Headspace gas chromatography of volatile lipid peroxidation products from human red blood cell membranes.

abstract：:31P Nuclear magnetic resonance (NMR) spectra of human erythrocyte lysates dissolved in sodium cholate were acquired. The narrow resonances of phospholipids were mostly well resolved, allowing identification and accurate quantitative analysis of phospholipid classes of the erythrocyte membranes. The ether-linked phosph...

journal_title：Lipids

authors： Nouri-Sorkhabi MH,Wright LC,Sullivan DR,Kuchel PW

更新日期：1996-07-01 00:00:00

abstract：:Recently, significant differences between rats fed a casein diet and rats fed a soybean protein diet have been observed in hepatic phospholipid fatty acid patterns (Sjöblom, L., and Eklund, A., Biochim. Biophys. Acta 1004, 187-192, 1990). The influence of these two diets on the delta 6 desaturase activity was investig...

journal_title：Lipids

authors： Lindholm M,Eklund A

更新日期：1991-02-01 00:00:00

abstract：:For three weeks, male Sprague-Dawley rats at either four weeks (young) or eight months (adult) of age were pair-fed one of the purified diets free of or containing either 0.2% of oxidized cholesterol mixture (cholesterol oxidation products) or 0.2% of cholesterol. Although the food intake was similar, dietary oxidized...

journal_title：Lipids

authors： Osada K,Kodama T,Noda S,Yamada K,Sugano M

更新日期：1995-05-01 00:00:00

abstract：:This paper represents the first synthesis, spectroscopic characterization, and antitumor evaluation of F-, N-, and S-containing C4alpha-FA derivatives of podophyllotoxin. In a synthetic strategy, a FA unit of 4-O-podophyllotoxinyl 12-hydroxyoctadec-Z-9-enoate 2, a derivative of podophyllotoxin, was functionalized at t...

journal_title：Lipids

authors： Mustafa J,Khan SI,Ma G,Walker LA,Khan IA

更新日期：2005-04-01 00:00:00

abstract：:Because of the known advantages of coupling high performance liquid chromatography with mass spectrometry (HPLC-MS) in biological fluids, studies on the reversed-phase HPLC-MS system for direct analysis of conjugated bile acids in human bile samples are described. Ten samples of gallbladder bile of apparently healthy ...

journal_title：Lipids

authors： Mingrone G,Greco AV,Boniforti L,Passi S

更新日期：1983-01-01 00:00:00

abstract：:Concentrations of synthetic antioxidants butylated hydroxyanisole, butylated hydroxytoluene, and tert-butyl hydroquinone were quantified using a high-performance liquid chromatograph with spectrofluorometric detector. The antioxidants were separated and eluted on a reversed-phase column by gradient of a mixture of H2O...

journal_title：Lipids

authors： Yankah VV,Ushio H,Ohshima T,Koizumi C

更新日期：1998-11-01 00:00:00

abstract：:Microtiter plate methods were developed for the enzymatic determination of serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and triglyceride (TG), and for the turbidometric determination of apolipoproteins. The micromethods resulted in accurate, precise values that were in good agreement with...

journal_title：Lipids

authors： Shireman RB,Durieux J

更新日期：1993-02-01 00:00:00

abstract：:Choline is an essential nutrient required for the biosynthesis of membrane lipid phosphatidylcholine (PtdCho). Here we elucidate the mechanism of how palmitic acid (PAM) and oleic acid (OLA) regulate choline transporter-like protein 1 (CTL1/SLC44A1) function. We evaluated the mechanism of extracellular and intracellul...

journal_title：Lipids

authors： Schenkel LC,Bakovic M

更新日期：2014-08-01 00:00:00

abstract：:Lipoproteins from the plasma of three nonlactating Holstein cows were isolated using either preparative ultracentrifugation or gel filtration chromatography. Lipoprotein classes obtained by ultracentrifugation were very low density plus chylomicra, less than 1.006 g/ml; low density, 1.007-1.039 g/ml; high density1, 1....

journal_title：Lipids

authors： Grummer RR,Davis CL,Hegarty HM

更新日期：1983-11-01 00:00:00

abstract：:Current methods for determining the regiochemistry of monoacylglycerols are lengthy, tedious and aggravated by the ready isomerization of 2-acyl-sn-glycerols. A new method employs a very rapid adsorption chromatography step in which isomerization is kept to a minimum, followed by formation of the isopropylidene deriva...

