Evaluation of apolipoprotein A-I kinetics in rabbits in vivo using in situ and exogenous radioiodination methods.


:The kinetics of in vivo clearance of apolipoprotein (apo) A-I radioiodinated by the iodine monochloride (ICI) method of McFarlane [McFarlane, A.S. (1958) Efficient Trace-Labelling of Proteins with Iodine, Nature 182, 53] as modified by Bilheimer and co-workers [Bilheimer, D.W., Eisenberg, S., and Levy, R.I. (1972) The Metabolism of Very Low Density Lipoprotein Proteins. I. Preliminary in vitro and in vivo Observations, Biochim. Biophys. Acta 260, 212-221] and by using the IODO Beads Iodination Reagent were evaluated in rabbits. Both human apoA-I and rabbit HDL radioiodinated by the IODO Beads Iodination Reagent were cleared faster from plasma of rabbits than those radiolabeled by the ICI method. However, the different radiolabeling procedures in the ICI method, i.e., apoA-I radiolabeled either exogenously or in situ as a part of intact HDL, were not associated with a significant difference in the in vivo kinetics of apoA-I in rabbits if apoA-I was prepared by the guanidine HCI method and used fresh. 125I-ApoA-I subjected to delipidation and lyophilization was cleared only slightly faster from the plasma of rabbits than fresh 125I-apoA-I. We also found that apoA-I separated by the guanidine HCI method and used fresh was cleared faster from the plasma of rabbits when it was injected as free apoA-I without adding serum albumin or after in vitro incubation with rabbit HDL than when injected after reassociation with rabbit plasma. We conclude that the ICI method is a more appropriate radioiodination method for studying the in vivo kinetics of HDL than the IODO Beads Iodination Reagent and that the in vitro incubation conditions before injection are important factors that affect the in vivo kinetics of apo A-I.






Zhang B,Shimoji E,Tanaka H,Saku K




Has Abstract


2003-03-01 00:00:00














  • HPLC analysis of desmosterol, 7-dehydrocholesterol, and cholesterol.

    abstract::A simple and sensitive method to analyze mixtures of desmosterol, 7-dehydrocholesterol and cholesterol is described. The method involves the oxidative conversion of the sterols with cholesterol oxidase, followed by high performance liquid chromatographic (HPLC) analysis. A C18 reversed phase column (3 microns, 75 X 4....


    pub_type: 杂志文章


    authors: Goh EH,Colles SM,Otte KD

    更新日期:1989-07-01 00:00:00

  • Dietary linoleic, alpha-linolenic and oleic acids are oxidized at similar rates in rats fed a diet containing these acids in equal proportions.

    abstract::The objective of this study was to examine whether whole body oxidation rates of dietary linoleic, alpha-linolenic and oleic acids differ when the acids are provided in identical quantities. Male rats were fed for 10 wk a 15% fat (w/w) diet containing equal amounts of linoleic, alpha-linolenic and oleic acids (22.7, 2...


    pub_type: 杂志文章


    authors: Jones PJ

    更新日期:1994-07-01 00:00:00

  • Initiation of lipid peroxidation by a peroxidase/hydrogen peroxide/halide system.

    abstract::A lactoperoxidase/H2O2/halide system caused the initiation of linoleate peroxidation as indicated by diene conjugation. Coupled lipid peroxidation was accelerated by iodide, chloride and bromide ions at pH 4.0 and 6.2. No peroxidation occurred in the presence of H2O2 or lactoperoxidase alone. The rate of linoleate per...


    pub_type: 杂志文章


    authors: Kanner J,Kinsella JE

    更新日期:1983-03-01 00:00:00

  • Lipid and fatty acid compositions of a novel docosahexaenoic acid-producing marine bacterium.

    abstract::An unidentified bacterial strain, SCRC-21406, isolated from the intestine of a marine fish, Glossanodon semifasciatus, produced docosahexaenoic acid at 23% (mol/mol) [= 28% (w/w)] of total fatty acids in a medium containing 0.5% (wt/vol) peptone and 0.1% (wt/vol) yeast extract at 12 degrees C under atmospheric pressur...


