Quantitative measurement of prostaglandins E2 and E2 by selected ion monitoring.

Abstract:

:A method for the simultaneous quantitative analysis of prostaglandin E2 (PGE2) and PGE3 is described. The PG were analyzed by selected ion monitoring as the methyl ester-TMS ether derivatives of PGB2 and PGB3, respectively. The internal standard for the quantification of both species was [3,3,4,4-2H4]PGE2. A linear response over the range 0.6--50 ng (1.7--143 pmoles) was demonstrated for PGE3. The chromatographic conditions used (2% SP-2330 column) afforded nearly baseline separation of the prostaglandins. New standard curves for PGE3 must be developed each time the ion source parameters are changed. In a typical calibration run, the instrumental precision, expressed as coefficient of variation, ranged from 1.1 to 7.2% for PGE2 (3 to 100 ng injected) and from 1.6 to 11.1% for PGE3 (1.5--50 ng injected). The method was applied to the PG analysis of rat renomedullary tissues. The recovery of synthetic PGE2 added to medullary homogenates was 100.5 +/- 1.7% (mean +/- SEM, n = 9), and the recovery of PGE3 was 91.3 +/- 1.4% (n = 9).

journal_name

Lipids

journal_title

Lipids

authors

Ferretti A,Flanagan VP,Roman JM

doi

10.1007/BF02535360

subject

Has Abstract

pub_date

1982-11-01 00:00:00

pages

825-30

issue

11

eissn

0024-4201

issn

1558-9307

journal_volume

17

pub_type

杂志文章

相关文献

LIPIDS文献大全
  • Lecithin-cholesterol acyltransferase: inhibition by local anesthetics in plasma from man and experimental animals in vitro.

    abstract::Lecithin-cholesterol acyltransferase (LCAT, EC 2.3.1.43) was assayed in vitro in plasma from normal man, rat, rabbit and dog by following the formation of 14C-cholesteryl esters subsequent to labeling the plasma with 14C-cholesterol in vitro. In all species examined, various local anesthetics were found to inhibit LCA...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534370

    authors: Bell FP,Hubert EV

    更新日期:1980-10-01 00:00:00

  • Thealpha-oxidation system of brain microsomes. Cofactors foralpha-hydroxy acid decarboxylation.

    abstract::A one-carbon degradation of long-chain fatty acids, which was found to occur in the brains of rats in vivo, has been investigated in a brain microsomal fraction in vitro. Decarboxylation of the alpha-hydroxy acid, a possible intermediate product between the substrate and the next shorter acid, in the presence of brain...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02531201

    authors: Macdonald RC,Mead JF

    更新日期:1968-05-01 00:00:00

  • Comparison of the effects of dietary alpha-linolenic, stearidonic, and eicosapentaenoic acids on production of inflammatory mediators in mice.

    abstract::The effects of dietary stearidonic acid (18:4n-3) on inflammatory mediator release in whole blood and splenocytes was investigated in Balb/c mice, and the effects were compared with those of two other n-3 PUFA: alpha-linolenic acid (18:3n-3) and EPA (20:5n-3). TAG mixtures containing 10% of 18:4n-3, 18:3n-3, or 20:5n-...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-002-0921-3

    authors: Ishihara K,Komatsu W,Saito H,Shinohara K

    更新日期:2002-05-01 00:00:00

  • Analysis of methylsterol fractions from twenty vegetable oils.

    abstract::The 4-monomethylsterol and 4,4-dimethylsterol fractions were separated from the unsaponifiables of 20 vegetable oils by preparative thin layer chromatography, and their compositions were determined by gas liquid chromatography. Tentative identification of the individual components of these fractions was carried out by...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02532729

    authors: Jeong TM,Itoh T,Tamura T,Matsumoto T

    更新日期:1975-10-01 00:00:00

  • Influence of diet on the kinetic behavior of hepatic carnitine palmitoyltransferase I toward different acyl CoA esters.

    abstract::The influence of diet on the kinetics of the overt form of rat liver mitochondrial carnitine palmitoyltransferase (CPT I; EC 2.3.1.21) was studied using rats fed either a low-fat diet (3% w/w fat), or diets which were supplemented with either olive oil (OO), safflower oil (SO) or menhaden (fish) oil (MO) to 20% w/w of...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-997-0005-4

    authors: Power GW,Cake MH,Newsholme EA

    更新日期:1997-01-01 00:00:00

  • Positional specificity of trans fatty acids in fetal lecithin.