journal_title：Lipids

authors： Dawe RD,Wright JJ

更新日期：1988-04-01 00:00:00

abstract：:A novel phospholipid containing a chromanol structure at its polar head group was synthesized from egg yolk phosphatidylcholine and 2,5,7,8-tetramethyl-6-hydroxy-2-(hydroxyethyl)chroman by transphosphatidylation catalyzed by phospholipase D from Streptomyces lydicus. The structure of the product synthesized was shown ...

journal_title：Lipids

authors： Koga T,Nagao A,Terao J,Sawada K,Mukai K

更新日期：1994-02-01 00:00:00

abstract：:The reaction of alpha-tocopherol (alpha-T) with superoxide anion (O2-) in both dry acetonitrile and in aqueous acetonitrile solution is described. The O2- was generated by the electrochemical reduction of molecular oxygen in acetonitrile, using tetrabutylammonium bromide as an electrolyte. alpha-T was reacted with O2-...

journal_title：Lipids

authors： Csallany AS,Ha YL

更新日期：1992-03-01 00:00:00

abstract：:Twenty-three of 86 strains of lactic acid bacteria transformed linoleic acid into hydroxy FA. Two distinct conversion pathways were in operation. Two strains of Lactobacillus acidophilus and a strain of Pediococcus pentosaceus produced 13(S)-hydroxy-9-octadecenoic acid 113(S)-OH 18:11 and 10,13dihydroxyoctadecanoic ac...

journal_title：Lipids

authors： Kishimoto N,Yamamoto I,Toraishi K,Yoshioka S,Saito K,Masuda H,Fujita T

更新日期：2003-12-01 00:00:00

abstract：:The enzyme(s) present in acetonedried powder of rat and rabbit aortas, which catalyzes the synthesis and hydrolysis of cholesteryl ester, was purified partially by acid precipitation, acetone fractionation, O-(diethylaminoethyl) cellulose chromatography, and Sephadex G-100 filtration. The synthetic activity was purifi...

journal_title：Lipids

authors： Kothari HV

更新日期：1975-06-01 00:00:00

abstract：:In offspring exposed in utero to a maternal diet high in fat (HF), we have previously demonstrated that despite similar birth weights, HF adult offspring at 6 months of age had significantly higher body weights, greater adiposity, and increased triacylglycerol (TAG) levels as compared to controls. We hypothesized that...

journal_title：Lipids

authors： Seet EL,Yee JK,Jellyman JK,Han G,Ross MG,Desai M

更新日期：2015-06-01 00:00:00

abstract：:The aim of this study was to determine the effects of an omega-3 (n-3) polyunsaturated fatty acid (PUFA)-enriched diet on animal fat depots and lipid oxidation in the blood and meat of broiler chickens. Abdominal fat pad (AFP), sartorius muscle and liver histology were used to assess the effect of the dietary fat on a...

journal_title：Lipids

authors： González-Ortiz G,Sala R,Cánovas E,Abed N,Barroeta AC

更新日期：2013-07-01 00:00:00

abstract：:Hydrolysis of cell envelope phospholipids was demonstrated in cells of both autolytic and nonautolytic strains of Neisseria gonorrhoeae that were labeled during growth in the presence of [3H] acetate. The label incorporated into the cellular phospholipids was located exclusively in the fatty acid acyl side chains. Lab...

journal_title：Lipids

authors： Cacciapuoti AF,Wegener WS,Morse SA

更新日期：1979-08-01 00:00:00

abstract：:In humans and animals, intestinal flora is indispensable for bile acid transformation. The goal of our study was to establish gnotobiotic mice with intestinal bacteria of human origin in order to examine the role of intestinal bacteria in the transformation of bile acids in vivo using the technique of gnotobiology. Ei...

journal_title：Lipids

authors： Narushima S,Itoha K,Miyamoto Y,Park SH,Nagata K,Kuruma K,Uchida K

更新日期：2006-09-01 00:00:00

abstract：:A simple and sensitive method to analyze mixtures of desmosterol, 7-dehydrocholesterol and cholesterol is described. The method involves the oxidative conversion of the sterols with cholesterol oxidase, followed by high performance liquid chromatographic (HPLC) analysis. A C18 reversed phase column (3 microns, 75 X 4....

journal_title：Lipids

authors： Goh EH,Colles SM,Otte KD

更新日期：1989-07-01 00:00:00

abstract：:The resolution of a series of racemic 1-alkyl-3-trityl-rac-glycerols and 1-alkyl-2-benzyl-rac-glycerols into their enantiomers by high-performance liquid chromatography (HPLC) was investigated. Of the racemic glycerol derivatives studied, only three (1,2-0-isopropylidene-rac-glycerol, 1,2-0-isopropylidene-3-benzyl-rac...

