Abstract：
:A comparison has been made of the in vitro light-activated drug cytotoxicities of two different porphyrin compounds, Photofrin II and TPPS4. An early passage human colon adenocarcinoma cell line, WiDr, has been exposed to either drug for 24 h, the excess drug washed from the cells and the cells irradiated with light using quartz-tungsten-halogen lamps. Neither light nor drug alone under the experimental conditions employed was toxic to WiDr cells. Together, considerable cytotoxicity could be seen and the shapes of the cell survival curves following exposure to either drug then irradiation with light, were similar. For equal amounts of drug in the medium, Photofrin II was a more efficient photosensitizer of WiDr cells than TPPS4, and differences in cellular uptake could only partly explain this. When the experimental procedure was changed by reducing the temperature of irradiation, a reduction in photosensitizing efficiency could be demonstrated. This was more pronounced for Photofrin II, and was seen as a change in the slope of the final portion of the survival curve; and as a change in the shoulder for TPPS4. Two different batches of the two drugs were compared and shown to give slightly different results for Photofrin II (change in shoulder) but not to differ for TPPS4.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
West CM,Moore JVdoi
10.1080/09553008814552061subject
Has Abstractpub_date
1988-10-01 00:00:00pages
621-34issue
4eissn
0955-3002issn
1362-3095pii
FF5G7JP8628Q36YGjournal_volume
54pub_type
杂志文章abstract：:Base damages, sugar damages, and single-strand breaks produced by free radicals are the preponderant lesions produced in DNA by ionizing radiation. These lesions have been individually introduced into substrate, template, and biologically active DNA molecules and enzymatic processing and biological consequences determ...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009414551661
更新日期：1994-11-01 00:00:00
abstract：PURPOSE:To study cell damage and possible apoptosis in K562 human erythroleukemic cells exposed for 2 h to an extremely low frequency (ELF) 50 Hz sinusoidal magnetic field with a magnetic induction of either 1 or 5 mT using high resolution proton nuclear magnetic resonance (1H-NMR) spectroscopy. MATERIALS AND METHODS:...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000500102985
更新日期：2005-02-01 00:00:00
abstract：PURPOSE:This study evaluates early changes in human mesenchymal stem cells (MSC) isolated from dental pulp and periodontal ligament after γ-irradiation and the effect of ataxia-telangiectasia mutated (ATM) inhibition. METHODS:MSC were irradiated with 2 and 20 Gy by (60)Co. For ATM inhibition, specific inhibitor KU5593...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.775530
更新日期：2013-07-01 00:00:00
abstract：:The recently released data on cancer incidence in the Japanese atomic bomb survivors are analysed using a variety of relative risk models which take account of errors in estimates of dose to assess the dose-response at low doses. For all solid cancers analysed together there is a significant positive dose-response (at...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145364
更新日期：1996-07-01 00:00:00
abstract：PURPOSE:Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments describ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138844
更新日期：2000-02-01 00:00:00
abstract：:The accumulation and repair of radiation-induced DNA double-strand breaks (dsbs) were determined by neutral filter elution on 20 primary cultures obtained from ovarian cancer and malignant melanoma clinical specimens. The initial frequency of DNA dsbs after exposure to 50 Gy gamma-irradiation varied greatly for the in...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414551211
更新日期：1994-09-01 00:00:00
abstract：PURPOSE:This review examines the evidence for the hypothesis that epigenetics are involved in the initiation and perpetuation of radiation-induced genomic instability (RIGI). CONCLUSION:In addition to the extensively studied targeted effects of radiation, it is now apparent that non-targeted delayed effects such as RI...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.3109/09553002.2010.522686
更新日期：2011-02-01 00:00:00
abstract：PURPOSE:The radiation-induced bystander effect (RIBE) has important implications for the efficiency of radiotherapy but the underlying role of cellular metabolism is widely unknown. The roles of synthesis of cytochrome c oxidase 2 (SCO2), a key effector for respiratory chain, and related signaling factors in α-particle...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.817706
