Deoxycholic acid formation in gnotobiotic mice associated with human intestinal bacteria.

Abstract:

:In humans and animals, intestinal flora is indispensable for bile acid transformation. The goal of our study was to establish gnotobiotic mice with intestinal bacteria of human origin in order to examine the role of intestinal bacteria in the transformation of bile acids in vivo using the technique of gnotobiology. Eight strains of bile acid-deconjugating bacteria were isolated from ex-germ-free mice inoculated with a human fecal dilution of 10(-6), and five strains of 7alpha-dehydroxylating bacteria were isolated from the intestine of limited human flora mice inoculated only with clostridia. The results of biochemical tests and 16S rDNA sequence analysis showed that seven out of eight bile acid-deconjugating strains belong to a bacteroides cluster (Bacteroides vulgatus, B. distasonis, and B. uniformis), and one strain had high similarity with Bilophila wadsworthia. All five strains that converted cholic acid to deoxycholic acid had greatest similarity with Clostridium hylemonae. A combination of 10 isolated strains converted taurocholic acid into deoxycholic acid both in vitro and in the mouse intestine. These results indicate that the predominant bacteria, mainly Bacteroides, in human feces comprise one of the main bacterial groups for the deconjugation of bile acids, and clostridia may play an important role in 7aplha-dehydroxylation of free-form primary bile acids in the intestine although these strains are not predominant. The gnotobiotic mouse with bacteria of human origin could be a useful model in studies of bile acid metabolism by human intestinal bacteria in vivo.

journal_name

Lipids

journal_title

Lipids

authors

Narushima S,Itoha K,Miyamoto Y,Park SH,Nagata K,Kuruma K,Uchida K

doi

10.1007/s11745-006-5038-1

subject

Has Abstract

pub_date

2006-09-01 00:00:00

pages

835-43

issue

9

eissn

0024-4201

issn

1558-9307

journal_volume

41

pub_type

杂志文章

相关文献

LIPIDS文献大全
  • Conversion of linoleic acid hydroperoxide by soybean lipoxygenase in the presence of guaiacol: identification of the reaction products.

    abstract::Linoleic acid hydroperoxide formed by soybean lipoxygenase was metabolized by the same enzyme in the presence of guaiacol. The products of this reaction included, trihydroxyoctadecenoic acids, hydroperoxydihydroxyoctadecenoic acids, hydroxyepoxyoctadecenoic acids, dihydroxyoctadecenoic acids, hydroxyoctadecadienoic ac...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02532331

    authors: Streckert G,Stan HJ

    更新日期:1975-12-01 00:00:00

  • Effects of margarine and butter consumption on distribution of trans-18:1 fatty acid isomers and conjugated linoleic acid in major serum lipid classes in lactating women.

    abstract::Trans FA (TFA) have at least one trans double bond and comprise several isomers and types, including many of the CLA (e.g., c9,t11-18:2 CLA). Some TFA may have adverse effects (e.g., cardiovascular disease), whereas some are thought to have beneficial effects (e.g., anticarcinogenicity). The presence of TFA in human t...

    journal_title:Lipids

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s11745-006-5082-x

    authors: Shahin AM,McGuire MK,Anderson N,Williams J,McGuire MA

    更新日期:2006-02-01 00:00:00

  • Effect of lecithin on the release of 5'-nucleotidase from liver plasma membrane of rat by bile acids.

    abstract::Bile acids solubilize proteins from liver plasma membrane both in vivo and in vitro. The ability to solubilize the proteins is dependent on the species of bile acid. In this paper, the effect of phospholipid on the solubilization of a membrane-bound enzyme by bile acids was investigated in vitro. Taurocholate (TC) and...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534873

    authors: Nokubo M

    更新日期:1987-01-01 00:00:00

  • An in vitro method for studying the proliferation and differentiation of Atlantic salmon preadipocytes.

    abstract::The aim of the present study was to develop a cell culture system for studying the proliferation and differentiation of preadipocytes isolated from Atlantic salmon adipose tissue. The expression of proliferating cell nuclear antigen (PCNA) was used as a marker for cell proliferation. The cells started to proliferate w...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-003-1063-3

    authors: Vegusdal A,Sundvold H,Gjøen T,Ruyter B

    更新日期:2003-03-01 00:00:00

  • Evaluation of apolipoprotein A-I kinetics in rabbits in vivo using in situ and exogenous radioiodination methods.

    abstract::The kinetics of in vivo clearance of apolipoprotein (apo) A-I radioiodinated by the iodine monochloride (ICI) method of McFarlane [McFarlane, A.S. (1958) Efficient Trace-Labelling of Proteins with Iodine, Nature 182, 53] as modified by Bilheimer and co-workers [Bilheimer, D.W., Eisenberg, S., and Levy, R.I. (1972) The...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-003-1053-5

    authors: Zhang B,Shimoji E,Tanaka H,Saku K

    更新日期:2003-03-01 00:00:00

  • Enteral absorption in man of eicosapentaenoic acid in different chemical forms.

