Abstract：
:The effect of heat (43-45 degrees C) on the induction and the repair of DNA double-strand breaks (dsb) was studied in CHO cells after 60 Gy of X-rays using constant-field gel electrophoresis. Heat given prior to irradiation was found not to alter the number of dsb when measured immediately after irradiation. In non-heated cells, about 80% of all dsb were rapidly repaired with a half-time of 4 min, while 20% were repaired more slowly with T4 = 160 min. These kinetics were grossly altered by heat. Both the fast and the slow process were retarded. However, the main effect of heat was an increase in the number of slowly rejoined dsb. This increase was shown to result from the additional formation (up to 1.4-fold the initial number) of dsb early during the repair course. It is suggested that the additional dsb arose from base damage, the repair of which was unbalanced by heat. No evidence was found for apoptosis being involved in this process. The kinetics of the additional dsb was found to correlate with thermal radiosensitization.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Dahm-Daphi J,Brammer I,Dikomey Edoi
10.1080/095530097143392subject
Has Abstractpub_date
1997-08-01 00:00:00pages
171-9issue
2eissn
0955-3002issn
1362-3095journal_volume
72pub_type
杂志文章abstract：:PACE (programmable, autonomously controlled electrodes) pulsed field gel electrophoresis technology was used to develop a DNA double-strand break assay that simultaneously combined (1) resolution across a broad range of DNA sizes, (2) high sensitivity (in terms of detecting dsb at low doses) and (3) speed. A 48 h PACE...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314550021
更新日期：1993-01-01 00:00:00
abstract：:The relative efficiencies of two types of densely ionizing particles were compared for the induction of mutations at two distinct genetic loci in human cells. Mutations to 6-thioguanine resistance (hgprt locus) or to trifluorothymidine resistance (tk) locus were scored in TK6 human lymphoblastoid cells exposed to grad...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008914550961
更新日期：1989-06-01 00:00:00
abstract：:We used a cell sorter assay to evaluate the efficacy of the hypoxic cell sensitizer etanidazole over 3-4 logs of cell inactivation, with particular attention to the clinically relevant low-dose survival region. Analysis of the radiation responses in a panel of six human tumour cell lines under conditions of hypoxia an...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096144617
更新日期：1996-12-01 00:00:00
abstract：PURPOSE:To assess the possibility that extremely low frequency (ELF) magnetic fields obstruct the damage repair process, the gene conversion frequency and cell cycle kinetics in a DNA repair-proficient and nucleotide excision repair (NER)-deficient strain of diploid yeast Saccharomyces cerevisiae. MATERIALS AND METHOD...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000310001621437
更新日期：2003-11-01 00:00:00
abstract：PURPOSE:To investigate whether an adaptive response can be induced in mice which were pre-exposed to 900 MHz radiofrequency fields. MATERIALS AND METHODS:Adult male Kunming mice were exposed to 900 MHz radiofrequency fields (RF) at power intensities of 12, 120 and 1200 μW/cm(2) for 1 h/day for 14 days and then subject...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.550981
更新日期：2011-07-01 00:00:00
abstract：PURPOSE:The aim of this work is to study the relative damaging effects of DNA-incorporated radionuclides by analyzing the behavior of the liquid-scintillation counting efficiency in volumes of nanometer size. METHODS:A liquid scintillation counter can detect changes in the micelle size when different percentages of an...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000802460156
更新日期：2008-12-01 00:00:00
abstract：BACKGROUND:Element-specific biokinetic models are used to reconstruct doses to systemic tissues from internal emitters. Typically, a systemic model for a radionuclide explicitly depicts only its dominant repositories. Remaining tissues and fluids are aggregated into a pool called Other tissue in which the radionuclide ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2018.1554923
更新日期：2018-12-04 00:00:00