    pub_type: 杂志文章


    authors: Watanabe K,Ishikawa C,Ohtsuka I,Kamata M,Tomita M,Yazawa K,Muramatsu H

    更新日期:1997-09-01 00:00:00

  • Structures of the ceramides from porcine palatal stratum corneum.

    abstract::Ceramides are the major type of lipid found in stratum corneum from the skin, gingiva and hard palate. The present study examined the ceramides of the stratum corneum from the hard palate. Six fractions of ceramides were isolated by preparative thin-layer chromatography. The least polar fraction contained an unusual a...


    pub_type: 杂志文章


    authors: Hill JR,Wertz PW

    更新日期:2009-03-01 00:00:00

  • Hempseed Products Fed to Hens Effectively Increased n-3 Polyunsaturated Fatty Acids in Total Lipids, Triacylglycerol and Phospholipid of Egg Yolk.

    abstract::Hempseed products represent potential alternative feed ingredients for poultry. However, their usage is not currently approved due to a lack of data to support their safety and efficacy. In this regard, the current study was conducted to assess the impact of dietary concentration of hempseed (HS) products and duration...


    pub_type: 杂志文章


    authors: Neijat M,Suh M,Neufeld J,House JD

    更新日期:2016-05-01 00:00:00

  • Effects of different fractions of the barley kernel on the hepatic lipid metabolism of chickens.

    abstract::Various physical fractions of the barley kernel were fed to one-day-old female and male chickens to determine their effect on hepatic beta-hydroxy-beta-methylglutaryl coenzyme A (HMG-CoA) reductase, cholesterol 7 alpha-hydroxylase and the lipogenic enzymes, acetyl-CoA carboxylase (ACX), malic enzyme (ME), citrate-clea...


    pub_type: 杂志文章


    authors: Burger WC,Qureshi AA,Prentice N,Elson CE

    更新日期:1982-12-01 00:00:00

  • Elevated production of docosahexaenoic acid in females: potential molecular mechanisms.

    abstract::Observational evidence suggests that in populations consuming low levels of n-3 highly unsaturated fatty acids, women have higher blood levels of docosahexaenoic acid (DHA; 22:3n-6) as compared with men. Increased conversion of alpha-linolenic acid (ALA; 18:3n-3) to DHA by females has been confirmed in fatty acid stab...


    pub_type: 杂志文章,评审


    authors: Kitson AP,Stroud CK,Stark KD

    更新日期:2010-03-01 00:00:00

  • Suppression of cholesterogenesis by plant constituents: review of Wisconsin contributions to NC-167.

    abstract::In animals, non-sterol metabolites of the mevalonate pathway act independently from receptor-mediated cholesterol uptake in the multivalent feedback regulation of mevalonate biosynthesis. Studies leading to the isolation and characterization of plant-borne suppressors of mevalonate biosynthesis are reviewed. We propos...


    pub_type: 杂志文章


    authors: Qureshi AA,Burger WC,Peterson DM,Elson C

    更新日期:1985-11-01 00:00:00

  • Docosahexaenoic Acid Incorporation Is Not Affected by Doxorubicin Chemotherapy in either Whole Cell or Lipid Raft Phospholipids of Breast Cancer Cells in vitro and Tumor Phospholipids in vivo.

    abstract::To better understand how docosahexaenoic acid (DHA) improves the effects of doxorubicin (DOX), we examined DHA ± DOX on changes in whole cell and lipid raft phospholipids (PL) of MDA-MB-231 and MCF-7 breast cancer cells. We sought to confirm whether the relative changes in PL DHA content of MDA-MB-231 cells could be e...


    pub_type: 杂志文章


    authors: Newell M,Patel D,Goruk S,Field CJ

    更新日期:2020-09-01 00:00:00

  • Sex- and age-related differences in ceramide dihexosides of primary human brain tumors.

