Abstract:
PURPOSE:Following ionizing radiation exposure, intestinal crypt regeneration is possible but it is still not known if regenerating crypts give rise to differentiated functional epithelial cells on villi. The aim of this study was to demonstrate that irradiated progeny of enterocytic precursor cells are capable of proliferation and subsequent differentiation using the HT-29?cell line. MATERIALS AND METHODS:Cells were cultured, irradiated (5 Gy or 10 Gy) and incubated in the presence or absence of butyrate (5 mM). Cell numbers, cell cycle parameters, alkaline phosphatase (ALP) activity, occludin labelling and gene expression were determined at different times post-exposure. RESULTS:Butyrate-induced inhibition of cell growth and arrest in G0 phase was comparable in both sham and irradiated cells in addition to similar development of ALP activity and expression. Cells also formed a monolayer with tight junctions post-irradiation. Butyrate-stimulated modulation of integrin expression during differentiation was unchanged after radiation exposure. Genes known to be implicated in differentiation mechanisms, i.e., growth and transcription factors (vascular Epidermal Growth Factor, v-EGF ; Activating Transcription Factor 4, ATF4), cell cycle genes (Cyclin-Dependent Kinase Inhibitor 1A, CDKN1A/p21(Cip1/waf1)), were studied. Most responded similarly to the differentiation stimulus whether irradiated or not. CONCLUSION:These results demonstrate that irradiated HT-29 cells still respond to butyrate to form a differentiated, functional epithelium.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Haton C,Lebrun F,Benderitter M,Griffiths NMdoi
10.1080/09553000500091923subject
Has Abstractpub_date
2005-03-01 00:00:00pages
211-20issue
3eissn
0955-3002issn
1362-3095pii
T073GT80018P4M07journal_volume
81pub_type
杂志文章abstract::Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently dete...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214552621
更新日期:1992-12-01 00:00:00
abstract::Solid human tumours contain areas with low oxygen tension (pO2). For bioreductive drugs it is important to define the cytotoxic effect according to drug concentration and to clinically relevant pO2. In this study, the pO2 dependence of the survival of three human cell lines (HRT 18, Na11 +, and MEWO), exposed to tirap...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514550261
更新日期:1995-02-01 00:00:00
abstract:PURPOSE:The prolonged delivery times associated with intensity modulated radiation therapy (IMRT) may reduce treatment effectiveness of radiation therapy for cancers with short repair half-times. In this study, in vitro radiation experiments with DU-145 prostate cancer cells were designed to quantify the half-time of s...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802061285
更新日期:2008-06-01 00:00:00
abstract:PURPOSE:To determine the radiosensitivity difference of human Cluster of Differentiation (CD)4(+)CD25(+) regulatory T cells (Treg) and effector T cells to low dose gamma ray and elucidate the underlying mechanisms in vitro. MATERIALS AND METHODS:Blood samples were collected from five health subjects and five patients ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.518208
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:Caffeine (1,3,7-trimethyl xanthine), a dietary component, has been shown to have widely varying effects on DNA damage induced by UV and ionizing radiation, depending upon pre- or post-irradiation administration and its concentration. Caffeine administered post-UV irradiation is known to inhibit enzymatic repair...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110034649
更新日期:2001-05-01 00:00:00
abstract::Purpose: To study the response of neuronal-like cells to an applied static or low-frequency magnetic field. Materials and methods: Fourier Transform Infrared (FTIR) Spectroscopy was used to investigate the overall behavior of SH-SY5Y neuronal-like cells exposed to a static or 50 Hz magnetic fields at intensities up to...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1571256
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:Ionizing radiation causes various types of DNA damage e.g. single strand breaks (SSB) and double strand breaks (DSB), whereby the SSB/DSB ratio is shifted toward the DSB with increasing LET. For the DNA-incorporated Auger electron emitter Iodine-125 a SSB/DSB ratio of 5.4:1 is calculated based on computer simul...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1851059
更新日期:2020-12-03 00:00:00