更新日期：2013-12-01 00:00:00
abstract：:Despite the extensive utilization of radiotherapy in cancer treatments, clinicians often face challenges in achieving desired outcomes. This is because of the existence of secondary radiation effects like bystander phenomenon that influence radiation nontargeted cells and tissues. The concept of bystander effects of r...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2019.1547440
更新日期：2019-03-01 00:00:00
abstract：PURPOSE:To investigate cell cycle effects and relative biological effectiveness (RBE) of α-particles from the clinically relevant radionuclide Astatine-211 ((211)At), using X-rays as reference radiation. Double-strand breaks (DSB), non-DSB clusters containing oxidised purines and clonogenic survival were investigated. ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2011.538127
更新日期：2011-04-01 00:00:00
abstract：PURPOSE:The long-term effect of low and moderate doses of ionizing radiation on the lens is still a matter of debate and needs to be evaluated in more detail. MATERIAL AND METHODS:We conducted a detailed histological analysis of eyes from B6C3F1 mice cohorts after acute gamma irradiation (60Co source; 0.063 Gy/min) at...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2021.1876951
更新日期：2021-01-19 00:00:00
abstract：PURPOSE:In a previous study, we found that exposure to a 50 Hz magnetic field (MF) could activate stress-activated protein kinase (SAPK) and P38 mitogen-activated protein (MAP) kinase (P38 MAPK) in Chinese hamster lung (CHL) fibroblast cells, and simultaneous exposure to a 'noise' MF of the same intensity inhibited the...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000801998875
更新日期：2008-05-01 00:00:00
abstract：:To characterize further the radiosensitivity of severe combined immunodeficiency (scid) mice, the induction of micronuclei (MN) in polychromatic erythrocytes as well as cell killing and translocation induction in stem cell spermatogonia was studied. Scid mice turned out to be clearly hypersensitive for X-ray-induced c...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514550661
更新日期：1995-05-01 00:00:00
abstract：:Only a small percentage of the DNA damage set by ionizing radiation in the living cell manifests itself as lethal. It is now increasingly accepted that clustered lesions may constitute the kind of damage that the repair enzymes cannot adequately deal with. The question is raised as to whether damage amplification reac...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414551501
更新日期：1994-11-01 00:00:00
abstract：PURPOSE:The nature of the transferrable factor which goes from irradiated objects to bystander objects remains undefined. Most agree that a chemical entity is the likely 'factor' although some authors have produced in vitro evidence for the involvement of a physical component or a very potent volatile capable of travel...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2012.698366
更新日期：2012-08-01 00:00:00
abstract：PURPOSE:To determine the relative toxicity of alpha- and beta-radiations under conditions of controlled temporal and spatial dose distribution. METHODS:Fused aluminosilicate particles were radiolabelled with either (45)Ca (a beta-emitter) or (242)Cm (an alpha-emitter). These were injected into CBA/Ca mice to give life...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553000903564075
更新日期：2010-04-01 00:00:00
abstract：:The past recommendations of the ICRP about dose limits to the skin are reviewed. Recently, an ICRP Task Group has been revisiting the old arguments and setting them against new data. With the exception of the function of cells in the skin associated with immunocompetence, non-stochastic effects have been well characte...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009014550961
更新日期：1990-04-01 00:00:00
abstract：:We studied the extent of split-dose recovery in seven non-transformed human fibroblast cell lines of different intrinsic radiosensitivity (HF19, 1BR3, 149BR, 84BR, GM739, 180BR and AT2EM). Experiments were performed on both growing and plateau-phase cells. The seven cell lines displayed a wide range of intrinsic radio...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096146075
更新日期：1996-02-01 00:00:00