    abstract::Differences in the positional incorporation of 9-trans[1-(14)C] octadecenoic (elaidic) and 9-trans,12-trans[1-(14)C] octadecadienoic (linoelaidic) acids in fetal lecithin of rats were demonstrated. On the 20th day of gestation, a 14C-labeled albumin complex of elaidic or linoelaidic acid was injected into the jugular ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535045

    authors: Moore CE,Dhopeshwarkar GA

    更新日期:1981-07-01 00:00:00

  • Lipid and fatty acid compositions of a novel docosahexaenoic acid-producing marine bacterium.

    abstract::An unidentified bacterial strain, SCRC-21406, isolated from the intestine of a marine fish, Glossanodon semifasciatus, produced docosahexaenoic acid at 23% (mol/mol) [= 28% (w/w)] of total fatty acids in a medium containing 0.5% (wt/vol) peptone and 0.1% (wt/vol) yeast extract at 12 degrees C under atmospheric pressur...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-997-0127-8

    authors: Watanabe K,Ishikawa C,Ohtsuka I,Kamata M,Tomita M,Yazawa K,Muramatsu H

    更新日期:1997-09-01 00:00:00

  • The simultaneous separation and quantitation of human milk lipids.

    abstract::A protocol using a dry column method was modified for the extraction of total lipids and the simultaneous separation and quantitation of neutral and polar lipids in human milk. The triacylglycerol, cholesterol, phospholipid and vitamin E contents of the lipid extracts were determined and compared with lipids extracted...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535216

    authors: Collins SE,Jackson MB,Lammi-Keefe CJ,Jensen RG

    更新日期:1989-08-01 00:00:00

  • Prenatal fatty acid status and immune development: the pathways and the evidence.

    abstract::This review explores the effects of dietary long chain polyunsaturated fatty acids (LCPUFA) on various aspects of early immune development and their potential role in the development or the prevention of immune disease. Modern diets have become increasingly rich in n-6 LCPUFA and relatively n-3 LCPUFA deficient. These...

    journal_title:Lipids

    pub_type: 杂志文章,评审

    doi:10.1007/s11745-007-3030-z

    authors: Prescott SL,Dunstan JA

    更新日期:2007-09-01 00:00:00

  • Diacylglycerol in Cationic Nanoparticles Stimulates Oxidative Stress-Mediated Death of Cancer Cells.

    abstract::Previous reports have suggested that cargo-free cationic nanoparticles (cNP) consisting of cationic monovalent lipids, such as 1,2-oleoyl-3-trimethylammonium-propane (DOTAP), induce reactive oxygen species (ROS) generation and toxicity in cells. In addition, cNP containing six lysine residues (6K) and cargo (6K-cNP) e...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1002/lipd.12124

    authors: Bae CS,Ahn T

    更新日期:2018-11-01 00:00:00

  • Supplementation with evening primrose oil in atopic dermatitis: effect on fatty acids in neutrophils and epidermis.

    abstract::We investigated the effect of oral supplementation with evening primrose oil, containing 72% linoleic acid (18:2n-6) and 10% gamma-linolenic acid (18:3n-6), on the epidermal and neutrophil phospholipid fatty acid composition in 15 patients with atopic dermatitis (AD). Three different dose levels, 4, 8 and 12 capsules ...

    journal_title:Lipids

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1007/BF02536604

    authors: Schäfer L,Kragballe K

    更新日期:1991-07-01 00:00:00

  • Hempseed Products Fed to Hens Effectively Increased n-3 Polyunsaturated Fatty Acids in Total Lipids, Triacylglycerol and Phospholipid of Egg Yolk.

    abstract::Hempseed products represent potential alternative feed ingredients for poultry. However, their usage is not currently approved due to a lack of data to support their safety and efficacy. In this regard, the current study was conducted to assess the impact of dietary concentration of hempseed (HS) products and duration...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-015-4088-7

    authors: Neijat M,Suh M,Neufeld J,House JD

    更新日期:2016-05-01 00:00:00

  • Quantitation of 1,2-diacylglycerol in rat heart by iatroscan TLC/FID.

    abstract::1,2-Diacylglycerol, which has been recognized as one of the intracellular second messengers, was measured quantitatively in the lipid extract from rat hearts using the thin layer chromatography and flame ionization detection (TLC/FID) method. Cholesterol acetate was added to the tissue as an internal standard, and the...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535467

    authors: Okumura K,Hashimoto H,Ito T,Ogawa K,Satake T

    更新日期:1988-03-01 00:00:00

  • Types of oilseed and adipose tissue influence the composition and relationships of polyunsaturated fatty acid biohydrogenation products in steers fed a grass hay diet.

    abstract::The current study evaluated the composition and relationships of polyunsaturated fatty acid biohydrogenation products (PUFA-BHP) from the perirenal (PRF) and subcutaneous fat (SCF) of yearling steers fed a 70 % grass hay diet with concentrates containing either sunflower-seed (SS) or flaxseed (FS). Analysis of varianc...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-013-3876-1

    authors: Mapiye C,Aalhus JL,Turner TD,Rolland DC,Basarab JA,Baron VS,McAllister TA,Block HC,Proctor SD,Dugan ME