    abstract::Neutral glycolipids (NGL) are promising diagnostic markers of human gliomas, but differences in NGL with age and sex have not been examined. Previous work demonstrated that ceramide dihexosides (CDH) levels in mouse kidney are age- and sex-dependent, probably due to levels of sex hormones. We quantitated CDH in 181 hu...


    pub_type: 杂志文章


    authors: Yates AJ,Franklin TK,Scheithauer BW,Burger PC,Pearl DK

    更新日期:1999-01-01 00:00:00

  • Gamma linolenic acid attenuates cardiovascular responses to stress in borderline hypertensive rats.

    abstract::The purpose of the present study was to investigate the effects of gamma linolenic acid (GLA) on cardiovascular responses to psychosocial stress (isolation) and to pressor hormones in the genetically borderline hypertensive rat (SHR X WKY). Adult male SHR X WKY were divided into two groups following five weeks of grou...


    pub_type: 杂志文章


    authors: Mills DE,Summers MR,Ward RP

    更新日期:1985-09-01 00:00:00

  • pH gradient electrophoresis and isoelectric focusing of lipoproteins on agarose bead thin layers.

    abstract::A new method of isoelectric focusing (IEF) and pH gradient electrophoresis, using thin layers of agarose gel beads, was devised to investigate chylomicrons and very low density lipoproteins (VLDL). pH gradient stability and cathodal gradient drift were similar to those of polyacrylamide gel IEF, and linearity of gradi...


    pub_type: 杂志文章


    authors: Vost A,Pocock DM,Pleet S

    更新日期:1979-10-01 00:00:00

  • Olive oil consumption and reduced incidence of hypertension: the SUN study.

    abstract::Olive oil, a major component of the Mediterranean diet, has been associated in some small clinical trials and cross-sectional studies with a reduction in blood pressure. The objective of this study was to assess the association of olive oil consumption with the incidence of hypertension in an epidemiologic cohort, the...


    pub_type: 临床试验,杂志文章


    authors: Alonso A,Martínez-González MA

    更新日期:2004-12-01 00:00:00

  • Formation of cleavage products by autoxidation of lycopene.

    abstract::The cleavage products formed by autoxidation of lycopene were evaluated in order to elucidate possible oxidation products of lycopene in biological tissues. Lycopene solubilized at 50 microM in toluene, aqueous Tween 40, or liposomal suspension was oxidized by incubating at 37 degrees C for 72 h. Among a number of oxi...


    pub_type: 杂志文章


    authors: Kim SJ,Nara E,Kobayashi H,Terao J,Nagao A

    更新日期:2001-02-01 00:00:00

  • Changes in lipid content, fatty acid composition and lipoprotein lipase activity in dry goat omental adipose tissue according to tissue site.

    abstract::Water and lipid contents, fatty acid distribution, and lipoprotein lipase activity were determined in 11 samples taken from the Omentum Majus of five dry Alpine goats. Samples were chosen to standardize sampling sites using geometric guide marks representative of different adipose tissue sites. Sample location explain...


    pub_type: 杂志文章


    authors: Bas P,Gallouin F,Morand-Fehr P

    更新日期:1992-06-01 00:00:00

  • Down-regulation of hepatic stearoyl-CoA desaturase-1 expression by fucoxanthin via leptin signaling in diabetic/obese KK-A(y) mice.

    abstract::Stearoyl-coenzyme A desaturase-1 (SCD1) is a rate-limiting enzyme that catalyzes the biosynthesis of monounsaturated fatty acids from saturated fatty acids. Recently, SCD1 down-regulation has been implicated in the prevention of obesity, and the improvement of insulin and leptin sensitivity. In this study, we examined...


    pub_type: 杂志文章


    authors: Beppu F,Hosokawa M,Yim MJ,Shinoda T,Miyashita K

    更新日期:2013-05-01 00:00:00

  • Solubilization and partial purification of cholinephosphotransferase in hamster tissues.

