Abstract:
PURPOSE:It has been proposed previously that the ratio of complete to incomplete translocations as seen by fluorescence in situ hybridization (FISH), the S value, can be a cytogenetic fingerprint of exposure to radiation of different qualities. Results from a previous study suggested that the S value is approximately 10 for sparsely ionizing radiations such as X- and gamma-rays, and 2 for densely ionizing radiations. Based on FISH data of atomic-bomb (A-bomb) survivors, which showed an S value of 3.25, a significant neutron component to A-bomb radiation was suggested. To examine the possibility, the present in vitro study was conducted using X-rays. MATERIALS AND METHODS:Human blood lymphocytes were exposed to X-rays and first metaphases were examined with FISH using DNA probes for chromosomes 1, 2 and 4. RESULTS:The S value was 3.16 for X-rays, which differs from approximately 10 as reported previously, and not larger than the 3.25 obtained from the blood lymphocytes of A-bomb survivors. CONCLUSIONS:S values seem to vary among laboratories even after exposure of cells to sparsely ionizing radiations. Data from this study show that S values are not a signature for a significant contribution of neutrons to the radiation dose received by A-bomb survivors in Hiroshima.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Nakano M,Kodama Y,Ohtaki K,Itoh M,Nakamura Ndoi
10.1080/095530099140799subject
Has Abstractpub_date
1999-01-01 00:00:00pages
47-9issue
1eissn
0955-3002issn
1362-3095journal_volume
75pub_type
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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