Abstract:
:Ceramide 2-aminoethylphosphonate (CAEP), a sphingophosphonolipid containing a carbon-phosphorus bond, is frequently found in marine organisms and has a unique triene type of sphingoid base in its structure. CAEP has not been evaluated as a food ingredient, although it is generally contained in Mollusca organisms such as squids and shellfish, which are consumed worldwide. In this study, we aimed to elucidate the effects of CAEP as a food component by evaluating the digestion of CAEP extracted from the skin of the jumbo flying squid Dosidicus gigas. Our results revealed that dietary CAEP was digested to free sphingoid bases via ceramides by the mouse small intestinal mucosa. At pH 7.2, CAEP was hydrolyzed more rapidly than the major mammalian sphingolipid sphingomyelin; however, the hydrolysis of CAEP was similar to that of sphingomyelin at pH 9.0. Thus, the digestion of CAEP may be catalyzed by alkaline spingomyelinase and other enzymes. Our findings provide important insights into the digestion of the dietary sphingophosphonolipid CAEP in marine foods.
journal_name
Lipidsjournal_title
Lipidsauthors
Tomonaga N,Manabe Y,Sugawara Tdoi
10.1007/s11745-017-4239-0subject
Has Abstractpub_date
2017-04-01 00:00:00pages
353-362issue
4eissn
0024-4201issn
1558-9307pii
10.1007/s11745-017-4239-0journal_volume
52pub_type
杂志文章相关文献
LIPIDS文献大全abstract::The effectiveness of different fatty acids as inhibitors of fatty acyl delta 5 desaturation activity in human skin fibroblasts has been investigated. When incubated with 2.25 microM [14C] eicosatrienoate (20:3 omega 6) in otherwise lipid-free medium, these cells rapidly incorporate the radiolabeled fatty acid into cel...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534517
更新日期:1984-11-01 00:00:00
abstract::A new triterpene alcohol was isolated fron shea butter and its structure was shown to be 24-methylenelanost-9(11)-en-3beta-ol. Gas chromatographic correlations between this triterpene alcohol and other related compounds are discussed. ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532428
更新日期:1975-08-01 00:00:00
abstract::A new spectrophotometric assay for determining the activity of acylglycerol hydrolases (lipases, E.C. 3.1.1.3) was developed and optimized for yeast lipase (Candida cylindracea). Studies with porcine pancreatic lipase were also conducted and the influence of various detergents and divalent cations on the assay was eva...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535132
更新日期:1989-06-01 00:00:00
abstract::Recently, lipases have been purified from mammalian, bacterial, fungal and plant sources by different methodologies. Purified lipases subsequently have been characterized for molecular size, metal binding capabilities, glycoside and phosphorus contents, and substrate specificities. Primary structures of several lipase...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/BF02535273
更新日期:1988-12-01 00:00:00
abstract::Bile acids solubilize proteins from liver plasma membrane both in vivo and in vitro. The ability to solubilize the proteins is dependent on the species of bile acid. In this paper, the effect of phospholipid on the solubilization of a membrane-bound enzyme by bile acids was investigated in vitro. Taurocholate (TC) and...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534873
更新日期:1987-01-01 00:00:00
abstract::Some 1,2- and 1,3-diacyl glycerols (with acyl groups as stearyl, oleyl, linoleyl, or stearolyl) were synthesized by conventional methods. The diacyl glycerols were esterified with 6-bromo-hexanoic acid to give the corresponding bromo-triacylglycerols (of the type AAB and ABA containing a bromo group at the distal part...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0769-6
更新日期:2001-06-01 00:00:00
abstract::The effects of increasing concentrations of eicosapentaenoic acid (20:5n-3; EPA) and oleic acid (18:1n-9; OA) on esterification to triacylglycerols (TG) and phospholipids (PL), and the relationship to formation and secretion of the very low density lipoproteins (VLDL) were compared in the isolated perfused rat liver. ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535940
更新日期:1993-05-01 00:00:00
abstract::In the presence of iodide and hydrogen peroxide, lactoperoxidase catalyzed the conversion of arachidonic acid into several iodinated products; the major one was previously identified as an iodo-delta-lactone. Two minor and less polar products have now been characterized as 15-iodo-14-hydroxyeicosatrienoic acid, omega-...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535024
更新日期:1981-04-01 00:00:00
