Krill Oil Perturbs Proliferation and Migration of Mouse Colon Cancer Cells in vitro by Impeding Extracellular Signal-Regulated Protein Kinase Signaling Pathway.

Abstract:

:The prevalence of colorectal cancer (CRC) continues to increase. Treatment of CRC remains a significant clinical challenge, and effective therapies for advanced CRC are desperately needed. Increasing attention and ongoing research efforts have focused on krill oil that may provide health benefits to the human body. Here we report that krill oil exerts in vitro anticancer activity through a direct inhibition on proliferation, colony formation, migration, and invasion of mouse colon cancer cells. Krill oil inhibited the proliferation and colony formation of CT-26 colon cancer cells by causing G0/G1 cell cycle arrest and apoptosis. Cell cycle arrest was attributable to reduction of cyclin D1 levels in krill oil-treated cells. Further studies revealed that krill oil induced mitochondrial-dependent apoptosis of CT-26 cells, including loss of mitochondrial membrane potential, increased cytosolic calcium levels, activation of caspase-3, and downregulation of anti-apoptotic proteins MCL-1 and BCL-XL. Krill oil suppressed migration of CT-26 cells by disrupting the microfilaments and microtubules. Extracellular signal-regulated protein kinase (ERK) plays crucial roles in regulating proliferation and migration of cancer cells. We found that krill oil attenuated the activation of ERK signaling pathway to exert the effects on cell cycle, apoptosis, and migration of colon cancer cells. We speculate that polyunsaturated fatty acids of krill oil may dampen ERK activation by decreasing the phospholipid saturation of cell membrane. Although findings from in vitro studies may not necessarily translate in vivo, our study provides insights into the possibility that krill oil or its components could have therapeutic potential in colon cancer.

journal_name

Lipids

journal_title

Lipids

authors

Jing W,Bi Y,Wang G,Zeng S,Han L,Yang H,Wang N,Zhao Y

doi

10.1002/lipd.12281

subject

Has Abstract

pub_date

2020-09-15 00:00:00

eissn

0024-4201

issn

1558-9307

pub_type

杂志文章

相关文献

LIPIDS文献大全
  • Contrasting effects of t10,c12- and c9,t11-conjugated linoleic acid isomers on the fatty acid profiles of mouse liver lipids.

    abstract::The purpose of this study was to examine the effects of two purified isomers of CLA (c9,t11-CLA and t10,c12-CLA) on the weights and FA compositions of hepatic TG, phospholipids, cholesterol esters, and FFA. Eight-week-old female mice (n = 6/group) were fed either a control diet or diets supplemented with 0.5% c9,t11-C...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-004-1211-9

    authors: Kelley DS,Bartolini GL,Warren JM,Simon VA,Mackey BE,Erickson KL

    更新日期:2004-02-01 00:00:00

  • Effects of high levels of dietary fats on the growth of azaserine-induced foci in the rat pancreas.

    abstract::Azaserine induced two phenotypically different populations of foci, namely, acidophilic and basophilic foci. The effects of dietary modification during the post-initiation phase of carcinogenesis were examined. A diet of 20% (w/w) unsaturated fat (unsat) compared to a 20% saturated fat (sat) diet or a control diet (5%...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02536413

    authors: Roebuck BD

    更新日期:1986-04-01 00:00:00

  • Successful utilization of lyophilized lipoprotein(a) as a biological reagent.

    abstract::Lipoprotein(a) [Lp(a)] represents a class of lipoprotein particles having as a protein moiety apoB-100 linked by a single disulfide bond to apolipoprotein(a) [apo(a)], a multikringle structure with a high degree of homology with plasminogen. A recognized feature of Lp(a) is its instability on storage caused by attenda...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-004-1268-5

    authors: Scanu AM,Hinman J,Pfaffinger D,Edelstein C

    更新日期:2004-06-01 00:00:00

  • Effects of ethionine and phenobarbital on the phosphatidylcholines of rat liver.

