An improved method for the measurement of malondialdehyde in biological samples.

Abstract:

:An improved method was developed for measuring malondialdehyde (MDA) as its thiobarbituric acid (TBA) complex. Samples were initially incubated with 1% potassium iodide and 0.1% butylated hydroxytoluene at 50 degrees C for 20 min, and then with 0.4% TBA at 60 degrees C for 60 min. The MDA-TBA complex formed was extracted with isobutyl alcohol and measured by high-performance liquid chromatography with fluorescence detection. The improved method allows for a more specific determination of MDA present in biological samples.

journal_name

Lipids

journal_title

Lipids

authors

Li XY,Chow CK

doi

10.1007/BF02537094

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

73-5

issue

1

eissn

0024-4201

issn

1558-9307

journal_volume

29

pub_type

杂志文章

相关文献

LIPIDS文献大全
  • Concentration-dependent effects of eicosapentaenoic acid on very low density lipoprotein secretion by the isolated perfused rat liver.

    abstract::The effects of increasing concentrations of eicosapentaenoic acid (20:5n-3; EPA) and oleic acid (18:1n-9; OA) on esterification to triacylglycerols (TG) and phospholipids (PL), and the relationship to formation and secretion of the very low density lipoproteins (VLDL) were compared in the isolated perfused rat liver. ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535940

    authors: Zhang ZJ,Wilcox HG,Castellani L,Fungwe TV,Elam MB,Heimberg M

    更新日期:1993-05-01 00:00:00

  • A specific, sensitive and high-capacity immunoassay for PAF.

    abstract::A specific radioimmunoassay for platelet-activating factor (PAF) sensitive in the range 10-1000 pg (0.02-2 pmoles) has been developed. Detailed quantitative hapten inhibition studies showed specificity for the acetyl group at C-2 of PAF, a requirement for the ether linkage at C-1 and some tolerance for substituents on...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02536517

    authors: Baldo BA,Smal MA,McCaskill AC

    更新日期:1991-12-01 00:00:00

  • Triolein and trilinolein ameliorate oxidized low-density lipoprotein-induced oxidative stress in endothelial cells.

    abstract::Uptake of oxidized low-density lipoprotein by endothelial cells is a critical step for the initiation of atherosclerosis. Triacylglycerol uptake in these cells is understood to be a part of the process. The present investigation, comparison among the effects of simple acylglycerol, including tristearin, triolein, and ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-014-3889-4

    authors: Luo T,Deng ZY,Li XP,Rao H,Fan YW

    更新日期:2014-05-01 00:00:00

  • High Sucrose Intake Ameliorates the Accumulation of Hepatic Triacylglycerol Promoted by Restraint Stress in Young Rats.

    abstract::Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disorder. Stress promotes the onset of the NAFLD with a concomitant increment in the activity of the hepatic 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD-1). However, the interaction between the stress and a carbohydrate-enriched diet for t...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-015-4066-0

    authors: Corona-Pérez A,Díaz-Muñoz M,Rodríguez IS,Cuevas E,Martínez-Gómez M,Castelán F,Rodríguez-Antolín J,Nicolás-Toledo L

    更新日期:2015-11-01 00:00:00

  • (S)-1,2-diacyl-3-acetins: Optically active triglycerides fromEuonymus verrucosus seed oil.

    abstract::The seed oil ofEuonymus verrucosus Scop., family Celastraceae, contains more than 90% 1,2-diacyl-3-acetins (monoacetotriglycerides). The constituent triglyceride acids, other than acetic, are the usual long-chain fatty acids.Thin-layer chromatography (TLC), infrared (IR) and hydrolysis with pancreatic lipase indicate ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533174

    authors: Kleiman R,Miller RW,Earle FR,Wolff IA

    更新日期:1967-11-01 00:00:00

  • Effects of soybean lipoxygenase-1 on phosphatidylcholines containing furan fatty acids.

    abstract::Naturally occurring tetraalkylsubstituted furan fatty acids (F-acids) were tested as potential substrates for soybean lipoxygenase-1. For this purpose, F-acid methyl ester and phosphatidylcholines containing F-acids at the sn-2 position of the glycerol residue were incubated with the enzyme. Oxidation of F-acids only ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02537308

    authors: Batna A,Spiteller G

    更新日期:1994-06-01 00:00:00

  • The fatty acid 8,11-diol synthase of Aspergillus fumigatus is inhibited by imidazole derivatives and unrelated to PpoB.

