Protein gamma-radiolysis in frozen solutions is a macromolecular surface phenomenon: fragmentation of lysozyme, citrate synthase and alpha-lactalbumin in native or denatured states.

Abstract:

PURPOSE:To test whether radiolysis-induced fragmentation in frozen aqueous protein solution is dependent on solvent access to the surface of the protein or to the molecular mass of the polypeptide chain. MATERIALS AND METHODS:60Co gamma-irradiation of three proteins at -78 degrees C: lysozyme, citrate synthase and alpha-lactalbumin in their native state, with or without bound substrate, or denatured (random coil in urea/acid-denatured state). RESULTS:By SDS-polyacrylamide gel electrophoresis/analysis of the protein-fragmentation process, it was found that for a given protein D37 values (dose to decrease the measured amount of protein, with an unaltered polypeptidic chain, to 37% of the initial amount) varied according to the state of the protein. D37 for denatured proteins was always much smaller than for native states, indicating a greater susceptibility to fragmentation. In urea, contrary to the native state, no well-defined fragments were observed. Radiolysis decay constants (K= 1/D37) increased with solvent-accessible surface area of these proteins estimated from their radii of gyration in the various states. This is shown also in previous data on native or SDS-denatured proteins. Denatured proteins which have a large surface area exposed to the solvent compared with native ones are more fragmented at equal doses. CONCLUSIONS:It is concluded that D37 is directly related to the exposed surface area and not to the molecular mass of the polypeptide chain.

journal_name

Int J Radiat Biol

authors

Audette M,Chen X,Houée-Levin C,Potier M,Le Maire M

doi

10.1080/095530000138349

subject

Has Abstract

pub_date

2000-05-01 00:00:00

pages

673-81

issue

5

eissn

0955-3002

issn

1362-3095

journal_volume

76

pub_type

杂志文章
  • Correlation between cell reproductive death and chromosome aberrations assessed by FISH for low and high doses of radiation and sensitization by iodo-deoxyuridine in human SW-1573 cells.

    abstract:PURPOSE:To study the relationship between cell reproductive death and exchange frequency in SW-1573 human lung tumour cells with and without incorporated iodo-deoxyuridine (IdUrd) following irradiation of plateau-phase cultures with y-rays. METHOD:Linear-quadratic (LQ) analysis was performed for the data on clonogenic...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/095530099140474

    authors: Franken NA,Ruurs P,Ludwików G,van Bree C,Kipp JB,Darroudi F,Barendsen GW

    更新日期:1999-03-01 00:00:00

  • Role of p53 mutations, protein function and DNA damage for the radiosensitivity of human tumour cells.

    abstract:PURPOSE:The tumour suppressor protein p53 is considered to have an impact on the radiosensitivity of tumour cells. However, this concept does not easily translate to the tumour sensitivity in the clinics. The aim of the present study was to determine whether a functional or dysfunctional p53 is associated with a sensit...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/09553000310001642902

    authors: Böhnke A,Westphal F,Schmidt A,El-Awady RA,Dahm-Daphi J

    更新日期:2004-01-01 00:00:00

  • Fullerenes as a new class of radioprotectors.

    abstract:PURPOSE:To evaluate the radioprotective activity of C3, a regioisomer of water-soluble carboxyfullerene and a potent free radical scavenger, on both normal and tumour cells. MATERIALS AND METHODS:The murine committed bone-marrow stem cells for both granulocytes and monocytes (GM-CFC) were used to represent normal cell...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/095530001750068966

    authors: Lin HS,Lin TS,Lai RS,D'Rosario T,Luh TY

    更新日期:2001-02-01 00:00:00

  • The formation of strand breaks in DNA after high-LET irradiation: a comparison of data from in vitro and cellular systems.

    abstract::This paper presents a summary of our understanding to date of the formation of DNA strand breaks induced by highly energetic particle beams (high-LET radiation). We have compared our own recent data on the formation of strand breaks induced in DNA in an aqueous solution with our previous data and those of others avail...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审

    doi:10.1080/09553009014551431

    authors: Roots R,Holley W,Chatterjee A,Irizarry M,Kraft G

    更新日期:1990-07-01 00:00:00

  • Responses of the bacterial community to chronic gamma radiation in a rice paddy ecosystem.

    abstract:PURPOSE:Public concern about radiation protection of the environment and non-human species is increasing. The potential impact of chronic gamma radiation on the bacterial community in a rice paddy ecosystem was investigated. MATERIALS AND METHODS:Paddy field microcosm samples were continuously exposed at a dose rate o...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.3109/09553002.2010.549534

    authors: Ishii N,Fuma S,Tagami K,Honma-Takeda S,Shikano S

    更新日期:2011-07-01 00:00:00

  • Genetic changes in progeny of bystander human fibroblasts after microbeam irradiation with X-rays, protons or carbon ions: the relevance to cancer risk.