    abstract::After administering the equivalent of 1 g of eicosapentaenoic acid (EPA) in four different chemical forms, the kinetics of EPA incorporation into plasma triglycerides (TG) were compared by gas liquid chromatography on a capillary column following separation of the lipid fraction by thin layer chromatography. EPA incor...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533970

    authors: el Boustani S,Colette C,Monnier L,Descomps B,Crastes de Paulet A,Mendy F

    更新日期:1987-10-01 00:00:00

  • Triolein and trilinolein ameliorate oxidized low-density lipoprotein-induced oxidative stress in endothelial cells.

    abstract::Uptake of oxidized low-density lipoprotein by endothelial cells is a critical step for the initiation of atherosclerosis. Triacylglycerol uptake in these cells is understood to be a part of the process. The present investigation, comparison among the effects of simple acylglycerol, including tristearin, triolein, and ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-014-3889-4

    authors: Luo T,Deng ZY,Li XP,Rao H,Fan YW

    更新日期:2014-05-01 00:00:00

  • Composition of lipids of bovine optic nerve.

    abstract::Lipids from bovine optic nerve were analyzed. The total content of 16.5% by weight included 27.2% nonpolar lipids, 26.1% glycolipids, and 46.7% phospholipids by weight. Free cholesterol was the major component of the nonpolar lipid fraction. The cerebrosides, 73.5% of total glycolipids, were separated by thin layer ch...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533745

    authors: Das SK,Steen ME,McCullough MS,Bhattacharyya DK

    更新日期:1978-10-01 00:00:00

  • Interconversion of fatty aldehydes and dimethyl acetals at low temperatures.

    abstract::Facile procedures are described for nearly quantitative conversion of saturated and unsaturated fatty aldehydes to their dimethyl acetals, and vice versa, at low temperatures. The methods are based on the chemical behavior of aldehydes and dimethyl acetals in 100% sulfuric acid. Under the experimental conditions descr...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02532679

    authors: Mahadevan V,Viswanathan CV,Lundberg WO

    更新日期:1966-09-01 00:00:00

  • Tumor necrosis factor-alpha and ceramides in insulin resistance.

    abstract::The present studies tested the hypothesis that some effects of tumor necrosis factor-alpha (TNF-alpha) are mediated by activation of sphingomyelinases and the production of ceramides. Differentiated 3T3-L1 adipocytes were incubated with short-chain ceramide analogs, (C2- and C6-ceramides: N-acetyl- and N-hexanoyl-sphi...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02562240

    authors: Brindley DN,Wang CN,Mei J,Xu J,Hanna AN

    更新日期:1999-01-01 00:00:00

  • Detection of Endogenous Phosphatidylinositol 4,5-bisphosphate in Phytophthora cinnamomi.

    abstract::Plant diseases caused by Phytophthora species are serious threats to agriculture and the natural environment. Genome sequencing has revealed the lack of a gene for canonical phospholipase C (PLC), an enzyme that was hitherto thought to be ubiquitous in eukaryotes. PLC acts in the hydrolysis of phosphatidylinositol 4,5...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1002/lipd.12093

    authors: Howard JM,Beech PL,Callahan DL

    更新日期:2018-08-01 00:00:00

  • Difficulties in the assay of phosphatidate phosphohydrolase activity. Influence of ionic strength, detergent, and selection of substrate.

    abstract::In the present paper, problems in connection with assay of the activity of magnesium-dependent rat liver phosphatidate phosphohydrolase (PAP) are discussed. PAP activity is usually measured by following the production of diacylglycerol or inorganic phosphate from the substrate phosphatidate. These two methods may give...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02522468

    authors: Stark M,Humble E

    更新日期:1996-10-01 00:00:00

  • Effect of dietary fat on the lipid composition and utilization of short-chain fatty acids by rat colonocytes.

    abstract::The objective of the present studies was to examine the effect of dietary fat on the lipid composition of rat colonocytes and their utilization of short-chain fatty acids (SCFA). Rats were fed 14% beef fat, fish oil or safflower oil plus 2% corn oil in a semi-synthetic base diet for 4 wk. Colonocytes were isolated and...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02544340

    authors: Awad AB,Ferger SL,Fink CS

    更新日期:1990-06-01 00:00:00

  • pH gradient electrophoresis and isoelectric focusing of lipoproteins on agarose bead thin layers.

