Abstract:
:A mutant of Mortierella alpina, JT-180, which is defective in its delta12 desaturase activity but exhibits enhanced activities of delta5 and delta6 desaturases, produced a high proportion (up to 80%) of odd-chain FA when grown on 3% n-heptadecane. Following growth of the mutant on n-heptadecane, three unusual odd-chain fatty acyl residues were identified as 6,9-heptadecadienoic acid (17:2), 8,11 -nonadecadienoic acid (19:2), and 5,8,11-nonadecatrienoic acid (19:3) by means of GC-MS, MS-MS, and NMR analyses. The mycelial contents of these FA reached 20.3, 3.6, and 5.8 mg/g dry mycelia, respectively, when it was cultivated in medium comprising 4% (vol/vol) n-heptadecane and 1% (wt/vol) yeast extract, pH 6.0, at 28 degrees C for 7 d. The biosynthetic route (n-8 route) to 19:3 was presumed to mimic the n-9 route to Mead acid (20:3n-9) in mammals: 17:0 --> 9-17:1 --> 6,9-17:2 --> 8,11-19:2 --> 5,8,11-19:3.
journal_name
Lipidsjournal_title
Lipidsauthors
Zhang S,Sakuradani E,Shimizu Sdoi
10.1007/s11745-006-5011-zsubject
Has Abstractpub_date
2006-06-01 00:00:00pages
623-6issue
6eissn
0024-4201issn
1558-9307journal_volume
41pub_type
杂志文章相关文献
LIPIDS文献大全abstract::To see how the metabolism of specific phosphatidyl choline fractions might be affected when only a limited source of methyl groups was available, rats were fed for 7 days a low methionine, choline-deficient diet or one supplemented with either choline or methionine. Prior to killing, they were injected with -14C-methy...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534154
更新日期:1975-03-01 00:00:00
abstract::Bioassay-guided fractionation of a collection of Moorea bouillonii from Papua New Guinea led to the isolation of a new alkyl amide, mooreamide A (1), along with the cytotoxic apratoxins A-C and E. The planar structure of 1 was elucidated by NMR spectroscopy and mass spectrometry analysis. Structural homology between m...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3949-9
更新日期:2014-11-01 00:00:00
abstract::The all-cis-5,9,12-heptadecatrienoic acid was identified in the cellular slime mold Polysphondylium pallidum. The structural elucidation was accomplished by capillary gas chromatography, argentation thin-layer chromatography, and gas chromatography/mass spectrometry. This fatty acid has not been reported previously. ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02522934
更新日期:1996-04-01 00:00:00
abstract::Active lipases were isolated from the culture supernatant of the basidiomycetous fungus Pleurotus sapidus by foam fractionation. The pH value, the gas flow, and the foaming period were systematically varied to optimize the transport of the enzymes into the foam phase. On a 70-mL scale, a maximum recovery of hydrolytic...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-005-1389-x
更新日期:2005-03-01 00:00:00
abstract::The effect of essential fatty acid (EFA) deficiency on rat-brain myelin proteins was studied. Rats were maintained on a lipid-free diet and compared with control rats fed the same diet supplemented with 3% corn oil. At 17 days of age, each pup was injected with [3H]leucine and rats from each group were killed over a p...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537414
更新日期:1984-06-01 00:00:00
abstract::Cholesterol itself has very few structural/chemical features suitable for real-time imaging in living cells. Thus, the advent of dehydroergosterol [ergosta-5,7,9(11),22-tetraen-3beta-ol, DHE] the fluorescent sterol most structurally and functionally similar to cholesterol to date, has proven to be a major asset for re...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-008-3194-1
更新日期:2008-12-01 00:00:00
abstract::Intact washed human platelets aggregated in response to paf-acether (paf) and did not metabolize [3H]paf at concentrations up to 10 nM. However, when platelets were lysed by exposure to pH 9.5, resulting in 37.5 +/- 2.5% (mean +/- SD, n = 3) lactic dehydrogenase (LDH) release, 20.5 +/- 5.7% of the radioactivity was de...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536639
更新日期:1993-03-01 00:00:00
abstract::Minimal deviation hepatoma 7288 C cells were cultured in Swim's medium containing 10% serum for 48 hr. The growth medium was replaced with serum free media containing different concentrations of [1-14C] eicosa-8,11,14-trienoic acid and the cells were incubated for 24 hr. Incorporation into cell lipids, oxidation to CO...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532312
更新日期:1975-12-01 00:00:00
