Trifluoperazine increases fatty acid turnover in phospholipids in cultured human fibroblasts.

Abstract:

:A 24-hr pretreatment of cultured human fibroblasts with trifluoperazine induced a marked increase in incorporation of saturated (stearic, palmitic) and unsaturated (oleic, arachidonic) fatty acids into phospholipids (1.5- to 2-fold for 5.10(-5) M trifluoperazine). Concomitantly, incorporation into cholesteryl esters was strongly inhibited (20% of control for 5.10(-5) M trifluoperazine). The drug did not change the phospholipid composition of treated cells. The effect of trifluoperazine on oleic acid incorporation into phospholipids was time-dependent and reached a maximum after a six-hr preincubation with the drug. Trifluoperazine also induced an increase in the rate of chase of oleic acid from the different phospholipid classes. In vitro preincubation of cell-free extracts with trifluoperazine resulted in activation of phospholipid acyltransferases, whereas cholesterol acyltransferase activity was decreased. The rapid effect of trifluoperazine together with its effect on a cell-free system suggests a direct action of this amphiphilic drug on the acyltransferase activities, probably by modification of the structural organization of cellular membranes.

journal_name

Lipids

journal_title

Lipids

authors

Mazière C,Mazière JC,Mora L,Auclair M,Polonovski J

doi

10.1007/BF02535513

subject

Has Abstract

pub_date

1988-05-01 00:00:00

pages

419-23

issue

5

eissn

0024-4201

issn

1558-9307

journal_volume

23

pub_type

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