Abstract:
:To address the role of cell membrane neutral sphingomyelinase (EC 3.1.4.12; SMase) in the regulation of cholesterol metabolism in the liver parenchymal cell, we examined the effect of exogenous neutral SMase on the metabolism of cholesteryl esters and the secretion of VLDL and biliary lipids in isolated rat hepatocytes. We show that treatment of hepatocytes with SMase (20 mU/mL) resulted in the intracellular buildup of cholesteryl esters, increased ACAT (EC 2.3.1.26) activity without affecting the ACAT2 mRNA level, and increased cytosolic and microsomal cholesteryl ester hydrolase (EC 3.1.1.13) activity. This was accompanied by increases in the secretion of biliary bile acid, phospholipid, and cholesterol and in increased cholesterol 7alpha-hydroxylase (EC 1.14.13.17) activity and levels of mRNA, as well as decreased levels of apoB mRNA and a decreased secretion of VLDL apoB (apoB-48, approximately 45%; apoB-100, approximately 32%) and lipids (approximately 55%). Moreover, the VLDL particles secreted had an abnormal size and lipid composition; they were larger than controls, were relatively enriched in cholesteryl ester, and depleted in TG and cholesterol. Cell-permeable ceramides did not replicate any of the reported effects. These findings demonstrate that the increased cholesteryl ester turnover, oversecretion of biliary cholesterol and bile acids, and undersecretion of VLDL cholesterol and particles are concerted responses of the primary hepatocytes to exogenous neutral SMase brought about by regulation at several levels. We suggest that plasma membrane neutral SMase may have a specific, ceramide-independent effect in the regulation of cholesterol output pathways in hepatocytes.
journal_name
Lipidsjournal_title
Lipidsauthors
Liza M,Chico Y,Fresnedo O,Ochoa Bdoi
10.1007/s11745-003-1031-ysubject
Has Abstractpub_date
2003-01-01 00:00:00pages
53-63issue
1eissn
0024-4201issn
1558-9307journal_volume
38pub_type
杂志文章相关文献
LIPIDS文献大全abstract::Plaques and white matter from brains of multiple sclerosis (MS) patients were analyzed for lipid content, class composition, and fatty acid composition of total lipid, together with the fatty acid composition of plaque glycerophospholipids, and the results were compared with white matter from normal brain. Plaques con...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544017
更新日期:1991-01-01 00:00:00
abstract::Yeast mutant strains auxotrophic for ergosterol and blocked in mevalonate diphosphate decarboxylase (erg19) and farnesyl diphosphate (FPP) synthetase (erg20) were isolated. The main feature of the mutants blocked in FPP synthetase is their ability to excrete prenyl alcohols, such as geraniol and farnesol. The isolatio...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536428
更新日期:1991-08-01 00:00:00
abstract::After administering the equivalent of 1 g of eicosapentaenoic acid (EPA) in four different chemical forms, the kinetics of EPA incorporation into plasma triglycerides (TG) were compared by gas liquid chromatography on a capillary column following separation of the lipid fraction by thin layer chromatography. EPA incor...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533970
更新日期:1987-10-01 00:00:00
abstract::Some 1,2- and 1,3-diacyl glycerols (with acyl groups as stearyl, oleyl, linoleyl, or stearolyl) were synthesized by conventional methods. The diacyl glycerols were esterified with 6-bromo-hexanoic acid to give the corresponding bromo-triacylglycerols (of the type AAB and ABA containing a bromo group at the distal part...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0769-6
更新日期:2001-06-01 00:00:00
abstract::Dietary fat intake influences plasma glucose concentration through modifying glucose uptake and utilization by adipose and skeletal muscle tissues. In this paper, we studied the effects of a low-fat diet on diaphragm GLUT4 expression and fatty acid composition in control and streptozotocin-induced diabetic rats. Contr...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-999-0426-0
更新日期:1999-08-01 00:00:00
abstract::A 24-hr pretreatment of cultured human fibroblasts with trifluoperazine induced a marked increase in incorporation of saturated (stearic, palmitic) and unsaturated (oleic, arachidonic) fatty acids into phospholipids (1.5- to 2-fold for 5.10(-5) M trifluoperazine). Concomitantly, incorporation into cholesteryl esters w...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535513
更新日期:1988-05-01 00:00:00
abstract::FA ethyl esters (FAEE) are nonoxidative metabolites of ethanol produced by the esterification of FA and ethanol. FAEE have been implicated as mediators of ethanol-induced organ damage in vivo and in vitro, and are markers of ethanol intake. Upon ethanol intake, FAEE are synthesized in the liver and pancreas in signifi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-1160-3
