Quantitative 31P nuclear magnetic resonance analysis of the phospholipids of erythrocyte membranes using detergent.

abstract：:Primary cultured hepatocytes from normal mice and mice with Sarcoma 180 were characterized. The viability of freshly isolated hepatocytes from both sources was over 90% and the cells had a relatively stable population of DNA for a minimum of three days. After incubation with (3H)leucine, the syntheses and secretions o...

journal_title：Lipids

authors： Masuno H,Okuda H

更新日期：1985-07-01 00:00:00

abstract：:After administering the equivalent of 1 g of eicosapentaenoic acid (EPA) in four different chemical forms, the kinetics of EPA incorporation into plasma triglycerides (TG) were compared by gas liquid chromatography on a capillary column following separation of the lipid fraction by thin layer chromatography. EPA incor...

journal_title：Lipids

authors： el Boustani S,Colette C,Monnier L,Descomps B,Crastes de Paulet A,Mendy F

更新日期：1987-10-01 00:00:00

abstract：:Docosahexaenoic acid (DHA), a prominent long-chain fatty acid of the omega-3 family, is present at high amount in brain tissues, especially in membrane phospholipids. This polyunsaturated fatty acid is the precursor of various oxygenated lipid mediators involved in diverse physiological and pathophysiological processe...

journal_title：Lipids

authors： Jouvène C,Fourmaux B,Géloën A,Balas L,Durand T,Lagarde M,Létisse M,Guichardant M

更新日期：2018-01-01 00:00:00

abstract：:Recently, lipases have been purified from mammalian, bacterial, fungal and plant sources by different methodologies. Purified lipases subsequently have been characterized for molecular size, metal binding capabilities, glycoside and phosphorus contents, and substrate specificities. Primary structures of several lipase...

journal_title：Lipids

authors： Antonian E

更新日期：1988-12-01 00:00:00

abstract：:The effects of 2-hexadecynoic acid on the growth and lipid metabolism of cultured 7288 (HTC) cells have been evaluated. Growth was inhibited by the acetylenic acid: the LD50 was 35-85 microM as determined by two methods at low and high cell densities. Reduced growth did not result from damaged plasma membranes as dete...

journal_title：Lipids

authors： Upreti GC,Matocha M,Wood R

更新日期：1981-05-01 00:00:00

abstract：:Total lipid content in the thermophilic fungi-Thermoascus aurantiacus, Humicola lanuginosa, Malbranchea pulchella var.sulfurea, andAbsidia ramosa-varied from 5.3 to 19.1% of mycelial dry weight. The neutral and polar lipid fractions accounted for 56.4 to 80.2% and 19.8 to 43.6%, respectively. All the fungi contained m...

journal_title：Lipids

authors： Raju KS,Maheshwari R,Sastry PS

更新日期：1976-10-01 00:00:00

abstract：:We describe a method for the quantitative analysis of molecular species of diacylglycerol and alkylacylglycerol as their diradylglycerobenzoate derivatives. Synthetic internal standards were used to provide quantitative determinations of the low levels of diacylglycerol and alkylacylglycerol and their individual molec...

journal_title：Lipids

authors： Warne TR,Robinson M

更新日期：1990-11-01 00:00:00

abstract：:The source of free fatty acids (FFA) and the pathways contributing to the accumulation of neutral fats in livers of rats fed a cholesterol-enriched diet were investigated in this report. Supplementation with 1% cholesterol in the diet for four weeks resulted in hepatomegaly in the rats. The contents of cholesterol and...

journal_title：Lipids

authors： Liu CH,Huang MT,Huang PC

更新日期：1995-06-01 00:00:00

abstract：:The effects of droplet size and emulsifiers on oxidative stability of polyunsaturated TAG in oil-in-water (o/w) emulsions with droplet sizes of 0.806 +/- 0.0690, 3.28 +/- 0.0660, or 10.7 +/- 0.106 microm (mean +/- SD) were investigated. Hydroperoxide contents in the emulsion with a mean droplet size of 0.831 microm we...

journal_title：Lipids

authors： Nakaya K,Ushio H,Matsukawa S,Shimizu M,Ohshima T

更新日期：2005-05-01 00:00:00

abstract：:This work reports the effect of the apolipoproteins A-I and A-II (apoA-I and apoA-II) on the release of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-8, and IL-1 receptor antagonist (IL-1Ra) and on the oxidative burst of human neutrophils. By themselves, apoA-I and apoA-II do not affect the basal liber...