abstract:PURPOSE:Biomarkers of ionising radiation exposure are useful in a variety of scenarios, such as medical diagnostic imaging, occupational exposures, and spaceflight. This study investigates to what extent microRNA (miRNA) expression signatures in mouse peripheral blood can be used as biomarkers for exposures to radiatio...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.549537
更新日期:2011-07-01 00:00:00
abstract:PURPOSE:The extreme radiotoxicity of Auger electrons and their exquisite capacity to irradiate specific molecular sites has prompted scientists to extensively investigate their radiobiological effects. Their efforts have been punctuated by quadrennial international symposia that have focused on biophysical aspects of A...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553000802395527
更新日期:2008-12-01 00:00:00
abstract::Purpose: Cancer is one of the most common causes of mortality and morbidity worldwide. Vaccines have been emerged as an attractive approach for their capacity of eliciting long-term immune response targeting cancer cells. Attenuated avirulent Toxoplasma gondii stimulate immunity and activate antitumor cells thereby el...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1739772
更新日期:2020-06-01 00:00:00
abstract:PURPOSE:To assess changes in mitochondrial morphology and mitophagy induced by simulated sunlight irradiation (SSI) and how these changes are modulated by mitochondrial activity and energy source. MATERIALS AND METHODS:Human malignant amelanotic melanoma A375 cells were pre-treated with either a mitochondrial activity...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.556175
更新日期:2011-05-01 00:00:00
abstract::The chemical instability of DNA under physiological conditions requires that cells have highly developed processes for repairing stochastic single-strand damage. It is proposed here that provided ionizing-radiation-induced single-strand damage does not occur at a rate sufficient to perturb the dynamic steady state bet...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114552421
更新日期:1991-10-01 00:00:00
abstract::Resolving the molecular mechanisms of radiation oncogenesis represents an important but daunting challenge in radiation research. This brief review outlines the principal oncogenic mechanisms that need to be considered in the context of radiation effects on the genome, how these might relate to specific gene and chrom...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009414550081
更新日期:1994-01-01 00:00:00
abstract::Radioiodine ((131)I) induced a dose- and time-dependent increase in DNA single-strand breaks (DNA-ssb) in human (G0) mononuclear blood cells (MNC) in vitro. Incubation of MNC with 22MBq (131)I/ml at 4 degrees C caused a linear, time-dependent induction of DNA-ssb (increase in elution rate: 24.7 x 10(-3) h(-1) per 100 ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143103
更新日期:1997-11-01 00:00:00
abstract:PURPOSE:To investigate physical and biophysical properties of proton tracks 1 keV-300 MeV using Monte Carlo track structure methods. MATERIALS AND METHODS:We present model calculations for cross sections and methods for simulations of full-slowing-down proton tracks. Protons and electrons were followed interaction-by-...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.518204
更新日期:2011-02-01 00:00:00
abstract::High doses of rHIL-1 protected C3H/HeN mice against lethal radiation damage. The optimum time of radioprotection was found when this cytokine was administered at 4 h and at 20 h before irradiation. The dose reduction factors when the IL-1 was administered at those times were about 1.30. The radioprotection at the opti...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914551621
更新日期:1989-10-01 00:00:00
abstract:PURPOSE:The need to determine a reliable relative biological effectiveness (RBE) value for alpha exposures has become important as reports remain controversial. Although a radiation-weighting factor of 20 has been designated for alpha particles, uncertainty exists on realistic value of the RBE of alpha radiation. The a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2015.1047986
更新日期:2015-08-01 00:00:00
abstract::Single crystals of cytosine hydrochloride with thiocytosine as an impurity were found suitable for the study of a possible new mechanism of long-range migration of energy deposited by ionizing radiation. In a crystal containing thiocytosine, two kinds of chlorine-containing paramagnetic centres are present that are co...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414550891
更新日期:1994-07-01 00:00:00
