Differences in the phospholipid, cholesterol, and fatty acyl composition of 3T3 and SV3T3 plasma membranes.

Abstract:

:An analysis of the phospholipid, cholesterol, and phospholipid fatty acyl composition of isolated plasma membranes of 3T3 and SV3T3 mouse mebryo cells has been performed. The results show that the plasma membrane of SV3T3 cells contain relatively less phosphatidylethanolamine and sphingomyelin and more cholesterol than 3T3 plasma membranes. The fatty acyl composition of individual phospholipid classes as determined by gas liquid chromatography also showed differences between 3T3 and SV3T3 plasma membranes. The plasma membranes of SV40 transformed 3T3 cells contain: (a) a higher percentage of 18:1 and less 20:3 and 20:4 in phosphatidylethanolamine; (b) a higher percentage of 18:1 in phosphatidylserine; and (c) a higher percentage of 18:2 and 20:4 in phosphatidylinositol.

journal_name

Lipids

journal_title

Lipids

authors

Perkins RG,Scott RE

doi

10.1007/BF02533741

subject

Has Abstract

pub_date

1978-10-01 00:00:00

pages

653-7

issue

10

eissn

0024-4201

issn

1558-9307

journal_volume

13

pub_type

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