Abstract:
:Type 2 diabetes and dyslipidemia are risk factors for cardiovascular disease. However, mechanisms by which hypertriglyceridemia influences atherogenesis remain unclear. We examined effects of dyslipidemic diabetic serum on macrophage lipid accumulation as a model of foam cell formation. Normal human macrophages were cultured in media supplemented with 10% serum from non-diabetic normolipidemic or non-diabetic hypercholesterolemic adults versus adults with Type 2 diabetes; diabetes and hypertriglyceridemia; or diabetes and hypercholesterolemia. Exposure to diabetic sera resulted in increased macrophage fatty acids (2-3 fold higher, both saturated and unsaturated). Macrophage expression of CD36, scavenger receptor A (SR-A) and stearoyl-CoA desaturase (SCD) was increased, most prominently in macrophages exposed to hypertriglyceridemic diabetic serum (twofold increase in CD36 and fourfold increase in SCD, p < 0.05). In these conditions, RNA inhibition of CD36 reduced macrophage free cholesterol (163.9 ± 10.5 vs. 221.9 ± 26.2 mmol free cholesterol/g protein, p = 0.04). RNA inhibition of SCD decreased macrophage fatty acid content, increased ABCA1 level and enhanced cholesterol efflux (18.0 ± 3.9 vs. 8.0 ± 0.8% at 48 h, p = 0.03). Diabetic dyslipidemia may contribute to accelerated atherosclerosis via alterations in macrophage lipid metabolism favoring foam cell formation. Increased expression of CD36 and SR-A would facilitate macrophage lipid uptake, while increased expression of SCD could block compensatory upregulation of ABCA1 and cholesterol efflux. Further studies are needed to clarify whether modulation of macrophage lipid metabolism might reduce progression of diabetic atherosclerosis.
journal_name
Lipidsjournal_title
Lipidsauthors
Wong BX,Kyle RA,Myhill PC,Croft KD,Quinn CM,Jessup W,Yeap BBdoi
10.1007/s11745-011-3578-5subject
Has Abstractpub_date
2011-10-01 00:00:00pages
931-41issue
10eissn
0024-4201issn
1558-9307journal_volume
46pub_type
杂志文章相关文献
LIPIDS文献大全abstract::A cross-fostering design was used to examine the effects on brain and behavioral development in mice of pre- and/or postnatal dietary supplementation with n-3 fatty acids. Pregnant mice were fed either of two liquid diets, control (con) or experimental (exp). Each diet provided 3% of the calories in the form of n-6 fa...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544022
更新日期:1991-01-01 00:00:00
abstract::While there are many reports of studies that fed arachidonic acid (AA) to animals, there are very few reports of AA feeding to humans under controlled conditions. This 130-d study was conceived as a controlled, symmetrical crossover design with healthy, adult male volunteers. They lived in the metabolic research unit ...
journal_title:Lipids
pub_type: 临床试验,杂志文章
doi:10.1007/s11745-997-0054-8
更新日期:1997-04-01 00:00:00
abstract::Commercial preparations of Candida rugosa lipase (CRL) are mixtures of lipase isoforms used for the hydrolysis and synthesis of various esters. The presence of variable isoforms and the amount of lipolytic protein in the crude lipase preparations lead to a lack of reproducibility of biocatalytic reactions. Purificatio...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-004-1258-7
更新日期:2004-06-01 00:00:00
abstract::Sphingomyelin was isolated from cysts of the brine shrimp Artemia franciscana using QAE-Sephadex A25, Florisil and Iatrobeads column chromatographies. The chemical structure was identified using thin-layer chromatography, gas-liquid chromatography, infrared spectroscopy and matrix-assisted laser desorption ionization ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-010-3438-8
更新日期:2010-07-01 00:00:00
abstract::Plasma lipidomic studies using high performance liquid chromatography and mass spectroscopy offer detailed insights into metabolic processes. Taking the example of the most abundant plasma lipid class (phosphatidylcholines) we used the rich phenotypic and lipidomic data from the ongoing San Antonio Family Heart Study ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-013-3781-7
更新日期:2013-05-01 00:00:00
abstract::Levels of serum lipids and lipoproteins, and the fatty acid composition of plasma phospholipids, were measured in two genetically comparable, but widely separated, populations. The 1975 mortality rates for ischemic heart disease were significantly higher in one of these populations, the Manitoban residents of pure Ice...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537002
更新日期:1995-07-01 00:00:00
abstract::In the present paper, problems in connection with assay of the activity of magnesium-dependent rat liver phosphatidate phosphohydrolase (PAP) are discussed. PAP activity is usually measured by following the production of diacylglycerol or inorganic phosphate from the substrate phosphatidate. These two methods may give...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02522468
