A specific, sensitive and high-capacity immunoassay for PAF.

Abstract:

:A specific radioimmunoassay for platelet-activating factor (PAF) sensitive in the range 10-1000 pg (0.02-2 pmoles) has been developed. Detailed quantitative hapten inhibition studies showed specificity for the acetyl group at C-2 of PAF, a requirement for the ether linkage at C-1 and some tolerance for substituents on the choline nitrogen. No significant cross-reactivity was found with phosphatidylcholine and lysophosphatidylcholine or with lysoPAF.

journal_name

Lipids

journal_title

Lipids

authors

Baldo BA,Smal MA,McCaskill AC

doi

10.1007/BF02536517

subject

Has Abstract

pub_date

1991-12-01 00:00:00

pages

1136-9

issue

12

eissn

0024-4201

issn

1558-9307

journal_volume

26

pub_type

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