A method for the quantitative analysis of molecular species of alkylacylglycerol and diacylglycerol.

Abstract:

:We describe a method for the quantitative analysis of molecular species of diacylglycerol and alkylacylglycerol as their diradylglycerobenzoate derivatives. Synthetic internal standards were used to provide quantitative determinations of the low levels of diacylglycerol and alkylacylglycerol and their individual molecular species in cultured cells. Diradylglycerols were isolated by thin-layer chromatography (TLC), converted to their benzoate derivatives and separated into subclasses by TLC. The molecular species of each subclass were analyzed by reversed-phase high performance liquid chromatography. Thirty-six species of diglyceride-type molecules were identified in Madin-Darby canine kidney cells. These cells were shown to contain 7.88 nmoles of diacylglycerol and 3.97 nmoles of alkylacylglycerol per mumole of phospholipid. Both subclasses contain predominantly monoenoic and saturated species. This technique should be valuable for studies examining the origin and metabolism of these important intracellular mediators.

journal_name

Lipids

journal_title

Lipids

authors

Warne TR,Robinson M

doi

10.1007/BF02544045

subject

Has Abstract

pub_date

1990-11-01 00:00:00

pages

748-52

issue

11

eissn

0024-4201

issn

1558-9307

journal_volume

25

pub_type

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