Critical analysis of phospholipid hydrolyzing activities in ripening tomato fruits. Study by spectrofluorimetry and high-performance liquid chromatography.

Abstract:

:Using the spectrofluorimetric method described by Wittenauer et al. [Wittenauer, L.A., Shirai, K., Jackson, R.L., and Johnson, J.D. (1984) Biochem. Biophys. Res. Commun. 118, 894-901] for phospholipase A2 (PLA2) measurement, we have detected a phospholipase activity in Ailsa Craig and in mutant rin tomatoes at their normal harvest time (mature green stage). This activity in Ailsa Craig tomatoes increased at the beginning of fruit ripening (green-orange stage) and then decreased slowly. The decrease in activity, however, was greater when ripening occurred after tomato picking at normal harvest time than when ripening occurred on tomato plants. This phospholipase activity was always higher in rin tomatoes than in normal ones. Thin-layer chromatography of compounds obtained after incubation of tomato extract demonstrated a decrease in the substrate 1-acyl-2-(6[(7-nitro-2,1,3 benzoxadiazol-4-yl)amino]-caproyl)-sn-glycero-3-phosphocholine (C6-NBD-PC), and an increase in one product (NBD-aminohexanoic acid), but failed to detect the second product (1-acyl-sn-glycero-3-phosphocholine). We, therefore, developed a new one-step method for separation and quantification of a mixture of phospholipids and other lipids, using straight-phase-high-performance liquid chromatography with light-scattering detection. This method detected another fatty acid-releasing activity in enzyme extract from green-orange tomatoes. This lipolytic enzyme (or family of enzymes) slowly produced free fatty acids when 1-oleoyl-sn-glycero-3-phosphocholine was added as substrate. The production of fatty acids was stoichiometric and more rapid when 1-oleoyl-sn-glycero-3-phosphate and 1-oleoyl-sn-glycerol were used as substrates.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Lipids

journal_title

Lipids

authors

Rouet-Mayer MA,Valentova O,Simond-Côte E,Daussant J,Thévenot C

doi

10.1007/BF02537801

subject

Has Abstract

pub_date

1995-08-01 00:00:00

pages

739-46

issue

8

eissn

0024-4201

issn

1558-9307

journal_volume

30

pub_type

杂志文章

相关文献

LIPIDS文献大全
  • A review of tobacco BY-2 cells as an excellent system to study the synthesis and function of sterols and other isoprenoids.

    abstract::In plants, two pathways are utilized for the synthesis of isopentenyl diphosphate (IPP), the universal precursor for isoprenoid biosynthesis. In this paper we review findings and observations made primarily with tobacco BY-2 cells (TBY-2), which have proven to be an excellent system in which to study the two biosynthe...

    journal_title:Lipids

    pub_type: 杂志文章,评审

    doi:10.1007/s11745-004-1289-0

    authors: Hemmerlin A,Gerber E,Feldtrauer JF,Wentzinger L,Hartmann MA,Tritsch D,Hoeffler JF,Rohmer M,Bach TJ

    更新日期:2004-08-01 00:00:00

  • Changes in cultured arterial smooth muscle cells isolated from chicks upon cholesterol feeding.

    abstract::We have developed cultures of smooth muscle cells (SMC) isolated from arterial hypercholesterolemic chicks (cholesterol-SMC). These cultures are suitable for the study at the molecular level of the changes in arterial SMC induced by a cholesterol diet. By using a strong dose of cholesterol (5%) for 10 d, we obtained v...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-998-0194-x

    authors: Carazo A,Alejandre J,Diaz R,Ríos A,Castillo M,Linares A

    更新日期:1998-02-01 00:00:00

  • Impact of Fabp1 Gene Ablation on Uptake and Degradation of Endocannabinoids in Mouse Hepatocytes.