abstract::Biosynthesis of peripheral nerve cholesterol was investigated by the in vivo and in vitro incorporation of [1-14C]-acetate into sciatic endoneurium of normal rats during development, degeneration and regeneration. Labeled sterols were rapidly formed (less than 10 min) within the endoneurial portion of sciatic nerve af...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536205
更新日期:1988-09-01 00:00:00
abstract::For three weeks, male Sprague-Dawley rats at either four weeks (young) or eight months (adult) of age were pair-fed one of the purified diets free of or containing either 0.2% of oxidized cholesterol mixture (cholesterol oxidation products) or 0.2% of cholesterol. Although the food intake was similar, dietary oxidized...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536298
更新日期:1995-05-01 00:00:00
abstract::The major sterols of the seeds ofBenincasa cerifera, Cucumis sativus, Cucurbita maxima, C. pepo andTrichosanthes japonica and of the mature plant tissues (leaves and stems) ofCitrullus battich, Cucumis sativus andGynostemma pentaphyllum of the family Cucurbitaceae were 24-ethyl-Δ(7)-sterols which were accompanied by s...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534301
更新日期:1986-01-01 00:00:00
abstract::The growth inhibitory effects of 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine (ET-18-OCH3) and various liposome compositions of ET-18-OCH3 were compared in a standardized growth inhibition assay utilizing a diverse tumor cell line panel including cell lines expressing multidrug resistance. ET-18-OCH3 and ELL-1...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-997-0135-8
更新日期:1997-10-01 00:00:00
abstract::Vegetable oils are one of the main agricultural commodities. Demand has been increasing steadily over the last five decades and, with finite land available, it is vital that we increase productivity. My laboratory has focused on the regulation of plant lipid metabolism and, as part of this work, we identified diacylgl...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12267
更新日期:2020-09-01 00:00:00
abstract::A new method of isoelectric focusing (IEF) and pH gradient electrophoresis, using thin layers of agarose gel beads, was devised to investigate chylomicrons and very low density lipoproteins (VLDL). pH gradient stability and cathodal gradient drift were similar to those of polyacrylamide gel IEF, and linearity of gradi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534130
更新日期:1979-10-01 00:00:00
abstract::Carbohydrate response element binding protein (ChREBP) regulates insulin-independent de novo lipogenesis. Recently, a novel ChREBPβ isoform was identified. The purpose of the current study was to define the effect of dietary carbohydrates (CHO) and obesity on the transcriptional activity of ChREBP isoforms and their r...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-015-4090-0
更新日期:2016-01-01 00:00:00
abstract::The conversion of labeled palmitic, linoleic, arachidonic and docosahexaenoic acids to their respective acyl CoA's was studied in homogenates and microsomes of rat tissues. The highest activity, both in homogenates and microsomes, was seen in liver and heart. There was moderate activity in retina, brain, lung, kidney ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534740
更新日期:1984-12-01 00:00:00
abstract::Both diacyl GPC (glycerylphosphorylcholine) and diacyl GPE (glycerylphosphorylethanolamine) are synthesized in human placental tissue from their respective monoacyl precursors. The origin of the monacyl phosphatides is apparently not the result of placental phosphatide acyl-hydrolase activity. The most likely source i...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02531854
更新日期:1967-09-01 00:00:00
abstract::We have developed cultures of smooth muscle cells (SMC) isolated from arterial hypercholesterolemic chicks (cholesterol-SMC). These cultures are suitable for the study at the molecular level of the changes in arterial SMC induced by a cholesterol diet. By using a strong dose of cholesterol (5%) for 10 d, we obtained v...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0194-x
更新日期:1998-02-01 00:00:00
abstract::We hypothesized that tumor-bearing (TB) nude mice, because they are reported to have no detectable, circulating tumor necrosis factor (TNF)/cachectin, would regulate their plasma free fatty acid (FFA) levels normally when fasted and refed. We compared levels of individual plasma FFA in response to fasting (24 hr) and ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537055
更新日期:1992-01-01 00:00:00
