Foam fractionation of exo-lipases from a growing fungus (Pleurotus sapidus).

Abstract:

:Active lipases were isolated from the culture supernatant of the basidiomycetous fungus Pleurotus sapidus by foam fractionation. The pH value, the gas flow, and the foaming period were systematically varied to optimize the transport of the enzymes into the foam phase. On a 70-mL scale, a maximum recovery of hydrolytic activity of 95% was obtained at pH 7.0 and 60 mL N2 min(-1) after 50 min. The procedure, with minor modifications, was also applicable to native pellet cultures of P. sapidus. The same recovery of 95% was achieved, with purification and enrichment factors of 11.6 and 28.0, respectively.

journal_name

Lipids

journal_title

Lipids

authors

Linke D,Zorn H,Gerken B,Parlar H,Berger RG

doi

10.1007/s11745-005-1389-x

subject

Has Abstract

pub_date

2005-03-01 00:00:00

pages

323-7

issue

3

eissn

0024-4201

issn

1558-9307

journal_volume

40

pub_type

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