multiple double-strand breaks (DSB), which are produced in DNA on a regional scale, and not in close vicinity, in volumes matching the size of DNA damage foci, of a large chromatin loop, and in the physical volume of DNA containing the HPRT (human hypoxanthine phosphoribosyltra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802499212
更新日期:2008-11-01 00:00:00
abstract:PURPOSE:To study the oxidative stress-induced changes by sublethal doses of cadmium chloride (CdCl2) and gamma irradiation, two redox-inducing agents, on metallothionein (MT) gene and protein expression in Plantago ovata Forsk (P. ovata). MATERIALS AND METHODS:Chlorophyll content was estimated to study the stress resp...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.782109
更新日期:2013-07-01 00:00:00
abstract:PURPOSE:To examine whether a simulated mobile telephone transmission at 915 MHz has an effect on cognitive function in man. MATERIALS AND METHODS:Thirty-six subjects in two groups were each given two training sessions and then three test sessions in a randomized three-way cross-over design. About 1 W mean power at 915...
journal_title:International journal of radiation biology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1080/095530099140375
更新日期:1999-04-01 00:00:00
abstract:PURPOSE:To determine whether murine lymphoid cell lines can discriminate between high- and low-LET (linear energy transfer) radiation-induced DNA lesions. MATERIALS AND METHODS:Sensitivity to killing by DNA-incorporated 3H and 125I decays, accumulated during storage in the gas phase of a liquid nitrogen tank, was dete...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110120337
更新日期:2002-06-01 00:00:00
abstract:PURPOSE:One of the most important issues regarding radiofrequency electromagnetic fields (RF-EMF) is their effect on genetic material. Therefore, we investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields (RF-EMF) and the effect of a recovery period after exposure to RF-EMF on bone marro...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.809170
更新日期:2013-11-01 00:00:00
abstract:PURPOSE:The tandemly repeated hexamers (TTAGGG)n present at the telomeric ends protect the human genome from a variety of environmental exposures including ionizing radiation. In order to find out the effect of chronic low dose radiation exposure, we have determined telomere length among newborns from high level natura...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.699135
更新日期:2012-09-01 00:00:00
abstract:PURPOSE:The behavioral responses of planaria to the exposures of a range of concentrations of morphine (μM to attoM) or the μ-opiate antagonist naloxone or to either of these compounds and a burst-firing magnetic field (5 μT) were studied. MATERIAL AND METHODS:The locomotor velocity (LMV) of planaria was measured afte...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.911421
更新日期:2014-10-01 00:00:00
abstract::The effect of heat (43-45 degrees C) on the induction and the repair of DNA double-strand breaks (dsb) was studied in CHO cells after 60 Gy of X-rays using constant-field gel electrophoresis. Heat given prior to irradiation was found not to alter the number of dsb when measured immediately after irradiation. In non-he...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143392
更新日期:1997-08-01 00:00:00
abstract::Cultures of JU-56 cells were irradiated in either G1 or G2 and examined either in their first post-irradiation metaphase (in diploids) or in their second post-irradiation metaphase (in colcemid-induced tetraploids). The timing of fixation, together with tritiated thymidine pulse labelling, allowed selection for scorin...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314552131
更新日期:1993-06-01 00:00:00
abstract::Electron transfer to 5-bromouracil (5-BrU) from nucleobase (N) electron adducts (and their protonated forms) has been studied by product analysis and pulse radiolysis. When an electron is transferred to 5-BrU, the ensuing 5-BrU radical anion rapidly loses a bromide ion; the uracilyl radical thus formed reacts with add...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214552451
更新日期:1992-11-01 00:00:00
abstract:PURPOSE:To investigate the p53 ionizing radiation-induced response, G1/S cell-cycle block and cytogenetic damage in Nijmegen breakage syndrome (NBS) cells characterized by different haplotypes. MATERIAL AND METHODS:Lymphoblastoid cell lines derived from three normals, five NBS and two ataxia telangiectasia (AT) indivi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099140221
更新日期:1999-05-01 00:00:00
abstract::Induction of mutations to 6-thioguanine resistance (TGr) by gamma-rays at three different dose-rates and molecular changes in the HPRT gene were studied in human lymphoblastoid WIL2-NS cells. Mutant induction showed a curvilinear dose-response for acute irradiation (30 Gy/h). The total mutant frequency was lower after...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145201
更新日期:1996-08-01 00:00:00
abstract:PURPOSE:The deleterious processes triggered by heavy ions on DNA were studied through the determination of the yield of a series of oxidized bases. Emphasis was placed on the estimation of the respective contribution of direct ionization and indirect effects, mostly by comparison with low linear energy transfer (LET) g...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000600573788