journal_title：Lipids

authors： Furlaneto CJ,Ribeiro FP,Hatanaka E,Souza GM,Cassatella MA,Campa A

更新日期：2002-09-01 00:00:00

abstract：:Cholesterol itself has very few structural/chemical features suitable for real-time imaging in living cells. Thus, the advent of dehydroergosterol [ergosta-5,7,9(11),22-tetraen-3beta-ol, DHE] the fluorescent sterol most structurally and functionally similar to cholesterol to date, has proven to be a major asset for re...

journal_title：Lipids

authors： McIntosh AL,Atshaves BP,Huang H,Gallegos AM,Kier AB,Schroeder F

更新日期：2008-12-01 00:00:00

abstract：:Tris(N,N-dimethyl)amide of phosphorous acid activated by addition of iodine at an optimal molar ratio of 1.05:0.05 was used as a phosphorylating reagent to synthesize cholecalciferyl-3-O-(1-2-isopropylidene-rac-glycero-3-O-)-thiophos phate and cholecalciferyl-3-O-(1,2-dipalmitoyl-rac-glycero-3-O-)thiophosphat e of met...

journal_title：Lipids

authors： Stamatov SD,Gronowitz S

更新日期：1992-06-01 00:00:00

abstract：:The contents and compositions of the 1-O-alk-1'-enyl-2-acyl, 1-O-alkyl-2-acyl, and 1,2-diacyl glycerophospholipids in the muscle and viscera of the ascidian Halocynthia roretzi, and of the gonad of the sea urchin Strongylocentrotus intermedius, which are eaten to some extent in Alaska and in Asia, were analyzed by gas...

journal_title：Lipids

authors： Jeong BY,Ohshima T,Koizumi C

更新日期：1996-01-01 00:00:00

abstract：:By in vivo and in vitro studies of L-(3-3H)serine and [9,10(n)-3H]palmitic acid incorporation into phospholipids, we show a change in the renewal of the ceramide moiety of sphingomyelin in the gills of euryhaline fish (sea bass and eels) when the animals were subjected to abrupt alterations in environmental salinity. ...

journal_title：Lipids

authors： el Babili M,Brichon G,Zwingelstein G

更新日期：1996-04-01 00:00:00

abstract：:Potato tuber lipoxygenase (ptLOX) has been shown to catalyze the aerobic formation of at least four major oxygenated derivatives of DHA. Two of the products--7,17(S)- and 10,17(S)-dihydro(pero)xy-DHA [7,17- and 10,17-diH(P)DHA]--were formed from soybean 15-LOX-derived 17(S)-hydro(pero)xy-DHA [17(S)-H(P)DHA], whereas t...

journal_title：Lipids

authors： Butovich IA,Hamberg M,Rådmark O

更新日期：2005-03-01 00:00:00

abstract：:Levels of serum lipids and lipoproteins, and the fatty acid composition of plasma phospholipids, were measured in two genetically comparable, but widely separated, populations. The 1975 mortality rates for ischemic heart disease were significantly higher in one of these populations, the Manitoban residents of pure Ice...

journal_title：Lipids

authors： Skúladóttir GV,Gudmundsdóttir S,Olafsson GB,Sigurdsson SB,Sigfússon N,Axelsson J

更新日期：1995-07-01 00:00:00

abstract：:A protocol, based on the use of Pseudomonas lipase, is presented to measure quantitatively the amount of triacylglycerols in extracts from cultured cells of tissues. Since the lipase also acts on di- and monoacylglycerols, separation of the extracts by thin-layer chromatography is recommended. In order to allow the li...

journal_title：Lipids

authors： Van Veldhoven PP,Swinnen JV,Esquenet M,Verhoeven G

更新日期：1997-12-01 00:00:00

abstract：:The liver lipogenic enzymes are compared among rats, chickens, frogs and fish. Although the apparent Km values of glucose-6-phosphate dehydrogenase for glucose-6-phosphate are not much different among all the species, those of malic enzyme for malate are much higher in chickens and fish than in rats and frogs. Glucose...

journal_title：Lipids

authors： Iritani N,Ikeda Y,Fukuda H,Katsurada A

更新日期：1984-11-01 00:00:00

abstract：:The purpose of the present study was to investigate the effects of gamma linolenic acid (GLA) on cardiovascular responses to psychosocial stress (isolation) and to pressor hormones in the genetically borderline hypertensive rat (SHR X WKY). Adult male SHR X WKY were divided into two groups following five weeks of grou...

journal_title：Lipids

authors： Mills DE,Summers MR,Ward RP

更新日期：1985-09-01 00:00:00

abstract：:The molecular species of sphingoid bases were tagged with the fluorescent amino group reagent, 4-fluoro-7-nitrobenzofurazan (NBD-F). The NBD-sphingoid bases were analyzed by a highly selective and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) technique capable of relia...

