Abstract:
:A protocol using a dry column method was modified for the extraction of total lipids and the simultaneous separation and quantitation of neutral and polar lipids in human milk. The triacylglycerol, cholesterol, phospholipid and vitamin E contents of the lipid extracts were determined and compared with lipids extracted using a modified Folch procedure. Good precision for the extraction of neutral, polar and total lipids, as well as the different lipid classes, was demonstrated. No significant differences were found between the two methods with respect to the amount of cholesterol, phospholipid, total lipid or vitamin E extracted, thus validating the method as an extraction technique. We discuss the relationship between vitamin E and the three major milk lipids as an indicator of the vitamin's place of origin in the mammary gland. Our findings do not support the idea that vitamin E in mature milk has its original location in the apical membrane.
journal_name
Lipidsjournal_title
Lipidsauthors
Collins SE,Jackson MB,Lammi-Keefe CJ,Jensen RGdoi
10.1007/BF02535216subject
Has Abstractpub_date
1989-08-01 00:00:00pages
746-9issue
8eissn
0024-4201issn
1558-9307journal_volume
24pub_type
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