Abstract:
:Active lipases were isolated from the culture supernatant of the basidiomycetous fungus Pleurotus sapidus by foam fractionation. The pH value, the gas flow, and the foaming period were systematically varied to optimize the transport of the enzymes into the foam phase. On a 70-mL scale, a maximum recovery of hydrolytic activity of 95% was obtained at pH 7.0 and 60 mL N2 min(-1) after 50 min. The procedure, with minor modifications, was also applicable to native pellet cultures of P. sapidus. The same recovery of 95% was achieved, with purification and enrichment factors of 11.6 and 28.0, respectively.
journal_name
Lipidsjournal_title
Lipidsauthors
Linke D,Zorn H,Gerken B,Parlar H,Berger RGdoi
10.1007/s11745-005-1389-xsubject
Has Abstractpub_date
2005-03-01 00:00:00pages
323-7issue
3eissn
0024-4201issn
1558-9307journal_volume
40pub_type
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