Recent advances in the purification, characterization and structure determination of lipases.

abstract：:Diets that contain high levels of n-3 fatty acids from fish oil have been shown to significantly effect macrophage cytolytic capacity, tumor necrosis factor alpha production and eicosanoid production. The present study was undertaken to determine whether n-3 fatty acids from vegetable origin [linseed oil (LIN)] would ...

journal_title：Lipids

authors： Hubbard NE,Chapkin RS,Erickson KL

更新日期：1994-09-01 00:00:00

abstract：:Plasma fatty acid (FA) composition is known to be an indicator of dietary fat quality, but the associations of other dietary factors with plasma FA composition remain unknown in children. We investigated the cross-sectional associations of food consumption with the proportions of FA and estimated desaturase activities...

journal_title：Lipids

authors： Venäläinen T,Schwab U,Ågren J,de Mello V,Lindi V,Eloranta AM,Kiiskinen S,Laaksonen D,Lakka TA

更新日期：2014-05-01 00:00:00

abstract：:The hypothesis that the essential oil from Tagetes minuta L. can interact with biological membranes was investigated by assessing its ability of perturbing the binding of a benzodiazepine [flunitrazepam (FNTZ)] to crude members from chick brains. The essential oil from T. minuta L. inhibited [3H]FNTZ specific binding ...

journal_title：Lipids

authors： García DA,Perillo MA,Zygadlo JA,Martijena ID

更新日期：1995-12-01 00:00:00

abstract：:Type 2 diabetes and dyslipidemia are risk factors for cardiovascular disease. However, mechanisms by which hypertriglyceridemia influences atherogenesis remain unclear. We examined effects of dyslipidemic diabetic serum on macrophage lipid accumulation as a model of foam cell formation. Normal human macrophages were c...

journal_title：Lipids

authors： Wong BX,Kyle RA,Myhill PC,Croft KD,Quinn CM,Jessup W,Yeap BB

更新日期：2011-10-01 00:00:00

abstract：:The effect of 4,4'-diethylamino-ethoxyhexestrol (DH) on acid esterase in rat liver was studied in vivo and in vitro. The acid esterase activity in the livers of rats treated with 0.125% DH for 1 week was found to decrease more than 60% as compared with that in untreated rats. The addition of DH to the incubation mediu...

journal_title：Lipids

authors： Kasama K,Yoshida K,Takeda S,Tsujimura R,Hasegawa S

更新日期：1976-10-01 00:00:00

abstract：:Strong perturbation of the ultraviolet spectrum of the tyrosines of porcine pancreatic colipase A is observed in the presence of Triton X-100 at concentration above the critical micellar concentration. Spectrophotometric titration of the phenolic groups of the protein shows that the apparent pKa value for two tyrosine...

journal_title：Lipids

authors： Canioni P,Julien R,Rathelot J,Sarda L

更新日期：1980-01-01 00:00:00

abstract：:A method was developed to analyze and quantify volatile fatty acids (VFA) such as acetic, propionic, butyric, isobutyric, valeric and isovaleric acids in biological specimens. To obtain good sample transfer into the chromatographic system an organic solvent had to be used together with an aqueous milieu, thus improvin...

journal_title：Lipids

authors： Fleming SE,Traitler H,Koellreuter B

更新日期：1987-03-01 00:00:00

abstract：:The term "conjugated linoleic acid" (CLA) refers to a group of positional and geometric isomers that are derived from linoleic acid and are found primarily in meat and milk products from ruminant animals. Due to the array of putative benefits associated with various forms of CLA, there has been recent interest in supp...

journal_title：Lipids

authors： Mosley SA,Shahin AM,Williams J,McGuire MA,McGuire MK

更新日期：2007-08-01 00:00:00

abstract：:This report deals with the fluorometric determination of fatty alcohols generated by the reduction of the ester linkage of lipids with NaBH4, and with the limitations of the reduction method for assaying oxidized lipids. Optimum conditions for the fluorometric analysis of primary and secondary alcohols using 1-anthroy...

journal_title：Lipids

authors： Nakamura T,Maeda H,Takahashi Y,Hama Y

更新日期：1990-09-01 00:00:00

abstract：:(8R)-Hydroperoxy-(9Z,12Z)-octadecadienoic acid (8-HPODE) is formed by aspergilli as an intermediate in biosynthesis of oxylipins with effects on sporulation. 8-HPODE is transformed by separate diol synthases to (5S,8R)-dihydroxy- and (8R,11S)-dihydroxy-(9Z,12Z)-octadecadienoic acids (5,8- and 8,11-DiHODE). The former ...

