Abstract:
:A method is described for the determination of the positions and geometric configurations of double bonds in polyunsaturated fatty acids. The procedure consists of three steps: 1) Partial reduction of the double bonds with hydrazine under conditions which give high yields of monoenes. 2) Isolation of thecis- and thetrans-monoene fractions by thin-layer chromatography (TLC) directly or in the form of their ozonide derivatives. In the former technique, selective argentation is employed, in the latter, silicic acid adsorption. 3) Determination of the structure of the monoenes via reductive ozonolysis. The position of the double bonds is determined from the structures of the monoenes. Since thecis-monoenes are separated from thetrans-monoenes the geometric configuration of each double bond is determined.The method also provides a direct determination of the spacings of the internal double bonds and it may be employed for the determination of the structures of mixtures of fatty acids in conjunction with direct ozonolysis procedures. The various ramifications of the method are demonstrated on pure fatty acids and model mixtures thereof.
journal_name
Lipidsjournal_title
Lipidsauthors
Privett OS,Nickell ECdoi
10.1007/BF02532998subject
Has Abstractpub_date
1966-03-01 00:00:00pages
98-103issue
2eissn
0024-4201issn
1558-9307journal_volume
1pub_type
杂志文章相关文献
LIPIDS文献大全abstract::The use of the organic cosolvents tetrahydrofuran and dimethylsulfoxide was found to be unsuitable for prostate tumor cell cultures because of solvent cytotoxicity and the poor solubility and instability of lycopene. For example, the half-life of lycopene in organic/aqueous solution was found to be less than 2 h. Ther...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-999-0454-9
更新日期:1999-10-01 00:00:00
abstract::The gut microbiota is proposed as a "metabolic organ" involved in energy utilization and is associated with obesity. Dietary intervention is one of the approaches for obesity management. Changes in dietary components have significant impacts on host metabolism and gut microbiota. In the present study, we examined the ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-017-4253-2
更新日期:2017-06-01 00:00:00
abstract::The aim of this study was to determine the influence of long-term docosahexaenoic acid (DHA) dietary supplementation on the erythrocyte fatty acid profile and oxidative balance in soccer players after training and acute exercise. Fifteen volunteer male athletes (age 20.0 ± 0.5 years) were randomly assigned to a placeb...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3976-6
更新日期:2015-02-01 00:00:00
abstract::Starting at 21 days of age, groups of six rats each were fed a basal Torula yeast diet supplemented with 0.4% L-methionine and varying amounts of vitamin E as dl-alpha tocopherol acetate, selenium as sodium selenite, and with either 10% stripped corn oil, stripped lard, or coconut oil. By 7 wk, pentane production by r...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533708
更新日期:1978-06-01 00:00:00
abstract::The source of free fatty acids (FFA) and the pathways contributing to the accumulation of neutral fats in livers of rats fed a cholesterol-enriched diet were investigated in this report. Supplementation with 1% cholesterol in the diet for four weeks resulted in hepatomegaly in the rats. The contents of cholesterol and...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537027
更新日期:1995-06-01 00:00:00
abstract::Neutrophils play a major role in inflammation by releasing large amounts of reactive oxygen species (ROS) produced by NADPH oxidase (NOX) and myeloperoxidase (MPO). This ROS overproduction is mediated by phosphorylation of the NOX subunits in an uncontrolled manner. Therefore, targeting neutrophil subunits would repre...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-013-3863-6
更新日期:2014-01-01 00:00:00
abstract::The levels of arachidonic acid and linoleic acid found in the lipid fraction of human milk samples were compared. No correlation was found between the level of precursor linoleate and product arachidonate in 80 samples of colostrum (day 3-5) or 60 samples of mature breast milk. We attempted to test the hypothesis that...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537411
更新日期:1984-06-01 00:00:00
abstract::The impact of starch sources differing in their velocities of ruminal degradation on the milk fat of dairy cows was studied. The animals received diets containing a slowly degradable (potatoes) or rapidly degradable (wheat) starch concentrate (40% of the dry matter) in a total mixed diet. Milk fat was the only animal ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-004-1196-4
更新日期:2004-01-01 00:00:00
abstract::Exercise training not only improves the plasma lipid profile but also reduces risk of developing coronary heart disease. We investigate whether plasma lipids and high density lipoprotein (HDL) metabolism are affected by aerobic training and whether the high-density lipoprotein cholesterol (HDL-C) levels at baseline in...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12155