abstract：:The radiation response of 15 mammalian cell lines comprising 11 human tumour, two human fibroblast and two murine lymphoma cell lines, has been analysed using the linear-quadratic equation. As well as using conventional analysis of acute dose-survival curves to derive values for alpha and beta (termed alpha ac and bet...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214551241
更新日期：1992-04-01 00:00:00
abstract：:Human ovarian carcinoma parental and cisplatin-resistant cells were evaluated for their radiation sensitivity to high and low dose-rate irradiation and for the effectiveness of cisplatin in radiosensitization. The cisplatin resistant variant A2780cp showed increased radiation resistance for both low dose-rate (LDRI) a...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145634
更新日期：1996-05-01 00:00:00
abstract：:Base damages, sugar damages, and single-strand breaks produced by free radicals are the preponderant lesions produced in DNA by ionizing radiation. These lesions have been individually introduced into substrate, template, and biologically active DNA molecules and enzymatic processing and biological consequences determ...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009414551661
更新日期：1994-11-01 00:00:00
abstract：PURPOSE:The aim is to assess the change with age and disease of the oxygen concentration within the coronary artery walls. MATERIALS AND METHODS:In an accompanying paper, Part A, the age-dependent morphology and composition for the wall layers of normal and diseased coronary arteries were developed from published data...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000802389645
更新日期：2008-10-01 00:00:00
abstract：PURPOSE:The actinides are chemical poisons and radiological hazards. One challenge to better appraise their toxicity and develop countermeasures in case of exposure of living organisms is to better assess pathways of contamination. Because of the high chemical affinity of those actinide elements for phosphate groups an...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2014.886795
更新日期：2014-11-01 00:00:00
abstract：:We have re-examined the effect of detergent type, pH and temperature of lysis on the shape of the DNA elution dose response under non-denaturing conditions using plateau-phase CHO cells. Results practically identical to those previously published (Okayasu and Iliakis, 1989) were obtained, with a 1 h incubation at 60 d...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214551211
更新日期：1992-04-01 00:00:00
abstract：PURPOSE:Radiotherapy is an important treatment option for brain tumors, but the unavoidable irradiation of normal brain tissue can lead to delayed cognitive impairment. The mechanisms involved are still not well explained and, therefore, new tools to investigate the processes leading to the delayed symptoms of brain ir...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1787542
更新日期：2020-09-01 00:00:00
abstract：:Purpose To investigate the effect of increasing the overall treatment time as well as delivering the compensating doses on the Balb/c breast adenocarcinoma (4T1) tumor. Materials and methods A total of 72 mice were divided into two aliquots (classes A and B) based on the initial size of their induced tumor. Each class...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2016.1117677
更新日期：2016-01-01 00:00:00
abstract：:The yields of strand break formation (Gssb) in single-stranded DNA (ssDNA) initiated by radiation-generated OH radicals have been determined using the method of low-angle laser light scattering (LALLS). The irradiations were carried out in aqueous, N2O-saturated solutions in the absence and presence of oxygen and at d...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014551951
更新日期：1990-10-01 00:00:00
abstract：PURPOSE:The assessment of biological effects caused by radiation exposure has been currently carried out with the linear-quadratic (LQ) model as an extension of the linear non-threshold (LNT) model. In this study, we suggest a new mathematical model named as SeaSaw (SS) model, which describes proliferation and cell dea...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2021.1854487
更新日期：2020-12-15 00:00:00
abstract：PURPOSE:To develop and validate non-fluorescent chromosome painting for bright-field microscopy using the peroxidase/diaminobenzidine (DAB) reaction. MATERIALS AND METHODS:Peripheral blood lymphocytes were taken from patients with uterine cancer who had received heavy-ion radiation therapy. Chromosome slides were trea...
