Abstract:
:Human chylomicron triglyceride (TG) kinetics has been difficult to determine directly owing to technical limitations. This report describes a new method for studying chylomicron metabolism. Healthy volunteers (n = 10) sipped a drink providing 175 mg fat x kg(-1) h(-1) for 7.5 h to produce a steady-state chylomicronemia. A commercial 10% intravenous lipid emulsion was labeled with [3H]triolein, purified by high-performance liquid chromatography, and sterilized. A trace amount of labeled emulsion was injected intravenously 30 min before (i.e., in the fasting state) and 5, 6, and 7 h after sipping began (i.e., triplicate determinations in the fed state). Chylomicron half-lives were calculated from the monoexponential decay curves, and apparent distribution volumes were estimated by back-extrapolation to time zero. Plasma and estimated chylomicron TG concentrations increased from 89+/-13 and 0.8+/-0.3 to 263+/-43 and 91+/-39 mg/dL (mean +/- SEM), respectively, with feeding. Tracer-determined chylomicron TG half-lives were 5.34+/-0.58 and 6.51+/-0.58 min during the fasting and fed states, respectively (P < 0.01). The apparent distribution volume during the fasting state was 24% greater than plasma volume (4515+/-308 vs. 3630+/-78 mL, P < 0.02), consistent with significant margination of lipid emulsion particles to endothelial binding sites. Margination was reduced during the fed state, suggesting that native chylomicrons competed with lipid emulsion particles for endothelial lipoprotein lipase. The results indicate that a radiolabeled commercial lipid emulsion is metabolized in a fashion similar to native chylomicron TG, and thus can be used to study chylomicron TG kinetics.
journal_name
Lipidsjournal_title
Lipidsauthors
Park Y,Damron BD,Miles JM,Harris WSdoi
10.1007/s11745-001-0696-6subject
Has Abstractpub_date
2001-02-01 00:00:00pages
115-20issue
2eissn
0024-4201issn
1558-9307journal_volume
36pub_type
杂志文章相关文献
LIPIDS文献大全abstract::The effect of 4,4'-diethylamino-ethoxyhexestrol (DH) on acid esterase in rat liver was studied in vivo and in vitro. The acid esterase activity in the livers of rats treated with 0.125% DH for 1 week was found to decrease more than 60% as compared with that in untreated rats. The addition of DH to the incubation mediu...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533044
更新日期:1976-10-01 00:00:00
abstract::In humans and animals, intestinal flora is indispensable for bile acid transformation. The goal of our study was to establish gnotobiotic mice with intestinal bacteria of human origin in order to examine the role of intestinal bacteria in the transformation of bile acids in vivo using the technique of gnotobiology. Ei...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-5038-1
更新日期:2006-09-01 00:00:00
abstract::Flaxseed oil is an alpha linolenic acid source important in the growth and body development stage; furthermore, this acid acts on adipose tissue and bone health. The aim of this study was to evaluate body composition, fatty acid composition, hormone profile, retroperitoneal adipocyte area and femur structure of pups a...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-016-4165-6
更新日期:2016-08-01 00:00:00
abstract::The aim of the present work was to study the effect of season on phospholipids and triacylglycerols (TAG) of mantle and tentacles of female and male wild Sepia officinalis. The identified phospholipids were phosphatidylethanolamine (PtdEtn), phosphatidylcholine (PtdCho), phosphatidylserine (PtdSer), and phosphatidylin...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12137
更新日期:2019-02-01 00:00:00
abstract::Synthetic activators of peroxisome proliferator-activated receptors (PPAR)-alpha and -gamma are capable of reducing macrophage foam cell cholesterol accumulation through the activation of genes involved in cholesterol homeostasis. Since conjugated linoleic acids (CLA) were also demonstrated to activate PPARalpha and P...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-008-3226-x
更新日期:2008-10-01 00:00:00
abstract::Membrane phospholipid (PL) composition has been shown to affect cellular function by altering membrane physical structure. The sarcolemma plasma membrane (SLpm) is integral to skeletal muscle function and health. Previous studies assessing SLpm PL composition have demonstrated contamination from transverse (t)-tubule,...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-013-3770-x
更新日期:2013-04-01 00:00:00
abstract::The particular interest in supplementing human foods with n-3 fatty acids has arisen from the findings that this series of polyunsaturated fatty acids (PUFA) have an impact on neuronal functions. Indeed vertebrates, including humans, preferentially use docosahexaenoic acid (DHA, 22:6n-3) over other long-chain n-3 PUFA...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-012-3686-x