更新日期:2019-06-01 00:00:00
abstract::Hydrolysis of synthetic triglycerides by rattlesnake and leopard shark pancreatic enzymes revealed striking differences in specificity, depending on the presence or absence of sodium taurocholate. Without added sodium taurocholate the classical specificity of pancreatic lipase was expressed. Rattlesnake enzymes, in th...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532361
更新日期:1975-09-01 00:00:00
abstract::There have been a limited number of studies investigating surfactant lipid changes in lung with trace elements. The present investigation was designed to examine the effect of moderate zinc deficiency on the lipid metabolism in rat lung. We also evaluated whether zinc deficiency, which is a wide-spread problem, could ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0893-3
更新日期:2002-03-01 00:00:00
abstract::The effects of 2-hexadecynoic acid on the growth and lipid metabolism of cultured 7288 (HTC) cells have been evaluated. Growth was inhibited by the acetylenic acid: the LD50 was 35-85 microM as determined by two methods at low and high cell densities. Reduced growth did not result from damaged plasma membranes as dete...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534955
更新日期:1981-05-01 00:00:00
abstract::Mevalonic acid-derived intermediates in the cholesterol biosynthetic pathway have been recognized as being critical to the isoprenylation of a variety of growth-regulating proteins, including those of the RAS superfamily. Treatment of cells with lovastatin, a hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase inhibi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0879-1
更新日期:2002-02-01 00:00:00
abstract::Fatty acid composition of plasma phospholipids (PL), triglycerides (TG), and sterol esters (STE) was determined by high-resolution capillary gas-liquid chromatography in 22 obese children (age: 13.7 +/- 1.4 y, body weight relative to normal weight for height: 170 +/- 24%, mean +/- SD) and compared with data obtained i...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02529877
更新日期:1996-03-01 00:00:00
abstract::Chloroquine is shown to be a potent inhibitor of cholesterol biosynthesis by isolated rat hepatocytes. Half-maximal inhibition of cholesterogenesis occurs at ca. 10 micro M chloroquine. Chloroquine does not affect fatty acid synthesis by isolated hepatocytes. This suggests that chloroquine acts on the cholesterol bios...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535017
更新日期:1981-06-01 00:00:00
abstract::The weight loss supplement conjugated linoleic acid (CLA) consists of an equal mixture of trans-10,cis-12 (10,12) and cis-9,trans-11 (9,11) isomers. However, high levels of mixed CLA isomers, or the 10,12 isomer, causes chronic inflammation, lipodystrophy, or insulin resistance. We previously demonstrated that 10,12 C...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-012-3711-0
更新日期:2012-11-01 00:00:00
abstract::Electron paramagnetic resonance (EPR) in conjunction with a slow-tumbling simulation was utilized for defining stratum corneum (SC) lipid structure. SC from the back of hairless mouse (HOS:HR-1) was stripped consecutively from one to three or four times using a glass plate coated with a cyanoacrylate resin. Aliphatic ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-010-3479-z
更新日期:2010-11-01 00:00:00
abstract::Total lipids from whole pig adrenal glands as well as from their mitochondria, microsomes, liposomes, and cell sap were extracted and fractionated first into neutral lipids and phospholipids. The highest percentage of neutral lipids was found in the cell sap, and the lowest in the microsomal fraction. Neutral lipids w...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532765
更新日期:1975-11-01 00:00:00
abstract::We studied the actions of oxysterols on fatty acid distribution and lipid synthesis in cultured bovine aortic smooth muscle cells. Cultures were labeled with [1-14C]arachidonate or [1-14C]oleate. During a 24-hr incubation, 25- or 22R-hydroxycholesterol enhanced the incorporation of label into triglycerides, concomitan...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544334
更新日期:1990-03-01 00:00:00
abstract::Two electrophoretically different forms of sterol ester hydrolase (EC 3.1.1.13) were obtained from the cytoplasmic extract of the mycelia of Fusarium oxysporum. The entities, estimated at 60,000 (I) and 15,000 (II) molecular weights, were obtained in Sephadex G100 column chromatography of the ammonium sulfate precipit...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535686
更新日期:1981-02-01 00:00:00
abstract::Paraoxonase 1 (PON 1) has antioxidant and cardioprotective properties and is abnormally low in type 2 diabetic serum. This study aimed to determine the effect of type 2 diabetes and meals rich in saturated fat and oleic acid on PON1 activity in chylomicrons and very low density lipoproteins (VLDL). PON1 arylesterase a...