    abstract::A new method of isoelectric focusing (IEF) and pH gradient electrophoresis, using thin layers of agarose gel beads, was devised to investigate chylomicrons and very low density lipoproteins (VLDL). pH gradient stability and cathodal gradient drift were similar to those of polyacrylamide gel IEF, and linearity of gradi...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534130

    authors: Vost A,Pocock DM,Pleet S

    更新日期:1979-10-01 00:00:00

  • Response of plasma lipids to dietary cholesterol and wine polyphenols in rats fed polyunsaturated fat diets.

    abstract::This experiment was designed to evaluate the effects of dietary red wine phenolic compounds (WP) and cholesterol on lipid oxidation and transport in rats. For 5 wk, weanling rats were fed polyunsaturated fat diets (n-6/n-3 = 6.4) supplemented or not supplemented with either 3 g/kg diet of cholesterol, 5 g/kg diet of W...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-000-0610-2

    authors: Frémont L,Gozzelino MT,Linard A

    更新日期:2000-09-01 00:00:00

  • Effects of high-gamma-linolenic acid canola oil compared with borage oil on reproduction, growth, and brain and behavioral development in mice.

    abstract::Previous research in rats and mice has suggested that gamma-linolenic acid (GLA) derived from borage oil (BO: 23% GLA) may be an appropriate source for increasing levels of long-chain n-6 FA in the developing brain. Recently, transgenic technology has made available a highly enriched GLA seed oil from the canola plant...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-003-1048-2

    authors: Wainwright PE,Huang YS,DeMichele SJ,Xing H,Liu JW,Chuang LT,Biederman J

    更新日期:2003-02-01 00:00:00

  • Lysophosphatidylcholine acyltransferase activity in Saccharomyces cerevisiae: regulation by a high-affinity Zn2+ binding site.

    abstract::Saccharomyces cerevisiae cells were demonstrated to contain lysophosphatidylcholine (lysoPtdCho) acyltransferase (E.C. 2.3.1.23) activity. The enzyme displayed Km(app) of 69 microM for lysoPtdCho and 152 microM for oleoyl CoA. Enzyme activity was not affected by the addition of 1 mM Mg2+, Mn2+, Ca2+, or 200 mM EDTA. H...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-998-0328-1

    authors: Richard MG,McMaster CR

    更新日期:1998-12-01 00:00:00

  • Lipopolysaccharide Alters Thermogenic and Inflammatory Genes in White Adipose Tissue in Mice Fed Diets with Distinct 18-Carbon Fatty-Acid Composition.

    abstract::Fatty acids are involved in the regulation of inflammatory responses in obesity. However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied. Therefore, the objective of...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1002/lipd.12101

    authors: Shin S,Ajuwon KM

    更新日期:2018-09-01 00:00:00

  • Dietary docosahexaenoic acid and immunocompetence in young healthy men.

    abstract::The purpose of this study was to examine the effect of dietary docosahexaenoic acid (DHA), in the absence of eicosapentaenoic acid, on human immune response (IR). A 120-d study with 11 healthy men was conducted at the Metabolic Research Unit of the Western Human Nutrition Research Center. Four subjects (control group)...

    journal_title:Lipids

    pub_type: 临床试验,杂志文章

    doi:10.1007/s11745-998-0240-8

    authors: Kelley DS,Taylor PC,Nelson GJ,Mackey BE

    更新日期:1998-06-01 00:00:00

  • Hydrocarbon chain distribution of ether phospholipids of the ascidian Halocynthia roretzi and the sea urchin Strongylocentrotus intermedius.

    abstract::The contents and compositions of the 1-O-alk-1'-enyl-2-acyl, 1-O-alkyl-2-acyl, and 1,2-diacyl glycerophospholipids in the muscle and viscera of the ascidian Halocynthia roretzi, and of the gonad of the sea urchin Strongylocentrotus intermedius, which are eaten to some extent in Alaska and in Asia, were analyzed by gas...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02522404

    authors: Jeong BY,Ohshima T,Koizumi C

    更新日期:1996-01-01 00:00:00

  • Biochemical and physiological effects of sterol alterations in yeast--a review.

    abstract::Considerable progress has been made in the selection and characterization of mutants that are defective in the synthesis of ergosterol in the yeast, Saccharomyces cerevisiae. Mutations in nearly every step of the yeast sterol biosynthetic pathway have been induced and selected. These mutants have been used to elucidat...

    journal_title:Lipids

    pub_type: 杂志文章,评审

    doi:10.1007/BF02537825

    authors: Parks LW,Smith SJ,Crowley JH

    更新日期:1995-03-01 00:00:00

  • Synthesis of octadecynoic acids and [1-14C] labeled isomers of octadecenoic acids.

