Simultaneous quantification of plant glyceroglycolipids including sulfoquinovosyldiacylglycerol by HPLC-ELSD with binary gradient elution.

Abstract:

:Membrane lipids of photosynthetic organisms consist of glycerophospholipids and glyceroglycolipids. We investigated a method for the simultaneous quantitative analysis of neutral and acidic lipids using HPLC-ELSD, and quantified monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and sulfoquinovosyldiacylglycerol (SQDG). Ten complex lipid classes were separated with a binary gradient system consisting of chloroform and methanol-acetone-water-acetic acid (30:60:9:1, v/v/v/v) with 0.3% triethylamine (pH 4), and were eluted within 16 min. The contents of SQDG in ten edible plants ranged from 3 to 101 mg/100 g, and were positively correlated to the neutral glyceroglycolipids contents.

journal_name

Lipids

journal_title

Lipids

authors

Yunoki K,Sato M,Seki K,Ohkubo T,Tanaka Y,Ohnishi M

doi

10.1007/s11745-008-3248-4

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

77-83

issue

1

eissn

0024-4201

issn

1558-9307

journal_volume

44

pub_type

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