    更新日期:2014-03-01 00:00:00

  • Effect of ingestion of thermally oxidized frying oil on peroxidative criteria in rats.

    abstract::Thermally oxidized rapeseed oils (4 levels of deterioration; used by a manufacturer of fried fish paste in a conventional manner) were fed to rats at a practical level of concentration. Rats were fed a diet ad libitum for 13 weeks that contained 15% of a test oil. The effects of the diet on several biochemical criteri...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534782

    authors: Izaki Y,Yoshikawa S,Uchiyama M

    更新日期:1984-05-01 00:00:00

  • Conjugated linoleic acids (CLA) as precursors of a distinct family of PUFA.

    abstract::One of the possibilities for distinct actions of c9,t11- and the t10,c12-conjugated linoleic acid (CLA) isomers may be at the level of metabolism since the conjugated diene structure gives to CLA isomers and their metabolites a distinct pattern of incorporation into the lipid fraction and metabolism. In fact, CLA appe...

    journal_title:Lipids

    pub_type: 杂志文章,评审

    doi:10.1007/s11745-004-1341-0

    authors: Banni S,Petroni A,Blasevich M,Carta G,Cordeddu L,Murru E,Melis MP,Mahon A,Belury MA

    更新日期:2004-11-01 00:00:00

  • Docosahexaenoic acid therapy in docosahexaenoic acid-deficient patients with disorders of peroxisomal biogenesis.

    abstract::A patient with classic Zellweger syndrome was treated with docosahexaenoic acid ethyl ester (DHA-EE) for three months. Five other patients with Zellweger variants (four of them less than one-year-old and a five-year-old) were treated with DHA-EE until normalization of the DHA levels in erythrocytes. When arachidonic a...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02637067

    authors: Martinez M

    更新日期:1996-03-01 00:00:00

  • cis-9,10-epoxystearic acid in human leukocytes: isolation and quantitative determination.

    abstract::Racemic cis-9,10-epoxystearic acid was isolated from total lipids of human leukocytes. Identification of the epoxy acid was based mainly on gas liquid chromatographic-mass spectrometric analysis and on its chemical conversion into threo-9,10-dihydroxystearic acid. A mass spectrometric method for quantitative determina...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535645

    authors: Fahlstadius P

    更新日期:1988-11-01 00:00:00

  • Docosapentaenoic acid does not completely replace DHA in n-3 FA-deficient rats during early development.

    abstract::The reciprocal replacement of DHA by docosapentaenoic acid (DPAn-6) was studied in rats that consumed an n-3 FA-deficient or n-3 FA-adequate diet. Dams were fed the two experimental diets from weaning and throughout pregnancy and lactation. Their pups were then fed the respective diets after weaning. Cortex FA analysi...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-003-1080-2

    authors: Greiner RS,Catalan JN,Moriguchi T,Salem N Jr

    更新日期:2003-04-01 00:00:00

  • The HMG-CoA reductase gene and lipid and lipoprotein levels: the multi-ethnic study of atherosclerosis.

    abstract::HMG-CoA reductase (HMGCR) is an enzyme involved in cholesterol synthesis. To investigate the contribution of the HMGCR gene to lipids and lipoprotein subfractions in different ethnicities, we performed an association study in the Multi-Ethnic Study of Atherosclerosis (MESA). In total, 2,444 MESA subjects [597 African-...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-009-3314-6

    authors: Chen YC,Chen YD,Li X,Post W,Herrington D,Polak JF,Rotter JI,Taylor KD

    更新日期:2009-08-01 00:00:00

  • Comparative study of lipogenic enzymes in several vertebrates.

    abstract::The liver lipogenic enzymes are compared among rats, chickens, frogs and fish. Although the apparent Km values of glucose-6-phosphate dehydrogenase for glucose-6-phosphate are not much different among all the species, those of malic enzyme for malate are much higher in chickens and fish than in rats and frogs. Glucose...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534511

    authors: Iritani N,Ikeda Y,Fukuda H,Katsurada A

    更新日期:1984-11-01 00:00:00

  • 1-14C-linoleic acid distribution in various tissue lipids of guinea pigs following an oral dose.

    abstract::A recent study on the metabolism of 1-14C-alpha-linolenic acid in the guinea pig revealed that the fur had the highest specific activity of all tissues examined, 48 h after dosing. The present study investigated the pattern of tissue lipid labeling following an oral dose of 1-14C-linoleic acid after the animals had be...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-001-0715-7

    authors: Fu Z,Attar-Bashi NM,Sinclair AJ

    更新日期:2001-03-01 00:00:00

  • Sulfur-substituted and alpha-methylated fatty acids as peroxisome proliferator-activated receptor activators.