    abstract::Geometric and positional isomers of [1-14C] octadecenoic acids have been synthesized by modifications of published procedures. Positional isomers of octadecynoic acids also have been synthesized to obtain the geometric and positional isomers of the unlabeled octadecenoic acid analogs. The syntheses were accomplished b...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534194

    authors: Valicenti AJ,Pusch FJ,Holman RT

    更新日期:1985-04-01 00:00:00

  • Development of an LC-MS/MS method for the analysis of free sphingoid bases using 4-fluoro-7-nitrobenzofurazan (NBD-F).

    abstract::The molecular species of sphingoid bases were tagged with the fluorescent amino group reagent, 4-fluoro-7-nitrobenzofurazan (NBD-F). The NBD-sphingoid bases were analyzed by a highly selective and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) technique capable of relia...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-013-3871-6

    authors: Ishikawa T,Imai H,Maki KY

    更新日期:2014-03-01 00:00:00

  • Serum high density lipoprotein and its relationship to cardiovascular disease risk factor variables in children--the Bogalusa heart study.

    abstract::Serum high density lipoprotein is increasingly recognized as a negative risk for cardiovascular disease. The distribution and interrelationship of serum lipids, lipoproteins, anthropometric measurements and blood pressures were determined in some 5,000 children. Children had mean +/- S.D. alpha-lipoprotein cholesterol...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533576

    authors: Berenson GS,Srinivasan SR,Frerichs RR,Webber LS

    更新日期:1979-01-01 00:00:00

  • Quantification of phospholipids classes in human milk.

    abstract::Phospholipids are integral constituents of the milk fat globule membranes and they play a central role in infants' immune and inflammatory responses. A methodology employing liquid chromatography coupled with evaporative light scattering detector has been optimized and validated to quantify the major phospholipids cla...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-013-3825-z

    authors: Giuffrida F,Cruz-Hernandez C,Flück B,Tavazzi I,Thakkar SK,Destaillats F,Braun M

    更新日期:2013-10-01 00:00:00

  • High Sucrose Intake Ameliorates the Accumulation of Hepatic Triacylglycerol Promoted by Restraint Stress in Young Rats.

    abstract::Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disorder. Stress promotes the onset of the NAFLD with a concomitant increment in the activity of the hepatic 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD-1). However, the interaction between the stress and a carbohydrate-enriched diet for t...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-015-4066-0

    authors: Corona-Pérez A,Díaz-Muñoz M,Rodríguez IS,Cuevas E,Martínez-Gómez M,Castelán F,Rodríguez-Antolín J,Nicolás-Toledo L

    更新日期:2015-11-01 00:00:00

  • Effect of acute ethanol ingestion on fat absorption.

    abstract::A test meal (300 mg casein, 600 mg sucrose, 100 mg corn oil, tracer dose of 9.10(3)H oleic acid) was given to fasting adult rats with intestinal lymph fistulas. One group received an acute oral dose of ethanol (3.2 g/kg body weight) simultaneously with the test meal. Controls received 2.5 ml of water instead of ethano...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02532991

    authors: Boquillon M

    更新日期:1976-12-01 00:00:00

  • Comparative intestinal and colonic absorption of [4-14C] cholesterol in the rat.

    abstract::[4(-14)C] Cholesterol was administered as an aqueous emulsion with triolein and dry non-fat milk either directly into the upper duodenum or into the ileocaecal junction of lymph duct cannulated rats. Lymph flow rates were similar in the two groups of animals. Whereas ca. 53% of the administered tracer dose of choleste...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533375

    authors: Roy T,Treadwell CR,Vahouny GV

    更新日期:1978-01-01 00:00:00

  • Highly unsaturated fatty acid synthesis in marine fish: cloning, functional characterization, and nutritional regulation of fatty acyl delta 6 desaturase of Atlantic cod (Gadus morhua L.).

    abstract::This study reports the cloning, functional characterization, tissue expression, and nutritional regulation of a delta6 fatty acyl desaturase of Atlantic cod (Gadus morhua). PCR primers were designed based on the sequences of conserved motifs in available fish desaturases and used to isolate a cDNA fragment from cod li...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-006-5051-4

    authors: Tocher DR,Zheng X,Schlechtriem C,Hastings N,Dick JR,Teale AJ

    更新日期:2006-11-01 00:00:00

  • The effect of short-term lipid infusion on liver function and biliary secretion in rats.

    abstract::This study was undertaken to determine the effect of various lipid emulsions on the hepato-biliary system in rats. Rats were randomly divided into six groups and infused continuously for 48 hr with either long-chain triglycerides (LCT), medium-chain triglycerides (MCT) or a mixture of MCT and LCT. One group infused wi...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02536144

    authors: Rubin M,Halpern Z,Livoff A,Wennberg A,Tietz A,Antebi E,Lichtenberg D

    更新日期:1992-05-01 00:00:00