更新日期:2003-10-01 00:00:00
abstract::By in vivo and in vitro studies of L-(3-3H)serine and [9,10(n)-3H]palmitic acid incorporation into phospholipids, we show a change in the renewal of the ceramide moiety of sphingomyelin in the gills of euryhaline fish (sea bass and eels) when the animals were subjected to abrupt alterations in environmental salinity. ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02522924
更新日期:1996-04-01 00:00:00
abstract::The effect of apo very low density lipoprotein (apo VLDL) and apoprotein C-II on the phospholipase A1 activity associated with lipoprotein lipase (E.C.3.1.1.3) was studied using purified bovine milk lipoprotein lipase. The enzyme degraded 14C phosphatidylcholine (PC) to 14C 2-acyl lysophosphatidylcholine at a rate of ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02540967
更新日期:1980-03-01 00:00:00
abstract::Several ions in the mass spectra of wax esters were related to the molecular structures. Assigned structures of ions were confirmed by deuterium labeling. A simple, direct method for quantititive analyses of mixtures was developed. The method involved a comparison of sets of three ions, RCO2H(+), RCO2H2 (+) and [R'-1]...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02531236
更新日期:1971-07-01 00:00:00
abstract::Naturally occurring tetraalkylsubstituted furan fatty acids (F-acids) were tested as potential substrates for soybean lipoxygenase-1. For this purpose, F-acid methyl ester and phosphatidylcholines containing F-acids at the sn-2 position of the glycerol residue were incubated with the enzyme. Oxidation of F-acids only ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537308
更新日期:1994-06-01 00:00:00
abstract::A sucrose-rich diet (SRD) induces insulin resistance and dyslipidemia with impaired hepatic glucose production and gluconeogenesis, accompanied by altered post-receptor insulin signaling steps. The aim of this study was to examine the effectiveness of fish oil (FO) to reverse or improve the impaired hepatic glucose me...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-011-3623-4
更新日期:2012-02-01 00:00:00
abstract::Many reports have shown the beneficial effects of consumption of pine seeds and pine seed oil. However, few studies have examined the biological effect of pinolenic acid (PNA; 5,9,12-18:3), the main fatty acid in pine seed oil. In this study, using murine macrophage RAW264.7 cells as a model, we examined the effect of...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-008-3276-0
更新日期:2009-03-01 00:00:00
abstract::Postprandial chylomicrons are potent ultimate acceptors of cell membrane cholesterol and are believed to accelerate reverse cholesterol transport (RCT). We compared the effects of meals rich in polyunsaturated fat (PUFA) and either high (605 mg) or low (151 mg) in cholesterol and a meal rich in dairy fat (DF) in the f...
journal_title:Lipids
pub_type: 杂志文章,随机对照试验
doi:10.1007/s11745-007-3101-1
更新日期:2007-10-01 00:00:00
abstract::For four weeks, groups of eight male and eight female F344/N rats were fed diets containing 15.5, 20, 30 or 40% of energy (en%) as fat. The fat was composed of corn oil and beef tallow with 9 en% from linoleate in all diets. Females had greater mean hepatic alpha-tocopherol levels, whereas males had greater plasma alp...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535861
更新日期:1992-11-01 00:00:00
abstract::We have studied the effect of various diets on the phospholipid fatty acid composition and in vitro delta 5 desaturase activity of hepatic microsomes derived either from the normal or streptozotocin-induced diabetic rat. The diets studied were the standard rat chow diet and a basal fat-free diet supplemented either wi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535763
更新日期:1989-10-01 00:00:00
abstract::Two unusual lipid classes were detected by thin-layer chromatography in the neutral lipids derived from goat cauda-epididymal sperm plasma membrane. The lipids were identified as wax esters and 1-O-alkyl-2,3-diacylglycerols based on chromatographic properties, identity of their hydrolysis products, and infrared/1H nuc...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537065
更新日期:1992-01-01 00:00:00
abstract::Left ventricular (LV) remodeling after myocardial infarction constitutes the structural basis for ventricular dysfunction and heart failure. The characterization underlying the expression of lipoprotein receptors in cardiac dysfunction is scarcely explored. The aim of this study was to analyze the status of lipoprotei...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12014
更新日期:2018-02-01 00:00:00