journal_title:Lipids
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s11745-011-3640-3
更新日期:2012-03-01 00:00:00
abstract::Differences in the positional incorporation of 9-trans[1-(14)C] octadecenoic (elaidic) and 9-trans,12-trans[1-(14)C] octadecadienoic (linoelaidic) acids in fetal lecithin of rats were demonstrated. On the 20th day of gestation, a 14C-labeled albumin complex of elaidic or linoelaidic acid was injected into the jugular ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535045
更新日期:1981-07-01 00:00:00
abstract::The production of cardiac arrhythmias and the elevation of lysophosphatidylcholine level in the ischemic myocardium have been well-documented in a number of studies. However, the relationship between the production arrhythmias and the elevation of tissue lysophosphatidylcholine level was not reported. In this study, t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535301
更新日期:1988-01-01 00:00:00
abstract::The effect of various physicochemical forms of substrate on the activity of acid cholesteryl ester hydrolase isolated from rat liver lysosomes was studied. The amount of sodium taurocholate was varied in the substrate mixture which contained constant amounts of egg phosphatidylcholine (PC) and cholesteryl oleate. The ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535632
更新日期:1986-08-01 00:00:00
abstract::To test the effects of eicosapentaenoic acid (EPA) infusion on pulmonary edema induced by coronary ligation and reperfusion, extravascular lung water (EVLW) was measured in situ by the thermal-dye double indicator dilution method in dogs. In the control group of five dogs, 30 mL of a 10% soybean oil emulsion was infus...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-997-0015-2
更新日期:1997-01-01 00:00:00
abstract::FA ethyl esters (FAEE) are nonoxidative metabolites of ethanol produced by the esterification of FA and ethanol. FAEE have been implicated as mediators of ethanol-induced organ damage in vivo and in vitro, and are markers of ethanol intake. Upon ethanol intake, FAEE are synthesized in the liver and pancreas in signifi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-1160-3
更新日期:2003-10-01 00:00:00
abstract::Fourteen pairs of obese female monozygotic twins were recruited for a study of genetic influences on serum and adipose fatty acid (FA) composition. Following 1 wk of inpatient stabilization, fasting serum and adipose tissue obtained by surgical excision were analyzed by thin-layer and gas chromatography. Intrapair res...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0860-z
更新日期:2002-01-01 00:00:00
abstract::The effect of simvastatin, a hypocholesterolemic drug, on the biosynthesis of arachidonic acid was studied in obese and lean Zucker rats. After administration of 2 mg/kg body weight/d for 13 d, delta 6 and delta 5 desaturase activities were measured in liver microsomes at two substrate concentrations. In untreated rat...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536363
更新日期:1993-01-01 00:00:00
abstract::In offspring exposed in utero to a maternal diet high in fat (HF), we have previously demonstrated that despite similar birth weights, HF adult offspring at 6 months of age had significantly higher body weights, greater adiposity, and increased triacylglycerol (TAG) levels as compared to controls. We hypothesized that...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-015-4018-8
更新日期:2015-06-01 00:00:00
abstract::The effects of different dietary fats on ovine adipose tissue metabolism have been investigated. Six-month old sheep were fed for 6 weeks a control diet or diets supplemented with either tallow or a mixture of sunflower seed oil and soybean oil, treated to protect the fats from hydrolysis and hydrogenation in the rume...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532883
更新日期:1976-09-01 00:00:00