abstract::The amount and composition of lysophosphatidate present in different rat tissues have been estimated by an internal standard method in which a synthetic unnatural isomer (1-heptadecanoyl-rac-glycerol-3-phosphate) was added to the total lipid extracts, and the fatty acid composition of purified lysophosphatidate was de...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535172
更新日期:1989-04-01 00:00:00
abstract::Total lipids from whole pig adrenal glands as well as from their mitochondria, microsomes, liposomes, and cell sap were extracted and fractionated first into neutral lipids and phospholipids. The highest percentage of neutral lipids was found in the cell sap, and the lowest in the microsomal fraction. Neutral lipids w...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532765
更新日期:1975-11-01 00:00:00
abstract::This study evaluated the effects of conjugated linoleic acids (CLA) on tissue fatty acid composition and ex vivo prostaglandin E2 (PGE2) production in rats given diets varying in n-6 and n-3 fatty acids. Four groups of rats were given a basal semipurified diet (AIN-93G) containing 70 g/kg of added fat for 42 d. The fa...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0223-9
更新日期:1998-04-01 00:00:00
abstract::An unidentified bacterial strain, SCRC-21406, isolated from the intestine of a marine fish, Glossanodon semifasciatus, produced docosahexaenoic acid at 23% (mol/mol) [= 28% (w/w)] of total fatty acids in a medium containing 0.5% (wt/vol) peptone and 0.1% (wt/vol) yeast extract at 12 degrees C under atmospheric pressur...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-997-0127-8
更新日期:1997-09-01 00:00:00
abstract::The relationship between LCAT mediated HDL modification and the redistribution of lipoprotein-unassociated apoA-IV to HDL was investigated in vitro. Immunoaffinity-isolated rat lipoprotein-unassociated apoA-IV was added to apoB-, apoE-, apoA-IV depleted, [3H]cholesterol labelled rat plasma and incubated at 37 degrees ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544075
更新日期:1989-12-01 00:00:00
abstract::Many reports have shown the beneficial effects of consumption of pine seeds and pine seed oil. However, few studies have examined the biological effect of pinolenic acid (PNA; 5,9,12-18:3), the main fatty acid in pine seed oil. In this study, using murine macrophage RAW264.7 cells as a model, we examined the effect of...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-008-3276-0
更新日期:2009-03-01 00:00:00
abstract::Primary cultured hepatocytes from normal mice and mice with Sarcoma 180 were characterized. The viability of freshly isolated hepatocytes from both sources was over 90% and the cells had a relatively stable population of DNA for a minimum of three days. After incubation with (3H)leucine, the syntheses and secretions o...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534229
更新日期:1985-07-01 00:00:00
abstract::Oxidized LDL (oxLDL) may contribute to the accumulation of apoptotic cells in atherosclerotic plaques. Although it is well established in monophasic chemical systems that the highly unsaturated EPA and DHA will oxidize more readily than FA that contain fewer double bonds, our previous studies showed that enrichment of...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0962-7
更新日期:2002-08-01 00:00:00
abstract::Intact washed human platelets aggregated in response to paf-acether (paf) and did not metabolize [3H]paf at concentrations up to 10 nM. However, when platelets were lysed by exposure to pH 9.5, resulting in 37.5 +/- 2.5% (mean +/- SD, n = 3) lactic dehydrogenase (LDH) release, 20.5 +/- 5.7% of the radioactivity was de...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536639
更新日期:1993-03-01 00:00:00
abstract::A quantitative method for the simultaneous determination of alk-l-enyl- and alkyl-glyceryl ethers is described. Complete hydrogenolysis of carboxylate and phosphate esters of neutral lipids and phospholipids was achieved with lithium aluminum hydride. The hydrogenolysis products of the glyceryl ether containing lipids...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02531729
更新日期:1968-03-01 00:00:00
abstract::This study investigated the influence of PUFA on the properties of mouse skin. Mice (3 wk old) were given free access to oils high in linoleic acid, CLA, or DHA for 4 wk. At the end of the experiment, their skins were compared by both biochemical and histological methods. No significant differences in lipid and collag...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-003-1105-x
更新日期:2003-06-01 00:00:00