    abstract:PURPOSE:Radiation-induced bystander effects have important implications in radiotherapy. Their persistence in normal cells may contribute to risk of health hazards, including cancer. This study investigates the role of radiation quality and gap junction intercellular communication (GJIC) in the propagation of harmful e...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.3109/09553002.2014.950715

    authors: Autsavapromporn N,Plante I,Liu C,Konishi T,Usami N,Funayama T,Azzam EI,Murakami T,Suzuki M

    更新日期:2015-01-01 00:00:00

  • Marrow stromal cell recovery after radiation-induced aplasia in mice.

    abstract:PURPOSE:The identification of fibroblast-like cells of the marrow stroma by means of alkaline phosphatase (ALP) cytochemistry reveals delicate ALP-positive structures interspersed among haematopoietic cells and arranged in a loosely meshed network. These cells are often referred to as 'reticular' cells and the network ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/0955300031000085740

    authors: Almohamad K,Thiry A,Hubin F,Belaid Z,Humblet C,Boniver J,Defresne MP

    更新日期:2003-04-01 00:00:00

  • Localization of chromosome breakpoints induced by AluI and BamHI in Chinese hamster ovary cells treated in the G1 phase of the cell cycle.

    abstract::Intact Chinese hamster ovary cells were exposed to the restriction endonucleases (REs) AluI or BamHI. In metaphase spreads from these cells, 300 breakpoints per RE were localized in G-banded chromosome type aberrations (dicentrics, translocations, rings, terminal and interstitial deletions). The majority of breakpoint...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/09553009514551401

    authors: Folle GA,Obe G

    更新日期:1995-10-01 00:00:00

  • Influence of heat on Na(+)-dependent HCO3-/Cl- exchanger function in a thermoresistant cell line.

    abstract::During previous studies on the relationship between thermosensitivity and intracellular pH (pHi) regulation in mammalian cells, we observed that the thermoresistant TR-4 cells appeared to be resistant to manipulations of the Na+/H+ antiport, one of the two primary membrane regulators of pHi. We hypothesized that this ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/095530096144969

    authors: Liu FF,Lee C,Li JH

    更新日期:1996-10-01 00:00:00

  • International coordination of research: EPRI International Dose Effect Alliance.

    abstract::Purpose: The key to increasing our understanding of low dose effects of ionizing radiation is cooperation and communication between research organizations around the world. The purpose of this article is to describe the Electric Power Research Institute (EPRI) program for such cooperation. Materials and methods: EPRI ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/09553002.2019.1569770

    authors: Cool DA

    更新日期:2019-10-01 00:00:00

  • The New Zealand white rabbit animal model of acute radiation syndrome: hematopoietic and coagulation-based parameters by radiation dose following supportive care.

    abstract:PURPOSE:Animal models that accurately reflect human responses to radiation injury are needed for advanced mechanistic investigation and development of effective therapeutics. The rabbit is an established animal model accepted by the FDA for studies of cardiovascular disease, lipid metabolism, the development of anticoa...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/09553002.2020.1820606

    authors: Paredes A,Lindeblad M,Patil R,Neal MD,Hong Y,Smith B,Nanda JP,Mousafeiris V,Moulder J,Bosland MC,Lyubimov A,Bartholomew A

    更新日期:2020-10-26 00:00:00

  • Characterization of the specific response to serotonin of mouse tumour-feeding arterioles.

    abstract:PURPOSE:To investigate the role of tumour versus non-tumour factors in the specific response to serotonin (5-HT) of tumour-feeding arterioles (TFA). MATERIALS AND METHODS:Using mouse models of intra-vital microscopy, the response to topical administration of 5-HT was studied in arterioles feeding tumours: fibrosarcoma...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/095530098141519

    authors: Laemmel E,Stücker O,Darmon PL,Vicaut E

    更新日期:1998-09-01 00:00:00

  • Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells: a boron neutron capture therapy study.

    abstract:PURPOSE:The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. MATERIALS AND METHODS:Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometr...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.3109/09553002.2014.901579

    authors: Gifford I,Vreeland W,Grdanovska S,Burgett E,Kalinich J,Vergara V,Wang CK,Maimon E,Poster D,Al-Sheikhly M

    更新日期:2014-06-01 00:00:00

  • Radioprotective influence of Mentha piperita (Linn) against gamma irradiation in mice: Antioxidant and radical scavenging activity.

