Abstract:
:Schizochytrium sp. is an algae-like microorganism utilized for commercial production of docosahexaenoic acid (DHA)-rich oil and dried microalgae for use as a source of DHA in foods, feeds, and nutritional supplements. Electron microscopic analysis of whole cells of Schizochytrium sp. employing sample preparation by high-pressure freeze substitution suggests the presence of secondary and tertiary semicrystalline structures of triacylglycerols within the oil bodies in Schizochytrium sp. A fine secondary structure consisting of alternating light- and dark-staining bands was observed inside the oil bodies. Dark bands were 29 +/- 1 A in width, and light bands were 22 +/- 1 A in width. The tertiary (three-dimensional) structure may be a multilayered ribbon-like structure which appears coiled and interlaced within the oil body. In freeze-fracture photomicrographs, Schizochytrium oil bodies exhibited fracture planes with terraces averaging 52 +/- 7 A in height which could correspond to the combined width of two halves of two light bands and one dark band observed in the high-pressure freeze substitution photomicrographs. The results suggest that triacylglycerols within Schizochytrium sp. oil bodies may be organized in a triple chain-length structure. High-pressure freeze substitution electron micrographs of two other highly unsaturated oil-producing species of microalgae, Thraustochytrium sp. and Isochrysis galbana, also revealed this fine structure, whereas microalgae containing a higher proportion of saturated oil did not. The results suggest that the staining pattern is not an artifact of preparation and that the triple chain-length conformation of triacylglycerols in Schizochytrium sp. oil bodies may be caused by the unique fatty acid composition of the triacylglycerols.
journal_name
Lipidsjournal_title
Lipidsauthors
Ashford A,Barclay WR,Weaver CA,Giddings TH,Zeller Sdoi
10.1007/s11745-000-0655-2subject
Has Abstractpub_date
2000-12-01 00:00:00pages
1377-86issue
12eissn
0024-4201issn
1558-9307journal_volume
35pub_type
杂志文章相关文献
LIPIDS文献大全abstract::The distribution of dolichol in rat liver was studied. Upon high-speed centrifugation, 9% of the total tissue dolichol was recovered in the supernatant. Dolichol was enclosed in vesicles and in lipidic particles which were isolated by gel filtration and density gradient centrifugation. The particles had a diameter of ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537050
更新日期:1992-01-01 00:00:00
abstract::This study addresses two questions: 1) what is the maximum amount of tocopherol that can be contained in egg phosphatidylcholine liposomes, and 2) what is the stability of these vesicles in the presence of serum proteins? These liposomes, made with a French pressure cell, can contain no more than 33 mol% of tocopherol...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534254
更新日期:1985-03-01 00:00:00
abstract::Human chylomicron triglyceride (TG) kinetics has been difficult to determine directly owing to technical limitations. This report describes a new method for studying chylomicron metabolism. Healthy volunteers (n = 10) sipped a drink providing 175 mg fat x kg(-1) h(-1) for 7.5 h to produce a steady-state chylomicronemi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0696-6
更新日期:2001-02-01 00:00:00
abstract::The effect of Rauscher Leukemia Virus (MuLV-R) infection on the lipid composition of mouse spleen from BALB/c mice was investigated. Drastic changes in the lipid composition of the spleen as a result of tumor growth induced by the virus could be demonstrated at 21 days after infection. The molar ratio of cholesterol t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533406
更新日期:1976-11-01 00:00:00
abstract::The levels of long-chain acyl-CoA in the livers of rats given diets containing various amounts of dietary oils were investigated. Increasing the amount of soybean oil in the diet from 5% to 25% (w/w) led to a 40% increase in long-chain acyl-CoA. With partially hydrogenated marine oil, a sigmoidal dose-response curve w...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534796
更新日期:1984-03-01 00:00:00
abstract::Saturated fatty acids are the most abundant fatty acids in the brain, however, there has been some debate regarding the ability of intact dietary saturated fatty acids to be incorporated into the brain. In the present study, we use compound specific isotope analysis to measure the natural abundance carbon isotopic sig...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12046
更新日期:2018-05-01 00:00:00
abstract::The skin surface lipid of the dog has been reported to contain a high proportion of diol diesters having a lower mobility on thin layer chromatography than diesters from other species in spite of containing similar fatty acid and diol components. In the present study, dog skin surface lipid was separated by preparativ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533265
更新日期:1977-10-01 00:00:00
abstract::The gut microbiota is proposed as a "metabolic organ" involved in energy utilization and is associated with obesity. Dietary intervention is one of the approaches for obesity management. Changes in dietary components have significant impacts on host metabolism and gut microbiota. In the present study, we examined the ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-017-4253-2
更新日期:2017-06-01 00:00:00
abstract::The beta-adrenergic system in cardiac muscle cells is influenced by the n-3 polyunsaturated fatty acid (PUFA) content in cell membranes. This study deals with the specific effect of docosahexaenoic acid (DHA) as compared to other n-3 PUFA, without modification of the arachidonic acid content. Increasing the DHA conten...
