Two distinct pathways for the formation of hydroxy FA from linoleic acid by lactic acid bacteria.

Abstract:

:Twenty-three of 86 strains of lactic acid bacteria transformed linoleic acid into hydroxy FA. Two distinct conversion pathways were in operation. Two strains of Lactobacillus acidophilus and a strain of Pediococcus pentosaceus produced 13(S)-hydroxy-9-octadecenoic acid 113(S)-OH 18:11 and 10,13dihydroxyoctadecanoic acid (10,13-OH 18:0) as main and minor products, respectively, whereas 13 strains, including L. casei subsp. casei, L. paracasei subsp. paracasei, L. rhamnosus, L. lactis subsp. cremoris, and Streptococcus salivarius subsp. thermophilus produced 10-hydroxy-12-octadecenoic acid (10-OH 18:1). Seven strains of L. plantarum converted linoleic acid to 10-hydroxyoctadecanoic acid (10-OH 18:0) through 10-OH 18:1. Linoleic acid at 2 g/L was converted by L. acidophilus IFO13951T to 1.3 g of 13(S)-OH 18:1 and 0.09 g of 10,13-OH 18:0 in 7 d. Lactobacillus paracasei subsp. paracasei JCM 1111 produced 10-OH 18:1 in 91% yield, and L. plantarum JCM 8341, 10-OH 18:0 in 59% yield from linoleic acid (2 g/L) under optimal conditions. To our knowledge, this is the first report on the production of 13(S)-OH 18:1 by lactic acid bacteria other than ruminal bacteria, and of 10,13-OH 18:0 by any bacteria.

journal_name

Lipids

journal_title

Lipids

authors

Kishimoto N,Yamamoto I,Toraishi K,Yoshioka S,Saito K,Masuda H,Fujita T

doi

10.1007/s11745-003-1188-4

subject

Has Abstract

pub_date

2003-12-01 00:00:00

pages

1269-74

issue

12

eissn

0024-4201

issn

1558-9307

journal_volume

38

pub_type

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