Abstract：
PURPOSE:This study evaluates early changes in human mesenchymal stem cells (MSC) isolated from dental pulp and periodontal ligament after γ-irradiation and the effect of ataxia-telangiectasia mutated (ATM) inhibition. METHODS:MSC were irradiated with 2 and 20 Gy by (60)Co. For ATM inhibition, specific inhibitor KU55933 was used. DNA damage was measured by Comet assay and γH2AX detection. Cell cycle distribution and proteins responding to DNA damage were analyzed 2-72 h after the irradiation. RESULTS:The irradiation of MSC causes an increase in γH2AX; the phosphorylation was ATM-dependent. Irradiation activates ATM kinase, and the level of p53 protein is increased due to its phosphorylation on serine15. While this phosphorylation of p53 is ATM-dependent in MSC, the increase in p53 was not prevented by ATM inhibition. A similar trend was observed for Chk1 and Chk2. The increase in p21 is greater without ATM inhibition. ATM inhibition also does not fully abrogate the accumulation of irradiated MSC in the G2-phase of the cell-cycle. CONCLUSIONS:In irradiated MSC, double-strand breaks are tagged quickly by γH2AX in an ATM-dependent manner. Although phosphorylations of p53(ser15), Chk1(ser345) and Chk2(thr68) are ATM-dependent, the overall amount of these proteins increases when ATM is inhibited. In both types of MSC, ATM-independent mechanisms for cell-cycle arrest in the G2-phase are triggered.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Cmielova J,Havelek R,Kohlerova R,Soukup T,Bruckova L,Suchanek J,Vavrova J,Mokry J,Rezacova Mdoi
10.3109/09553002.2013.775530subject
Has Abstractpub_date
2013-07-01 00:00:00pages
501-11issue
7eissn
0955-3002issn
1362-3095journal_volume
89pub_type
杂志文章abstract：:The repair activity of verbascoside (VER), isolated from Pedicularis spicata, towards the oxidizing hydroxyl radical adduct of dGMP and its reaction mechanism were studied using pulse radiolysis. Upon pulse radiolysis of nitrous oxide saturated aqueous solution of 2'-deoxyguanosine-5'-monophosphate (dGMP) and VER, it ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145779
更新日期：1996-04-01 00:00:00
abstract：PURPOSE:To determine whether murine lymphoid cell lines can discriminate between high- and low-LET (linear energy transfer) radiation-induced DNA lesions. MATERIALS AND METHODS:Sensitivity to killing by DNA-incorporated 3H and 125I decays, accumulated during storage in the gas phase of a liquid nitrogen tank, was dete...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110120337
更新日期：2002-06-01 00:00:00
abstract：PURPOSE:Therapy with low doses of ionising radiation (X-rays) exerts anti-inflammatory effects. Little is known about whether and how low doses of X-ray treatment modulate the inflammatory phenotype of macrophages, especially the secretion of Interleukin-1beta (IL-1β). MATERIALS AND METHODS:Macrophages were differenti...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2012.689464
更新日期：2012-10-01 00:00:00
abstract：:The dose-rate effect has been the topic of extensive radiobiological studies and has important implications in radiation therapy and in the field of radiation protection. Three examples will be discussed: two in radiation therapy and one in protection. First, continuous low dose-rate interstitial brachytherapy may be ...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009114550531
更新日期：1991-03-01 00:00:00
abstract：PURPOSE:A meta-analysis was conducted to obtain a 'quantitative' estimate of the extent of genetic damage in mammalian somatic cells exposed to non-ionizing radiation emitted from extremely low frequency electro-magnetic fields (ELF-EMF) and to compare with that in unexposed control cells. METHODS:The methods used for...
