Lipase-based quantitation of triacylglycerols in cellular lipid extracts: requirement for presence of detergent and prior separation by thin-layer chromatography.

abstract：:Carbohydrate response element binding protein (ChREBP) regulates insulin-independent de novo lipogenesis. Recently, a novel ChREBPβ isoform was identified. The purpose of the current study was to define the effect of dietary carbohydrates (CHO) and obesity on the transcriptional activity of ChREBP isoforms and their r...

journal_title：Lipids

authors： Stamatikos AD,da Silva RP,Lewis JT,Douglas DN,Kneteman NM,Jacobs RL,Paton CM

更新日期：2016-01-01 00:00:00

abstract：:In this paper, evidence is presented on the capacity of Ehrlich ascites cells to synthesize in vitro monounsaturated fatty acids from radioactive palmitate. Localization of the double bond was determined by ozonolysis and subsequent reduction of the ozonides to aldesters followed by gas liquid chromatography. These re...

journal_title：Lipids

authors： Mercuri O,De Tomás ME,de Antueno RJ

更新日期：1981-12-01 00:00:00

abstract：:Bioassay-guided fractionation of a collection of Moorea bouillonii from Papua New Guinea led to the isolation of a new alkyl amide, mooreamide A (1), along with the cytotoxic apratoxins A-C and E. The planar structure of 1 was elucidated by NMR spectroscopy and mass spectrometry analysis. Structural homology between m...

journal_title：Lipids

authors： Mevers E,Matainaho T,Allara' M,Di Marzo V,Gerwick WH

更新日期：2014-11-01 00:00:00

abstract：:Normal phase high performance liquid chromatography methods are described for the separation of neutral lipid, fatty acid and five phospholipid classes using spectrophotometric detection at 206 nm. Separations were accomplished in less than 10 min for each lipid class. A mobile phase consisting of hexane/methyltertiar...

journal_title：Lipids

authors： Hamilton JG,Comai K

更新日期：1988-12-01 00:00:00

abstract：:Choline kinase was located in the cytosolic fractions of the filamentous, pathogenic fungi,Microsporum gypseum andEpidermophyton floccosum. A broad pH optima (6.0-9.0) was observed for theM. gypseum enzyme, but theE. floccosum enzyme was active at pH 8.4 as well as 10.5, the activity being higher at pH 8.4. Enzyme fro...

journal_title：Lipids

authors： Kasinathan C,Govindarajan U,Chopra A,Khuller GK

更新日期：1983-10-01 00:00:00

abstract：:The proximate and fatty acid compositions of the commercially important fish species (Engraulis encrasicolus, Alosa alosa, Belone belone, Scorpaena porcus, Pomatomus saltatrix, Mullus barbatus) from the Sinop region of the Black Sea were examined. The fat contents ranged from 1.26% (for scorpion fish) to 18.12% (for s...

journal_title：Lipids

authors： Kocatepe D,Turan H

更新日期：2012-06-01 00:00:00

abstract：:The positional distribution of 22ratio6 and 22ratio5 in aquatic animal triglycerides can be predicted by simple proportionality equations of the typey=kx. The mole % 22ratio6 at the 1, 2, or 3 position (y) is obtained by multiplying the proportionality constant for that position (k (1),k (2), ork (3)) times the mole %...

journal_title：Lipids

authors： Litchfield C

更新日期：1968-09-01 00:00:00

abstract：:Fatty acid synthetase purified (20 times) from lactating bovine mammary tissue had an approximate mot wt of 485,000. The enzyme had a high content of acidic and hydrophobic amino acid residues; 62±4 sulhydryl groups and one 4' phosphopantetheine residue/mole of enzyme. The enzyme was relatively stable when stored (3 m...

journal_title：Lipids

authors： Kinsella JE,Bruns D,Infante JP

更新日期：1975-04-01 00:00:00

abstract：:The effects of increasing concentrations of eicosapentaenoic acid (20:5n-3; EPA) and oleic acid (18:1n-9; OA) on esterification to triacylglycerols (TG) and phospholipids (PL), and the relationship to formation and secretion of the very low density lipoproteins (VLDL) were compared in the isolated perfused rat liver. ...

journal_title：Lipids

authors： Zhang ZJ,Wilcox HG,Castellani L,Fungwe TV,Elam MB,Heimberg M

更新日期：1993-05-01 00:00:00

abstract：:The biosynthesis of [14C] CDP-diglyceride was studied using rat liver microsomes which were endogenously labeled with [14C] phosphatidic acid by preincubation of unlabeled microsomes with sn-[14C] glycerol-3-phosphate and appropriate cofactors. The formation of CDP-diglyceride from radioactive phosphatidate showed an ...