abstract::Screening for resistance to fenpropimorph was undertaken in order to isolate yeast mutants affected in the regulation of the ergosterol pathway. Among the mutants isolated, one bearing the recessive fen1-1 mutation was characterized by a 1.5-fold increase in the ergosterol level and a general resistance to sterol bios...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537499
更新日期:1993-10-01 00:00:00
abstract::This study proposed lipid fingerprinting of human seminal plasma by mass spectrometry as an analytical method to differentiate biological conditions. For this purpose, we chose infertile men as a model to study specific conditions, namely: high and low seminal plasma lipid peroxidation levels (sub-study 1.1), high and...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3922-7
更新日期:2014-09-01 00:00:00
abstract::A method has been developed for determination of individual long-chain fatty acyl-CoA esters from heart and skeletal muscle using high performance liquid chromatography (HPLC). The esters were extracted from freeze-clamped tissue of pig and rat hearts and rat skeletal muscle for analysis on a radially compressed C18 5...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535525
更新日期:1988-05-01 00:00:00
abstract::The biosynthesis of 3R-hydroxy-5Z, 8Z, 11Z,14Z-eicosatetraenoic acid (3R-HETE) from arachidonic acid (20:4n-6) by the hyphal-forming yeast, Dipodascopsis uninucleata, in cell-free enzyme extracts required CoASH, ATP, NAD+ and Mg2+; 3R-HETE was present as the CoA derivative in enzyme extracts and its biosynthesis was a...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-000-0637-4
更新日期:2000-11-01 00:00:00
abstract::Factors involved in reduced glutathione (GSH) and vitamin E-mediated inhibition of NADPH-dependent rat liver microsomal lipid peroxidation were examined. Lipid peroxidation was monitored over a time-course of 180 min by thiobarbituric acid reactive product formation. The addition of 5 mM GSH to the reaction system con...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544533
更新日期:1989-11-01 00:00:00
abstract::Omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA) are essential components of the diet of all vertebrates. The major dietary source of n-3 LC-PUFA for humans has been fish and seafood but, paradoxically, farmed fish are also reliant on marine fisheries for fish meal and fish oil (FO), traditionally major ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-016-4191-4
更新日期:2016-10-01 00:00:00
abstract::A fast and efficient shotgun lipidomics strategy was applied to analyze phospholipids (PL) in the oyster Crassostrea plicatula, including 29 species of phosphatidylcholine (PtdCho), 23 species of phosphatidylethanolamine (PtdEtn), 11 species of phosphatidylserine (PtdSer), 6 species of phosphatidylinositol (PtdIns), a...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-017-4305-7
更新日期:2017-12-01 00:00:00
abstract::A novel phospholipid containing a chromanol structure at its polar head group was synthesized from egg yolk phosphatidylcholine and 2,5,7,8-tetramethyl-6-hydroxy-2-(hydroxyethyl)chroman by transphosphatidylation catalyzed by phospholipase D from Streptomyces lydicus. The structure of the product synthesized was shown ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537147
更新日期:1994-02-01 00:00:00
abstract::The proximate and fatty acid compositions of the commercially important fish species (Engraulis encrasicolus, Alosa alosa, Belone belone, Scorpaena porcus, Pomatomus saltatrix, Mullus barbatus) from the Sinop region of the Black Sea were examined. The fat contents ranged from 1.26% (for scorpion fish) to 18.12% (for s...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-012-3658-1
更新日期:2012-06-01 00:00:00
abstract::Bile acids solubilize proteins from liver plasma membrane both in vivo and in vitro. The ability to solubilize the proteins is dependent on the species of bile acid. In this paper, the effect of phospholipid on the solubilization of a membrane-bound enzyme by bile acids was investigated in vitro. Taurocholate (TC) and...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534873
更新日期:1987-01-01 00:00:00
abstract::The aim of this study was to determine the influence of long-term docosahexaenoic acid (DHA) dietary supplementation on the erythrocyte fatty acid profile and oxidative balance in soccer players after training and acute exercise. Fifteen volunteer male athletes (age 20.0 ± 0.5 years) were randomly assigned to a placeb...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3976-6
更新日期:2015-02-01 00:00:00
