Deoxycholic acid formation in gnotobiotic mice associated with human intestinal bacteria.


:In humans and animals, intestinal flora is indispensable for bile acid transformation. The goal of our study was to establish gnotobiotic mice with intestinal bacteria of human origin in order to examine the role of intestinal bacteria in the transformation of bile acids in vivo using the technique of gnotobiology. Eight strains of bile acid-deconjugating bacteria were isolated from ex-germ-free mice inoculated with a human fecal dilution of 10(-6), and five strains of 7alpha-dehydroxylating bacteria were isolated from the intestine of limited human flora mice inoculated only with clostridia. The results of biochemical tests and 16S rDNA sequence analysis showed that seven out of eight bile acid-deconjugating strains belong to a bacteroides cluster (Bacteroides vulgatus, B. distasonis, and B. uniformis), and one strain had high similarity with Bilophila wadsworthia. All five strains that converted cholic acid to deoxycholic acid had greatest similarity with Clostridium hylemonae. A combination of 10 isolated strains converted taurocholic acid into deoxycholic acid both in vitro and in the mouse intestine. These results indicate that the predominant bacteria, mainly Bacteroides, in human feces comprise one of the main bacterial groups for the deconjugation of bile acids, and clostridia may play an important role in 7aplha-dehydroxylation of free-form primary bile acids in the intestine although these strains are not predominant. The gnotobiotic mouse with bacteria of human origin could be a useful model in studies of bile acid metabolism by human intestinal bacteria in vivo.






Narushima S,Itoha K,Miyamoto Y,Park SH,Nagata K,Kuruma K,Uchida K




Has Abstract


2006-09-01 00:00:00














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    pub_type: 杂志文章,评审


    authors: Gaullier JM,Berven G,Blankson H,Gudmundsen O

    更新日期:2002-11-01 00:00:00

  • Protectin DX, a double lipoxygenase product of DHA, inhibits both ROS production in human neutrophils and cyclooxygenase activities.

    abstract::Neutrophils play a major role in inflammation by releasing large amounts of reactive oxygen species (ROS) produced by NADPH oxidase (NOX) and myeloperoxidase (MPO). This ROS overproduction is mediated by phosphorylation of the NOX subunits in an uncontrolled manner. Therefore, targeting neutrophil subunits would repre...


    pub_type: 杂志文章


    authors: Liu M,Boussetta T,Makni-Maalej K,Fay M,Driss F,El-Benna J,Lagarde M,Guichardant M

    更新日期:2014-01-01 00:00:00

  • Biochemical modifications and transcriptional alterations attendant to sterol feeding in Phytophthora parasitica.

    abstract::Phytophthora species are eukaryotic sterol auxotrophs that possess the ability to grow, albeit poorly, in the complete absence of sterols. Growth of Phytophthora is often improved substantially when an exogenous source of sterol is provided. Additionally, sterols may be required for sexual and asexual sporulation in P...


    pub_type: 杂志文章


    authors: Dotson WD,Tove SR,Parks LW

    更新日期:2000-03-01 00:00:00

  • Sex- and age-related differences in ceramide dihexosides of primary human brain tumors.

    abstract::Neutral glycolipids (NGL) are promising diagnostic markers of human gliomas, but differences in NGL with age and sex have not been examined. Previous work demonstrated that ceramide dihexosides (CDH) levels in mouse kidney are age- and sex-dependent, probably due to levels of sex hormones. We quantitated CDH in 181 hu...


    pub_type: 杂志文章


    authors: Yates AJ,Franklin TK,Scheithauer BW,Burger PC,Pearl DK

    更新日期:1999-01-01 00:00:00

  • A diurnal variation of hepatic acid cholesteryl ester hydrolase activity in the rat.

    abstract::The diurnal variation in lysosomal acid cholesteryl ester hydrolase (Acid CEH), (EC has been examined in fed, fasted and adrenalectomized rats. The Acid CEH activity of normal rat liver exhibits a diurnal rhythm with maxima at 06.00 hours and minima at 18.00 hours, but such a rhythm was not observed in splee...


    pub_type: 杂志文章


    authors: Tanaka M,Yonekura R,Iio T,Tabata T

    更新日期:1985-01-01 00:00:00

  • High dietary cholesterol masks type 2 diabetes-induced osteopenia and changes in bone microstructure in rats.

    abstract::Type 2 diabetes mellitus (T2DM) often occurs concurrently with high blood cholesterol or dyslipidemia. Although T2DM has been hypothesized to impair bone microstructure, several investigations showed that, when compared to age-matched healthy individuals, T2DM patients had normal or relatively high bone mineral densit...


    pub_type: 杂志文章


    authors: Lapmanee S,Charoenphandhu N,Aeimlapa R,Suntornsaratoon P,Wongdee K,Tiyasatkulkovit W,Kengkoom K,Chaimongkolnukul K,Seriwatanachai D,Krishnamra N

    更新日期:2014-10-01 00:00:00

  • The essential oil from Tagetes minuta L. modulates the binding of [3H]flunitrazepam to crude membranes from chick brain.