journal_title：Lipids

authors： Foglia TA,Maeda K

更新日期：1991-09-01 00:00:00

abstract：:Total lipids from whole pig adrenal glands as well as from their mitochondria, microsomes, liposomes, and cell sap were extracted and fractionated first into neutral lipids and phospholipids. The highest percentage of neutral lipids was found in the cell sap, and the lowest in the microsomal fraction. Neutral lipids w...

journal_title：Lipids

authors： Cmelik SH,Ley H

更新日期：1975-11-01 00:00:00

abstract：:The present study examined the effect of varying dietary linoleate intake (0.01, 0.24, 2.4, 24, 80 or 160 g/kg diet) for 24 weeks on the distribution of triacylglycerol (TG) molecular species in rat epididymal adipose tissue. Adipose TG fractions were purified by thin-layer chromatography and separated into different ...

journal_title：Lipids

authors： Huang YS,Lin X,Smith RS,Redden PR,Jenkins DK,Horrobin DF

更新日期：1992-09-01 00:00:00

abstract：:There is convincing evidence that normal cultured human keratinocytes possess a 15-lipoxygenase activity which, however, does not appear to manifest itself without cell membrane damage. When "activated", this enzyme transforms arachidonic acid into 15-hydroxyeicosatetraenoic acid (15-HETE), and linoleic acid into 13-h...

journal_title：Lipids

authors： Green FA

更新日期：1990-10-01 00:00:00

abstract：:Peroxisome proliferator-activated receptor alpha (PPARalpha) belongs to the nuclear receptor superfamily that regulates multiple target genes involved in lipid metabolism. Cholesterol ester transfer protein (CETP) is a secreted glycoprotein that modifies high-density lipoprotein (HDL) particles. In humans, plasma CETP...

journal_title：Lipids

authors： Beyer TP,Chen Y,Porter RK,Lu D,Schmidt RJ,Mantlo NB,Konrad RJ,Cao G

更新日期：2008-07-01 00:00:00

abstract：:This study examined the effects of five CLA isomers and the non-conjugated LA on nitric oxide (NO) production, an important modulator of vasodilation, inflammation, and cytotoxicity. Bovine aortic endothelial cells (BAECs) were pretreated with pure CLAs (c9, c11-, c9, t11-, t9, t11-, t10, c12-, c11, c13-CLA) and the n...

journal_title：Lipids

authors： Jenko KJ,Vanderhoek JY

更新日期：2008-04-01 00:00:00

abstract：:The effect of dietary n-3 FA deficiency on bone tissue FA composition was evaluated in growing rats. Two mixtures combining hydrogenated coconut oil with safflower oil served as the n-3-deficient dietary treatments and provided two levels of linoleic acid (LA). The n-3 treatments were formulated with added alpha-linol...

journal_title：Lipids

authors： Li Y,Greiner RS,Salem N Jr,Watkins BA

更新日期：2003-06-01 00:00:00

abstract：:A new method of isoelectric focusing (IEF) and pH gradient electrophoresis, using thin layers of agarose gel beads, was devised to investigate chylomicrons and very low density lipoproteins (VLDL). pH gradient stability and cathodal gradient drift were similar to those of polyacrylamide gel IEF, and linearity of gradi...

journal_title：Lipids

authors： Vost A,Pocock DM,Pleet S

更新日期：1979-10-01 00:00:00

abstract：:Ceramides are the major type of lipid found in stratum corneum from the skin, gingiva and hard palate. The present study examined the ceramides of the stratum corneum from the hard palate. Six fractions of ceramides were isolated by preparative thin-layer chromatography. The least polar fraction contained an unusual a...

journal_title：Lipids

authors： Hill JR,Wertz PW

更新日期：2009-03-01 00:00:00

abstract：:Cholesterol biosynthesis from acetate-2-(14)C by the livers of suckling rats, which is known to be relatively slow, was increased 2-3-fold within 24 hours after severing the bile duct. Cholesterol synthesis by sham-operated litter mates showed no change under similar treatment. Mevalonate biosynthesis from acetate-2-(...

journal_title：Lipids

authors： Harris RA,Rivera ER,Villemez CL Jr,Quackenbush FW

更新日期：1967-03-01 00:00:00

abstract：:We have examined the possibility that human polymorphonuclear cells exposed to IgA immune complexes can mediate the production of platelet-activating factor (PAF) and oxygen radicals. We found that human IgA and IgG immune aggregates stimulated, to a similar extent, PAF and O2- production by human polymorphonuclear ce...

journal_title：Lipids

authors： Hernando P,Egido J,de Nicolas R,Gonzalez E

更新日期：1991-12-01 00:00:00