The simultaneous separation and quantitation of human milk lipids.

Abstract:

:A protocol using a dry column method was modified for the extraction of total lipids and the simultaneous separation and quantitation of neutral and polar lipids in human milk. The triacylglycerol, cholesterol, phospholipid and vitamin E contents of the lipid extracts were determined and compared with lipids extracted using a modified Folch procedure. Good precision for the extraction of neutral, polar and total lipids, as well as the different lipid classes, was demonstrated. No significant differences were found between the two methods with respect to the amount of cholesterol, phospholipid, total lipid or vitamin E extracted, thus validating the method as an extraction technique. We discuss the relationship between vitamin E and the three major milk lipids as an indicator of the vitamin's place of origin in the mammary gland. Our findings do not support the idea that vitamin E in mature milk has its original location in the apical membrane.

journal_name

Lipids

journal_title

Lipids

authors

Collins SE,Jackson MB,Lammi-Keefe CJ,Jensen RG

doi

10.1007/BF02535216

subject

Has Abstract

pub_date

1989-08-01 00:00:00

pages

746-9

issue

8

eissn

0024-4201

issn

1558-9307

journal_volume

24

pub_type

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