    abstract::FA with varying chain lengths and an alpha-methyl group and/or a sulfur in the beta-position were tested as peroxisome proliferator-activated receptor (PPAR)alpha, -delta(beta), and -gamma ligands by transient transfection in COS-1 cells using chimeric receptor expression plasmids, containing cDNAs encoding the ligand...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-005-1359-3

    authors: Larsen LN,Granlund L,Holmeide AK,Skattebøl L,Nebb HI,Bremer J

    更新日期:2005-01-01 00:00:00

  • Oxidized cholesterol modulates age-related change in lipid metabolism in rats.

    abstract::For three weeks, male Sprague-Dawley rats at either four weeks (young) or eight months (adult) of age were pair-fed one of the purified diets free of or containing either 0.2% of oxidized cholesterol mixture (cholesterol oxidation products) or 0.2% of cholesterol. Although the food intake was similar, dietary oxidized...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02536298

    authors: Osada K,Kodama T,Noda S,Yamada K,Sugano M

    更新日期:1995-05-01 00:00:00

  • Mechanism of anesthesia: the potency of four derivatives of octane corresponds to their hydrogen bonding capacity.

    abstract::The anesthetic potency of four derivatives of n-octane was measured by tadpole righting reflex and expressed as effective millimolar concentration of drug in membrane, EDM50. Potency diminished (ED50 increased) in this order: 1-octanol, EDM50 = 5.5; 1-(2-methoxyethoxy)octane, EDM50 = 28; 1-methoxyoctane, EDM50 = 61; a...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534936

    authors: Brockerhoff H,Brockerhoff S,Box LL

    更新日期:1986-06-01 00:00:00

  • Dose-response impact of various tocotrienols on serum lipid parameters in 5-week-old female chickens.

    abstract::The cholesterol-suppressive action of the tocotrienol-rich-fraction (TRF) of palm oil may be due to the effect of its constituent tocotrienols on beta-hydroxy-beta-methylglutaryl coenzyme A (HMG-CoA) reductase activity. The tocotrienols, modulate HMG-CoA reductase activity via a post-transcriptional mechanism. As a co...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-006-5119-1

    authors: Yu SG,Thomas AM,Gapor A,Tan B,Qureshi N,Qureshi AA

    更新日期:2006-05-01 00:00:00

  • The relative incorporation of arachidonic and eicosapentaenoic acids into human platelet phospholipids.

    abstract::The incorporation of arachidonic acid (AA) as compared to eicosapentaenoic acid (EPA) into human platelet phospholipids was tested by incubating washed platelets with a known mixture of [3H]AA and [14C]EPA. Following incubation, the platelet lipids were extracted, the individual phospholipids--phosphatidylcholine (PC)...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534401

    authors: Weaver BJ,Holub BJ

    更新日期:1985-11-01 00:00:00

  • Dietary alpha-linolenic acid alters tissue fatty acid composition, but not blood lipids, lipoproteins or coagulation status in humans.

    abstract::We examined the effect of dietary alpha-linolenic acid (ALA) on the indices of lipid and coagulation status and on the fatty acid composition of serum and peripheral blood mononuclear cell (PBMNC) lipids in ten healthy men (age 21-37 yr) who consumed all their meals at the Western Human Nutrition Research Center for 1...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02536085

    authors: Kelley DS,Nelson GJ,Love JE,Branch LB,Taylor PC,Schmidt PC,Mackey BE,Iacono JM

    更新日期:1993-06-01 00:00:00

  • Involvement of phospholipids in apolipoprotein B modification during low density lipoprotein oxidation.

    abstract::An increased amount of phospholipids remained attached on delipidated apolipoprotein B originated from oxidized low density lipoprotein (LDL). 31P nuclear magnetic resonance analysis of such apolipoprotein showed an organic phosphorus peak at -0.55 ppm, which suggests the formation of adducts (most probably Schiff bas...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-998-0318-3

    authors: Karakatsani AI,Liapikos TA,Troganis AN,Tsoukatos DC

    更新日期:1998-12-01 00:00:00

  • The metabolism of dihomo-gamma-linolenic acid in man.

    abstract::Orally administered dihomo-gamma-linolenic acid (DHLA) is well absorbed in man; it appears in blood after ca. 4 hr first as triglyceride ester and later as phospholipid. After sustained-dosing, DHLA penetrated membrane pools and all phospholipid components but, depending on the dosage, reached a metabolic equilibrium ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533869

    authors: Stone KJ,Willis AL,Hart WM,Kirtland SJ,Kernoff PB,McNicol GP

    更新日期:1979-02-01 00:00:00