更新日期:2012-08-01 00:00:00
abstract::Fatty acids are involved in the regulation of inflammatory responses in obesity. However, despite being the largest dietary fatty-acid class, effects of 18-carbon fatty acids with different degrees of saturation on inflammatory, metabolic, and thermogenic markers have not been well studied. Therefore, the objective of...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12101
更新日期:2018-09-01 00:00:00
abstract::Although migrating birds use stored triacylglycerol as their primary fuel for flight, they must retain sufficient stores of omega 6 and omega 3 fatty acids to sustain reproduction after the spring migration. Hepatic monoacylglycerol:acyl-coenzyme A acyltransferase (EC 2.3.1.22) (MGAT) activity is associated with physi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536701
更新日期:1994-11-01 00:00:00
abstract::Fatty acid synthetase purified (20 times) from lactating bovine mammary tissue had an approximate mot wt of 485,000. The enzyme had a high content of acidic and hydrophobic amino acid residues; 62±4 sulhydryl groups and one 4' phosphopantetheine residue/mole of enzyme. The enzyme was relatively stable when stored (3 m...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532485
更新日期:1975-04-01 00:00:00
abstract::The effects of 2-hexadecynoic acid on the growth and lipid metabolism of cultured 7288 (HTC) cells have been evaluated. Growth was inhibited by the acetylenic acid: the LD50 was 35-85 microM as determined by two methods at low and high cell densities. Reduced growth did not result from damaged plasma membranes as dete...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534955
更新日期:1981-05-01 00:00:00
abstract::The cholesterol-suppressive action of the tocotrienol-rich-fraction (TRF) of palm oil may be due to the effect of its constituent tocotrienols on beta-hydroxy-beta-methylglutaryl coenzyme A (HMG-CoA) reductase activity. The tocotrienols, modulate HMG-CoA reductase activity via a post-transcriptional mechanism. As a co...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-5119-1
更新日期:2006-05-01 00:00:00
abstract::The activity of pyruvate dehydrogenase from freshly isolated mitochondria was shown to be dependent upon the nutritional and metabolic state of the animal prior to sacrifice, such that mitochondria from the livers of 48 hr starved, diabetic, or high fat fed rats had lower enzyme activity than normal, chow fed rats. Th...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532317
更新日期:1975-12-01 00:00:00
abstract::Commercial preparations of Candida rugosa lipase (CRL) are mixtures of lipase isoforms used for the hydrolysis and synthesis of various esters. The presence of variable isoforms and the amount of lipolytic protein in the crude lipase preparations lead to a lack of reproducibility of biocatalytic reactions. Purificatio...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-004-1258-7
更新日期:2004-06-01 00:00:00
abstract::The production of cardiac arrhythmias and the elevation of lysophosphatidylcholine level in the ischemic myocardium have been well-documented in a number of studies. However, the relationship between the production arrhythmias and the elevation of tissue lysophosphatidylcholine level was not reported. In this study, t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535301
更新日期:1988-01-01 00:00:00
abstract::The purpose of this study was to assess changes in the degree of fatty acid unsaturation in rat liver after partial hepatectomy. This is the first study in which liver fatty acid unsaturation has been analyzed over a long period of regeneration until day 28 after operation. The relationship between changes in unsatura...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-000-543-9
更新日期:2000-04-01 00:00:00
abstract::Four bacterial isolates, which produced polyunsaturated fatty acids (PUFA), were isolated from water samples of radioactive springs collected from Jáchymov spa. Jáchymov (Sankt Joachimsthal) is a city in northwestern Bohemia, where Marie and Pierre Curie isolated radium in 1898 from the mineral uraninite. To date, fou...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12136
更新日期:2019-02-01 00:00:00
abstract::Trans FA (TFA) have at least one trans double bond and comprise several isomers and types, including many of the CLA (e.g., c9,t11-18:2 CLA). Some TFA may have adverse effects (e.g., cardiovascular disease), whereas some are thought to have beneficial effects (e.g., anticarcinogenicity). The presence of TFA in human t...