journal_title:Lipids
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::Subjects with echolucent carotid plaques have an increased risk of ischemic cerebrovascular events independent of degree of stenosis. Low plasma lipoprotein lipase (LPL) activity promotes a proatherogenic lipid profile, and delayed chylomicron clearance is a risk factor for atherosclerosis. This study was conducted to...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-008-3159-4
更新日期:2008-04-01 00:00:00
abstract::Tissue and blood fatty acid compositions are used as biological markers of fatty acid intakes to improve dietary assessments. These approaches are invasive and not well accepted, particularly in infants and young children. We developed a sensitive method for the analysis of fatty acids in cheek cell glycerophospholipi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-011-3576-7
更新日期:2011-10-01 00:00:00
abstract::Fatty acids of Ophiuroidea (brittle star) Ophiura sarsi have been investigated by gas-liquid chromatography (GLC). About 2-13% of four unidentified fatty acids were found in total fatty acids from a sample caught at a depth of 1,100 m. Structural analyses were undertaken after partial hydrogenation of their concentrat...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0854-x
更新日期:2001-12-01 00:00:00
abstract::In view of the findings that omega 3 fatty acids inhibit the synthesis of prostaglandins (PG) from arachidonic acid (20:4 omega 6) and that among immunologically active cells, the macrophage is a major producer of PG, we undertook a study of the effect of dietary alpha-linolenic acid (18:3 omega 3) on PG synthesis in ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535390
更新日期:1983-08-01 00:00:00
abstract::The amount and composition of lysophosphatidate present in different rat tissues have been estimated by an internal standard method in which a synthetic unnatural isomer (1-heptadecanoyl-rac-glycerol-3-phosphate) was added to the total lipid extracts, and the fatty acid composition of purified lysophosphatidate was de...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535172
更新日期:1989-04-01 00:00:00
abstract::Triacylglycerol (TAG) is the main storage lipid in plants. Acyl-CoA: diacylglycerol acyltransferase (DGAT1 and DGAT2) and phospholipid: diacylglycerol acyltransferase (PDAT) can catalyze TAG synthesis. It is unclear how these three independent genes are regulated in developing seeds, and particularly if they have spec...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-010-3385-4
更新日期:2010-02-01 00:00:00
abstract::The effects of increasing concentrations of eicosapentaenoic acid (20:5n-3; EPA) and oleic acid (18:1n-9; OA) on esterification to triacylglycerols (TG) and phospholipids (PL), and the relationship to formation and secretion of the very low density lipoproteins (VLDL) were compared in the isolated perfused rat liver. ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535940
更新日期:1993-05-01 00:00:00
abstract::Left ventricular (LV) remodeling after myocardial infarction constitutes the structural basis for ventricular dysfunction and heart failure. The characterization underlying the expression of lipoprotein receptors in cardiac dysfunction is scarcely explored. The aim of this study was to analyze the status of lipoprotei...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12014
更新日期:2018-02-01 00:00:00
abstract::The effectiveness of different fatty acids as inhibitors of fatty acyl delta 5 desaturation activity in human skin fibroblasts has been investigated. When incubated with 2.25 microM [14C] eicosatrienoate (20:3 omega 6) in otherwise lipid-free medium, these cells rapidly incorporate the radiolabeled fatty acid into cel...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534517
更新日期:1984-11-01 00:00:00
abstract::This study proposed lipid fingerprinting of human seminal plasma by mass spectrometry as an analytical method to differentiate biological conditions. For this purpose, we chose infertile men as a model to study specific conditions, namely: high and low seminal plasma lipid peroxidation levels (sub-study 1.1), high and...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3922-7
更新日期:2014-09-01 00:00:00