    abstract::The diglyceride species of phosphatidylcholines from livers of male rats after treatment of the animals with ethionine (1 mg/g divided among 4 doses 2 hr apart), phenobarbital (80 mg/kg each day for 3 days), or a combination of the two drugs were determined using gas chromatography. Ethionine treatment greatly elevate...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533307

    authors: Dyer RA,Klopfenstein WE

    更新日期:1977-11-01 00:00:00

  • Docosahexaenoic Acid Incorporation Is Not Affected by Doxorubicin Chemotherapy in either Whole Cell or Lipid Raft Phospholipids of Breast Cancer Cells in vitro and Tumor Phospholipids in vivo.

    abstract::To better understand how docosahexaenoic acid (DHA) improves the effects of doxorubicin (DOX), we examined DHA ± DOX on changes in whole cell and lipid raft phospholipids (PL) of MDA-MB-231 and MCF-7 breast cancer cells. We sought to confirm whether the relative changes in PL DHA content of MDA-MB-231 cells could be e...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1002/lipd.12252

    authors: Newell M,Patel D,Goruk S,Field CJ

    更新日期:2020-09-01 00:00:00

  • Biosynthesis and tissue deposition of docosahexaenoic acid (22:6n-3) in rainbow trout (Oncorhynchus mykiss).

    abstract::Rainbow trout (Oncorhynchus mykiss) weighing ca. 5 g and previously acclimated for 8 wk on a diet comprising vegetable oil (11%), fish meal (5%), and casein (48%) as the major constituents were fed a pulse of diet containing deuterated (D5) (17,17,18,18,18)-18:3n-3 ethyl ester. The synthesis and tissue distribution of...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-001-0826-1

    authors: Bell MV,Dick JR,Porter AE

    更新日期:2001-10-01 00:00:00

  • Quantification, characterization and fatty acid composition of lysophosphatidic acid in different rat tissues.

    abstract::The amount and composition of lysophosphatidate present in different rat tissues have been estimated by an internal standard method in which a synthetic unnatural isomer (1-heptadecanoyl-rac-glycerol-3-phosphate) was added to the total lipid extracts, and the fatty acid composition of purified lysophosphatidate was de...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535172

    authors: Das AK,Hajra AK

    更新日期:1989-04-01 00:00:00

  • CETP and LCAT Gene Polymorphisms Are Associated with High-Density Lipoprotein Subclasses and Acute Coronary Syndrome.

    abstract::We evaluated whether CETP and LCAT gene polymorphisms are statistically associated with the high-density lipoprotein (HDL) size distribution, the cholesterol level of HDL subclasses, and the acute coronary syndrome (ACS) susceptibility. Two CETP gene polymorphisms (rs4783961 and rs708272) and one LCAT polymorphism (rs...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1002/lipd.12017

    authors: Vargas-Alarcon G,Perez-Mendez O,Herrera-Maya G,Garcia-Sanchez C,Martinez-Rios MA,Peña-Duque MA,Posadas-Sanchez R,Posadas-Romero C,Escobedo G,Fragoso JM

    更新日期:2018-02-01 00:00:00

  • Novel oxylipins formed from docosahexaenoic acid by potato lipoxygenase--10(S)-hydroxydocosahexaenoic acid and 10,20-dihydroxydocosahexaenoic acid.

    abstract::Potato tuber lipoxygenase (ptLOX) has been shown to catalyze the aerobic formation of at least four major oxygenated derivatives of DHA. Two of the products--7,17(S)- and 10,17(S)-dihydro(pero)xy-DHA [7,17- and 10,17-diH(P)DHA]--were formed from soybean 15-LOX-derived 17(S)-hydro(pero)xy-DHA [17(S)-H(P)DHA], whereas t...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-005-1379-z

    authors: Butovich IA,Hamberg M,Rådmark O

    更新日期:2005-03-01 00:00:00

  • Rapid and improved method for the determination of bile acids in human feces using MS.

    abstract::A simple method for the determination of bile acids in adult human fecal samples using GC-MS is described. Bile acids are directly extracted from feces by ethanol (95%) containing 0.1 N NaOH. Extracts are purified by passage through a reversed-phase C18 silica cartridge and then analyzed by GC-MS. The present study ha...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-002-1005-0

    authors: Perwaiz S,Mignault D,Tuchweber B,Yousef IM

    更新日期:2002-11-01 00:00:00

  • Dietary phospholipids are more efficient than neutral lipids for long-chain polyunsaturated fatty acid supply in European sea bass Dicentrarchus labrax larval development.