journal_title：International journal of radiation biology
pub_type：
doi：10.1080/095530098142077
更新日期：1998-05-01 00:00:00
abstract：:The role of protein kinase C in radiation-induced death of thymocytes was studied. For this purpose murine thymocytes were irradiated and incubated for 6 h at 37 degrees C and afterwards the fraction of fragmented DNA was measured. Results indicate that radiation-induced DNA fragmentation can be prevented by adding th...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214551471
更新日期：1992-05-01 00:00:00
abstract：PURPOSE:Caveolin-1 is a membrane protein highly expressed in many tumors and plays an important role in tumor progression and metastasis. This review describes the structure of the Caveolin-1 protein and its pre-clinical and clinical significance, demonstrating that Caveolin-1 is a novel biomarker for radioresistance w...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2016.1222096
更新日期：2016-12-01 00:00:00
abstract：:The relative contributions of the OH-mediated and direct radiation effect on the induction of DNA double-strand breaks (dsbs) were evaluated in two haploid yeast cell lines (GSH+ and gsh-) irradiated under oxic or hypoxic conditions in the absence or presence of 6 M glycerol as an OH radical scavenger. Gsh- cells are ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551721
更新日期：1993-11-01 00:00:00
abstract：PURPOSE:The molecular basis of gene regulation in cells exposed to ionising radiation is not fully understood. Gene regulation occurs at the transcriptional and post-transcriptional levels. Recent studies have suggested that micro-RNA (miRNA) plays a significant role at the post-transcriptional gene regulation. miRNA a...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553001003734568
更新日期：2010-07-01 00:00:00
abstract：:Calibration of the non-denaturing (pH 9.6) filter elution technique by 125I decays has been used to examine the relationship between X-ray-induced DNA double-strand breaks (dsb) and cellular survival of CHO cells and human fibroblasts. DNA dsb induction varies markedly between the two cell lines, reflecting the differ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314550411
更新日期：1993-03-01 00:00:00
abstract：:Ionizing radiation induces both quantitative and qualitative changes in the lymphoid cells of both man and experimental animals, including inhibition of antibody responses. However, the cellular basis of this immunological lesion is not clear. In the present study, groups of mice were exposed to 2.0 Gy gamma-rays or s...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514550531
更新日期：1995-04-01 00:00:00
abstract：PURPOSE:To carry out a comparative study on potential cytogenetic fingerprints for radiation LET in human metaphase lymphocytes. MATERIALS AND METHOD:Human lymphocytes were irradiated in vitro with 3.0 Gy 60Co gamma-rays, 0.9 Gy 3H beta-rays or 0.2 Gy 2.7 Mev neutrons. Detailed chromosome aberrations were analysed by ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050201073
更新日期：2000-12-01 00:00:00
abstract：:A study has been made of the intrinsic radiosensitivity of peripheral blood lymphocytes from normal individuals. Cell survival following in vitro high (1.55 Gy min-1) and low (0.0098 Gy min-1) dose-rate irradiation was obtained for single lymphocyte samples from three individuals in six experiments. Despite wide inter...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551571
更新日期：1993-10-01 00:00:00
abstract：PURPOSE:The tumour suppressor protein p53 is considered to have an impact on the radiosensitivity of tumour cells. However, this concept does not easily translate to the tumour sensitivity in the clinics. The aim of the present study was to determine whether a functional or dysfunctional p53 is associated with a sensit...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000310001642902
更新日期：2004-01-01 00:00:00
abstract：PURPOSE:To compare the chromosomal radiosensitivity of C3H mouse acute myeloid leukaemia (AML) cell lines 7926 and 8709 and to investigate the mechanistic basis of the radiosensitivity observed in 7926. MATERIALS AND METHODS:Yields of chromosome aberrations following X-irradiation were determined in Giemsa-stained met...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110075220
更新日期：2001-12-01 00:00:00
abstract：PURPOSE:Ionizing radiation causes various types of DNA damage e.g. single strand breaks (SSB) and double strand breaks (DSB), whereby the SSB/DSB ratio is shifted toward the DSB with increasing LET. For the DNA-incorporated Auger electron emitter Iodine-125 a SSB/DSB ratio of 5.4:1 is calculated based on computer simul...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1851059
更新日期：2020-12-03 00:00:00
abstract：:Single crystals of cytosine hydrochloride with thiocytosine as an impurity were found suitable for the study of a possible new mechanism of long-range migration of energy deposited by ionizing radiation. In a crystal containing thiocytosine, two kinds of chlorine-containing paramagnetic centres are present that are co...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414550891
更新日期：1994-07-01 00:00:00
