Abstract:
:A new method of isoelectric focusing (IEF) and pH gradient electrophoresis, using thin layers of agarose gel beads, was devised to investigate chylomicrons and very low density lipoproteins (VLDL). pH gradient stability and cathodal gradient drift were similar to those of polyacrylamide gel IEF, and linearity of gradients was maintained for 23 hr. Chylomicrons and VLDL were detectable without staining. Chylomicrons from human serum and from rat lymph migrated in this system. Rat lymph chylomicrons, obtained by ultracentrifugation, migrated in several discrete bands, and this heterogeneity or rat chylomicrons was confirmed by electron microscopic demonstration of chylomicrons in each band. This new technique has permitted the first measurement of isoelectric points of some lipoproteins in the ultracentrifuged fraction of human serum chylomicrons and the first separation of multiple discrete fractions of ultracentrifuged lymph chylomicrons.
journal_name
Lipidsjournal_title
Lipidsauthors
Vost A,Pocock DM,Pleet Sdoi
10.1007/BF02534130subject
Has Abstractpub_date
1979-10-01 00:00:00pages
864-71issue
10eissn
0024-4201issn
1558-9307journal_volume
14pub_type
杂志文章相关文献
LIPIDS文献大全abstract::An improved headspace capillary gas chromatographic (GC) method was developed to measure the oxidative susceptibility of human red blood cell (RBC) membranes. This method analyzed volatile peroxidation products of both n-6 (hexanal and pentane) and n-3 (propanal) polyunsaturated fatty acids. Oxidative susceptibility t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536076
更新日期:1991-06-01 00:00:00
abstract::Steroids bearing ketone functionality at carbon-7 are found commonly in nature, and the most prevalent of these are the 7-keto-delta5-sterols. These substances have diverse biological properties and are present in biological samples and food products. For the purpose of studying this class of oxysterols, many chemical...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-004-1299-y
更新日期:2004-08-01 00:00:00
abstract::Oxidized LDL (oxLDL) may contribute to the accumulation of apoptotic cells in atherosclerotic plaques. Although it is well established in monophasic chemical systems that the highly unsaturated EPA and DHA will oxidize more readily than FA that contain fewer double bonds, our previous studies showed that enrichment of...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0962-7
更新日期:2002-08-01 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) often occurs concurrently with high blood cholesterol or dyslipidemia. Although T2DM has been hypothesized to impair bone microstructure, several investigations showed that, when compared to age-matched healthy individuals, T2DM patients had normal or relatively high bone mineral densit...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3950-3
更新日期:2014-10-01 00:00:00
abstract::CLA inhibits mammary cancer and reduces body fat accumulation in rodents. It is not known whether uncoupling proteins (UCP), which are modulators of energy balance and metabolism, play a role in these actions of CLA. To determine the effects of dietary CLA on the expression of UCP in various tissues, 5-wk-old Sprague-...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0971-6
更新日期:2002-09-01 00:00:00
abstract::A simple method is described for the preparation of [6,7,7(-2)H3] sterols and steroids. The synthesis starts with a Δ(5)-sterol or steroid and involves preparation of the 6-oxo-3α,5α-cyclosteroid, base exchange in the presence of deuterium oxide to introduce two deuteriums at the C-7 position and sodium borodeuteride ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534596
更新日期:1982-12-01 00:00:00
abstract::A rat liver cytosolic cholesteryl ester hydrolase (CEH) was purified 12,600-fold by ammonium sulfate precipitation, cation exchange chromatography and gel permeation high-performance liquid chromatography, with an overall yield of 20%. Its properties are compared to those of pancreatic CEH, with which it has sometimes...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536160
更新日期:1991-10-01 00:00:00
abstract::The catfish, Ictalurus punctatus, is an important model for studying the biochemical mechanisms of taste at the peripheral level. The type, amount and metabolic activity of the lipids within this tissue play important roles in taste transduction by forming the matrix in which the receptors for taste stimuli are imbedd...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535745