abstract::The alk-1-enyl group of 1-alk-1(1)-enyl-2-acyl-glycerophospholipids (plasmalogens) rapidly combines with mercuric chloride. At 0 C, there was a 1:1 stoichiometry for Hg binding to the reactive enol group of plasmalogens. Aldehydes were not released, indicating that the alkenyl ether bond was not cleaved. Hg binding to...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535374
更新日期:1982-09-01 00:00:00
abstract::Salmon farmers are currently using high-energy feeds containing up to 35% fat; the fish's capability of fully utilizing these high-energy feeds has received little attention. Carnitine is an essential component in the process of mitochondrial fatty acid oxidation and, with the cooperation of two carnitine palmitoyltra...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0289-4
更新日期:1998-09-01 00:00:00
abstract::In animals, non-sterol metabolites of the mevalonate pathway act independently from receptor-mediated cholesterol uptake in the multivalent feedback regulation of mevalonate biosynthesis. Studies leading to the isolation and characterization of plant-borne suppressors of mevalonate biosynthesis are reviewed. We propos...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534407
更新日期:1985-11-01 00:00:00
abstract::Conjugated linoleic acid (CLA) has been shown to cause a reduction in obesity in several species. CLA-induced body fat loss is enhanced when mice are fed coconut oil (CO) and involves increased lipolysis. The objective of this paper was to determine if the CLA-induced lipolysis in mice fed with different oil sources w...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-013-3835-x
更新日期:2013-11-01 00:00:00
abstract::1,2-Diacylglycerol, which has been recognized as one of the intracellular second messengers, was measured quantitatively in the lipid extract from rat hearts using the thin layer chromatography and flame ionization detection (TLC/FID) method. Cholesterol acetate was added to the tissue as an internal standard, and the...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535467
更新日期:1988-03-01 00:00:00
abstract::Four bacterial isolates, which produced polyunsaturated fatty acids (PUFA), were isolated from water samples of radioactive springs collected from Jáchymov spa. Jáchymov (Sankt Joachimsthal) is a city in northwestern Bohemia, where Marie and Pierre Curie isolated radium in 1898 from the mineral uraninite. To date, fou...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12136
更新日期:2019-02-01 00:00:00
abstract::The degradation of alpha-tocopherol and the formation of alpha-tocopherol and triacylglycerol oxidation products at high temperatures (150-250 degrees C) over a heating period (0-4 h) for a model system ranging between triolein and tripalmitin were modeled by use of an experimental design. The oxidation products of al...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0777-6
更新日期:2001-07-01 00:00:00
abstract::In a detailed sterol analysis of the marine diatom Phaeodactylum tricornutum, free sterols as well as esterified and glycosylated conjugates were found. When the alga was grown under standard conditions (i.e., at 13 degrees C under white light), 64% of total sterols were steryl glycosides. In all sterol classes, excep...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02522694
更新日期:1996-09-01 00:00:00
abstract::Red blood cell (RBC) fatty acid (FA) patterns have been shown to predict risk for cardiovascular and other chronic diseases. As part of a project analyzing RBC samples from the Women's Health Initiative Memory Study (WHIMS) we observed implausibly low levels of highly unsaturated fatty acids (HUFA) suggestive of degra...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-012-3693-y
更新日期:2012-09-01 00:00:00
abstract::Although migrating birds use stored triacylglycerol as their primary fuel for flight, they must retain sufficient stores of omega 6 and omega 3 fatty acids to sustain reproduction after the spring migration. Hepatic monoacylglycerol:acyl-coenzyme A acyltransferase (EC 2.3.1.22) (MGAT) activity is associated with physi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536701
更新日期:1994-11-01 00:00:00
abstract::Bioassay-guided fractionation of a collection of Moorea bouillonii from Papua New Guinea led to the isolation of a new alkyl amide, mooreamide A (1), along with the cytotoxic apratoxins A-C and E. The planar structure of 1 was elucidated by NMR spectroscopy and mass spectrometry analysis. Structural homology between m...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3949-9
更新日期:2014-11-01 00:00:00