Abstract:
:A one-carbon degradation of long-chain fatty acids, which was found to occur in the brains of rats in vivo, has been investigated in a brain microsomal fraction in vitro. Decarboxylation of the alpha-hydroxy acid, a possible intermediate product between the substrate and the next shorter acid, in the presence of brain microsomal fraction was enhanced by ATP, NAD, and a dialyzable fraction from the supernatant fraction. The cofactor requirement for the decarboxylation of the alpha-hydroxy acids provided by the dialyzable fraction can be met by several reducing agents or ferrous ion. The effectiveness of several possible cofactors for the decarboxylation of alpha-hydroxy acids has been evaluated.It is concluded that the decarboxylation of the alpha-hydroxystearic acid may be a reaction with molecular oxygen catalyzed by an oxidase or oxygenase that requires iron in the reduced state for activity. The possibility that the reaction proceeds through an alpha-keto acid intermediate has been examined in the light of new knowledge of the conditions for decarboxylation. It is concluded that a short-lived keto acid is a possible intermediate. Definitive proof however is lacking because the characteristics of the reaction require that such an intermediate decarboxylate without dissociating from the enzyme.
journal_name
Lipidsjournal_title
Lipidsauthors
Macdonald RC,Mead JFdoi
10.1007/BF02531201subject
Has Abstractpub_date
1968-05-01 00:00:00pages
275-83issue
3eissn
0024-4201issn
1558-9307journal_volume
3pub_type
杂志文章相关文献
LIPIDS文献大全abstract::In the present study, changes in phospholipid compositions of liver microsomes, erythrocyte membranes, platelets, aorta, cardiac muscle and brain of rats fed olive oil were compared with those of rats fed sunflower oil. Four groups of rats starting at weaning were fed for four weeks a basal diet containing 5 or 25% ol...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536252
更新日期:1994-12-01 00:00:00
abstract::Conjugated linoleic acid (CLA) is a collective term that describes different isomers of linoleic acid with conjugated double bonds. Although the main dietary isomer is 9cis,11trans-18:2, which is present in dairy products and ruminant fat, the biological effects of CLA generally have been studied using mixtures in whi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0759-8
更新日期:2001-06-01 00:00:00
abstract::Lipid-enriched diets have been related to a high cancer incidence in experimental animals for many years, and more recently, to assorted defects on the immune response. We investigated the effect of incubating human or murine (C3H/HEJ) lymphocytes with saturated (16:0) and unsaturated (18:1, 18:2, 18:3, 20:4) fatty ac...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534659
更新日期:1982-10-01 00:00:00
abstract::Experimental studies have suggested an important role of the spleen in lipid metabolism, although with controversial results. Our purpose was to analyze the effect of a nutritionally balanced (NB) diet and a diet containing pork fat (PF) as source of lipids on the lipid profile of rats submitted to splenic surgery. Si...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-009-3302-x
更新日期:2009-06-01 00:00:00
abstract::The aim of this study was to develop a simple method for simultaneous determination of selected cis/cis PUFA-LNA (18:2), ALA (18:3), GLA (18:3), EPA (20:5), and DHA (22:6) by silver ion high-performance liquid chromatography coupled to a diode array detector (Ag-HPLC-DAD). The separation was performed on three Luna SC...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-016-4133-1
更新日期:2016-04-01 00:00:00
abstract::With the passage of the U.S. Dietary Supplement Health and Education Act of 1994, dehydroepiandrosterone (DHEA, 5-androsten-3beta-ol-17-one) has become widely available, and a large and growing market has developed for this "fountain of youth." DHEA has been shown to have significant beneficial effects in animals, whi...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-000-0529-7
更新日期:2000-03-01 00:00:00
abstract::We evaluated the effects of dietary lipid class (phospholipid vs. neutral lipid) and level of n-3 long-chain PUFA (LC-PUFA) on the growth, digestive enzymatic activity, and histological organization of the intestine and liver in European sea bass larvae. Fish were fed from the onset of exogenous feeding at 7 to 37 d p...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-005-1422-0
更新日期:2005-06-01 00:00:00
abstract::This study was undertaken to investigate the total plasma fatty acid composition and the relationship between plasma triacylglycerol (TG) levels and liver delta 9 desaturase activity in mice fed n-3 and/or n-6 fatty acid or hydrogenated coconut oil (HCO) (maximum 25 mg/g) supplemented diets. Generally, plasma TG level...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536312
