Decrease in alkaline triglyceride lipase in primary cultured hepatocytes from mice with sarcoma 180.

Abstract:

:Primary cultured hepatocytes from normal mice and mice with Sarcoma 180 were characterized. The viability of freshly isolated hepatocytes from both sources was over 90% and the cells had a relatively stable population of DNA for a minimum of three days. After incubation with (3H)leucine, the syntheses and secretions of (3H)labeled trichloracetic acid-insoluble materials by hepatocytes from both normal and tumor-bearing mice increased similarly. However, the alkaline triglyceride lipase activity of a homogenate of freshly isolated hepatocytes from tumor-bearing mice was one-third that of cells from normal mice. The activity of hepatocytes from tumor-bearing mice increased less during culture than did the activity of cells isolated from normal mice.

journal_name

Lipids

journal_title

Lipids

authors

Masuno H,Okuda H

doi

10.1007/BF02534229

subject

Has Abstract

pub_date

1985-07-01 00:00:00

pages

399-403

issue

7

eissn

0024-4201

issn

1558-9307

journal_volume

20

pub_type

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