更新日期:1990-04-01 00:00:00
abstract::The effect of dietary n-3 FA deficiency on bone tissue FA composition was evaluated in growing rats. Two mixtures combining hydrogenated coconut oil with safflower oil served as the n-3-deficient dietary treatments and provided two levels of linoleic acid (LA). The n-3 treatments were formulated with added alpha-linol...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-003-1115-8
更新日期:2003-06-01 00:00:00
abstract::This study addresses two questions: 1) what is the maximum amount of tocopherol that can be contained in egg phosphatidylcholine liposomes, and 2) what is the stability of these vesicles in the presence of serum proteins? These liposomes, made with a French pressure cell, can contain no more than 33 mol% of tocopherol...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534254
更新日期:1985-03-01 00:00:00
abstract::This work reports the effect of the apolipoproteins A-I and A-II (apoA-I and apoA-II) on the release of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-8, and IL-1 receptor antagonist (IL-1Ra) and on the oxidative burst of human neutrophils. By themselves, apoA-I and apoA-II do not affect the basal liber...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0981-4
更新日期:2002-09-01 00:00:00
abstract::De novo synthesis and mitochondrial elongation of fatty acids have been demonstrated in subcellular fractions from hog and human aorta. Microsomal fatty acid elongation has been shown in hog aorta. The activity catalyzing the formation of fatty acids from acetyl and malonyl CoA was associated with a high molecular wei...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533461
更新日期:1979-05-01 00:00:00
abstract::There is convincing evidence that normal cultured human keratinocytes possess a 15-lipoxygenase activity which, however, does not appear to manifest itself without cell membrane damage. When "activated", this enzyme transforms arachidonic acid into 15-hydroxyeicosatetraenoic acid (15-HETE), and linoleic acid into 13-h...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536012
更新日期:1990-10-01 00:00:00
abstract::Palm oil is one of the most important edible oils in the world. Its composition (rich in palmitate and oleate) make it suitable for general food uses but its utility could be increased if its fatty acid quality could be varied. In this study, we have modified a palm olein fraction by transesterification with the n-3 p...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-009-3304-8
更新日期:2009-07-01 00:00:00
abstract::Lifibrol, a new hypocholesterolemic agent with activity in humans, was examined in normal rats for its short-term and long-term effects on lipid homeostasis. Cholesterol (Chol) synthesis inhibition by lifibrol was demonstrated in vitro in liver minces from normal rats by following [1-14C]acetate ([14C]Ac) and DL-[2-14...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537074
更新日期:1993-12-01 00:00:00
abstract::Commercial preparations of Candida rugosa lipase (CRL) are mixtures of lipase isoforms used for the hydrolysis and synthesis of various esters. The presence of variable isoforms and the amount of lipolytic protein in the crude lipase preparations lead to a lack of reproducibility of biocatalytic reactions. Purificatio...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-004-1258-7
更新日期:2004-06-01 00:00:00
abstract::Omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA) are essential components of the diet of all vertebrates. The major dietary source of n-3 LC-PUFA for humans has been fish and seafood but, paradoxically, farmed fish are also reliant on marine fisheries for fish meal and fish oil (FO), traditionally major ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-016-4191-4
更新日期:2016-10-01 00:00:00
abstract::To better understand how docosahexaenoic acid (DHA) improves the effects of doxorubicin (DOX), we examined DHA ± DOX on changes in whole cell and lipid raft phospholipids (PL) of MDA-MB-231 and MCF-7 breast cancer cells. We sought to confirm whether the relative changes in PL DHA content of MDA-MB-231 cells could be e...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12252
更新日期:2020-09-01 00:00:00
abstract::Postprandial chylomicrons are potent ultimate acceptors of cell membrane cholesterol and are believed to accelerate reverse cholesterol transport (RCT). We compared the effects of meals rich in polyunsaturated fat (PUFA) and either high (605 mg) or low (151 mg) in cholesterol and a meal rich in dairy fat (DF) in the f...