    abstract:PURPOSE:To evaluate the radiomodulatory influence of a leaf extract of Mentha piperita (Linn) on hepatic antioxidant status and lipid peroxidation in Swiss albino mice. MATERIALS AND METHODS:Animals were given either double distilled water or leaf extract of M. piperita orally (1 g/kg bwt/day) once a day for three con...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,收录出版

    doi:10.1080/09553000600771523

    authors: Samarth RM,Panwar M,Kumar M,Kumar A

    更新日期:2006-05-01 00:00:00

  • Cell survival characteristics of a human colon adenocarcinoma cell line after photodynamic treatment: a comparison of Photofrin II and TPPS.

    abstract::A comparison has been made of the in vitro light-activated drug cytotoxicities of two different porphyrin compounds, Photofrin II and TPPS4. An early passage human colon adenocarcinoma cell line, WiDr, has been exposed to either drug for 24 h, the excess drug washed from the cells and the cells irradiated with light u...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/09553008814552061

    authors: West CM,Moore JV

    更新日期:1988-10-01 00:00:00

  • Chironomus ramosus larvae exhibit DNA damage control in response to gamma radiation.

    abstract:PURPOSE:Chironomus ramosus is one of the recently reported radiotolerant insects. Salivary gland cells of fourth instar larvae respond to ionizing radiations with increases in the levels of antioxidant enzymes and chaperone proteins. Here we made an attempt to study the state of nuclear DNA after exposure of larvae to ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.3109/09553002.2015.1062572

    authors: Datkhile KD,Gaikwad PS,Ghaskadbi SS,Mukhopadhyaya R,Nath BB

    更新日期:2015-01-01 00:00:00

  • Stable chromosomal aberrations in haemopoietic stem cells in the blood of radiation accident victims.

    abstract:PURPOSE:The detection of long-term persistent chromosome aberrations in circulating haemopoietic stem cells after accidental radiation exposure. MATERIAL AND METHODS:Peripheral blood samples from highly exposed persons were collected 7-25 years after the radiation accidents in Moscow (1971), Kazan (1975) and Chernobyl...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/095530099139395

    authors: Kreja L,Greulich KM,Fliedner TM,Heinze B

    更新日期:1999-10-01 00:00:00

  • Gold nanoparticles as dose-enhancement agent for kilovoltage X-ray therapy of melanoma.

    abstract:PURPOSE:Melanoma is mainly treated by surgery and rarely with radiation because of the high radioresistance of this tumor. Nevertheless, radiotherapy is the preferred treatment modality for unresectable lesions and avoiding cosmetic disfigurement caused by surgical excision. This study investigated the therapeutic adva...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/09553002.2017.1276309

    authors: Kim SR,Kim EH

    更新日期:2017-05-01 00:00:00

  • Cancer stem cells and radiotherapy.

    abstract:PURPOSE:The present work summarises the history and current status of research into the importance of cancer stem cells for radiobiological research and for clinical radiation oncology. An effort is made to differentiate clonogenicity from stemness of cancer cells. CONCLUSION:In radiooncology, cancer stem cells have b...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审

    doi:10.1080/09553000902836404

    authors: Baumann M,Krause M,Thames H,Trott K,Zips D

    更新日期:2009-05-01 00:00:00

  • Radiation response of mouse lymphoid and myeloid cell lines. Part III. Different signals can lead to apoptosis and may influence sensitivity to killing by DNA double-strand breakage.

    abstract::We have examined the timing of gamma-irradiation-induced death in relation to cell cycle progression using a panel of mouse lymphoid or myeloid cell lines. Death was found to occur immediately after irradiation ('rapid interphase' death), or after arrest in G2 phase ('delayed interphase' death). In part II of this ser...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/09553009414550261

    authors: Radford IR,Murphy TK

    更新日期:1994-02-01 00:00:00

  • Putative determinants of the cellular response to hyperthermia.

    abstract::Recently, it has been demonstrated that two different thermal resistant states found in Chinese hamster cells, one transient, associated with thermotolerance, and the other permanent, associated with the increased expression of the cognate member of the hsp 70 family, are characterized by faster recovery from heat-ind...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/09553009314450751

    authors: Laszlo A,Davidson T,Hu A,Landry J,Bedford J

    更新日期:1993-05-01 00:00:00

  • Critical role of RecA and RecF proteins in strand break rejoining and maintenance of fidelity of rejoining following gamma-radiation-induced damage to pMTa4 DNA in E. coli.

    abstract:PURPOSE:This study was undertaken to understand the roles of RecA and RecF proteins in strand break rejoining and maintenance of fidelity of the process following exposure of E. coli to gamma-radiation in vivo. MATERIALS AND METHODS:A plasmid DNA construct, pMTa4, was transformed into isogenic repair proficient (wild)...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/09553000601121140

    authors: Sharan RN,Ryo H,Nomura T

    更新日期:2007-02-01 00:00:00

  • Induction and persistence of chromosomal exchanges in mouse bone marrow cells following whole-body exposure to X-rays.