journal_title：Lipids

authors： Holub BJ,Piekarski J

更新日期：1976-04-01 00:00:00

abstract：:Vegetable oils are an extremely important agricultural commodity. Their use has risen inexorably for the last 50 years and will undoubtedly be even more prevalent in the future. They have a role not only in foodstuffs but also as renewable chemicals. However, our understanding of their metabolism, and particularly its...

journal_title：Lipids

authors： Chen G,Woodfield HK,Pan X,Harwood JL,Weselake RJ

更新日期：2015-11-01 00:00:00

abstract：:Intact washed human platelets aggregated in response to paf-acether (paf) and did not metabolize [3H]paf at concentrations up to 10 nM. However, when platelets were lysed by exposure to pH 9.5, resulting in 37.5 +/- 2.5% (mean +/- SD, n = 3) lactic dehydrogenase (LDH) release, 20.5 +/- 5.7% of the radioactivity was de...

journal_title：Lipids

authors： Korth R,Bidault J,Palmantier R,Benveniste J,Ninio E

更新日期：1993-03-01 00:00:00

abstract：:We have examined the possibility that human polymorphonuclear cells exposed to IgA immune complexes can mediate the production of platelet-activating factor (PAF) and oxygen radicals. We found that human IgA and IgG immune aggregates stimulated, to a similar extent, PAF and O2- production by human polymorphonuclear ce...

journal_title：Lipids

authors： Hernando P,Egido J,de Nicolas R,Gonzalez E

更新日期：1991-12-01 00:00:00

abstract：:The stereochemistry of fat digestion and absorption was investigated in rats with thoracic duct fistulas, after feeding synthetic triacylglycerol or alkyldiacylglycerol. After feeding 1,2-dilauroyl-3-oleoyl-sn-glycerol, dilauroyloleoylglycerol and lauroyldioleoylglycerol were the most abundant chyle triacylglycerols. ...

journal_title：Lipids

authors： Akesson B,Gronowitz S,Herslof B,Ohlson R

更新日期：1978-05-01 00:00:00

abstract：:A sucrose-rich diet (SRD) induces insulin resistance and dyslipidemia with impaired hepatic glucose production and gluconeogenesis, accompanied by altered post-receptor insulin signaling steps. The aim of this study was to examine the effectiveness of fish oil (FO) to reverse or improve the impaired hepatic glucose me...

journal_title：Lipids

authors： Hein GJ,Chicco A,Lombardo YB

更新日期：2012-02-01 00:00:00

abstract：:The effects of SMP-500, a novel ACAT inhibitor, on serum lipid levels, hepatic lipid secretion rate, and hepatic lipid disposition in rats were studied to clarify its lipid-lowering action. SMP-500 reduced the serum cholesterol level in a dose-dependent manner in rats fed a hypercholesterolemic diet. SMP-500 also redu...

journal_title：Lipids

authors： Ioriya K,Nishimura T,Ohashi N

更新日期：2002-04-01 00:00:00

abstract：:The term "conjugated linoleic acid" (CLA) refers to a group of positional and geometric isomers that are derived from linoleic acid and are found primarily in meat and milk products from ruminant animals. Due to the array of putative benefits associated with various forms of CLA, there has been recent interest in supp...

journal_title：Lipids

authors： Mosley SA,Shahin AM,Williams J,McGuire MA,McGuire MK

更新日期：2007-08-01 00:00:00

abstract：:Cholesteryl esterase activities were determined in homogenates of rat heart (ventricles), isolated, calcium-tolerant, cardiac myocytes and aortic tissue and were compared with acid and neutral triglyceride lipase activities in these fractions. Using cholesteryl oleate/phosphatidylcholine/taurocholate emulsions and dig...

journal_title：Lipids

authors： Stam H,Broekhoven-Schokker S,Schoonderwoerd K,Hülsmann WC

更新日期：1987-02-01 00:00:00

abstract：:The anesthetic potency of four derivatives of n-octane was measured by tadpole righting reflex and expressed as effective millimolar concentration of drug in membrane, EDM50. Potency diminished (ED50 increased) in this order: 1-octanol, EDM50 = 5.5; 1-(2-methoxyethoxy)octane, EDM50 = 28; 1-methoxyoctane, EDM50 = 61; a...

journal_title：Lipids

authors： Brockerhoff H,Brockerhoff S,Box LL

更新日期：1986-06-01 00:00:00

abstract：:The minimum requirement of linoleic acid to prevent effects of dietary C18 trans fatty acids on eicosanoid biosynthesis in rats was assessed. In a first experiment, six groups of animals were fed diets with a high content of trans fatty acids [20% of energy (en%)], and increasing amounts of linoleic acid (0.4 to 7.1 e...

journal_title：Lipids

authors： Zevenbergen JL,Haddeman E

更新日期：1989-06-01 00:00:00

abstract：:The sn-position of FA in membrane lipids has an influence on the physiological function of cells, is predictive for diseases, and therefore is useful for diagnostics. The current study compares the compositions of acyl chain substituents in the sn-1 and sn-2 positions of the glycerol backbones of phospholipids derived...

