Abstract:
:Mice exposed to cold rely upon brown adipose tissue (BAT)-mediated nonshivering thermogenesis to generate body heat using dietary glucose and lipids from the liver and white adipose tissue. In this report, we investigate how cold exposure affects the PI3 K/Akt signaling cascade and the expression of genes involved in lipid metabolism and trafficking in BAT. Cold exposure at an early time point led to the activation of the PI3 K/Akt, insulin-like signaling cascade followed by a transient decrease in adipose triglyceride lipase (ATGL) gene and protein expression in BAT. To further investigate how cold exposure-induced signaling altered ATGL expression, cultured primary brown adipocytes were treated with the beta(3)-adrenergic receptor (beta(3)AR) agonist CL 316,243 (CL) resulting in activation of PI3 K/Akt, ERK 1/2, and p38 signaling pathways and significantly decreased ATGL protein levels. ATGL protein levels decreased significantly 30 min post CL treatment suggesting protein degradation. Inhibition of PKA signaling by H89 rescued ATGL levels. The effects of PKA signaling on ATGL were shown to be independent of relevant pathways downstream of PKA such as PI3 K/Akt, ERK 1/2, and p38. However, CL treatment in 3T3-L1 adipocytes did not decrease ATGL protein and mRNA expression, suggesting a distinct response in WAT to beta3-adrenergic agonism. Transitory effects, possibly attributed to acute Akt activation during the early recruitment phase, were noted as well as stable changes in gene expression which may be attributed to beta3-adrenergic signaling in BAT.
journal_name
Lipidsjournal_title
Lipidsauthors
Deiuliis JA,Liu LF,Belury MA,Rim JS,Shin S,Lee Kdoi
10.1007/s11745-010-3422-3subject
Has Abstractpub_date
2010-06-01 00:00:00pages
479-89issue
6eissn
0024-4201issn
1558-9307journal_volume
45pub_type
杂志文章相关文献
LIPIDS文献大全abstract::Milk fatty acids consist of about 20-25% palmitic acid (16:0), with about 70% of 16:0 esterified to the sn-2 position of the milk triacylglycerols. Hydrolysis of dietary triacylglycerols by endogeneous lipases produces sn-2 monoacylglycerols and free fatty acids, which are absorbed, reesterified, and then secreted int...
journal_title:Lipids
pub_type: 临床试验,杂志文章
doi:10.1007/BF02536625
更新日期:1994-08-01 00:00:00
abstract::Over 50 years of research has sought to define the role dietary fat plays in cardiovascular disease (CVD) risk. Although optimal dietary fat quantity has been keenly pursued over past decades, attention has recently centered on the value of dietary fat quality. The purpose of the present review is to provide a critica...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-010-3524-y
更新日期:2011-03-01 00:00:00
abstract::The phospholipid composition of gill tissue from thermally acclimated rainbow trout, Salmo gairdneri, was determined by Iatroscan analysis following an initial development of the chromarods in a non-polar solvent to remove neutral lipids. Standard curves for all phospholipids, although linear through most of the conce...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534892
更新日期:1985-08-01 00:00:00
abstract::Strong perturbation of the ultraviolet spectrum of the tyrosines of porcine pancreatic colipase A is observed in the presence of Triton X-100 at concentration above the critical micellar concentration. Spectrophotometric titration of the phenolic groups of the protein shows that the apparent pKa value for two tyrosine...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534109
更新日期:1980-01-01 00:00:00
abstract::The prevalence of colorectal cancer (CRC) continues to increase. Treatment of CRC remains a significant clinical challenge, and effective therapies for advanced CRC are desperately needed. Increasing attention and ongoing research efforts have focused on krill oil that may provide health benefits to the human body. He...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12281
更新日期:2020-09-15 00:00:00
abstract::Diacylglycerol acyltransferase (DGAT) catalyzes the final step of triacylglycerol (TAG) synthesis, and is considered as a potential target to control hypertriglyceridemia or other metabolic disorders. In this study, we found that the extract of rose petals suppressed TAG synthesis in cultured cells, and that the extra...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-011-3559-8
更新日期:2011-08-01 00:00:00
abstract::Enrichment of beef muscle with n-3 fatty acids (FA) is one means to introduce these FA into the diet, but ruminal biohydrogenation limits their bioavailability. To address this problem, we evaluated the ability of condensed tannin (quebracho), in the presence or absence of casein, to protect 18:3n-3 in flaxseed from h...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-007-3126-5
更新日期:2007-12-01 00:00:00
