Abstract:
:Previous studies from our laboratory have shown that retinitis pigmentosa (RP), a family of hereditary retinal degenerations, is often accompanied by abnormal levels of cholesterol or polyunsaturated fatty acids. The requirement of the retina for n-3 fatty acids is well known, and a defect in the supply of these lipids (e.g., by apolipoproteins) could affect the course of the disease. The present study confirms and extends a report on apolipoprotein E (apo E) isoforms in German RP patients [Jahn, Oette, Esser, Bergmann, and Leiss, (1987) Ophthalmic Res. 19, 285-288] which showed a tenfold increased frequency of the E2/E2 phenotype compared to the average German population. In our study, apo E phenotypes were determined in the probands of 100 Scottish RP families. The findings revealed a 4-fold increase in the incidence of E2/E2 and an 8-fold increase in E4/E4 compared to a Scottish control population. These increases were statistically significant at the P < 0.05 and P < 0.01 levels, respectively. To investigate the possibility that some of these apparent E2/E2 or E4/E4 phenotypes might actually be new apo E mutations, we examined the behavior of the apo E on sodium dodecyl sulfate-polyacrylamide gels (E2 migrates anomalously) and on isoelectric focusing gels following cysteamine modification of cysteines. These studies showed that two RP patients possibly had new apo E mutations, though amino-terminal sequence analysis revealed no changes in the sequence of the first 19 residues; further sequence analysis is obviously warranted.
journal_name
Lipidsjournal_title
Lipidsauthors
Huq L,McLachlan T,Hammer HM,Bedford D,Packard CJ,Shepherd J,Converse CAdoi
10.1007/BF02537120subject
Has Abstractpub_date
1993-11-01 00:00:00pages
995-8issue
11eissn
0024-4201issn
1558-9307journal_volume
28pub_type
杂志文章相关文献
LIPIDS文献大全abstract::Differences in the positional incorporation of 9-trans[1-(14)C] octadecenoic (elaidic) and 9-trans,12-trans[1-(14)C] octadecadienoic (linoelaidic) acids in fetal lecithin of rats were demonstrated. On the 20th day of gestation, a 14C-labeled albumin complex of elaidic or linoelaidic acid was injected into the jugular ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535045
更新日期:1981-07-01 00:00:00
abstract::The pyrrolidide and picolinyl ester derivatives of the fatty acids in two natural lipid samples rich in unsaturated fatty acids, pig testis lipids and cod liver oil were satisfactorily resolved on capillary columns of fused silica coated with stationary phases of varying polarity. The picolinyl esters, in particular, ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537217
更新日期:1986-10-01 00:00:00
abstract::Sphingomyelin was isolated from cysts of the brine shrimp Artemia franciscana using QAE-Sephadex A25, Florisil and Iatrobeads column chromatographies. The chemical structure was identified using thin-layer chromatography, gas-liquid chromatography, infrared spectroscopy and matrix-assisted laser desorption ionization ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-010-3438-8
更新日期:2010-07-01 00:00:00
abstract::We have previously shown that adipose cells from young genetically obese Zucker rats are characterized by very high metabolic activity together with an increase in a wide range of membrane-mediated functions. The aim of the present study was to examine whether the physical properties of the membranes and the compositi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536730
更新日期:1994-03-01 00:00:00
abstract::An improved headspace capillary gas chromatographic (GC) method was developed to measure the oxidative susceptibility of human red blood cell (RBC) membranes. This method analyzed volatile peroxidation products of both n-6 (hexanal and pentane) and n-3 (propanal) polyunsaturated fatty acids. Oxidative susceptibility t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536076
更新日期:1991-06-01 00:00:00
abstract::Liver fatty-acid-binding protein (FABP1, L-FABP) is the major cytosolic binding/chaperone protein for both precursor arachidonic acid (ARA) and the endocannabinoid (EC) products N-arachidonoylethanolamine (AEA) and 2-arachidonoylglycerol (2-AG). Although FABP1 regulates hepatic uptake and metabolism of ARA, almost not...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12071
更新日期:2018-06-01 00:00:00
abstract::In the presence of iodide and hydrogen peroxide, lactoperoxidase catalyzed the conversion of arachidonic acid into several iodinated products; the major one was previously identified as an iodo-delta-lactone. Two minor and less polar products have now been characterized as 15-iodo-14-hydroxyeicosatrienoic acid, omega-...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535024
更新日期:1981-04-01 00:00:00