    abstract::Liver fatty-acid-binding protein (FABP1, L-FABP) is the major cytosolic binding/chaperone protein for both precursor arachidonic acid (ARA) and the endocannabinoid (EC) products N-arachidonoylethanolamine (AEA) and 2-arachidonoylglycerol (2-AG). Although FABP1 regulates hepatic uptake and metabolism of ARA, almost not...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1002/lipd.12071

    authors: McIntosh AL,Huang H,Landrock D,Martin GG,Li S,Kier AB,Schroeder F

    更新日期:2018-06-01 00:00:00

  • Differential alteration of delta 9 and delta 6 desaturation of fatty acids in rat brain preparations in vitro.

    abstract::Developing rat brain has the capacity for either delta 9 or delta 6 desaturation of fatty acids. In liver, evidence supports the existence of separate enzymes for each reaction, but it is not known whether in brain delta 9 or delta 6 desaturation of saturated fatty acids involves distinct enzymes. We have used fatty a...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533513

    authors: Cook HW

    更新日期:1979-09-01 00:00:00

  • Skin surface lipids of the dog.

    abstract::The skin surface lipid of the dog has been reported to contain a high proportion of diol diesters having a lower mobility on thin layer chromatography than diesters from other species in spite of containing similar fatty acid and diol components. In the present study, dog skin surface lipid was separated by preparativ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533265

    authors: Sharaf DM,Clark SJ,Downing DT

    更新日期:1977-10-01 00:00:00

  • Serum opacity factor enhances HDL-mediated cholesterol efflux, esterification and anti inflammatory effects.

    abstract::Serum opacity factor (SOF) is a streptococcal protein that disrupts the structure of human high density lipoproteins (HDL) releasing lipid-free apo A-I while forming a large cholesteryl ester-rich particle and a small neo HDL. Given its low cholesterol and high phospholipid contents, we tested the hypotheses that neo ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-010-3484-2

    authors: Tchoua U,Rosales C,Tang D,Gillard BK,Vaughan A,Lin HY,Courtney HS,Pownall HJ

    更新日期:2010-12-01 00:00:00

  • The relative incorporation of arachidonic and eicosapentaenoic acids into human platelet phospholipids.

    abstract::The incorporation of arachidonic acid (AA) as compared to eicosapentaenoic acid (EPA) into human platelet phospholipids was tested by incubating washed platelets with a known mixture of [3H]AA and [14C]EPA. Following incubation, the platelet lipids were extracted, the individual phospholipids--phosphatidylcholine (PC)...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534401

    authors: Weaver BJ,Holub BJ

    更新日期:1985-11-01 00:00:00

  • Differential Impact of Sex on Seasonal Changes in Mantle and Tentacle Phospholipids and Triacylglycerols in Sepia officinalis.

    abstract::The aim of the present work was to study the effect of season on phospholipids and triacylglycerols (TAG) of mantle and tentacles of female and male wild Sepia officinalis. The identified phospholipids were phosphatidylethanolamine (PtdEtn), phosphatidylcholine (PtdCho), phosphatidylserine (PtdSer), and phosphatidylin...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1002/lipd.12137

    authors: Tir M,El Cafsi I,Ammar RB,Mejri H,Tlili N,El Cafsi M

    更新日期:2019-02-01 00:00:00

  • Effects of administration of hypolipidemic agent, 2,2'''- [ (1-methyl-4, 4-diphenylbutylidene) bis (p-phenyleneoxy)] bistriethylamine oxalate (SQ 10,591) upon cholesterol esterification by aorta, adrenal, and testes of cholesterol-fed rabbits].

    abstract::Administration of 2,2'''-([1-methyl-4, 4-diphenylbutylidene] bis(p-phenyl-eneoxy]) bistriethylamine oxalate (SQ 10,591) at 20 mg/Kg daily for 5 days to cholesterol-fed rabbits resulted in no change in aortic microsomal cholesterol esterification with a palmitoyl coenzyme A substrate or of aortic mitochondrial choleste...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02532164

    authors: Morin RJ

    更新日期:1975-02-01 00:00:00

  • An increased incidence of apolipoprotein E2/E2 and E4/E4 in retinitis pigmentosa.