journal_title：Lipids

authors： Beermann C,Möbius M,Winterling N,Schmitt JJ,Boehm G

更新日期：2005-02-01 00:00:00

abstract：:Many reports have shown the beneficial effects of consumption of pine seeds and pine seed oil. However, few studies have examined the biological effect of pinolenic acid (PNA; 5,9,12-18:3), the main fatty acid in pine seed oil. In this study, using murine macrophage RAW264.7 cells as a model, we examined the effect of...

journal_title：Lipids

authors： Chuang LT,Tsai PJ,Lee CL,Huang YS

更新日期：2009-03-01 00:00:00

abstract：:Although hypocholesterolemia is a reported finding in sickle cell disease (SCD), low-density lipoprotein (LDL)/high-density lipoprotein (HDL) subfractions and HDL-associated enzymes have not been determined in SCD patients. Blood was collected from 38 hemoglobin (Hb)A volunteers and 45 homozygous HbSS patients who had...

journal_title：Lipids

authors： Ozturk OH,Can Y,Yonden Z,Motor S,Oktay G,Kaya H,Aslan M

更新日期：2013-12-01 00:00:00

abstract：:To examine whether dietary fat alters membrane lipid composition and peroxidation of polyunsaturated fatty acids in "non-proliferative" and "proliferative" cells in the large intestine, Sprague-Dawley rats were fed diets providing a polyunsaturated-to-saturated fatty acid ratio of 1.2 or 0.3 at a high or low level of ...

journal_title：Lipids

authors： Turini ME,Thomson AB,Clandinin MT

更新日期：1991-06-01 00:00:00

abstract：:The effects of 5c,11c,14c-eicosatrienoic acid (20:3BSO) and 5c,11c,14c,17c-eicosatetraenoic acid (20:4BSO), polyunsaturated fatty acids (PUFA) contained in Biota orientalis seed oil (BSO), on lipid metabolism in rats were compared to the effects of fats rich in linoleic acid (LA) or alpha-linolenic acid (ALA) under si...

journal_title：Lipids

authors： Ikeda I,Oka T,Koba K,Sugano M,Lie Ken Jie MS

更新日期：1992-07-01 00:00:00

abstract：:Microsomal and myelin membrane fractions were prepared from the brains of warm-adapted (room temperature) and hibernating Syrian Hamsters (Mesocricetus auratus). Ethanolamineglycerophospholipids were isolated and subjected to a fractionation scheme to separate the fatty acids of the plasmenylethanolamine and the phosp...

journal_title：Lipids

authors： Blaker WD,Moscatelli EA

更新日期：1979-12-01 00:00:00

abstract：:The seed oil ofEuonymus verrucosus Scop., family Celastraceae, contains more than 90% 1,2-diacyl-3-acetins (monoacetotriglycerides). The constituent triglyceride acids, other than acetic, are the usual long-chain fatty acids.Thin-layer chromatography (TLC), infrared (IR) and hydrolysis with pancreatic lipase indicate ...

journal_title：Lipids

authors： Kleiman R,Miller RW,Earle FR,Wolff IA

更新日期：1967-11-01 00:00:00

abstract：:The cholesterol-suppressive action of the tocotrienol-rich-fraction (TRF) of palm oil may be due to the effect of its constituent tocotrienols on beta-hydroxy-beta-methylglutaryl coenzyme A (HMG-CoA) reductase activity. The tocotrienols, modulate HMG-CoA reductase activity via a post-transcriptional mechanism. As a co...

journal_title：Lipids

authors： Yu SG,Thomas AM,Gapor A,Tan B,Qureshi N,Qureshi AA

更新日期：2006-05-01 00:00:00

abstract：:The schweinfurthins have potent antiproliferative activity in multiple glioblastoma multiforme (GBM) cell lines; however, the mechanism by which growth is impeded is not fully understood. Previously, we demonstrated that the schweinfurthins reduce the level of key isoprenoid intermediates in the cholesterol biosynthet...

journal_title：Lipids

authors： Kuder CH,Weivoda MM,Zhang Y,Zhu J,Neighbors JD,Wiemer DF,Hohl RJ

更新日期：2015-12-01 00:00:00

abstract：:Orally administered dihomo-gamma-linolenic acid (DHLA) is well absorbed in man; it appears in blood after ca. 4 hr first as triglyceride ester and later as phospholipid. After sustained-dosing, DHLA penetrated membrane pools and all phospholipid components but, depending on the dosage, reached a metabolic equilibrium ...

journal_title：Lipids

authors： Stone KJ,Willis AL,Hart WM,Kirtland SJ,Kernoff PB,McNicol GP

更新日期：1979-02-01 00:00:00