    abstract::CDPcholine:1,2-diacylglycerol cholinephosphotransferase (EC is located on the cytoplasmic side of the endoplasmic reticulum, and catalyzes the final step in the synthesis of phosphatidylcholine via the CDPcholine pathway. The enzyme was solubilized from hamster liver microsomes by 3% Triton QS-15, and partial...


    pub_type: 杂志文章


    authors: O KM,Choy PC

    更新日期:1990-02-01 00:00:00

  • A maternal high fat diet has long-lasting effects on skeletal muscle lipid and PLIN protein content in rat offspring at young adulthood.

    abstract::A maternal high fat diet (HFD) can have adverse effects on skeletal muscle development. Skeletal muscle PLIN proteins (PLIN2, 3 and 5) are thought to play critical roles in lipid metabolism, however effects of HFD on PLIN and lipases (HSL, ATGL, CGI-58) in mothers as well as their offspring have yet to be investigated...


    pub_type: 杂志文章


    authors: MacPherson RE,Castelli LM,Miotto PM,Frendo-Cumbo S,Milburn A,Roy BD,LeBlanc PJ,Ward WE,Peters SJ

    更新日期:2015-02-01 00:00:00

  • Effects of dietary corn oil and salmon oil on lipids and prostaglandin E2 in rat gastric mucosa.

    abstract::Three groups of male rats were fed either a corn oil-enriched diet (17%, w/w), a salmon oil-enriched diet (12.5%) supplemented with corn oil (4.5%) or a low-fat diet (4.4%) for eight wk to investigate the possible relationships between dietary fatty acids and lipid composition, and prostaglandin E2 level and phospholi...


    pub_type: 杂志文章


    authors: Grataroli R,Léonardi J,Charbonnier M,Lafont R,Lafont H,Nalbone G

    更新日期:1988-07-01 00:00:00

  • Effect of dietary linoleic acid content on the distribution of triacylglycerol molecular species in rat adipose tissue.

    abstract::The present study examined the effect of varying dietary linoleate intake (0.01, 0.24, 2.4, 24, 80 or 160 g/kg diet) for 24 weeks on the distribution of triacylglycerol (TG) molecular species in rat epididymal adipose tissue. Adipose TG fractions were purified by thin-layer chromatography and separated into different ...


    pub_type: 杂志文章


    authors: Huang YS,Lin X,Smith RS,Redden PR,Jenkins DK,Horrobin DF

    更新日期:1992-09-01 00:00:00

  • A new quantitative method for the analysis of monoacylglycerol isomers using(13)C nuclear magnetic resonance spectroscopy.

    abstract::Current methods for determining the regiochemistry of monoacylglycerols are lengthy, tedious and aggravated by the ready isomerization of 2-acyl-sn-glycerols. A new method employs a very rapid adsorption chromatography step in which isomerization is kept to a minimum, followed by formation of the isopropylidene deriva...


    pub_type: 杂志文章


    authors: Dawe RD,Wright JJ

    更新日期:1988-04-01 00:00:00

  • Novel long-chain diol phospholipids from some bacteria belonging to the class Thermomicrobia.

    abstract::Polar lipids of bacteria from the class Thermomicrobia are known to contain long-chain 1,2-diols instead of glycerol, although the nature of polar head groups has not been investigated. We have studied phospholipid classes of two species from the class Thermomicrobia-Sphaerobacter thermophilus and Thermomicrobia sp. W...


    pub_type: 杂志文章


    authors: Lagutin K,MacKenzie A,Houghton KM,Stott MB,Vyssotski M

    更新日期:2015-03-01 00:00:00

  • Polyphosphoinositide formation in isolated cardiac plasma membranes.