    abstract::(8R)-Hydroperoxy-(9Z,12Z)-octadecadienoic acid (8-HPODE) is formed by aspergilli as an intermediate in biosynthesis of oxylipins with effects on sporulation. 8-HPODE is transformed by separate diol synthases to (5S,8R)-dihydroxy- and (8R,11S)-dihydroxy-(9Z,12Z)-octadecadienoic acids (5,8- and 8,11-DiHODE). The former ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-012-3673-2

    authors: Jernerén F,Oliw EH

    更新日期:2012-07-01 00:00:00

  • Comparison of lipid composition of Aedes aegypti and Aedes albopictus cells obtained from logarithmic and stationary phases of growth.

    abstract::The total lipids, total neutral lipids, and total phospholipids from Aedes aegypti and aedes albopictus cells cultivated in vitro in a medium containing fetal calf serum were analyzed. The mosquito cells were harvested in the logarithmic and stationary phases of growth. The fatty acid profiles of the lipids showed dif...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02532163

    authors: McMeans E,Yang TK,Anderson LE,Jenkin HM

    更新日期:1975-02-01 00:00:00

  • Essentiality of fatty acids.

    abstract::All fatty acids have important functions, but the term "essential" is applied only to those polyunsaturated fatty acids (PUFA) that are necessary for good health and cannot be completely synthesized in the body. The need for arachidonic acid, which is utilized for eicosanoid synthesis and is a constituent of membrane ...

    journal_title:Lipids

    pub_type: 杂志文章,评审

    doi:10.1007/BF02562220

    authors: Spector AA

    更新日期:1999-01-01 00:00:00

  • Polyunsaturated fatty acids inhibit hepatic stearoyl-CoA desaturase-1 gene in diabetic mice.

    abstract::Insulin and dietary fructose independently induce stearoyl-CoA desaturase 1 (SCD1) gene expression in diabetic mouse liver. In the present study, we again used diabetic mice and supplemented a high fructose diet with polyunsaturated fatty acids (PUFA) to determine the selective repression of SCD1 gene expression by di...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02637047

    authors: Waters KM,Ntambi JM

    更新日期:1996-03-01 00:00:00

  • Fluorescence techniques using dehydroergosterol to study cholesterol trafficking.

    abstract::Cholesterol itself has very few structural/chemical features suitable for real-time imaging in living cells. Thus, the advent of dehydroergosterol [ergosta-5,7,9(11),22-tetraen-3beta-ol, DHE] the fluorescent sterol most structurally and functionally similar to cholesterol to date, has proven to be a major asset for re...

    journal_title:Lipids

    pub_type: 杂志文章,评审

    doi:10.1007/s11745-008-3194-1

    authors: McIntosh AL,Atshaves BP,Huang H,Gallegos AM,Kier AB,Schroeder F

    更新日期:2008-12-01 00:00:00

  • sn-position determination of phospholipid-linked fatty acids derived from erythrocytes by liquid chromatography electrospray ionization ion-trap mass spectrometry.

    abstract::The sn-position of FA in membrane lipids has an influence on the physiological function of cells, is predictive for diseases, and therefore is useful for diagnostics. The current study compares the compositions of acyl chain substituents in the sn-1 and sn-2 positions of the glycerol backbones of phospholipids derived...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-005-1377-1

    authors: Beermann C,Möbius M,Winterling N,Schmitt JJ,Boehm G

    更新日期:2005-02-01 00:00:00

  • Analysis of methylsterol fractions from twenty vegetable oils.

    abstract::The 4-monomethylsterol and 4,4-dimethylsterol fractions were separated from the unsaponifiables of 20 vegetable oils by preparative thin layer chromatography, and their compositions were determined by gas liquid chromatography. Tentative identification of the individual components of these fractions was carried out by...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02532729

    authors: Jeong TM,Itoh T,Tamura T,Matsumoto T

    更新日期:1975-10-01 00:00:00

  • Identification of diacylglycerol acyltransferase inhibitors from Rosa centifolia petals.

    abstract::Diacylglycerol acyltransferase (DGAT) catalyzes the final step of triacylglycerol (TAG) synthesis, and is considered as a potential target to control hypertriglyceridemia or other metabolic disorders. In this study, we found that the extract of rose petals suppressed TAG synthesis in cultured cells, and that the extra...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-011-3559-8

    authors: Kondo H,Hashizume K,Shibuya Y,Hase T,Murase T

    更新日期:2011-08-01 00:00:00

  • Tocopherol-phospholipid liposomes: maximum content and stability to serum proteins.