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    pub_type: 杂志文章


    authors: García DA,Perillo MA,Zygadlo JA,Martijena ID

    更新日期:1995-12-01 00:00:00

  • Metabolism of 2,4-3H-14alpha-methyl-5alpha-ergost-8-enol and 2,4-3H-5alpha-ergosta-8,14-dienol in Ochromonas malhamensis.

    abstract::Tritium-labeled 14alpha-methyl-5alpha-ergost-8-enol and 5alpha-ergosta-8,14-dienol were converted to brassicasterol and poriferasterol in Ochromonas malhamensis. This indicates that the organism can rearrange these sterol structures so that they contain the naturally occurring 5(6) double bond, and suggests that O. ma...


    pub_type: 杂志文章


    authors: Adler JH,Patterson GW

    更新日期:1976-08-01 00:00:00

  • Comparison of ultracentrifugation and gel filtration for the isolation of bovine lipoproteins.

    abstract::Lipoproteins from the plasma of three nonlactating Holstein cows were isolated using either preparative ultracentrifugation or gel filtration chromatography. Lipoprotein classes obtained by ultracentrifugation were very low density plus chylomicra, less than 1.006 g/ml; low density, 1.007-1.039 g/ml; high density1, 1....


    pub_type: 杂志文章


    authors: Grummer RR,Davis CL,Hegarty HM

    更新日期:1983-11-01 00:00:00

  • Structural characterization of a "signature" phosphatidylethanolamine as the major 10-hydroxy stearic acid-containing lipid of Cryptosporidium parvum oocysts.

    abstract::A 10-hydroxy stearic acid-containing lipid from Cryptosporidium parvum was purified by thin-layer chromatography and analyzed by infrared spectroscopy, fast-atom bombardment mass spectrometry, 1H and 31P nuclear magnetic resonance spectroscopy, and was identified as phosphatidyl-ethanolamine. ...


    pub_type: 杂志文章


    authors: Schrum DP,Alugupalli S,Kelly ST,White DC,Fayer R

    更新日期:1997-07-01 00:00:00

  • A sardine oil-rich diet increases iron absorption but does not compensate the hypoferremia associated with inflammation.

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    pub_type: 杂志文章


    authors: Rodríguez MC,Sáiz MP,Mitjavila MT

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  • Essentiality of fatty acids.

    abstract::All fatty acids have important functions, but the term "essential" is applied only to those polyunsaturated fatty acids (PUFA) that are necessary for good health and cannot be completely synthesized in the body. The need for arachidonic acid, which is utilized for eicosanoid synthesis and is a constituent of membrane ...


    pub_type: 杂志文章,评审


    authors: Spector AA

    更新日期:1999-01-01 00:00:00

  • Inhibition of lymphocyte capping by fatty acids in mouse and man.

    abstract::Lipid-enriched diets have been related to a high cancer incidence in experimental animals for many years, and more recently, to assorted defects on the immune response. We investigated the effect of incubating human or murine (C3H/HEJ) lymphocytes with saturated (16:0) and unsaturated (18:1, 18:2, 18:3, 20:4) fatty ac...


    pub_type: 杂志文章


    authors: Santiago-Delpín EA,Román-Franco AA,Colón JI

    更新日期:1982-10-01 00:00:00

  • Effects of ascorbate on membrane phospholipids and tocopherols of intact erythrocytes during peroxidation by t-butylhydroperoxide: comparison with effects of dithiothreitol.

    abstract::Peroxidation of intact human erythrocytes by t-butylhydroperoxide (tBHP)) was studied. By incubation of the erythrocytes with 1 mM tBHP, reduced glutathione (GSH) was exhausted within 1 min, and tocopherols (Toc) and phospholipids (PL) decreased to nearly their lowest levels (in this study) within 5 min. The rate of d...


    pub_type: 杂志文章


    authors: Mawatari S,Murakami K

    更新日期:2001-01-01 00:00:00

  • Desaturation indices in liver, muscle, and bone of growing male and female mice fed trans-10,cis-12 conjugated linoleic acid.

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    pub_type: 杂志文章


    authors: Viswanadha S,McGilliard ML,Herbein JH

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  • Lipid peroxidation in rat tissue homogenates: Interaction of iron and ascorbic acid as the normal catalytic mechanism.