更新日期:1996-10-01 00:00:00
abstract::The aim of the present study was to characterize plasma lipids and lipoprotein cholesterol and glucose concentrations in hamsters fed either cis-9,trans-11 CLA (9c,11 tCLA); trans-10,cis-12 CLA (10t,12c CLA); or linoleic acid (LA) on the accumulation of aortic cholesterol in hypercholesterolemic hamsters. One hundred ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-5068-8
更新日期:2006-01-01 00:00:00
abstract::The total lipids, total neutral lipids, and total phospholipids from Aedes aegypti and aedes albopictus cells cultivated in vitro in a medium containing fetal calf serum were analyzed. The mosquito cells were harvested in the logarithmic and stationary phases of growth. The fatty acid profiles of the lipids showed dif...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532163
更新日期:1975-02-01 00:00:00
abstract::The relationship between LCAT mediated HDL modification and the redistribution of lipoprotein-unassociated apoA-IV to HDL was investigated in vitro. Immunoaffinity-isolated rat lipoprotein-unassociated apoA-IV was added to apoB-, apoE-, apoA-IV depleted, [3H]cholesterol labelled rat plasma and incubated at 37 degrees ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544075
更新日期:1989-12-01 00:00:00
abstract::We have studied the effect of various diets on the phospholipid fatty acid composition and in vitro delta 5 desaturase activity of hepatic microsomes derived either from the normal or streptozotocin-induced diabetic rat. The diets studied were the standard rat chow diet and a basal fat-free diet supplemented either wi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535763
更新日期:1989-10-01 00:00:00
abstract::The aim of this study was to determine the influence of long-term docosahexaenoic acid (DHA) dietary supplementation on the erythrocyte fatty acid profile and oxidative balance in soccer players after training and acute exercise. Fifteen volunteer male athletes (age 20.0 ± 0.5 years) were randomly assigned to a placeb...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3976-6
更新日期:2015-02-01 00:00:00
abstract::Changes in a number of morphological and biochemical parameters were observed in genetically obese Zucker rats and in lean controls between 3 and 58 weeks of age. By 3 weeks, the genetically obese rats had higher proportional (wt/100 g body wt) and absolute amounts of adipose tissue, hyperlipemia affecting all the lip...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535040
更新日期:1981-11-01 00:00:00
abstract::Intact washed human platelets aggregated in response to paf-acether (paf) and did not metabolize [3H]paf at concentrations up to 10 nM. However, when platelets were lysed by exposure to pH 9.5, resulting in 37.5 +/- 2.5% (mean +/- SD, n = 3) lactic dehydrogenase (LDH) release, 20.5 +/- 5.7% of the radioactivity was de...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536639
更新日期:1993-03-01 00:00:00
abstract::The purpose of the present investigation was to test whether permanent skeletal muscle cells (rat L6 cells) could serve as an in vitro model for alpha-tocopherol (alphaTocH) biodiscrimination studies. L6 cells were incubated in the presence of high density lipoprotein (HDL), low density lipoprotein (LDL), and very low...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0298-3
更新日期:1998-10-01 00:00:00
abstract::cis-5,cis-9-Octadecadienoic acid (2.7%) andcis-5,cis-9,cis-12-octadecatrienoic acid (24.9%) are present in the seed oil ofLarix leptolepis. The double bond positions were identified by ozonolysis and by gas chromatography-mass spectrometry of methoxy derivatives. Small quantities of branched chain acids of various cha...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532446
更新日期:1975-07-01 00:00:00
abstract::Previous studies show that long term variations in dietary fat consumption impact circulating fatty acid ethanolamide (FAE) concentrations, however, few studies have investigated short term effects of dietary fat feeding on FAE levels. The trial's objective was to explore the effect of acute feeding of varying amounts...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-017-4293-7
更新日期:2017-11-01 00:00:00
abstract::Membrane phospholipid (PL) composition has been shown to affect cellular function by altering membrane physical structure. The sarcolemma plasma membrane (SLpm) is integral to skeletal muscle function and health. Previous studies assessing SLpm PL composition have demonstrated contamination from transverse (t)-tubule,...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-013-3770-x
更新日期:2013-04-01 00:00:00