    abstract::Phosphatidylinositol (PtdIns) and phosphatidylinositol 4-phosphate (PtdIns4P) kinase activities in plasma membranes isolated from canine left ventricle were partially characterized, and their sensitivity to a number of intracellular variables was established. PtdIns and PtdIns4P kinase activities were estimated by the...


    pub_type: 杂志文章


    authors: Kasinathan C,Xu ZC,Kirchberger MA

    更新日期:1989-09-01 00:00:00

  • Lysophosphatidic acid produced by hen egg white lysophospholipase D induces vascular development on extraembryonic membranes.

    abstract::Lysophosphatidic acid (lysoPtdOH), a lysophospholipid mediator, exerts diverse physiological effects, including angiogenesis, through its specific G-protein-coupled receptors. Previously, we showed that unfertilized hen egg white contains polyunsaturated fatty acid-rich lysoPtdOH and lysophospholipase D (lysoPLD). Her...


    pub_type: 杂志文章


    authors: Morishige J,Uto Y,Hori H,Satouchi K,Yoshiomoto T,Tokumura A

    更新日期:2013-03-01 00:00:00

  • Effect of the selective PAF antagonist SM-10661 on an asthmatic model. 1. Effect on passive anaphylactic bronchoconstriction in guinea pigs.

    abstract::The effect of SM-10661, a selective antagonist of platelet-activating factor (PAF), on passive anaphylactic bronchoconstriction was examined in guinea pigs. A challenge of ovalbumin to passively sensitized guinea pigs induced bronchoconstriction, which peaked at 4 min. When SM-10661 was administered intravenously 2 mi...


    pub_type: 杂志文章


    authors: Uchida M,Imanishi N,Sugasawa T,Morooka S

    更新日期:1991-12-01 00:00:00

  • Substrate preferences of a lysophosphatidylcholine acyltransferase highlight its role in phospholipid remodeling.

    abstract::An important enzyme involved in phospholipid turnover is the acyl-CoA: lysophosphatidylcholine acyltransferase (LPCAT). Here, we report characterization of a newly discovered human LPCAT (LPCAT3), which has distinct substrate preferences strikingly consistent with a role in phosphatidylcholine (PtdCho) remodeling and ...


    pub_type: 杂志文章


    authors: Kazachkov M,Chen Q,Wang L,Zou J

    更新日期:2008-10-01 00:00:00

  • Derivatization of keto fatty acids, X: Synthesis of thiazanones.

    abstract::The synthesis of alkyl chain-substituted thiazanones from oxo fatty esters and a long chain aldehyde is reported. Four oxo compounds-methyl 10-oxoundecanoate, methyl 9-oxooctadecanoate, methyl 9,10-dioxooctadecanoate and octadecanal-were allowed to react with β-mercaptopropionic acid in the presence of ammonium carbon...


    pub_type: 杂志文章


    authors: Khan A,Husain SR,Ahmad F,Siddiqui MS,Pimlott W

    更新日期:1987-08-01 00:00:00

  • Hepatic bile acid elution by albumin and bile acid content in isolated rat hepatocytes.

    abstract::Bile acid contents were determined for isolated rat hepatocytes. During the course of isolating the hepatocytes, perfusion of rat liver with buffer containing 2% albumin eluted a significant amount of bile acids. The elution was proportional to the volume of the buffer and attributable to albumin in the buffer. The is...


    pub_type: 杂志文章


    authors: Hashimoto S,Uchida K,Hirata M

    更新日期:1982-03-01 00:00:00

  • Apparent lack of effect of obesity on the soluble phosphatidic acid phosphatase activity in human adipose tissue.

    abstract::In view of previous reports that the activity of the Mg(++)-dependent phosphatidic acid phosphatase in adipose tissues of rat and mouse is elevated in obesity, we attempted to assay this activity in biopsies of human omental adipose tissue obtained from normal-weight and morbidly obese subjects in connection with oper...


    pub_type: 杂志文章


    authors: Björkhem I,al-Shurbaji A,Backman L,Arner P

    更新日期:1989-12-01 00:00:00