journal_title:Lipids
pub_type: 杂志文章,随机对照试验
doi:10.1007/s11745-007-3101-1
更新日期:2007-10-01 00:00:00
abstract::Dietary fatty acids have different effects on fat deposition in pigs. To clarify the underlying mechanisms of this difference, we compared the metabolism of palmitic (PAM, saturated), oleic (OLA, monounsaturated) and linoleic acid (LNA, polyunsaturated) in porcine adipocytes treated with 100 μM PAM, OLA or LNA. We obs...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-017-4302-x
更新日期:2017-11-01 00:00:00
abstract::The 4-monomethylsterol and 4,4-dimethylsterol fractions were separated from the unsaponifiables of 20 vegetable oils by preparative thin layer chromatography, and their compositions were determined by gas liquid chromatography. Tentative identification of the individual components of these fractions was carried out by...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532729
更新日期:1975-10-01 00:00:00
abstract::The positional distribution of 22ratio6 and 22ratio5 in aquatic animal triglycerides can be predicted by simple proportionality equations of the typey=kx. The mole % 22ratio6 at the 1, 2, or 3 position (y) is obtained by multiplying the proportionality constant for that position (k (1),k (2), ork (3)) times the mole %...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02531280
更新日期:1968-09-01 00:00:00
abstract::A fast and efficient shotgun lipidomics strategy was applied to analyze phospholipids (PL) in the oyster Crassostrea plicatula, including 29 species of phosphatidylcholine (PtdCho), 23 species of phosphatidylethanolamine (PtdEtn), 11 species of phosphatidylserine (PtdSer), 6 species of phosphatidylinositol (PtdIns), a...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-017-4305-7
更新日期:2017-12-01 00:00:00
abstract::A sucrose-rich diet (SRD) induces insulin resistance and dyslipidemia with impaired hepatic glucose production and gluconeogenesis, accompanied by altered post-receptor insulin signaling steps. The aim of this study was to examine the effectiveness of fish oil (FO) to reverse or improve the impaired hepatic glucose me...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-011-3623-4
更新日期:2012-02-01 00:00:00
abstract::Ceramide 2-aminoethylphosphonate (CAEP), a sphingophosphonolipid containing a carbon-phosphorus bond, is frequently found in marine organisms and has a unique triene type of sphingoid base in its structure. CAEP has not been evaluated as a food ingredient, although it is generally contained in Mollusca organisms such ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-017-4239-0
更新日期:2017-04-01 00:00:00
abstract::Usually, the chemical structures of cerebrosides in sea creatures are more complicated than those from terrestrial plants and animals. Very little is known about the method for high-throughput molecular profiling of cerebrosides in sea cucumbers. In this study, cerebrosides from four species of edible sea cucumbers, s...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-015-4039-3
更新日期:2015-07-01 00:00:00
abstract::In the present paper, problems in connection with assay of the activity of magnesium-dependent rat liver phosphatidate phosphohydrolase (PAP) are discussed. PAP activity is usually measured by following the production of diacylglycerol or inorganic phosphate from the substrate phosphatidate. These two methods may give...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02522468
更新日期:1996-10-01 00:00:00
abstract::We have previously reported that the relative content of 18:3 omega 3 in adipose triglyceride (TG) of women was reduced following major weight loss while on a very low calorie diet (VLCD). In an attempt to prevent this loss of 18:3 omega 3 reserves, we have tested two VLCD supplemented with varying amounts of 18:3 ome...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537119
更新日期:1993-11-01 00:00:00
abstract::The synthesis of alkyl chain-substituted thiazanones from oxo fatty esters and a long chain aldehyde is reported. Four oxo compounds-methyl 10-oxoundecanoate, methyl 9-oxooctadecanoate, methyl 9,10-dioxooctadecanoate and octadecanal-were allowed to react with β-mercaptopropionic acid in the presence of ammonium carbon...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537284
更新日期:1987-08-01 00:00:00
abstract::A one-carbon degradation of long-chain fatty acids, which was found to occur in the brains of rats in vivo, has been investigated in a brain microsomal fraction in vitro. Decarboxylation of the alpha-hydroxy acid, a possible intermediate product between the substrate and the next shorter acid, in the presence of brain...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02531201
更新日期:1968-05-01 00:00:00