journal_title:Lipids
pub_type: 杂志文章,随机对照试验
doi:10.1007/s11745-006-5082-x
更新日期:2006-02-01 00:00:00
abstract::This review covers the work of our laboratory on the antineoplastic activity of some ether lipids and derivatives that are related to platelet-activating factor (PAF). Various 1-O-alkyl lysophospholipid derivatives (ALP) show therapeutic activity in mouse transplant tumor models and in metastatic 3-Lewis lung carcinom...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/BF02535567
更新日期:1987-11-01 00:00:00
abstract::The levels of long-chain acyl-CoA in the livers of rats given diets containing various amounts of dietary oils were investigated. Increasing the amount of soybean oil in the diet from 5% to 25% (w/w) led to a 40% increase in long-chain acyl-CoA. With partially hydrogenated marine oil, a sigmoidal dose-response curve w...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534796
更新日期:1984-03-01 00:00:00
abstract::Fatty acids of Ophiuroidea (brittle star) Ophiura sarsi have been investigated by gas-liquid chromatography (GLC). About 2-13% of four unidentified fatty acids were found in total fatty acids from a sample caught at a depth of 1,100 m. Structural analyses were undertaken after partial hydrogenation of their concentrat...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0854-x
更新日期:2001-12-01 00:00:00
abstract::Peroxidation of intact human erythrocytes by t-butylhydroperoxide (tBHP)) was studied. By incubation of the erythrocytes with 1 mM tBHP, reduced glutathione (GSH) was exhausted within 1 min, and tocopherols (Toc) and phospholipids (PL) decreased to nearly their lowest levels (in this study) within 5 min. The rate of d...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0668-x
更新日期:2001-01-01 00:00:00
abstract::Corn oil, peanut oil and randomized peanut oil exhibit different atherogenic potentials; peanut oil being more atherogenic than the other oils. This study was conducted to ascertain if the atherogenicity of these oils was related to their rates of lipolysis. Using both pancreatic lipase and milk lipoprotein lipase (LP...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534645
更新日期:1983-11-01 00:00:00
abstract::The relationship between LCAT mediated HDL modification and the redistribution of lipoprotein-unassociated apoA-IV to HDL was investigated in vitro. Immunoaffinity-isolated rat lipoprotein-unassociated apoA-IV was added to apoB-, apoE-, apoA-IV depleted, [3H]cholesterol labelled rat plasma and incubated at 37 degrees ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544075
更新日期:1989-12-01 00:00:00
abstract::The fatty acid (FA) profile of oysters generally reflects the dietary FA composition. Moreover, incorporation of FA into tissues is modulated by various metabolic factors, and final composition will depend upon the dietary sources, cumulative intake, and oysters' development stage. Thus, the aim of this study was to a...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12177
更新日期:2019-09-01 00:00:00
abstract::The importance of n-6 and n-3 polyunsaturated fatty acids (PUFA) in neonatal development, particularly with respect to the developing brain and retina, is well known. This review combines recent information from basic science and clinical studies to highlight recent advances in knowledge on PUFA metabolism and areas w...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-999-0348-x
更新日期:1999-02-01 00:00:00
abstract::The conversion of [14C]-labeled compounds such as acetate, glucose, pyruvate and palmitate into CO2, glyceride-glycerol, glyceride fatty acids and total lipids was monitored in the average and matching adipocyte (with respect to size) preparations from young (6-9 wk) and old (age 56-60 wk) male Sprague-Dawley rats. Th...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536404
更新日期:1986-07-01 00:00:00
abstract::This report deals with the fluorometric determination of fatty alcohols generated by the reduction of the ester linkage of lipids with NaBH4, and with the limitations of the reduction method for assaying oxidized lipids. Optimum conditions for the fluorometric analysis of primary and secondary alcohols using 1-anthroy...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537169
更新日期:1990-09-01 00:00:00
abstract::Chlorpromazine (CPZ), a major tranquilizer, was found to be a potent inhibitor of lecithin:cholesterol acyltransferase (LCAT, EC 2.3.1.43) in the plasma of normal man, rat, rabbit and dog in vitro. The inhibitory effect of CPZ reached 35-50% at 0.5 mM depending on species; dog plasma LCAT appeared to be somewhat more ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535035
更新日期:1981-11-01 00:00:00
abstract::In order to determine the minimal amount of dietary 9-trans, 12-trans-linoleate which can decrease endoperoxide metabolites synthesized and their precursor in rat platelets, graded amounts (0, 0.1, 0.5, 1.0, 2.5%) of the trans-linoleate were fed to rats with a constant amount of all-cis-linoleate (2.5%) for 12 weeks. ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534946
更新日期:1982-04-01 00:00:00