journal_title：Lipids

authors： Jernerén F,Oliw EH

更新日期：2012-07-01 00:00:00

abstract：:Pulmonary surfactant, the major surface-active component of which in the adult is dipalmitoylglycerophosphocholine, can be obtained by lavaging lungs with physiological saline. We have previously shown that there is an increase in the amount of phosphatidylcholine in fetal rabbit lung lavage during the latter part of ...

journal_title：Lipids

authors： Rooney SA,Gobran LI

更新日期：1977-12-01 00:00:00

abstract：:Plaques and white matter from brains of multiple sclerosis (MS) patients were analyzed for lipid content, class composition, and fatty acid composition of total lipid, together with the fatty acid composition of plaque glycerophospholipids, and the results were compared with white matter from normal brain. Plaques con...

journal_title：Lipids

authors： Wilson R,Tocher DR

更新日期：1991-01-01 00:00:00

abstract：:In offspring exposed in utero to a maternal diet high in fat (HF), we have previously demonstrated that despite similar birth weights, HF adult offspring at 6 months of age had significantly higher body weights, greater adiposity, and increased triacylglycerol (TAG) levels as compared to controls. We hypothesized that...

journal_title：Lipids

authors： Seet EL,Yee JK,Jellyman JK,Han G,Ross MG,Desai M

更新日期：2015-06-01 00:00:00

abstract：:The formation of hydroxy radicals, hexanal, and 2,4-decadienal was demonstrated from the autocatalytic dimer peroxide which had been reported by us in autoxidizing linoleate (Morita and Tokita in Lipids 41:91-95, 2006). Then, autoxidizing linoleate containing eleostearate was investigated for new autocatalytic substan...

journal_title：Lipids

authors： Morita M,Tokita M

更新日期：2008-07-01 00:00:00

abstract：:Mycobacterium fortuitum utilizes certain stereoisometric mixtures of individual multimethyl branched alkanes as sole carbon source, including 2,6(R), 10(S), 14(RS)-tetramethylhexadecane; 2.6(R), 10(S), 14(RS)-tetramethylheptadecane; 2,6(RS), 10(RS)-trimethyltetradecane, and 2,6(R), 10(S)-trimethylpentadecane. Products...

journal_title：Lipids

authors： Cox RE,Maxwell JR,Myers RN

更新日期：1976-01-01 00:00:00

abstract：:Considerable progress has been made in the selection and characterization of mutants that are defective in the synthesis of ergosterol in the yeast, Saccharomyces cerevisiae. Mutations in nearly every step of the yeast sterol biosynthetic pathway have been induced and selected. These mutants have been used to elucidat...

journal_title：Lipids

authors： Parks LW,Smith SJ,Crowley JH

更新日期：1995-03-01 00:00:00

abstract：:Feline omental lipid extracts, previously reported to be angiogenic in the cornea of rabbits, were fractionated and the major lipid components characterized. Approximately 97% of the chloroform/methanol extract consisted of triglycerides containing primarily 16:0, 18:0, 18:1 and 18:2 fatty acids. Trace quantities of f...

journal_title：Lipids

authors： McCluer RH,Evans JE,Williams M,Griffith AL,Catsimpoolas N

更新日期：1987-04-01 00:00:00

abstract：:After intracerebral injection of C(14)-ethanolamine into rats, the ethanolamine phosphoglycerides were isolated and hydrolyzed with mild alkali and acid. The specific radioactivity of the diacyl, acyl alkenyl, and acyl alkyl glyceryl-3-phosphorylethanolamine, the diacyl and acyl alkenyl glyceryl-3-phosphorylcholine, a...

journal_title：Lipids

authors： Horrocks LA,Ansell GB

更新日期：1967-07-01 00:00:00

abstract：:The conversion of labeled palmitic, linoleic, arachidonic and docosahexaenoic acids to their respective acyl CoA's was studied in homogenates and microsomes of rat tissues. The highest activity, both in homogenates and microsomes, was seen in liver and heart. There was moderate activity in retina, brain, lung, kidney ...

journal_title：Lipids

authors： Reddy TS,Bazan NG

更新日期：1984-12-01 00:00:00

abstract：:Plasma lipidomic studies using high performance liquid chromatography and mass spectroscopy offer detailed insights into metabolic processes. Taking the example of the most abundant plasma lipid class (phosphatidylcholines) we used the rich phenotypic and lipidomic data from the ongoing San Antonio Family Heart Study ...

journal_title：Lipids

authors： Kulkarni H,Meikle PJ,Mamtani M,Weir JM,Barlow CK,Jowett JB,Bellis C,Dyer TD,Johnson MP,Rainwater DL,Almasy L,Mahaney MC,Comuzzie AG,Blangero J,Curran JE