journal_title：Lipids

authors： Ishikawa T,Imai H,Maki KY

更新日期：2014-03-01 00:00:00

abstract：:The acylation of lysophospholipids by rat liver acyltransferases was studied. A comparison between ester and ether lysophospholipids as substrates revealed large differences in substrate properties. For instance, oleic acid from oleoyl-CoA and arachidonic acid from arachidonoyl-CoA were not incorporated into 1-O-octad...

journal_title：Lipids

authors： Neumüller W,Fleer EA,Unger C,Eibl H

更新日期：1987-11-01 00:00:00

abstract：:An important enzyme involved in phospholipid turnover is the acyl-CoA: lysophosphatidylcholine acyltransferase (LPCAT). Here, we report characterization of a newly discovered human LPCAT (LPCAT3), which has distinct substrate preferences strikingly consistent with a role in phosphatidylcholine (PtdCho) remodeling and ...

journal_title：Lipids

authors： Kazachkov M,Chen Q,Wang L,Zou J

更新日期：2008-10-01 00:00:00

abstract：:The phospholipid composition of gill tissue from thermally acclimated rainbow trout, Salmo gairdneri, was determined by Iatroscan analysis following an initial development of the chromarods in a non-polar solvent to remove neutral lipids. Standard curves for all phospholipids, although linear through most of the conce...

journal_title：Lipids

authors： Hazel JR

更新日期：1985-08-01 00:00:00

abstract：:CLA inhibits mammary cancer and reduces body fat accumulation in rodents. It is not known whether uncoupling proteins (UCP), which are modulators of energy balance and metabolism, play a role in these actions of CLA. To determine the effects of dietary CLA on the expression of UCP in various tissues, 5-wk-old Sprague-...

journal_title：Lipids

authors： Ealey KN,El-Sohemy A,Archer MC

更新日期：2002-09-01 00:00:00

abstract：:The incorporation of arachidonic acid (AA) as compared to eicosapentaenoic acid (EPA) into human platelet phospholipids was tested by incubating washed platelets with a known mixture of [3H]AA and [14C]EPA. Following incubation, the platelet lipids were extracted, the individual phospholipids--phosphatidylcholine (PC)...

journal_title：Lipids

authors： Weaver BJ,Holub BJ

更新日期：1985-11-01 00:00:00

abstract：:A one-carbon degradation of long-chain fatty acids, which was found to occur in the brains of rats in vivo, has been investigated in a brain microsomal fraction in vitro. Decarboxylation of the alpha-hydroxy acid, a possible intermediate product between the substrate and the next shorter acid, in the presence of brain...

journal_title：Lipids

authors： Macdonald RC,Mead JF

更新日期：1968-05-01 00:00:00

abstract：:Reproductive tissues from male house and field crickets were investigated for the presence of arachidonic acid. Arachidonic acid was identified by retention behavior on 3 gas liquid chromatographic column systems-SE-30, OV 225, and Silar 10C-and by gas liquid/positive ion chemical ionization mass spectrometry. Arachid...

journal_title：Lipids

authors： Worthington RE,Brady UE,Thean JE,Wilson DM

更新日期：1981-01-01 00:00:00

abstract：:Fatty acids are involved in the regulation of inflammatory responses in obesity. However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied. Therefore, the objective of...

journal_title：Lipids

authors： Shin S,Ajuwon KM

更新日期：2018-09-01 00:00:00

abstract：:The tissue distribution of newly synthesized 22:6n-3 and intermediate PUFA was examined in rainbow trout to further our understanding of the metabolism of this EFA in fish. Rainbow trout were fed a pulse of deuterated linolenic acid (D5-17,17,18,18,18-18:3n-3), and the tissue distribution of deuterated anabolites was ...

journal_title：Lipids

authors： Bell MV,Dick JR,Porter AE

更新日期：2003-09-01 00:00:00

abstract：:In the present study, the structural characteristics that impart antibacterial activity to C16 alkynoic fatty acids (aFA) were further investigated. The syntheses of hexadecynoic acids (HDA) containing triple bonds at C-3, C-6, C-8, C-9, C-10, and C-12 were carried out in four steps and with an overall yield of 34-78%...

journal_title：Lipids

authors： Sanabria-Ríos DJ,Morales-Guzmán C,Mooney J,Medina S,Pereles-De-León T,Rivera-Román A,Ocasio-Malavé C,Díaz D,Chorna N,Carballeira NM

更新日期：2020-03-01 00:00:00