Abstract:
:The molecular species of sphingoid bases were tagged with the fluorescent amino group reagent, 4-fluoro-7-nitrobenzofurazan (NBD-F). The NBD-sphingoid bases were analyzed by a highly selective and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) technique capable of reliable detection of several fmol of the derivatives. Lipid extracts from plant samples were derivatized with NBD-F, and all nine species of free sphingoid bases present in plant sphingolipids were separated and quantified for the first time; a complete baseline resolution was achieved for cis-8 and trans-8 isomers of sphingoid bases by reversed phase HPLC on a C18 column. The extraction and derivatization procedures and LC-MS/MS method can facilitate the progress of the studies for seeking the active components of sphingoid bases species in response to biological challenges.
journal_name
Lipidsjournal_title
Lipidsauthors
Ishikawa T,Imai H,Maki KYdoi
10.1007/s11745-013-3871-6subject
Has Abstractpub_date
2014-03-01 00:00:00pages
295-304issue
3eissn
0024-4201issn
1558-9307journal_volume
49pub_type
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