    abstract::We evaluated the effects of dietary lipid class (phospholipid vs. neutral lipid) and level of n-3 long-chain PUFA (LC-PUFA) on the growth, digestive enzymatic activity, and histological organization of the intestine and liver in European sea bass larvae. Fish were fed from the onset of exogenous feeding at 7 to 37 d p...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-005-1422-0

    authors: Gisbert E,Villeneuve L,Zambonino-Infante JL,Quazuguel P,Cahu CL

    更新日期:2005-06-01 00:00:00

  • The effects of dehydroepiandrosterone on carcinogenesis, obesity, the immune system, and aging.

    abstract::With the passage of the U.S. Dietary Supplement Health and Education Act of 1994, dehydroepiandrosterone (DHEA, 5-androsten-3beta-ol-17-one) has become widely available, and a large and growing market has developed for this "fountain of youth." DHEA has been shown to have significant beneficial effects in animals, whi...

    journal_title:Lipids

    pub_type: 杂志文章,评审

    doi:10.1007/s11745-000-0529-7

    authors: Williams JR

    更新日期:2000-03-01 00:00:00

  • Effects of growth temperature on the fatty acid composition of the free-living nematode Caenorhabditis elegans.

    abstract::The effects of growth temperature on the fatty acid compositions of the phosphatidylcholine (PC), phosphatidylethanolamine (PE), and total lipid (TL) fractions of the free-living nematode Caenorhabditis elegans were investigated. A reduction in growth temperature from 25 to 15 degrees C caused the proportions of eicos...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02524292

    authors: Tanaka T,Ikita K,Ashida T,Motoyama Y,Yamaguchi Y,Satouchi K

    更新日期:1996-11-01 00:00:00

  • Inevitable generation of primary alcohols during reduction of oxidized lipids with sodium borohydride.

    abstract::This report deals with the fluorometric determination of fatty alcohols generated by the reduction of the ester linkage of lipids with NaBH4, and with the limitations of the reduction method for assaying oxidized lipids. Optimum conditions for the fluorometric analysis of primary and secondary alcohols using 1-anthroy...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02537169

    authors: Nakamura T,Maeda H,Takahashi Y,Hama Y

    更新日期:1990-09-01 00:00:00

  • The differential effect of eicosapentaenoic acid and oleic acid on lipid synthesis and VLDL secretion in rabbit hepatocytes.

    abstract::The suppression of plasma very low density lipoprotein (VLDL) triglyceride levels by dietary fish oils rich in polyunsaturated n-3 fatty acids has been attributed to decreased hepatic VLDL secretion. To investigate the effect of n-3 fatty acids on lipid metabolism and VLDL secretion in a tissue culture system, we incu...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02537160

    authors: Benner KG,Sasaki A,Gowen DR,Weaver A,Connor WE

    更新日期:1990-09-01 00:00:00

  • Preparation and properties of various salt forms of plant phosphatidyl inositols.

    abstract::The Ca and Mg content of flax and corn phosphatidyl inositol fractions has been determined. Procedures were devised to prepare various salt forms of phosphatidyl inositol. The divalent cations were exchanged for monovalent ions (Na or K) on chelating resin columns. With the Folch wash procedure the Na or K forms of ph...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02668119

    authors: Carter HE,Weber EJ

    更新日期:1966-01-01 00:00:00

  • Relationship between mouse liver delta 9 desaturase activity and plasma lipids.

    abstract::This study was undertaken to investigate the total plasma fatty acid composition and the relationship between plasma triacylglycerol (TG) levels and liver delta 9 desaturase activity in mice fed n-3 and/or n-6 fatty acid or hydrogenated coconut oil (HCO) (maximum 25 mg/g) supplemented diets. Generally, plasma TG level...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02536312

    authors: de Antueno RJ,Cantrill RC,Huang YS,Elliot M,Horrobin DF

    更新日期:1993-04-01 00:00:00

  • Two new icosapentaenoic acids from the temperate red seaweed Ptilota filicina J. Agardh.