journal_title：International journal of radiation biology
pub_type： 杂志文章,meta分析
doi：10.1080/09553000902748575
更新日期：2009-03-01 00:00:00
abstract：PURPOSE:To study the absorptive function of rat colon following whole-body exposure to neutron irradiation, either to the same total dose with varying proportion of neutrons or to the same neutron proportion with an increasing irradiation dose. MATERIALS AND METHODS:Different proportions of neutron irradiation were pr...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/0955300021000019221
更新日期：2002-12-01 00:00:00
abstract：PURPOSE:To investigate the p53 ionizing radiation-induced response, G1/S cell-cycle block and cytogenetic damage in Nijmegen breakage syndrome (NBS) cells characterized by different haplotypes. MATERIAL AND METHODS:Lymphoblastoid cell lines derived from three normals, five NBS and two ataxia telangiectasia (AT) indivi...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099140221
更新日期：1999-05-01 00:00:00
abstract：:Radiation-induced DNA double-strand breaks (dsbs) were measured in CHO-K1 cells by means of an experimental approach involving constant-field gel electrophoresis and densitometric scanning of ethidium bromide stained gels. For X-irradiation, an induction efficiency of 36 +/- 5 dsbs (Gy x cell)-1 was determined. With t...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514551051
更新日期：1995-08-01 00:00:00
abstract：PURPOSE:To investigate the action of ultra-low dose beta-radiation (ULDBR) on isolated segments of blood vessels. MATERIALS AND METHODS:We used the pharmacological model of isolated rabbit carotid arteries with intact or mechanically removed endothelium. Specific vascular responses to beta-irradiation were registered ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000600562229
更新日期：2005-11-01 00:00:00
abstract：:The relationship between cellular radiosensitivity and normal-tissue response to radiotherapy in individual cancer patients has attracted increasing attention over the last few years. Recent work has suggested that a correlation exists between fibroblast sensitivity and normal-tissue reactions. We have examined the ra...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145021
更新日期：1996-09-01 00:00:00
abstract：PURPOSE:This review will examine the role of phosphatases in cancer cell signalling and also outline emerging findings regarding the influence of phosphatases on tumor cell survival after ionising radiation. CONCLUSION:The exposure of tumour cells to clinically relevant doses of ionising radiation causes DNA damage an...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.3109/09553000903232827
更新日期：2009-11-01 00:00:00
abstract：PURPOSE:To study by fluorescence in situ hybridization (FISH) the involvement of Chinese hamster chromosomes 3, 4, 8, and 9, and their separate chromosome arms in X-ray-induced aberrations. MATERIALS AND METHODS:Male embryonic primary cells of Chinese hamster were used and metaphases were collected and scored at 20 h ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099139700
更新日期：1999-08-01 00:00:00
abstract：PURPOSE:One of the most important issues regarding radiofrequency electromagnetic fields (RF-EMF) is their effect on genetic material. Therefore, we investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields (RF-EMF) and the effect of a recovery period after exposure to RF-EMF on bone marro...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.809170
更新日期：2013-11-01 00:00:00
abstract：:The radiosensitization of two human tumour in vitro cell lines, HT-1080 and LoVo, has been compared with that of the Chinese hamster cell line V79-379A. Although the two human tumour cell lines were more radiosensitive than the V79 cell line sensitizer, enhancement ratios for misonidazole, pimonidazole and azomycin we...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014552461
更新日期：1990-02-01 00:00:00
abstract：PURPOSE:Ifosfamide as a chemotherapeutic drug is used for the treatment of different cancer types. The purpose of this study is the preparation of 99mTc-ifosfamide complex to be evaluated as a potential candidate for tumor imaging. MATERIALS AND METHODS:The radiolabeling of ifosfamide with technetium-99m was carried o...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1524945
更新日期：2018-12-01 00:00:00
abstract：PURPOSE:Radiotherapy is an important treatment option for brain tumors, but the unavoidable irradiation of normal brain tissue can lead to delayed cognitive impairment. The mechanisms involved are still not well explained and, therefore, new tools to investigate the processes leading to the delayed symptoms of brain ir...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1787542
更新日期：2020-09-01 00:00:00
abstract：PURPOSE:To investigate whether extremely low frequency electromagnetic field (ELF-EMF) exposure could induce oxidative stress in workers performing tour-inspection near transformers and distribution power lines. MATERIALS AND METHODS:Occupational short-term 'spot' measurements were performed. In total, 310 inspection ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2015.1012304
更新日期：2015-05-01 00:00:00
