Abstract:
:Bile acid contents were determined for isolated rat hepatocytes. During the course of isolating the hepatocytes, perfusion of rat liver with buffer containing 2% albumin eluted a significant amount of bile acids. The elution was proportional to the volume of the buffer and attributable to albumin in the buffer. The isolated hepatocytes prepared by perfusion with 0.1% albumin buffer, which eluted a negligible amount of bile acids, contained 95 +/- 12 micrograms/10(8) cells of bile acids. The major bile acids were cholic acid (22%), beta-muricholic acid (34%) and hyodeoxycholic acid (10%). Levels of the other bile acids were less than 3%. Peak 8, unidentified but presumed to be a trihydroxycholanoic acid, accounted for 19%.
journal_name
Lipidsjournal_title
Lipidsauthors
Hashimoto S,Uchida K,Hirata Mdoi
10.1007/BF02535094subject
Has Abstractpub_date
1982-03-01 00:00:00pages
149-54issue
3eissn
0024-4201issn
1558-9307journal_volume
17pub_type
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