    abstract:PURPOSE:To investigate the induction and persistence of chromosome aberrations in mouse bone marrow cells after X-ray exposure and to detect differential involvement of individual chromosomes in translocations. MATERIALS AND METHODS:Male and female Swiss mice were exposed to 1 and 3 Gy of X-rays. Chromosome aberration...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/095530099139593

    authors: Xiao Y,Darroudi F,Grigorova M,Natarajan AT

    更新日期:1999-09-01 00:00:00

  • Comet Assay analysis of DNA strand breaks after exposure to the DNA-incorporated Auger Electron Emitter Iodine-125.

    abstract:PURPOSE:Ionizing radiation causes various types of DNA damage e.g. single strand breaks (SSB) and double strand breaks (DSB), whereby the SSB/DSB ratio is shifted toward the DSB with increasing LET. For the DNA-incorporated Auger electron emitter Iodine-125 a SSB/DSB ratio of 5.4:1 is calculated based on computer simul...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/09553002.2020.1851059

    authors: Unverricht-Yeboah M,Holtmann K,Kriehuber R

    更新日期:2020-12-03 00:00:00

  • Theoretical predictions on the equality of radiation-produced dicentrics and translocations detected by chromosome painting.

    abstract::Existing models of chromosome aberrations produced by ionizing-radiation predict equal numbers of dicentrics and translocations if the dose is so low that complex aberrations can be ignored. We show that, for a specific subset of aberrations detected by FISH, dicentric/translocation equality is predicted even at highe...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/095530096145977

    authors: Lucas JN,Chen AM,Sachs RK

    更新日期:1996-02-01 00:00:00

  • L5178Y sublines: a look back from 40 years. Part 2: response to ionizing radiation.

    abstract::The aim was to review and summarize the results of 40 years of study concerning the response to ionizing radiation of the pair of L5178Y (LY) sublines, LY-R and LY-S, that differ in sensitivity to various DNA-damaging agents, among them X- and gamma-rays. The reviewed data indicate the key importance of DNA damage rep...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审

    doi:10.1080/09553000500143534

    authors: Szumiel I

    更新日期:2005-05-01 00:00:00

  • The analysis of the densely populated patterns of radiation-induced foci by a stochastic, Monte Carlo model of DNA double-strand breaks induction by heavy ions.

    abstract:PURPOSE:To resolve the difficulty in counting merged DNA damage foci in high-LET (linear energy transfer) ion-induced patterns. MATERIALS AND METHODS:The analysis of patterns of RIF (radiation-induced foci) produced by high-LET Fe and Ti ions were conducted by using a Monte Carlo model that combines the heavy ion trac...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.3109/09553001003717175

    authors: Ponomarev AL,Huff J,Cucinotta FA

    更新日期:2010-06-01 00:00:00

  • Delayed effects of prenatal low-dose irradiation in the white matter of the rat brain.

    abstract:PURPOSE:This study is part of a general search for the anatomical bases of the severe mental retardation syndrome caused by prenatal irradiation. More specifically, it seeks reasons for the high radiosensitivity of a white matter area, the cingulum of the corpus callosum. MATERIALS AND METHODS:Pregnant primiparous Wis...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/095530099139494

    authors: Reyners H,Gianfelici de Reyners E,Yan J,De Saint-Georges L,Desaintes C

    更新日期:1999-10-01 00:00:00

  • Time course for the hazard of radiation-induced pneumonitis death in mice.

    abstract::The form of the hazard function for radiation-induced pneumonitis death in mice was investigated. 'Hazard' refers to the instantaneous failure rate at a specified time, conditional upon non-failure to that time. Thus, the hazard function describes the time profile for the risk of pneumonitis death among still-survivin...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/09553009214552551

    authors: Tucker SL,Travis EL

    更新日期:1992-11-01 00:00:00

  • Intrinsic radiosensitivity and PLD repair in osteosarcoma cell lines.

    abstract::The response to radiation of seven osteosarcoma cell lines was analysed by in vitro colony-forming assay and compared with that of eight human fibroblast strains. The values of D0, the surviving fraction after 2 Gy (S2Gy), and the mean inactivation dose (D) of osteosarcoma cells in log-phase culture were significantly...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章

    doi:10.1080/09553009214552351

    authors: Sugimoto M,Toguchida J,Kotoura Y,Yamamuro T,Utsumi H

    更新日期:1992-10-01 00:00:00