abstract：:The existence of a correlation between experimental probabilities of cell inactivation by charged particles and calculated probabilities for K-vacancy production in heavy atoms (C, N, O, P) of the DNA of cell nuclei is established. Both phenomena display a similar dependence upon the linear energy transfer (LET) of in...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414550601
更新日期：1994-05-01 00:00:00
abstract：:We used the 'comet assay' to compare the amount of radiation-induced DNA damage in three tumour cell lines (MeWo, PECA 4451 and PECA 4197) and the extent of DNA repair in two of these lines (MeWo and PECA 4197). Tumour cells were irradiated with X-rays (0.1-10 Gy), embedded in agarose on slides, lysed with sodium dode...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414550371
更新日期：1994-03-01 00:00:00
abstract：PURPOSE:The low dose (<1 Gy) survival curve of mammalian cells is characterized by a region of hypersensitivity (HRS) followed by increased resistance (IRR). Above 1 Gy, the survival response can be described with a smooth downward-bending curve. Indirect evidence has indicated that the IRR response might reflect an in...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138646
更新日期：2000-03-01 00:00:00
abstract：PURPOSE:The identification of fibroblast-like cells of the marrow stroma by means of alkaline phosphatase (ALP) cytochemistry reveals delicate ALP-positive structures interspersed among haematopoietic cells and arranged in a loosely meshed network. These cells are often referred to as 'reticular' cells and the network ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/0955300031000085740
更新日期：2003-04-01 00:00:00
abstract：PURPOSE:Gene therapy combined with radiation has shown promising potential for the treatment of tumors. This paper aimed to clarify the synergistic effect of radiotherapy combined with the bladder cancer tissue-specific oncolytic adenovirus (Ad-PSCAE-UPII-E1A) on bladder cancer cells and to study the underlying synergy...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2017.1231942
更新日期：2017-02-01 00:00:00
abstract：BACKGROUND:High dose ionizing radiation exposure is associated with myelo-depression leading to pancytopenia and the expected clinical manifestations of acute radiation syndrome (ARS). Herein, we evaluated the efficacy of sargramostim (Leukine®, yeast-derived rhu GM-CSF), with regimens delivered at 48, 72, 96, or 120 h...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1673499
更新日期：2020-09-22 00:00:00
abstract：:Sister chromatid exchanges (SCEs) were analysed in lymphocytes from 12 control persons and 33 Chernobyl clean-up workers. The group of Chernobyl clean-up workers consisted of civilians who were forced to go to Chernobyl to clean up environmental contamination caused by Chernobyl disaster. On average, they received 0.1...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514550791
更新日期：1995-06-01 00:00:00
abstract：:The past recommendations of the ICRP about dose limits to the skin are reviewed. Recently, an ICRP Task Group has been revisiting the old arguments and setting them against new data. With the exception of the function of cells in the skin associated with immunocompetence, non-stochastic effects have been well characte...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009014550961
更新日期：1990-04-01 00:00:00
abstract：PURPOSE:It has been proposed previously that the ratio of complete to incomplete translocations as seen by fluorescence in situ hybridization (FISH), the S value, can be a cytogenetic fingerprint of exposure to radiation of different qualities. Results from a previous study suggested that the S value is approximately 1...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099140799
更新日期：1999-01-01 00:00:00
abstract：PURPOSE:To illustrate how probability-generating functions (PGFs) can be employed to derive a simple probabilistic model for clonogenic survival after exposure to ionizing irradiation. METHODS:Both repairable and irreparable radiation damage to DNA were assumed to occur by independent (Poisson) processes, at intensiti...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138600
更新日期：2000-04-01 00:00:00
abstract：PURPOSE:Caveolin-1 is a membrane protein highly expressed in many tumors and plays an important role in tumor progression and metastasis. This review describes the structure of the Caveolin-1 protein and its pre-clinical and clinical significance, demonstrating that Caveolin-1 is a novel biomarker for radioresistance w...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2016.1222096
更新日期：2016-12-01 00:00:00
abstract：:Radiation-resistant L5178Y-R (LY-R) and radiation-sensitive L5178Y-S (LY-S) murine lymphoma cells were X-irradiated and their free Ca2+ concentration was examined with Fura-2 in Ca2(+)-free salt solution. The release of free Ca2+ from intracellular stores was linear between 10 and 60 min after irradiation (1-5 Gy X-ra...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014551481
更新日期：1990-07-01 00:00:00
abstract：:To characterize further the radiosensitivity of severe combined immunodeficiency (scid) mice, the induction of micronuclei (MN) in polychromatic erythrocytes as well as cell killing and translocation induction in stem cell spermatogonia was studied. Scid mice turned out to be clearly hypersensitive for X-ray-induced c...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514550661
更新日期：1995-05-01 00:00:00