更新日期:2006-02-01 00:00:00
abstract:PURPOSE:To investigate the influence of chromatin organization and scavenging capacity in relation to irradiation temperature on the induction of double-strand breaks (DSB) in structures derived from human diploid fibroblasts. MATERIALS AND METHODS:Agarose plugs with different chromatin structures (intact cells+/-wort...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000310001610204
更新日期:2003-10-01 00:00:00
abstract::Calibration of the non-denaturing (pH 9.6) filter elution technique by 125I decays has been used to examine the relationship between X-ray-induced DNA double-strand breaks (dsb) and cellular survival of CHO cells and human fibroblasts. DNA dsb induction varies markedly between the two cell lines, reflecting the differ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314550411
更新日期:1993-03-01 00:00:00
abstract::Cell cultures derived from skin biopsies of mouse fetuses X-irradiated at the zygote stage (2 Gy) were studied for the presence of chromosomal aberrations and micronuclei. In comparison with cells from control, unirradiated fetuses significantly higher spontaneous frequencies of aberrations per 100 metaphases and aber...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914550091
更新日期:1989-01-01 00:00:00
abstract::Terminally differentiated cells usually do not divide and are, thus, reproductively dead. To elucidate the significance of radiation-enhanced differentiation to reproductive cell death, murine erythroid progenitor cells were gamma-irradiated in plasma clot cultures and the development of haemoglobinized clones was stu...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145869
更新日期:1996-03-01 00:00:00
abstract::The aim was to investigate further the relationship between radiation-induced mitotic delay and the expression of chromosome damage in V79 cells. Recently published data on the time-course of chromosome aberrations in V79 first-cycle metaphases after exposure to 10.4 MeV u(-1) Ar ions (LET = 1226 keV microm(-1)) were ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000400027902
更新日期:2005-01-01 00:00:00
abstract::Tikvah Alper's interest in science was broad, from scrapie to mammalian cells and cancer. Much of her own work focused on cell lethality, like that of many other radiobiologists, but this was natural because of the simplicity of the endpoint cell survival and its relevance to cancer therapy. Tikvah had broader interes...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143662
更新日期:1997-06-01 00:00:00
abstract:PURPOSE:To determine whether cigarette smoke condensate (CSC) without metabolic activation induces direct DNA double strand breaks (DSB) in the G1 phase of various radiosensitive mutants of CHO cells and whether these breaks display collateral hypersensitivity to CSC with respect to cell killing. MATERIALS & METHODS:W...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000701481790
更新日期:2007-09-01 00:00:00
abstract:PURPOSE:Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments describ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138844
更新日期:2000-02-01 00:00:00
abstract::We studied the extent of split-dose recovery in seven non-transformed human fibroblast cell lines of different intrinsic radiosensitivity (HF19, 1BR3, 149BR, 84BR, GM739, 180BR and AT2EM). Experiments were performed on both growing and plateau-phase cells. The seven cell lines displayed a wide range of intrinsic radio...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096146075
更新日期:1996-02-01 00:00:00
abstract:PURPOSE:5-Formyluracil (5-foU) is a major derivative of thymine produced in DNA by ionizing radiation and various chemical oxidants. It has been previously shown that 5-foU in template DNA directs misincorporation of nucleotides by DNA polymerases during in vitro DNA synthesis. The present experiments were designed to ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530001453113
更新日期:2001-01-01 00:00:00
abstract::The repair activity of verbascoside (VER), isolated from Pedicularis spicata, towards the oxidizing hydroxyl radical adduct of dGMP and its reaction mechanism were studied using pulse radiolysis. Upon pulse radiolysis of nitrous oxide saturated aqueous solution of 2'-deoxyguanosine-5'-monophosphate (dGMP) and VER, it ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145779
更新日期:1996-04-01 00:00:00
abstract::The kinetic scheme of the reaction of desferrioxamine (DFO) with O2-. was studied using pulse and gamma-radiolysis. The rate constant k(O2-. + DFO) is equal to 1.3 +/- 0.1 x 10(6) dm3 mol-1s-1 at pH 7.4. Studying the competition between DFO and ferricytochrome-c for O2-. generated by gamma-radiolysis, we observed that...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914552381
更新日期:1989-12-01 00:00:00
abstract:PURPOSE:To investigate the induction of chromosomal aberrations in mouse m5S cells after exposure to power-line frequency magnetic fields (extremely low frequency magnetic fields; ELFMF) at high-flux densities. MATERIAL AND METHOD:m5S cells were either untreated or pretreated during the G1 phase with mitomycin C (MMC,...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050201172
更新日期:2000-12-01 00:00:00