abstract::CLA inhibits mammary cancer and reduces body fat accumulation in rodents. It is not known whether uncoupling proteins (UCP), which are modulators of energy balance and metabolism, play a role in these actions of CLA. To determine the effects of dietary CLA on the expression of UCP in various tissues, 5-wk-old Sprague-...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0971-6
更新日期:2002-09-01 00:00:00
abstract::Fatty acid composition of plasma phospholipids (PL), triglycerides (TG), and sterol esters (STE) was determined by high-resolution capillary gas-liquid chromatography in 22 obese children (age: 13.7 +/- 1.4 y, body weight relative to normal weight for height: 170 +/- 24%, mean +/- SD) and compared with data obtained i...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02529877
更新日期:1996-03-01 00:00:00
abstract::The suppression of plasma very low density lipoprotein (VLDL) triglyceride levels by dietary fish oils rich in polyunsaturated n-3 fatty acids has been attributed to decreased hepatic VLDL secretion. To investigate the effect of n-3 fatty acids on lipid metabolism and VLDL secretion in a tissue culture system, we incu...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537160
更新日期:1990-09-01 00:00:00
abstract::Plasma and liver lipids were studied in male weanling rats fed diets containing moderate levels of fat (6% by weight) as sunflower oil (SF diet, rich in linoleic acid), salmon oil (SM diet, rich in long-chain n-3 fatty acids), or a blend of peanut and rapeseed oil (PR diet, rich in oleic acid). After nine weeks of fee...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02522983
更新日期:1996-08-01 00:00:00
abstract::Mice exposed to cold rely upon brown adipose tissue (BAT)-mediated nonshivering thermogenesis to generate body heat using dietary glucose and lipids from the liver and white adipose tissue. In this report, we investigate how cold exposure affects the PI3 K/Akt signaling cascade and the expression of genes involved in ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-010-3422-3
更新日期:2010-06-01 00:00:00
abstract::The objective of this study was to establish the impact of caloric restriction on high fat diet-induced alterations on regulators of skeletal muscle growth. We hypothesized that caloric restriction would reverse the negative effects of high fat diet-induced obesity on REDD1 and mTOR-related signaling. Following an ini...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-016-4168-3
更新日期:2016-08-01 00:00:00
abstract::The n-6 and n-3 fatty acid status of developing organs is the cumulative result of the diet lipid composition and many complex events of lipid metabolism. Little information is available, however, on the potential effects of the saturated fatty acid chain length (8:0-16:0) or oleic acid (18:1) content of the diet on t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535583
更新日期:1992-12-01 00:00:00
abstract::Among many factors, elevated lipids and lipid peroxide levels in blood are major risk factors in the development of cardiovascular disease in diabetic patients. This study has examined whether oral supplementation of vitamin E, an antioxidant, has any effect on blood lipid peroxidation products (LP) and lipid profile ...
journal_title:Lipids
pub_type: 临床试验,杂志文章
doi:10.1007/BF02637057
更新日期:1996-03-01 00:00:00
abstract::A new method of isoelectric focusing (IEF) and pH gradient electrophoresis, using thin layers of agarose gel beads, was devised to investigate chylomicrons and very low density lipoproteins (VLDL). pH gradient stability and cathodal gradient drift were similar to those of polyacrylamide gel IEF, and linearity of gradi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534130
更新日期:1979-10-01 00:00:00
abstract::A fluorescent radiolabeled triacylglycerol has been synthesized by using a fluorescent fatty acid (pyrene decanoic acid) and a radiolabeled oleic acid. This analog of the natural substrate, 1(3)pyrene decanoic-2,3 (1,2)-dioleoyl-sn-glycerol, has been tested as substrate for determining lipoprotein lipase and hepatic t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535605
更新日期:1988-06-01 00:00:00
abstract::Using primary rat hepatocyte cultures, the potency of several garlic-derived organosulfur compounds to inhibit cholesterol biosynthesis in toto as well as at early and late steps of this metabolic pathway was compared. Concerning early steps, allicin significantly inhibited incorporation of [14C]acetate into nonsaponi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02587912
更新日期:1996-12-01 00:00:00
abstract::This paper represents the first synthesis, spectroscopic characterization, and antitumor evaluation of F-, N-, and S-containing C4alpha-FA derivatives of podophyllotoxin. In a synthetic strategy, a FA unit of 4-O-podophyllotoxinyl 12-hydroxyoctadec-Z-9-enoate 2, a derivative of podophyllotoxin, was functionalized at t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-1397-x
更新日期:2005-04-01 00:00:00