    abstract::This study addresses two questions: 1) what is the maximum amount of tocopherol that can be contained in egg phosphatidylcholine liposomes, and 2) what is the stability of these vesicles in the presence of serum proteins? These liposomes, made with a French pressure cell, can contain no more than 33 mol% of tocopherol...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534254

    authors: Halks-Miller M,Guo LS,Hamilton RL Jr

    更新日期:1985-03-01 00:00:00

  • Analytical Method for Diacylglycerol Kinase ζ Activity in Cells Using Protein Myristoylation and Liquid Chromatography-Tandem Mass Spectrometry.

    abstract::Specific inhibitors of diacylglycerol kinase (DGK) ζ can be promising anticancer medications via the activation of cancer immunity. Although the detection of cellular activities of target enzymes is essential for drug screening in addition to in vitro assays, it is difficult to detect the activity of DGKζ in cells. In...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1002/lipd.12201

    authors: Honda S,Murakami C,Yamada H,Murakami Y,Ishizaki A,Sakane F

    更新日期:2019-11-01 00:00:00

  • Sterol composition of phaeodactylum tricornutum as influenced by growth temperature and light spectral quality.

    abstract::In a detailed sterol analysis of the marine diatom Phaeodactylum tricornutum, free sterols as well as esterified and glycosylated conjugates were found. When the alga was grown under standard conditions (i.e., at 13 degrees C under white light), 64% of total sterols were steryl glycosides. In all sterol classes, excep...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02522694

    authors: Véron B,Billard C,Dauguet JC,Hartmann MA

    更新日期:1996-09-01 00:00:00

  • Lactoperoxidase-catalyzed iodination of arachidonic acid: formation of macrolides.

    abstract::In the presence of iodide and hydrogen peroxide, lactoperoxidase catalyzed the conversion of arachidonic acid into several iodinated products; the major one was previously identified as an iodo-delta-lactone. Two minor and less polar products have now been characterized as 15-iodo-14-hydroxyeicosatrienoic acid, omega-...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535024

    authors: Boeynaems JM,Reagan D,Hubbard WC

    更新日期:1981-04-01 00:00:00

  • Regulation of cholesterol synthesis in isolated epithelial cells of human small intestine.

    abstract::We have investigated the regulation of cholesterol synthesis in isolated human small intestine epithelial cells (enterocytes). It was established that the amount of cholesterol synthesized increased linearly with the incubation time and the number of cells in the incubation mixture; the synthesis was suppressed by 7-k...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535408

    authors: Sviridov DD,Safonova IG,Talalaev AG,Repin VS,Smirnov VN

    更新日期:1986-12-01 00:00:00

  • Fatty acid status determination by cheek cell sampling combined with methanol-based ultrasound extraction of glycerophospholipids.

    abstract::Tissue and blood fatty acid compositions are used as biological markers of fatty acid intakes to improve dietary assessments. These approaches are invasive and not well accepted, particularly in infants and young children. We developed a sensitive method for the analysis of fatty acids in cheek cell glycerophospholipi...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-011-3576-7

    authors: Klingler M,Demmelmair H,Koletzko B,Glaser C

    更新日期:2011-10-01 00:00:00

  • Fatty acid synthetase of bovine mammary: Properties and products.

    abstract::Fatty acid synthetase purified (20 times) from lactating bovine mammary tissue had an approximate mot wt of 485,000. The enzyme had a high content of acidic and hydrophobic amino acid residues; 62±4 sulhydryl groups and one 4' phosphopantetheine residue/mole of enzyme. The enzyme was relatively stable when stored (3 m...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02532485

    authors: Kinsella JE,Bruns D,Infante JP

    更新日期:1975-04-01 00:00:00

  • Effects of dehydroepiandrosterone (DHEA) on hepatic lipid metabolism parameters and lipogenic gene mRNA expression in broiler chickens.

    abstract::The aim of the present study was to identify the effects of dehydroepiandrosterone (DHEA) on hepatic lipid metabolism parameters and lipogenic gene mRNA expression in broiler chickens. A total of 72 1-day-old broiler chicks received a common basal diet with DHEA added at either 0 (control), 5 or 20 mg/kg feed. In the ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-007-3104-y

    authors: Tang X,Ma H,Zou S,Chen W

    更新日期:2007-11-01 00:00:00

  • Enzymatic acylation of ether and ester lysophospholipids in rat liver microsomes.