abstract：:BW12C, which was developed as an agent for the treatment of sickle cell anaemia, increases the binding of oxygen to haemoglobin and hence reduces the availability of oxygen to tissues. Due to these changes in oxygen availability BW12C could act as a protector against radiation-induced injury to normal tissues. In this...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550921
更新日期：1991-04-01 00:00:00
abstract：:Chinese hamster ovary cells were exposed to various heat treatments followed by X-irradiation, and the induction and repair of DNA strand breaks was studied using the alkaline unwinding technique. Heat treatments alone were found to cause DNA strand breakage only for temperatures greater than or equal to 43 degrees C,...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214550851
更新日期：1992-02-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1820601
更新日期：2020-10-16 00:00:00
abstract：:We used three common fluorescent probes to measure pericentric inversion frequencies in 2.9 Gy 60Co gamma-irradiated human lymphocytes. For a given chromosome, the first probe is specific to one telomeric region, the second probe is specific to one subcentromeric region and the third probe is specific to the centromer...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097144382
更新日期：1997-01-01 00:00:00
abstract：:Survival of HT29 cells was measured after irradiation with single doses of X-rays (0.05-5 Gy) and neutrons (0.025-1.5 Gy), using a Dynamic Microscopic Imaging Processing Scanner (DMIPS) with which individual cells can be accurately located in tissue culture flasks, their positions recorded, and after an appropriate in...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314450831
更新日期：1993-05-01 00:00:00
abstract：PURPOSE:A model that identifies radiation-induced genetic instability as the earliest cellular event in the multi-step sequence leading to radiation-induced cancer was previously proposed. In this paper ongoing experiments are discussed which are designed to test this model and its predictions in mouse mammary epitheli...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/095530098141023
更新日期：1998-12-01 00:00:00
abstract：:We have measured clonal survival following gamma-irradiation of unstimulated (G0) T-lymphocytes from 35 donors, of 11 T-lymphocyte cell lines, of six lymphoblastoid cell lines, and of nine primary fibroblast strains for which we have G0 T-lymphocyte material from the same donor. Amongst the G0 lymphocytes we have resu...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008814552331
更新日期：1988-12-01 00:00:00
abstract：PURPOSE:The purpose of this study was to assess the effect of a lavage solution containing methoxypolyethylene glycol 4-(3,4-dihydroxyphenethylamino)-4-oxobutanoate (MDO) for whole lung lavage (WLL) in dogs after the inhalation of depleted uranium (DU) dust at a dose of 30 mgUkg-1. MATERIALS AND METHODS:WLL was perfor...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2018.1446228
更新日期：2018-05-01 00:00:00
abstract：PURPOSE:One of the most important issues regarding radiofrequency electromagnetic fields (RF-EMF) is their effect on genetic material. Therefore, we investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields (RF-EMF) and the effect of a recovery period after exposure to RF-EMF on bone marro...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.809170
更新日期：2013-11-01 00:00:00
abstract：:Swiss albino mice (7-8 weeks old) were irradiated with different doses (0-25 Gy) of gamma-radiation at a dose-rate of 0.05 Gy/s. The specific activities of glyoxalase I (GI) and glyoxalase II (GII) were determined in the spleen and liver immediately and on the 3rd and 6th day postirradiation. The results indicate that...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314550301
更新日期：1993-02-01 00:00:00
abstract：PURPOSE:To give a state-of-the-art overview on the promise of three-dimensional (3-D) culture systems for anticancer drug development, with particular emphasis on multicellular tumor spheroids (MCTS). RESULTS AND CONCLUSIONS:Cell-based assays have become an integral component in many stages of routine anti-tumor drug ...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553000701727531
更新日期：2007-11-01 00:00:00
abstract：PURPOSE:To construct a quantitative model of the radiation-induced bystander effect based on diffusion-type spreading of bystander signal communication between the hit and non-hit cells. Cell inactivation and induced oncogenic transformation by broad- and microbeam irradiation systems are considered. MATERIALS AND MET...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