abstract::Lipases catalyze the hydrolysis and synthesis of triglycerides and their reactions are widely used in industry. The use of ionic liquids has been explored in order to improve their catalytic properties. However, the effect of these compounds on kinetic parameters of lipases has been poorly understood. A study of the k...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3973-9
更新日期:2015-01-01 00:00:00
abstract::In the presence of iodide and hydrogen peroxide, lactoperoxidase catalyzed the conversion of arachidonic acid into several iodinated products; the major one was previously identified as an iodo-delta-lactone. Two minor and less polar products have now been characterized as 15-iodo-14-hydroxyeicosatrienoic acid, omega-...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535024
更新日期:1981-04-01 00:00:00
abstract::The effects of conjugated linoleic acid (CLA) on the levels of chemical mediators in peritoneal exudate cells, spleen and lung, and the concentration of immunoglobulins in mesenteric lymph node and splenic lymphocytes and in serum were examined in rats. After feeding diets containing either 0 (control), 0.5 or 1.0% CL...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0236-4
更新日期:1998-05-01 00:00:00
abstract::Fatty acid composition of plasma phospholipids (PL), triglycerides (TG), and sterol esters (STE) was determined by high-resolution capillary gas-liquid chromatography in 22 obese children (age: 13.7 +/- 1.4 y, body weight relative to normal weight for height: 170 +/- 24%, mean +/- SD) and compared with data obtained i...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02529877
更新日期:1996-03-01 00:00:00
abstract::The beneficial roles of dietary fish oil in lowering serum TAG levels in animals and humans have been attributed in part to the high content of two n-3 polyunsaturated very long-chain FA, EPA, and DHA. Recent studies show that EPA induces mitochondrial beta-oxidation in hepatocytes, which might contribute to the syste...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-005-1443-8
更新日期:2005-08-01 00:00:00
abstract::Omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA) are essential components of the diet of all vertebrates. The major dietary source of n-3 LC-PUFA for humans has been fish and seafood but, paradoxically, farmed fish are also reliant on marine fisheries for fish meal and fish oil (FO), traditionally major ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-016-4191-4
更新日期:2016-10-01 00:00:00
abstract::Fast atom bombardment-tandem mass spectrometry was used to identify molecular species of paf-acether (paf) produced by human polymorphonuclear neutrophils. Using this biological material, normal phase high performance liquid chromatography was necessary prior to the fast atom bombardment-tandem mass spectrometry step....
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535248
更新日期:1989-02-01 00:00:00
abstract::Corn oil, peanut oil and randomized peanut oil exhibit different atherogenic potentials; peanut oil being more atherogenic than the other oils. This study was conducted to ascertain if the atherogenicity of these oils was related to their rates of lipolysis. Using both pancreatic lipase and milk lipoprotein lipase (LP...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534645
更新日期:1983-11-01 00:00:00
abstract::When fatty acids in fish are analyzed, results in percentage form (profile analysis) are mostly reported. However, the much more useful results expressed as mg/100 g (absolute analysis) is the main information required. Absolute methods based on calibration curves are of good accuracy but with a high degree of complex...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-012-3679-9
更新日期:2012-07-01 00:00:00
abstract::Flaxseed derivatives, including both oil and flax lignan, modify progression of renal injury in animal models, including Han:SPRD-cy polycystic kidney disease (PKD). Gender is a significant factor in the rates of progression of many forms of human renal disease, but the role of gender in the response to nutrition inte...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-5064-z
更新日期:2006-12-01 00:00:00
abstract::FA ethyl esters (FAEE) are nonoxidative metabolites of ethanol produced by the esterification of FA and ethanol. FAEE have been implicated as mediators of ethanol-induced organ damage in vivo and in vitro, and are markers of ethanol intake. Upon ethanol intake, FAEE are synthesized in the liver and pancreas in signifi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-1160-3
更新日期:2003-10-01 00:00:00
abstract::The effect of apo very low density lipoprotein (apo VLDL) and apoprotein C-II on the phospholipase A1 activity associated with lipoprotein lipase (E.C.3.1.1.3) was studied using purified bovine milk lipoprotein lipase. The enzyme degraded 14C phosphatidylcholine (PC) to 14C 2-acyl lysophosphatidylcholine at a rate of ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02540967
更新日期:1980-03-01 00:00:00