Abstract：
:Microtiter plate methods were developed for the enzymatic determination of serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and triglyceride (TG), and for the turbidometric determination of apolipoproteins. The micromethods resulted in accurate, precise values that were in good agreement with the conventional spectrophotometric assays. The coefficient of variation for TC determinations was 4.5% or less and bias was 5% or less. The lipid micromethod assays are sensitive to 10 mg/dL or less, and the apolipoprotein assay to 1 mg/dL. Less than 100 microL of serum suffices for TC, TG and apoprotein assays; HDL-C requires an additional 100 microL of serum. Advantages of the micromethods include reductions in assay time and in the amount of reagents required.
journal_name
Lipidsjournal_title
Lipidsauthors
Shireman RB,Durieux Jdoi
10.1007/BF02535780subject
Has Abstractpub_date
1993-02-01 00:00:00pages
151-5issue
2eissn
0024-4201issn
1558-9307journal_volume
28pub_type
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