    abstract::Previous studies from our laboratory have shown that retinitis pigmentosa (RP), a family of hereditary retinal degenerations, is often accompanied by abnormal levels of cholesterol or polyunsaturated fatty acids. The requirement of the retina for n-3 fatty acids is well known, and a defect in the supply of these lipid...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02537120

    authors: Huq L,McLachlan T,Hammer HM,Bedford D,Packard CJ,Shepherd J,Converse CA

    更新日期:1993-11-01 00:00:00

  • Relationship between mouse liver delta 9 desaturase activity and plasma lipids.

    abstract::This study was undertaken to investigate the total plasma fatty acid composition and the relationship between plasma triacylglycerol (TG) levels and liver delta 9 desaturase activity in mice fed n-3 and/or n-6 fatty acid or hydrogenated coconut oil (HCO) (maximum 25 mg/g) supplemented diets. Generally, plasma TG level...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02536312

    authors: de Antueno RJ,Cantrill RC,Huang YS,Elliot M,Horrobin DF

    更新日期:1993-04-01 00:00:00

  • Changes in liver fatty acid unsaturation after partial hepatectomy in the rat.

    abstract::The purpose of this study was to assess changes in the degree of fatty acid unsaturation in rat liver after partial hepatectomy. This is the first study in which liver fatty acid unsaturation has been analyzed over a long period of regeneration until day 28 after operation. The relationship between changes in unsatura...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-000-543-9

    authors: Kishino T,Tanno M,Yamada H,Saito S,Matsumoto S

    更新日期:2000-04-01 00:00:00

  • Enrichment of LDL with EPA and DHA decreased oxidized LDL-induced apoptosis in U937 cells.

    abstract::Oxidized LDL (oxLDL) may contribute to the accumulation of apoptotic cells in atherosclerotic plaques. Although it is well established in monophasic chemical systems that the highly unsaturated EPA and DHA will oxidize more readily than FA that contain fewer double bonds, our previous studies showed that enrichment of...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-002-0962-7

    authors: Wu T,Geigerman C,Lee YS,Wander RC

    更新日期:2002-08-01 00:00:00

  • Decrease in alkaline triglyceride lipase in primary cultured hepatocytes from mice with sarcoma 180.

    abstract::Primary cultured hepatocytes from normal mice and mice with Sarcoma 180 were characterized. The viability of freshly isolated hepatocytes from both sources was over 90% and the cells had a relatively stable population of DNA for a minimum of three days. After incubation with (3H)leucine, the syntheses and secretions o...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534229

    authors: Masuno H,Okuda H

    更新日期:1985-07-01 00:00:00

  • Effect of dietary alpha-linolenate/linoleate balance on crescent type-anti-glomerular basement membrane nephritis in rats.

    abstract::Rats were fed diets with three different ratios of alpha-linolenate (18:3 n-3) and linoleate (18:2 n-6), and then crescentic-type anti-glomerular basement membrane (GBM) nephritis was induced. The urinary protein levels and the plasma urea nitrogen levels were significantly higher, and histological abnormalities of gl...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02544386

    authors: Watanabe S,Suzuki E,Kojima R,Suzuki Y,Okuyama H

    更新日期:1990-05-01 00:00:00

  • Protein engineering and applications of Candida rugosa lipase isoforms.

    abstract::Commercial preparations of Candida rugosa lipase (CRL) are mixtures of lipase isoforms used for the hydrolysis and synthesis of various esters. The presence of variable isoforms and the amount of lipolytic protein in the crude lipase preparations lead to a lack of reproducibility of biocatalytic reactions. Purificatio...

    journal_title:Lipids

    pub_type: 杂志文章,评审

    doi:10.1007/s11745-004-1258-7

    authors: Akoh CC,Lee GC,Shaw JF

    更新日期:2004-06-01 00:00:00

  • High-Fat Diet from Weaning until Early Adulthood Impairs T Cell Development in the Thymus.

