Abstract:
:Lipoproteins from the plasma of three nonlactating Holstein cows were isolated using either preparative ultracentrifugation or gel filtration chromatography. Lipoprotein classes obtained by ultracentrifugation were very low density plus chylomicra, less than 1.006 g/ml; low density, 1.007-1.039 g/ml; high density1, 1.040-1.063 g/ml; and high density, 1.064-1.22 g/ml. These lipoprotein classes were individually applied to an agarose gel column to determine at what volume they eluted in comparison to lipoproteins that were separated after applying total bovine lipoproteins to the column. Three major peaks corresponding to very low density lipoproteins plus chylomicra, low density, and high density lipoproteins resulted after gel filtration of total lipoproteins. Very low density lipoproteins plus chylomicra, obtained by ultracentrifugation, eluted as a single peak, as did low density and high density lipoproteins. However, high density1 lipoproteins eluted as two peaks. The first peak eluted at the same volume as low density lipoproteins, and the second peak eluted at a volume similar to that of the ascending slope of the high density lipoprotein peak. Results from disc polyacrylamide gel electrophoresis, immunoelectrophoresis and double immunodiffusion of lipoprotein fractions, and SDS polyacrylamide gel electrophoresis of their apoproteins, similarly indicated that the lipoproteins present in the 1.040-1.063 g/ml density interval are a mixture of low and high density lipoproteins rather than a unique class of lipoproteins.
journal_name
Lipidsjournal_title
Lipidsauthors
Grummer RR,Davis CL,Hegarty HMdoi
10.1007/BF02534638subject
Has Abstractpub_date
1983-11-01 00:00:00pages
795-802issue
11eissn
0024-4201issn
1558-9307journal_volume
18pub_type
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