更新日期：2013-05-01 00:00:00

abstract：:The hypolipidemic drug clofibrate is known to affect the hepatic transport of various organic anions including bilirubin, fatty acids and sulfobromophthalein. Changes in the rate of metabolism and/or intracellular transport have been claimed responsible for the effect. To evaluate these possibilities, the transport of...

journal_title：Lipids

authors： Sorrentino D,Weisiger RA,Bass NM,Licko V

更新日期：1989-05-01 00:00:00

abstract：:The use of the organic cosolvents tetrahydrofuran and dimethylsulfoxide was found to be unsuitable for prostate tumor cell cultures because of solvent cytotoxicity and the poor solubility and instability of lycopene. For example, the half-life of lycopene in organic/aqueous solution was found to be less than 2 h. Ther...

journal_title：Lipids

authors： Xu X,Wang Y,Constantinou AI,Stacewicz-Sapuntzakis M,Bowen PE,van Breemen RB

更新日期：1999-10-01 00:00:00

abstract：:The molecular species in mixtures of phospholipid hydroperoxides are difficult to separate and identify by typical chromatographic and mass spectrometric techniques. As reported by Havrilla and coworkers, silver ion coordination ion-spray mass spectrometry (CIS-MS) has proven to be a powerful technique for the identif...

journal_title：Lipids

authors： Milne GL,Porter NA

更新日期：2001-11-01 00:00:00

abstract：:Dietary lipids, in particular unsaturated fat, promote the development of many experimental tumors. However, no mechanisms to fully explain these effects have been elucidated. Recent reports, which we summarize here, suggest a role for gap junction-mediated intercellular communication in the process of tumor promotion...

journal_title：Lipids

authors： Aylsworth CF,Welsch CW,Kabara JJ,Trosko JE

更新日期：1987-06-01 00:00:00

abstract：:Observational evidence suggests that in populations consuming low levels of n-3 highly unsaturated fatty acids, women have higher blood levels of docosahexaenoic acid (DHA; 22:3n-6) as compared with men. Increased conversion of alpha-linolenic acid (ALA; 18:3n-3) to DHA by females has been confirmed in fatty acid stab...

journal_title：Lipids

authors： Kitson AP,Stroud CK,Stark KD

更新日期：2010-03-01 00:00:00

abstract：:Various derivatives and isosteres of neolignans of the 2,5-diaryl tetrahydrofuran type have been synthesized as antagonists of platelet-activating factor (PAF). A detailed analysis of their structure-activity relationship (SAR) has revealed a clear preference for an asymmetrical molecular configuration with a high deg...

journal_title：Lipids

authors： Shen TY

更新日期：1991-12-01 00:00:00

abstract：:Skeletal muscle plays a major role in insulin-stimulated glucose disposal. This paper reviews the range of evidence in humans and experimental animals demonstrating close associations between insulin action and two major aspects of muscle morphology: fatty acid composition of the major structural lipid (phospholipid) ...

journal_title：Lipids

authors： Storlien LH,Pan DA,Kriketos AD,O'Connor J,Caterson ID,Cooney GJ,Jenkins AB,Baur LA

更新日期：1996-03-01 00:00:00

abstract：:Lipids from bovine optic nerve were analyzed. The total content of 16.5% by weight included 27.2% nonpolar lipids, 26.1% glycolipids, and 46.7% phospholipids by weight. Free cholesterol was the major component of the nonpolar lipid fraction. The cerebrosides, 73.5% of total glycolipids, were separated by thin layer ch...

journal_title：Lipids

authors： Das SK,Steen ME,McCullough MS,Bhattacharyya DK

更新日期：1978-10-01 00:00:00

abstract：:The effects of 5c,11c,14c-eicosatrienoic acid (20:3BSO) and 5c,11c,14c,17c-eicosatetraenoic acid (20:4BSO), polyunsaturated fatty acids (PUFA) contained in Biota orientalis seed oil (BSO), on lipid metabolism in rats were compared to the effects of fats rich in linoleic acid (LA) or alpha-linolenic acid (ALA) under si...

journal_title：Lipids

authors： Ikeda I,Oka T,Koba K,Sugano M,Lie Ken Jie MS

更新日期：1992-07-01 00:00:00

abstract：:Fatty acid alpha-hydroxylase from Sphingomonas paucimobilis is an unusual cytochrome P450 enzyme that hydroxylates the alpha-carbon of fatty acids in the presence of H2O2. Herein, we describe our investigation concerning the utilization of various substrates and the optical configuration of the alpha-hydroxyl product ...

journal_title：Lipids

authors： Matsunaga I,Sumimoto T,Ueda A,Kusunose E,Ichihara K

更新日期：2000-04-01 00:00:00