    abstract::The growing prevalence of childhood obesity has become a serious health problem over the past few decades. Although the immune system is greatly affected by childhood obesity, whether obesity influences the T cell development in the thymus is poorly understood. In this study, we used a high-fat diet (HFD)-induced obes...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1002/lipd.12193

    authors: Li H,Wetchapinant C,Zhang L,Wu K

    更新日期:2020-01-01 00:00:00

  • Hydrocarbon chain distribution of ether phospholipids of the ascidian Halocynthia roretzi and the sea urchin Strongylocentrotus intermedius.

    abstract::The contents and compositions of the 1-O-alk-1'-enyl-2-acyl, 1-O-alkyl-2-acyl, and 1,2-diacyl glycerophospholipids in the muscle and viscera of the ascidian Halocynthia roretzi, and of the gonad of the sea urchin Strongylocentrotus intermedius, which are eaten to some extent in Alaska and in Asia, were analyzed by gas...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02522404

    authors: Jeong BY,Ohshima T,Koizumi C

    更新日期:1996-01-01 00:00:00

  • Eicosapentaenoic and docosahexaenoic acid affect mitochondrial and peroxisomal fatty acid oxidation in relation to substrate preference.

    abstract::Decreased triacylglycerol synthesis within hepatocytes due to decreased diacylglycerol acyltransferase (DGAT) activity has been suggested to be an important mechanism by which diets rich in fish oil lower plasma triacylglycerol levels. New findings suggest that eicosapentaenoic acid (EPA), and not docosahexaenoic acid...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-999-0445-x

    authors: Madsen L,Rustan AC,Vaagenes H,Berge K,Dyrøy E,Berge RK

    更新日期:1999-09-01 00:00:00

  • CETP and LCAT Gene Polymorphisms Are Associated with High-Density Lipoprotein Subclasses and Acute Coronary Syndrome.

    abstract::We evaluated whether CETP and LCAT gene polymorphisms are statistically associated with the high-density lipoprotein (HDL) size distribution, the cholesterol level of HDL subclasses, and the acute coronary syndrome (ACS) susceptibility. Two CETP gene polymorphisms (rs4783961 and rs708272) and one LCAT polymorphism (rs...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1002/lipd.12017

    authors: Vargas-Alarcon G,Perez-Mendez O,Herrera-Maya G,Garcia-Sanchez C,Martinez-Rios MA,Peña-Duque MA,Posadas-Sanchez R,Posadas-Romero C,Escobedo G,Fragoso JM

    更新日期:2018-02-01 00:00:00

  • Formation of cleavage products by autoxidation of lycopene.

    abstract::The cleavage products formed by autoxidation of lycopene were evaluated in order to elucidate possible oxidation products of lycopene in biological tissues. Lycopene solubilized at 50 microM in toluene, aqueous Tween 40, or liposomal suspension was oxidized by incubating at 37 degrees C for 72 h. Among a number of oxi...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-001-0706-8

    authors: Kim SJ,Nara E,Kobayashi H,Terao J,Nagao A

    更新日期:2001-02-01 00:00:00

  • Potential for employing the distribution of anomalous non-methylene-interrupted dienoic fatty acids in several marine invertebrates as part of food web studies.

    abstract::A group of homologous, nonmethylene-interrupted dienoic fatty acids (NMID) recently reported in oysters has been found in several other shellfish species and also in other marine phyla. The distribution of most other "normal" fatty acids among several species of shellfish is basically similar but mantle lipids from tw...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533289

    authors: Paradis M,Ackman RG

    更新日期:1977-02-01 00:00:00

  • Adipose monoacylglycerol:acyl-coenzyme A acyltransferase activity in the white-throated sparrow (Zonotrichia albicollis): characterization and function in a migratory bird.

    abstract::Although migrating birds use stored triacylglycerol as their primary fuel for flight, they must retain sufficient stores of omega 6 and omega 3 fatty acids to sustain reproduction after the spring migration. Hepatic monoacylglycerol:acyl-coenzyme A acyltransferase (EC 2.3.1.22) (MGAT) activity is associated with physi...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02536701

    authors: Mostafa N,Bhat BG,Coleman RA

    更新日期:1994-11-01 00:00:00

  • Working with Randy: The Diacylglycerol Acyltransferase Story.