abstract::We evaluated the effects of dietary lipid class (phospholipid vs. neutral lipid) and level of n-3 long-chain PUFA (LC-PUFA) on the growth, digestive enzymatic activity, and histological organization of the intestine and liver in European sea bass larvae. Fish were fed from the onset of exogenous feeding at 7 to 37 d p...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-005-1422-0
更新日期:2005-06-01 00:00:00
abstract::Docosahexaenoic acid (DHA), a prominent long-chain fatty acid of the omega-3 family, is present at high amount in brain tissues, especially in membrane phospholipids. This polyunsaturated fatty acid is the precursor of various oxygenated lipid mediators involved in diverse physiological and pathophysiological processe...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12006
更新日期:2018-01-01 00:00:00
abstract::The kinetics of in vivo clearance of apolipoprotein (apo) A-I radioiodinated by the iodine monochloride (ICI) method of McFarlane [McFarlane, A.S. (1958) Efficient Trace-Labelling of Proteins with Iodine, Nature 182, 53] as modified by Bilheimer and co-workers [Bilheimer, D.W., Eisenberg, S., and Levy, R.I. (1972) The...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-003-1053-5
更新日期:2003-03-01 00:00:00
abstract::The concentrations of nonesterified fatty acids and glycerol in rat parametrial adipose tissue increased at peak lactation. Adipose tissue from lactating rats showed higher rates of release of nonesterified fatty acids and glycerol when incubated in vitro than did tissue from nonlactating rats, but there was a substan...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532850
更新日期:1976-05-01 00:00:00
abstract::Dietary fatty acids have different effects on fat deposition in pigs. To clarify the underlying mechanisms of this difference, we compared the metabolism of palmitic (PAM, saturated), oleic (OLA, monounsaturated) and linoleic acid (LNA, polyunsaturated) in porcine adipocytes treated with 100 μM PAM, OLA or LNA. We obs...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-017-4302-x
更新日期:2017-11-01 00:00:00
abstract::Plaques and white matter from brains of multiple sclerosis (MS) patients were analyzed for lipid content, class composition, and fatty acid composition of total lipid, together with the fatty acid composition of plaque glycerophospholipids, and the results were compared with white matter from normal brain. Plaques con...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544017
更新日期:1991-01-01 00:00:00
abstract::The effects of growth temperature on the fatty acid compositions of the phosphatidylcholine (PC), phosphatidylethanolamine (PE), and total lipid (TL) fractions of the free-living nematode Caenorhabditis elegans were investigated. A reduction in growth temperature from 25 to 15 degrees C caused the proportions of eicos...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02524292
更新日期:1996-11-01 00:00:00
abstract::A 10-hydroxy stearic acid-containing lipid from Cryptosporidium parvum was purified by thin-layer chromatography and analyzed by infrared spectroscopy, fast-atom bombardment mass spectrometry, 1H and 31P nuclear magnetic resonance spectroscopy, and was identified as phosphatidyl-ethanolamine. ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-997-0102-4
更新日期:1997-07-01 00:00:00
abstract::The purpose of the present investigation was to test whether permanent skeletal muscle cells (rat L6 cells) could serve as an in vitro model for alpha-tocopherol (alphaTocH) biodiscrimination studies. L6 cells were incubated in the presence of high density lipoprotein (HDL), low density lipoprotein (LDL), and very low...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0298-3
更新日期:1998-10-01 00:00:00
abstract::The minimum requirement of linoleic acid to prevent effects of dietary C18 trans fatty acids on eicosanoid biosynthesis in rats was assessed. In a first experiment, six groups of animals were fed diets with a high content of trans fatty acids [20% of energy (en%)], and increasing amounts of linoleic acid (0.4 to 7.1 e...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535138
更新日期:1989-06-01 00:00:00
abstract::Uptake of oxidized low-density lipoprotein by endothelial cells is a critical step for the initiation of atherosclerosis. Triacylglycerol uptake in these cells is understood to be a part of the process. The present investigation, comparison among the effects of simple acylglycerol, including tristearin, triolein, and ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3889-4
更新日期:2014-05-01 00:00:00
abstract::The pulp from unerupted calves' teeth was found to contain low levels of glycerol ester hydrolase activity. Solutions in Tris buffer cleared tributyrylglycerol agar and hydrolyzed emulsified olive oil. The average quantity of free fatty acids released in five assays with olive oil was: 2.8, S.D.+/-1.4. Specific activi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533302