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    pub_type: 杂志文章


    authors: Barber AA

    更新日期:1966-03-01 00:00:00

  • The metabolism of malondialdehyde.

    abstract::Interest in malondialdehyde (MDA) metabolism stems from its formation as a product of lipid peroxidation in the diet and in the tissues; its reactivity with functional groups of nucleic acid bases, proteins and phospholipids; its mutagenicity in bacteria, and its reported skin and liver carcinogenicity in animals. Adm...


    pub_type: 杂志文章


    authors: Draper HH,McGirr LG,Hadley M

    更新日期:1986-04-01 00:00:00

  • Plasma fatty acids and lipids in two separate, but genetically comparable, Icelandic populations.

    abstract::Levels of serum lipids and lipoproteins, and the fatty acid composition of plasma phospholipids, were measured in two genetically comparable, but widely separated, populations. The 1975 mortality rates for ischemic heart disease were significantly higher in one of these populations, the Manitoban residents of pure Ice...


    pub_type: 杂志文章


    authors: Skúladóttir GV,Gudmundsdóttir S,Olafsson GB,Sigurdsson SB,Sigfússon N,Axelsson J

    更新日期:1995-07-01 00:00:00

  • Effect of peanut oil and randomized peanut oil on cholesterol and oleic acid absorption, transport, and distribution in the lymph of the rat.

    abstract::Peanut oil was shown to be atherogenic in cholesterol-fed rats, rabbits, and monkeys. However, after randomization, a process in which the fatty acids in peanut oil are randomly rearranged, its atherogenicity was significantly reduced in cholesterol-fed rabbits and monkeys. The mechanism for this effect remains unknow...


    pub_type: 杂志文章


    authors: Satchithanandam S,Flynn TJ,Calvert RJ,Kritchevsky D

    更新日期:1999-12-01 00:00:00

  • Effect of dietary linseed oil on tumoricidal activity and eicosanoid production in murine macrophages.

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    pub_type: 杂志文章


    authors: Hubbard NE,Chapkin RS,Erickson KL

    更新日期:1994-09-01 00:00:00

  • Separation and quantitation of mono-, di-, and triglycerides and free oleic acid using thin-layer chromatography with flame-ionization detection.

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    pub_type: 杂志文章


    authors: Peyrou G,Rakotondrazafy V,Mouloungui Z,Gaset A

    更新日期:1996-01-01 00:00:00

  • Studies of triacyglycerol structure of very low density lipoproteins of normolipemic subjects and patients with type III and type IV hyperlipoproteinemia.

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    pub_type: 杂志文章


    authors: Myher JJ,Kuksis A,Breckenridge WC,Little JA

    更新日期:1984-09-01 00:00:00

  • Relationship Between Circulating Fatty Acids and Fatty Acid Ethanolamide Levels After a Single 2-h Dietary Fat Feeding in Male Sprague-Dawley Rats : Elevated levels of oleoylethanolamide, palmitoylethanolamide, linoleoylethanolamide, arachidonoylethanolam

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    pub_type: 杂志文章


    authors: Olatinsu AO,Sihag J,Jones PJH

    更新日期:2017-11-01 00:00:00

  • Ketone bodies, glucose and glutamine as lipogenic precursors in human diploid fibroblasts.

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    pub_type: 杂志文章


    authors: Reed WD,Zielke HR,Baab PJ,Ozand PT

    更新日期:1981-09-01 00:00:00

  • Thealpha-oxidation system of brain microsomes. Cofactors foralpha-hydroxy acid decarboxylation.

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    pub_type: 杂志文章


    authors: Macdonald RC,Mead JF

    更新日期:1968-05-01 00:00:00

  • The effect of neurotensin on the concentration of cholesterol and bile acids in the guinea pig.

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    pub_type: 杂志文章


    authors: Peric-Golia L,Gardner CF,Wilson DE,Peric-Golia M

    更新日期:1984-10-01 00:00:00

  • Alveolar lavage and lavaged lung tissue phosphatidylcholine composition during fetal rabbit development.

    abstract::Pulmonary surfactant, the major surface-active component of which in the adult is dipalmitoylglycerophosphocholine, can be obtained by lavaging lungs with physiological saline. We have previously shown that there is an increase in the amount of phosphatidylcholine in fetal rabbit lung lavage during the latter part of ...


    pub_type: 杂志文章


    authors: Rooney SA,Gobran LI

    更新日期:1977-12-01 00:00:00

  • The effect of n-3 fatty acids on coronary atherosclerosis: results from SCIMO, an angiographic study, background and implications.

    abstract::According to the model of "response to injury," the arterial endothelium is occasionally injured in hyperlipidemia, hypertension, diabetes mellitus and in other states known as risk factors. The ensuing inflammatory response is modulated by cytokines and growth factors, among them platelet-derived growth factor (PDGF)...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: von Schacky C,Baumann K,Angerer P

    更新日期:2001-01-01 00:00:00

  • Adipose monoacylglycerol:acyl-coenzyme A acyltransferase activity in the white-throated sparrow (Zonotrichia albicollis): characterization and function in a migratory bird.

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    pub_type: 杂志文章


    authors: Mostafa N,Bhat BG,Coleman RA

    更新日期:1994-11-01 00:00:00

  • Cytotoxic effect of conjugated trienoic fatty acids on mouse tumor and human monocytic leukemia cells.

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    pub_type: 杂志文章


    authors: Suzuki R,Noguchi R,Ota T,Abe M,Miyashita K,Kawada T

    更新日期:2001-05-01 00:00:00