abstract::Normal phase high performance liquid chromatography methods are described for the separation of neutral lipid, fatty acid and five phospholipid classes using spectrophotometric detection at 206 nm. Separations were accomplished in less than 10 min for each lipid class. A mobile phase consisting of hexane/methyltertiar...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535282
更新日期:1988-12-01 00:00:00
abstract::The acylation of lysophospholipids by rat liver acyltransferases was studied. A comparison between ester and ether lysophospholipids as substrates revealed large differences in substrate properties. For instance, oleic acid from oleoyl-CoA and arachidonic acid from arachidonoyl-CoA were not incorporated into 1-O-octad...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535535
更新日期:1987-11-01 00:00:00
abstract::1-O-Hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor, PAF) is known to stimulate platelet aggregation and serotonin release in concentrations ranging from 10(-10)-10(-5) M. Since a variety of synthetic PAF analogues are potent antineoplastic agents in vitro and in vivo, it was the aim of th...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535546
更新日期:1987-11-01 00:00:00
abstract::Subcellular membranes were analyzed for their lipid composition and protein content at two developmental points representing the third instar wandering larvae and prepupal stages of Drosophila. At both stages, phosphatidylethanolamine (PE) and phosphatidylcholine (PC) were the major constituents with phosphatidylinosi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535576
更新日期:1992-12-01 00:00:00
abstract::The burrowing sponges Anthosigmella varians and Spheciospongia vesparium were found to be rich in the isoprenoid phospholipid fatty acid 4,8,12-trimethyltridecanoic (5.2% and 23%, respectively, of the total fatty acid composition), while the burrowing sponge Chondrilla nucula and the demosponge Agelas dispar contained...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533980
更新日期:1987-10-01 00:00:00
abstract::The objective of this study was to examine whether whole body oxidation rates of dietary linoleic, alpha-linolenic and oleic acids differ when the acids are provided in identical quantities. Male rats were fed for 10 wk a 15% fat (w/w) diet containing equal amounts of linoleic, alpha-linolenic and oleic acids (22.7, 2...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02578246
更新日期:1994-07-01 00:00:00
abstract::Mevalonic acid-derived intermediates in the cholesterol biosynthetic pathway have been recognized as being critical to the isoprenylation of a variety of growth-regulating proteins, including those of the RAS superfamily. Treatment of cells with lovastatin, a hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase inhibi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0879-1
更新日期:2002-02-01 00:00:00
abstract::The synthesis of thioether phospholipids, which represent a new class of antitumor agents, is reported here. In particular, the route of synthesis of 3-hexadecylmercapto-2-methoxymethylpropyl-2'-trimethylammoni o-ethyl phosphate (BM 41.440, Ilmofosine), one of the most potent cytostatic/cytotoxic derivatives, is descr...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535561
更新日期:1987-11-01 00:00:00
abstract::The lipid quality of ruminant products is largely determined by the extent of rumen microbial biohydrogenation (BH) of polyunsaturated fatty acids (FAs) and the substances formed thereby. In vitro batch incubations with mixed rumen bacteria were tracked over 24 h to characterize the profiles and kinetics of the BH pro...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-007-3032-x
更新日期:2007-04-01 00:00:00
abstract::The seed oil ofEuonymus verrucosus Scop., family Celastraceae, contains more than 90% 1,2-diacyl-3-acetins (monoacetotriglycerides). The constituent triglyceride acids, other than acetic, are the usual long-chain fatty acids.Thin-layer chromatography (TLC), infrared (IR) and hydrolysis with pancreatic lipase indicate ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533174
更新日期:1967-11-01 00:00:00
abstract::We have reported previously that platelet-activating factor (PAF) interacts with the neuronal cell line NG108-15 (neuroblastoma X glioma hybrid) and the pheochromocytoma cell line, PC12. PAF acts on these cells by raising levels of intracellular free calcium ions. In the present report, we extend these studies. PAF in...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536540
更新日期:1991-12-01 00:00:00
abstract::The current study evaluated the composition and relationships of polyunsaturated fatty acid biohydrogenation products (PUFA-BHP) from the perirenal (PRF) and subcutaneous fat (SCF) of yearling steers fed a 70 % grass hay diet with concentrates containing either sunflower-seed (SS) or flaxseed (FS). Analysis of varianc...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-013-3876-1