更新日期:1977-02-01 00:00:00
abstract::Arachidonic acid was reduced by hydrazine to yield isomeric eicosatrienoic acids with other products. Methylcis,cis,cis-5,8,11-eicosatrienoate was isolated from the products by silver ion chromatography and preparative gas liquid chromatography in 8% yield. The structure was confirmed by spectral studies and oxidative...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534947
更新日期:1982-04-01 00:00:00
abstract::Ceramides are a family of signalling lipids with diverse physiological functions that include pro-differentiative and pro-apoptotic signalling. Ceramides and their derivatives are major constituents of myelin, maintaining neuronal conductivity. Ceramides are synthesized by ceramide synthases, of which there are six is...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3969-5
更新日期:2015-01-01 00:00:00
abstract::In an attempt to combine the hypocholesterolemic properties of plant sterols with the hypotriglyceridemic action of fish oil FA, plant sterols have recently been esterified to fish oil n-3 PUFA. The objective of this study was to determine the effects of plant sterols esterified to n-3 PUFA on plasma lipid levels and ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-005-1432-y
更新日期:2005-07-01 00:00:00
abstract::Linoleic acid was efficiently converted into the two major components of conjugated linoleic acid, 9Z,11E-octadecadienoic (1a) and 10E,12Z-octadecadienoic acid (1b) using either the superbase (n-butyllithium/potassium tert-butoxide) or by simply refluxing with KOH in 1-butanol. In turn, 1a and 1b were separated from e...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-999-0436-y
更新日期:1999-08-01 00:00:00
abstract::Male weanling rats were fed semi-synthetic diets high in saturated fat (beef tallow) vs high in linoleic acid (safflower oil) with or without high levels of alpha-linolenic acid (linseed oil) for a period of 28 days. The effect of feeding these diets on cholesterol content and fatty acid composition of serum and liver...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535173
更新日期:1989-04-01 00:00:00
abstract::Orally administered dihomo-gamma-linolenic acid (DHLA) is well absorbed in man; it appears in blood after ca. 4 hr first as triglyceride ester and later as phospholipid. After sustained-dosing, DHLA penetrated membrane pools and all phospholipid components but, depending on the dosage, reached a metabolic equilibrium ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533869
更新日期:1979-02-01 00:00:00
abstract::Hepatocytes isolated from rainbow trout fed on diets containing either fish oil or olive oil were incubated with individual odd-chain length unsaturated fatty acids (19:1n-9, 19:2n-6, 19:3n-3, 21:2n-6, 21:3n-6, 21:4n-6, 21:3n-3, and 21:5n-3) to examine whether these fatty acids were substrates for modification by desa...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-997-0078-0
更新日期:1997-06-01 00:00:00
abstract::In the wild, nutrients from plants provide for the requirements of all herbivores and ultimately all animals, yet little work has been published on how such nutrients change with environmental conditions. Plant lipids are nutritionally important for browsing mammals, initially with respect to taste and palatability, b...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0186-x
更新日期:1998-01-01 00:00:00
abstract::A procedure for the preparation of specific molecular species of D-erythro-lactosylceramide involving deacylation and reacylation of lactosylceramide prepared from bovine brain gangliosides is described. Lactosylceramide was N-deacylated by alkaline hydrolysis and the resulting four lysolactosylceramides, which contai...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02538917
更新日期:1994-10-01 00:00:00
abstract::Type 2 diabetes and dyslipidemia are risk factors for cardiovascular disease. However, mechanisms by which hypertriglyceridemia influences atherogenesis remain unclear. We examined effects of dyslipidemic diabetic serum on macrophage lipid accumulation as a model of foam cell formation. Normal human macrophages were c...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-011-3578-5
更新日期:2011-10-01 00:00:00
abstract::A method for the simultaneous quantitative analysis of prostaglandin E2 (PGE2) and PGE3 is described. The PG were analyzed by selected ion monitoring as the methyl ester-TMS ether derivatives of PGB2 and PGB3, respectively. The internal standard for the quantification of both species was [3,3,4,4-2H4]PGE2. A linear re...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535360
更新日期:1982-11-01 00:00:00