更新日期:1993-04-01 00:00:00
abstract::An improved headspace capillary gas chromatographic (GC) method was developed to measure the oxidative susceptibility of human red blood cell (RBC) membranes. This method analyzed volatile peroxidation products of both n-6 (hexanal and pentane) and n-3 (propanal) polyunsaturated fatty acids. Oxidative susceptibility t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536076
更新日期:1991-06-01 00:00:00
abstract::Ceramide 2-aminoethylphosphonate (CAEP), a sphingophosphonolipid containing a carbon-phosphorus bond, is frequently found in marine organisms and has a unique triene type of sphingoid base in its structure. CAEP has not been evaluated as a food ingredient, although it is generally contained in Mollusca organisms such ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-017-4239-0
更新日期:2017-04-01 00:00:00
abstract::Adult male marmoset monkeys were fed eicosapentaenoic acid (20:5n-3) as the ethyl ester in diets containing either 32% (reference diet, no added cholesterol) or 7% (atherogenic diet with 0.2% added cholesterol) linoleic acid (18:2n-6) for 30 wk. No changes were seen in the level of phosphatidylcholine (PC) or phosphat...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536173
更新日期:1992-03-01 00:00:00
abstract::A protocol using a dry column method was modified for the extraction of total lipids and the simultaneous separation and quantitation of neutral and polar lipids in human milk. The triacylglycerol, cholesterol, phospholipid and vitamin E contents of the lipid extracts were determined and compared with lipids extracted...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535216
更新日期:1989-08-01 00:00:00
abstract::There is convincing evidence that normal cultured human keratinocytes possess a 15-lipoxygenase activity which, however, does not appear to manifest itself without cell membrane damage. When "activated", this enzyme transforms arachidonic acid into 15-hydroxyeicosatetraenoic acid (15-HETE), and linoleic acid into 13-h...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536012
更新日期:1990-10-01 00:00:00
abstract::The impact of starch sources differing in their velocities of ruminal degradation on the milk fat of dairy cows was studied. The animals received diets containing a slowly degradable (potatoes) or rapidly degradable (wheat) starch concentrate (40% of the dry matter) in a total mixed diet. Milk fat was the only animal ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-004-1196-4
更新日期:2004-01-01 00:00:00
abstract::The purpose of this study was to investigate in healthy humans the effect of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) intake, alone or in combination with dL-alpha-tocopherol acetate (vitamin E) supplements on lipid peroxidation. Eighty men were randomly assigned in a double-blind fashion to take dai...
journal_title:Lipids
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s11745-997-0068-2
更新日期:1997-05-01 00:00:00
abstract::The effect of lecithin on jejunal absorption of fatty acids and octadecenoylglycerol was studied in healthy volunteers with a jejunal perfusion system which excluded pancreatic and biliary secretions from the test segment. Lecithin significantly reduced the absorption of oleic acid (P less than 0.05) and octadecenoylg...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533424
更新日期:1979-04-01 00:00:00
abstract::Fecal bile acid and neutral sterol patterns were studied in eight healthy adult volunteers who were challenged with Vibrio cholerae classical Ogawa 395 strain in the course of vaccine development studies. Bacterial 7 alpha-dehydroxylation of cholic and chenodeoxycholic acids was not altered during experimentally induc...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535367
更新日期:1982-09-01 00:00:00
abstract::We describe a method for the quantitative analysis of molecular species of diacylglycerol and alkylacylglycerol as their diradylglycerobenzoate derivatives. Synthetic internal standards were used to provide quantitative determinations of the low levels of diacylglycerol and alkylacylglycerol and their individual molec...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544045
更新日期:1990-11-01 00:00:00
abstract::The oils from sclerotia or from suitable mycelial cultures ofClaviceps purpurea (ergot) contain up to 44% of ricinoleic acid but no free hydroxyl groups. This is due to the presence of, besides normal triglycerides, tetra-acid, penta-acid and hexa-acid triglycerides. These contain respectively one, two and three ricin...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02531871