    abstract::Vegetable oils are one of the main agricultural commodities. Demand has been increasing steadily over the last five decades and, with finite land available, it is vital that we increase productivity. My laboratory has focused on the regulation of plant lipid metabolism and, as part of this work, we identified diacylgl...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1002/lipd.12267

    authors: Harwood JL

    更新日期:2020-09-01 00:00:00

  • Docosapentaenoic acid does not completely replace DHA in n-3 FA-deficient rats during early development.

    abstract::The reciprocal replacement of DHA by docosapentaenoic acid (DPAn-6) was studied in rats that consumed an n-3 FA-deficient or n-3 FA-adequate diet. Dams were fed the two experimental diets from weaning and throughout pregnancy and lactation. Their pups were then fed the respective diets after weaning. Cortex FA analysi...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-003-1080-2

    authors: Greiner RS,Catalan JN,Moriguchi T,Salem N Jr

    更新日期:2003-04-01 00:00:00

  • Sources of triacylglycerol accumulation in livers of rats fed a cholesterol-supplemented diet.

    abstract::The source of free fatty acids (FFA) and the pathways contributing to the accumulation of neutral fats in livers of rats fed a cholesterol-enriched diet were investigated in this report. Supplementation with 1% cholesterol in the diet for four weeks resulted in hepatomegaly in the rats. The contents of cholesterol and...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02537027

    authors: Liu CH,Huang MT,Huang PC

    更新日期:1995-06-01 00:00:00

  • Lactoperoxidase-catalyzed iodination of arachidonic acid: formation of macrolides.

    abstract::In the presence of iodide and hydrogen peroxide, lactoperoxidase catalyzed the conversion of arachidonic acid into several iodinated products; the major one was previously identified as an iodo-delta-lactone. Two minor and less polar products have now been characterized as 15-iodo-14-hydroxyeicosatrienoic acid, omega-...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535024

    authors: Boeynaems JM,Reagan D,Hubbard WC

    更新日期:1981-04-01 00:00:00

  • Simvastatin therapy reduces prooxidant-antioxidant balance: results of a placebo-controlled cross-over trial.

    abstract::Oxidative stress is thought to play an important role in atherogenesis. The statin group of cholesterol-lowering drugs have been shown to reduce cardiovascular events and possess antioxidant properties. We aimed to assess the effects of simvastatin on a novel measure of prooxidant-antioxidant balance (PAB) in dyslipid...

    journal_title:Lipids

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s11745-010-3517-x

    authors: Parizadeh SM,Azarpazhooh MR,Moohebati M,Nematy M,Ghayour-Mobarhan M,Tavallaie S,Rahsepar AA,Amini M,Sahebkar A,Mohammadi M,Ferns GA

    更新日期:2011-04-01 00:00:00

  • Resolution of molecular species of intact serine and ethanolamine phosphatides by argentation chromatography of their trifluoroacetamides.

    abstract::An effective resolution of intact phosphatidylserines on the basis of unsaturation has been achieved by conventional argentation thin layer chromatography (TLC) following trifluoracetylaction. The trifluoroacetamides are prepared by treatment with trifluoroacetic anhydride or N-methyl-bis-trifluoroacetamide. The aceta...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533377

    authors: Yeung SK,Kuksis A,Marai L,Myher JJ

    更新日期:1977-07-01 00:00:00

  • 5c,11c,14c-eicosatrienoic acid and 5c,11c,14c,17c-eicosatetraenoic acid of Biota orientalis seed oil affect lipid metabolism in the rat.

    abstract::The effects of 5c,11c,14c-eicosatrienoic acid (20:3BSO) and 5c,11c,14c,17c-eicosatetraenoic acid (20:4BSO), polyunsaturated fatty acids (PUFA) contained in Biota orientalis seed oil (BSO), on lipid metabolism in rats were compared to the effects of fats rich in linoleic acid (LA) or alpha-linolenic acid (ALA) under si...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02536130

    authors: Ikeda I,Oka T,Koba K,Sugano M,Lie Ken Jie MS

    更新日期:1992-07-01 00:00:00