    abstract::The acylation of lysophospholipids by rat liver acyltransferases was studied. A comparison between ester and ether lysophospholipids as substrates revealed large differences in substrate properties. For instance, oleic acid from oleoyl-CoA and arachidonic acid from arachidonoyl-CoA were not incorporated into 1-O-octad...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535535

    authors: Neumüller W,Fleer EA,Unger C,Eibl H

    更新日期:1987-11-01 00:00:00

  • Analytic ultracentrifuge calibration and determination of lipoprotein-specific refractive increments.

    abstract::Accurate quantification of the major classes and subfractions of human serum lipoproteins is an important analytical need in the characterization and evaluation of therapy of lipid and lipoprotein abnormalities. For calibrating the analytic ultracentrifuge (AnUC), we routinely use a Beckman calibration wedge cell with...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534491

    authors: Kahlon TS,Adamson GL,Glines LA,Lindgren FT,Laskaris MA,Shore VG

    更新日期:1984-07-01 00:00:00

  • Evidence for the mitochondrial biosynthesis of 3R-hydroxy-5Z,8Z,11Z,1 4Z-eicosatetraenoic acid in the yeast Dipodascopsis uninucleata.

    abstract::The biosynthesis of 3R-hydroxy-5Z, 8Z, 11Z,14Z-eicosatetraenoic acid (3R-HETE) from arachidonic acid (20:4n-6) by the hyphal-forming yeast, Dipodascopsis uninucleata, in cell-free enzyme extracts required CoASH, ATP, NAD+ and Mg2+; 3R-HETE was present as the CoA derivative in enzyme extracts and its biosynthesis was a...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-000-0637-4

    authors: Fox SR,Hamberg M,Friend J,Ratledge C

    更新日期:2000-11-01 00:00:00

  • Incorporation of fatty acids into phospholipids in L cells stimulated by antibody.

    abstract::Binding antibodies to surface membranes stimulated incorporation of fatty acids (FA) into phospholipids of L cells. Antibodies stimulated at least a 3.4-fold greater incorporation of arachidonic acid into phosphatidylinositol than into any other class of phospholipid when compared on a molar basis (p less than 0.003)....

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534451

    authors: Shearer WT,Ulrich RG

    更新日期:1984-04-01 00:00:00

  • Neonatal polyunsaturated fatty acid metabolism.

    abstract::The importance of n-6 and n-3 polyunsaturated fatty acids (PUFA) in neonatal development, particularly with respect to the developing brain and retina, is well known. This review combines recent information from basic science and clinical studies to highlight recent advances in knowledge on PUFA metabolism and areas w...

    journal_title:Lipids

    pub_type: 杂志文章,评审

    doi:10.1007/s11745-999-0348-x

    authors: Innis SM,Sprecher H,Hachey D,Edmond J,Anderson RE

    更新日期:1999-02-01 00:00:00

  • Effects of growth temperature on the fatty acid composition of the free-living nematode Caenorhabditis elegans.

    abstract::The effects of growth temperature on the fatty acid compositions of the phosphatidylcholine (PC), phosphatidylethanolamine (PE), and total lipid (TL) fractions of the free-living nematode Caenorhabditis elegans were investigated. A reduction in growth temperature from 25 to 15 degrees C caused the proportions of eicos...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02524292

    authors: Tanaka T,Ikita K,Ashida T,Motoyama Y,Yamaguchi Y,Satouchi K

    更新日期:1996-11-01 00:00:00

  • The questionable role of a microsomal delta8 acyl-coA-dependent desaturase in the biosynthesis of polyunsaturated fatty acids.

    abstract::Several experimental approaches were used to determine whether rat liver and testes express an acyl-CoA-dependent delta8 desaturase. When [1-14C]5,11,14-eicosatrienoic acid was injected via the tail vein, or directly into testes, it was incorporated into liver and testes phospholipids, but it was not metabolized to ot...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-000-0596-9

    authors: Chen Q,Yin FQ,Sprecher H

    更新日期:2000-08-01 00:00:00

  • Analysis of fatty acids in 12 Mediterranean fish species: advantages and limitations of a new GC-FID/GC-MS based technique.

    abstract::When fatty acids in fish are analyzed, results in percentage form (profile analysis) are mostly reported. However, the much more useful results expressed as mg/100 g (absolute analysis) is the main information required. Absolute methods based on calibration curves are of good accuracy but with a high degree of complex...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-012-3679-9

    authors: Nevigato T,Masci M,Orban E,Di Lena G,Casini I,Caproni R

    更新日期:2012-07-01 00:00:00