Hepatic bile acid elution by albumin and bile acid content in isolated rat hepatocytes.

Abstract:

:Bile acid contents were determined for isolated rat hepatocytes. During the course of isolating the hepatocytes, perfusion of rat liver with buffer containing 2% albumin eluted a significant amount of bile acids. The elution was proportional to the volume of the buffer and attributable to albumin in the buffer. The isolated hepatocytes prepared by perfusion with 0.1% albumin buffer, which eluted a negligible amount of bile acids, contained 95 +/- 12 micrograms/10(8) cells of bile acids. The major bile acids were cholic acid (22%), beta-muricholic acid (34%) and hyodeoxycholic acid (10%). Levels of the other bile acids were less than 3%. Peak 8, unidentified but presumed to be a trihydroxycholanoic acid, accounted for 19%.

journal_name

Lipids

journal_title

Lipids

authors

Hashimoto S,Uchida K,Hirata M

doi

10.1007/BF02535094

subject

Has Abstract

pub_date

1982-03-01 00:00:00

pages

149-54

issue

3

eissn

0024-4201

issn

1558-9307

journal_volume

17

pub_type

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