Abstract:
:Fast atom bombardment-tandem mass spectrometry was used to identify molecular species of paf-acether (paf) produced by human polymorphonuclear neutrophils. Using this biological material, normal phase high performance liquid chromatography was necessary prior to the fast atom bombardment-tandem mass spectrometry step. Gas liquid chromatography/electron capture detection after hydrolysis with phospholipase C and conversion to heptafluorobutyrate derivatives was used to confirm the results. The results indicated the presence of mainly 1-O-hexadecyl/octadecyl-2-acetyl-sn-glycero-3-phosphocholine, acyl analogs of paf and only trace amounts of other alkyl analogs of paf. We did not detect the 2-propionyl analog of paf. Moreover, supplementation of human polymorphonuclear neutrophils with sodium propionate did not result in formation of the 2-propionyl analog of paf.
journal_name
Lipidsjournal_title
Lipidsauthors
Bossant MJ,Farinotti R,De Maack F,Mahuzier G,Benveniste J,Ninio Edoi
10.1007/BF02535248subject
Has Abstractpub_date
1989-02-01 00:00:00pages
121-4issue
2eissn
0024-4201issn
1558-9307journal_volume
24pub_type
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