更新日期:2014-03-01 00:00:00
abstract::Saccharomyces cerevisiae cells were demonstrated to contain lysophosphatidylcholine (lysoPtdCho) acyltransferase (E.C. 2.3.1.23) activity. The enzyme displayed Km(app) of 69 microM for lysoPtdCho and 152 microM for oleoyl CoA. Enzyme activity was not affected by the addition of 1 mM Mg2+, Mn2+, Ca2+, or 200 mM EDTA. H...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0328-1
更新日期:1998-12-01 00:00:00
abstract::Lipids from bovine optic nerve were analyzed. The total content of 16.5% by weight included 27.2% nonpolar lipids, 26.1% glycolipids, and 46.7% phospholipids by weight. Free cholesterol was the major component of the nonpolar lipid fraction. The cerebrosides, 73.5% of total glycolipids, were separated by thin layer ch...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533745
更新日期:1978-10-01 00:00:00
abstract::Obesity and type 2 diabetes have reached epidemic proportions in the United States. It is well-established that increasing physical activity plays an important role in reducing risk of obesity and diabetes. Few studies, however, have examined the association between sedentary behaviors such as prolonged television (TV...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-003-1038-4
更新日期:2003-02-01 00:00:00
abstract::During our ongoing project on the biosynthesis of R-(+)-octane-1,3-diol the metabolism of linoleic acid was investigated in stored apples after injection of [1-14C]-, [9,10,12,13-3H]-,13C18- and unlabeled substrates. After different incubation periods the products were analyzed by gas chromatography-mass spectroscopy ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-999-0375-7
更新日期:1999-04-01 00:00:00
abstract::The amount and composition of lysophosphatidate present in different rat tissues have been estimated by an internal standard method in which a synthetic unnatural isomer (1-heptadecanoyl-rac-glycerol-3-phosphate) was added to the total lipid extracts, and the fatty acid composition of purified lysophosphatidate was de...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535172
更新日期:1989-04-01 00:00:00
abstract::Using an experimental model that enables the effects of alcohol to be distinguished from the effects of the nonalcoholic components present in wine, we determined whether wine has effects other than those of alcohol on the metabolism of cholesterol. Male rats were fed a standard diet and had free access to water and e...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0732-6
更新日期:2001-04-01 00:00:00
abstract::Active lipases were isolated from the culture supernatant of the basidiomycetous fungus Pleurotus sapidus by foam fractionation. The pH value, the gas flow, and the foaming period were systematically varied to optimize the transport of the enzymes into the foam phase. On a 70-mL scale, a maximum recovery of hydrolytic...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-005-1389-x
更新日期:2005-03-01 00:00:00
abstract::Flaxseed, echium, and canola oils contain alpha-linolenic acid (18:3n-3, ALA) in a range of concentrations. To examine their effect on elevating cardiac levels of long-chain n-3 FA, diets based on these n-3-containing vegetable oils were fed to rats for 4 wk. Sunflower oil, which contains little ALA, was a comparator....
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-005-1461-6
更新日期:2005-10-01 00:00:00
abstract::Studies are reported on the capacity of isolated rat renal papilla (inner medulla) to synthesize and release prostaglandin (PG) E from endogenous and exogenous precursor(s) during development of an essential fatty acid (EFA) deficiency in the rat. Weanling (21-day-old) male Sprague-Dawley rats were fed a fat-free diet...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533845
更新日期:1978-12-01 00:00:00
abstract::The purpose of the present study was to investigate the effects of gamma linolenic acid (GLA) on cardiovascular responses to psychosocial stress (isolation) and to pressor hormones in the genetically borderline hypertensive rat (SHR X WKY). Adult male SHR X WKY were divided into two groups following five weeks of grou...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534282
更新日期:1985-09-01 00:00:00
abstract::Ceramides are a family of signalling lipids with diverse physiological functions that include pro-differentiative and pro-apoptotic signalling. Ceramides and their derivatives are major constituents of myelin, maintaining neuronal conductivity. Ceramides are synthesized by ceramide synthases, of which there are six is...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3969-5
更新日期:2015-01-01 00:00:00