abstract::Cells use substrates such as glucose and glutamine to provide energy for repair of radiation damage. Glutaminolysis and glycolysis were inhibited by aminooxyacetic acid (AOAA) and 2-deoxyglucose (2DG), respectively, to inhibit metabolism of these substrates in order to determine the effect on radiation response of CHO...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098142419
更新日期:1998-03-01 00:00:00
abstract::Four of the most radiosensitive xrs variants of CHO-K1 cells, obtained after mutagenizing treatment with EMS, have been studied in detail over three to five decades of cell survival. Although these lines were initially reported to have very steep exponential survival curves, and to vary in sensitivity between themselv...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914550651
更新日期:1989-04-01 00:00:00
abstract::Chromosomes are located within the interphase nucleus in regions called domains. Using fluorescence in situ hybridization with whole chromosome paints, a pain of homologous chromosomes can be visualized as two discrete domains and their relative spatial location determined. This study examines the effects of an ionizi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143293
更新日期:1997-09-01 00:00:00
abstract:PURPOSE:To investigate the possible role of vitamin E, coenzyme Q10 and rutin in ameliorating the biochemical changes in brain and serum induced by cerebral ischemia/reperfusion (I/R) in whole body γ-irradiated rats. MATERIALS AND METHODS:Cerebral ischemia was induced in male Wistar rats (either irradiated or non-irra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.501844
更新日期:2010-12-01 00:00:00
abstract:PURPOSE:The behavioral responses of planaria to the exposures of a range of concentrations of morphine (μM to attoM) or the μ-opiate antagonist naloxone or to either of these compounds and a burst-firing magnetic field (5 μT) were studied. MATERIAL AND METHODS:The locomotor velocity (LMV) of planaria was measured afte...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.911421
更新日期:2014-10-01 00:00:00
abstract::The effects of a low dose (0.1-20 mGy) preirradiation with X-rays followed by a higher dose (2 Gy) of the same radiation on the recovery of the genetic damage induced as dominant lethals in mature oocytes (stage 14) of different strains of Drosophila melanogaster were investigated. The response was shown to be depende...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414550501
更新日期:1994-04-01 00:00:00
abstract:PURPOSE:Theoretical modelling techniques are often used to simulate the action of ionizing radiations on cells at the nanometre level. Using monoenergetic vacuum-UV (VUV) radiation to irradiate DNA either dry or humidified, the action spectra for the induction of DNA damage by low energy photons and the role of water a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050028913
更新日期:2000-06-01 00:00:00
abstract:PURPOSE:The molecular basis of gene regulation in cells exposed to ionising radiation is not fully understood. Gene regulation occurs at the transcriptional and post-transcriptional levels. Recent studies have suggested that micro-RNA (miRNA) plays a significant role at the post-transcriptional gene regulation. miRNA a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553001003734568
更新日期:2010-07-01 00:00:00
abstract::The recently released data on cancer incidence in the Japanese atomic bomb survivors are analysed using a variety of relative risk models which take account of errors in estimates of dose to assess the dose-response at low doses. For all solid cancers analysed together there is a significant positive dose-response (at...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145364
更新日期:1996-07-01 00:00:00
abstract:PURPOSE:Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments describ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138844
更新日期:2000-02-01 00:00:00
abstract:PURPOSE:To study the relationship between cell reproductive death and exchange frequency in SW-1573 human lung tumour cells with and without incorporated iodo-deoxyuridine (IdUrd) following irradiation of plateau-phase cultures with y-rays. METHOD:Linear-quadratic (LQ) analysis was performed for the data on clonogenic...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099140474
更新日期:1999-03-01 00:00:00
abstract:PURPOSE:Ionizing radiation induces DNA damage, some of which are present in clusters, defined as two or more lesions within one to two helical turns of DNA by passage of a single radiation track. These clusters are thought to contribute to the detrimental effects of radiation, in part due to the compromised repair of c...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.899449
更新日期:2014-06-01 00:00:00