abstract::This work reports the effect of the apolipoproteins A-I and A-II (apoA-I and apoA-II) on the release of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-8, and IL-1 receptor antagonist (IL-1Ra) and on the oxidative burst of human neutrophils. By themselves, apoA-I and apoA-II do not affect the basal liber...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0981-4
更新日期:2002-09-01 00:00:00
abstract::This review explores the effects of dietary long chain polyunsaturated fatty acids (LCPUFA) on various aspects of early immune development and their potential role in the development or the prevention of immune disease. Modern diets have become increasingly rich in n-6 LCPUFA and relatively n-3 LCPUFA deficient. These...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-007-3030-z
更新日期:2007-09-01 00:00:00
abstract::The uptake of lipid from the yolk by the yolk sac membrane of the chick embryo is accompanied by the rapid esterification of a large proportion of the yolk cholesterol. This could arise from enhanced acyl-CoA:cholesterol acyltransferase (ACAT) activity and/or inhibition of cholesteryl ester hydrolase (CEH) activity. T...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536056
更新日期:1993-07-01 00:00:00
abstract::Using an experimental model that enables the effects of alcohol to be distinguished from the effects of the nonalcoholic components present in wine, we determined whether wine has effects other than those of alcohol on the metabolism of cholesterol. Male rats were fed a standard diet and had free access to water and e...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0732-6
更新日期:2001-04-01 00:00:00
abstract::Reproductive tissues from male house and field crickets were investigated for the presence of arachidonic acid. Arachidonic acid was identified by retention behavior on 3 gas liquid chromatographic column systems-SE-30, OV 225, and Silar 10C-and by gas liquid/positive ion chemical ionization mass spectrometry. Arachid...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534928
更新日期:1981-01-01 00:00:00
abstract::Enrichment of beef muscle with n-3 fatty acids (FA) is one means to introduce these FA into the diet, but ruminal biohydrogenation limits their bioavailability. To address this problem, we evaluated the ability of condensed tannin (quebracho), in the presence or absence of casein, to protect 18:3n-3 in flaxseed from h...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-007-3126-5
更新日期:2007-12-01 00:00:00
abstract::In this study, to obtain further information on the structural diversity of non-methylene-interrupted (NMI) fatty acids (FA) in the gonads of the limpet Cellana toreuma, previously unreported minor NMI FA, fractionated by argentation thin-layer chromatography (TLC), in the ovaries were characterized by gas chromatogra...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12225
更新日期:2020-05-01 00:00:00
abstract::Trans FA (TFA) have at least one trans double bond and comprise several isomers and types, including many of the CLA (e.g., c9,t11-18:2 CLA). Some TFA may have adverse effects (e.g., cardiovascular disease), whereas some are thought to have beneficial effects (e.g., anticarcinogenicity). The presence of TFA in human t...
journal_title:Lipids
pub_type: 杂志文章,随机对照试验
doi:10.1007/s11745-006-5082-x
更新日期:2006-02-01 00:00:00
abstract::A method for the simultaneous quantitative analysis of prostaglandin E2 (PGE2) and PGE3 is described. The PG were analyzed by selected ion monitoring as the methyl ester-TMS ether derivatives of PGB2 and PGB3, respectively. The internal standard for the quantification of both species was [3,3,4,4-2H4]PGE2. A linear re...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535360
更新日期:1982-11-01 00:00:00
abstract::For four weeks, groups of eight male and eight female F344/N rats were fed diets containing 15.5, 20, 30 or 40% of energy (en%) as fat. The fat was composed of corn oil and beef tallow with 9 en% from linoleate in all diets. Females had greater mean hepatic alpha-tocopherol levels, whereas males had greater plasma alp...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535861
更新日期:1992-11-01 00:00:00
abstract::In the present study, changes in phospholipid compositions of liver microsomes, erythrocyte membranes, platelets, aorta, cardiac muscle and brain of rats fed olive oil were compared with those of rats fed sunflower oil. Four groups of rats starting at weaning were fed for four weeks a basal diet containing 5 or 25% ol...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536252
更新日期:1994-12-01 00:00:00