更新日期:1966-05-01 00:00:00
abstract::Accurate quantification of the major classes and subfractions of human serum lipoproteins is an important analytical need in the characterization and evaluation of therapy of lipid and lipoprotein abnormalities. For calibrating the analytic ultracentrifuge (AnUC), we routinely use a Beckman calibration wedge cell with...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534491
更新日期:1984-07-01 00:00:00
abstract::Because of the known advantages of coupling high performance liquid chromatography with mass spectrometry (HPLC-MS) in biological fluids, studies on the reversed-phase HPLC-MS system for direct analysis of conjugated bile acids in human bile samples are described. Ten samples of gallbladder bile of apparently healthy ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534697
更新日期:1983-01-01 00:00:00
abstract::We have investigated the regulation of cholesterol synthesis in isolated human small intestine epithelial cells (enterocytes). It was established that the amount of cholesterol synthesized increased linearly with the incubation time and the number of cells in the incubation mixture; the synthesis was suppressed by 7-k...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535408
更新日期:1986-12-01 00:00:00
abstract::The purpose of this study was to compare fish intake and plasma phospholipid concentrations of n-3 fatty acids, in particular of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), among representative population samples of Québecers, James Bay Cree, and Inuit of Nunavik (Canada). The relationships between the...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-003-1070-4
更新日期:2003-04-01 00:00:00
abstract::CLA mixtures are now commercially available. They differ from each other with respect to their content of CLA isomers and their degree of purification. As a group of natural FA, CLA have been widely assumed to be safe. However, the suspected presence of both impurities and particular isomers might induce undesirable s...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-002-0995-y
更新日期:2002-11-01 00:00:00
abstract::The hypothesis that the essential oil from Tagetes minuta L. can interact with biological membranes was investigated by assessing its ability of perturbing the binding of a benzodiazepine [flunitrazepam (FNTZ)] to crude members from chick brains. The essential oil from T. minuta L. inhibited [3H]FNTZ specific binding ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536610
更新日期:1995-12-01 00:00:00
abstract::The de novo synthesis of choline and ethanolamine phosphoglycerides in brain microsomes from 18 month-old male rats was investigated in vitro by using labeled cytidine-5'-diphosphate choline and cytidine-5'-diphosphate ethanolamine as lipid precursors. The rate of synthesis of the two phospholipid classes was found to...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533507
更新日期:1979-11-01 00:00:00
abstract::The aim of the present work was to study the effect of season on phospholipids and triacylglycerols (TAG) of mantle and tentacles of female and male wild Sepia officinalis. The identified phospholipids were phosphatidylethanolamine (PtdEtn), phosphatidylcholine (PtdCho), phosphatidylserine (PtdSer), and phosphatidylin...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12137
更新日期:2019-02-01 00:00:00
abstract::An experiment examined delta9 desaturase activity and FA composition in subcutaneous adipose tissue in two differing breeds of cattle. Jersey-sired cattle had significantly higher rates of desaturase activity than Limousin-sired cattle (1.55 vs. 0.75 nmol/mg protein/min). This difference was also demonstrated by a low...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-003-1339-7
更新日期:2003-05-01 00:00:00
abstract::The fatty acid (FA) profile of oysters generally reflects the dietary FA composition. Moreover, incorporation of FA into tissues is modulated by various metabolic factors, and final composition will depend upon the dietary sources, cumulative intake, and oysters' development stage. Thus, the aim of this study was to a...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12177
更新日期:2019-09-01 00:00:00
abstract::Lecithin-cholesterol acyltransferase (LCAT, EC 2.3.1.43) was assayed in vitro in plasma from normal man, rat, rabbit and dog by following the formation of 14C-cholesteryl esters subsequent to labeling the plasma with 14C-cholesterol in vitro. In all species examined, various local anesthetics were found to inhibit LCA...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534370
更新日期:1980-10-01 00:00:00