    abstract::Two new fatty acid metabolites, 5(Z),7(E),9(E),14(Z),17(Z)-icosapentaenoic acid and 5(E),7(E),9(E),14(Z),17(Z)-icosapentaenoic acid, have been isolated from the temperate red marine alga, Ptilota filicina (Ceramiales, Rhodophyta). The structures of these new compounds, isolated as their methyl ester derivatives, have ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02537301

    authors: Lopez A,Gerwick WH

    更新日期:1987-03-01 00:00:00

  • Apolipoprotein E polymorphism: automated determination of apolipoprotein E2, E3, and E4 isoforms.

    abstract::Apolipoprotein E (apo E) plays an essential role in lipoprotein metabolism, where it is involved in the clearance of chylomicrons and very low density lipoproteins. Apart from some rare variants, apo E exists in three common isoforms (E2, E3, and E4). The different isoforms have not only been associated with different...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-000-0500-7

    authors: Wiebe C,Holzem G,Wielckens K,Klingler KR

    更新日期:2000-01-01 00:00:00

  • Cyclic fatty acid monomers from dietary heated fats affect rat liver enzyme activity.

    abstract::This study was conducted to investigate the effects of dietary cyclic fatty acid monomers (CFAM), contained in heated fat from a commercial deep-fat frying operation, on rat liver enzyme activity. A partially hydrogenated soybean oil (PHSBO) used 7 d (7-DH) for frying foodstuffs, or 0.15% methylated CFAM diets was fed...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-998-0256-0

    authors: Lamboni C,Sébédio JL,Perkins EG

    更新日期:1998-07-01 00:00:00

  • High-Fat Diets Containing Different Amounts of n3 and n6 Polyunsaturated Fatty Acids Modulate Inflammatory Cytokine Production in Mice.

    abstract::Dysregulation of adipokines is a hallmark of obesity. Polyunsaturated fatty acids in fish oil may exert anti-inflammatory effects on adipose tissue mitigating the dysregulation of adipokines thereby preventing obesity. This study investigated the effects of high-fat diets containing different amounts of n3 polyunsatur...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-015-4093-x

    authors: Sundaram S,Bukowski MR,Lie WR,Picklo MJ,Yan L

    更新日期:2016-05-01 00:00:00

  • Lipid content of swine influenza and other vaccines.

    abstract::An analysis of the lipids in swine influenza vaccines was performed, comparing six different lots of swine influenza, other influenza and noninfluenza vaccines. Cholesterol content and phospholipid content varied greatly, but there were no major differences between the types of vaccines. Appreciable amounts of phospha...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535698

    authors: Leskawa KC,Hogan EL,Dasgupta S,Chien JL,Erwin RE,Brostoff SW

    更新日期:1986-05-01 00:00:00

  • The structure of the glycerides of ergot oils.

    abstract::The oils from sclerotia or from suitable mycelial cultures ofClaviceps purpurea (ergot) contain up to 44% of ricinoleic acid but no free hydroxyl groups. This is due to the presence of, besides normal triglycerides, tetra-acid, penta-acid and hexa-acid triglycerides. These contain respectively one, two and three ricin...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02531871

    authors: Morris LJ,Hall SW

    更新日期:1966-05-01 00:00:00

  • High intake, but not low intake, of CLA impairs weight gain in growing mice.

    abstract::CLA has a range of biological properties, including effects on lipid metabolism and body composition in experimental animals. The prevalent isomer of CLA found in the human diet is 9c,11 t-CLA, and it is predominantly found in products containing fat from ruminant animals. This study investigated the effect of dietary...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-002-0950-y

    authors: Hayman A,MacGibbon A,Pack RJ,Rutherfurd K,Green JH

    更新日期:2002-07-01 00:00:00

  • Effects of different fractions of the barley kernel on the hepatic lipid metabolism of chickens.

    abstract::Various physical fractions of the barley kernel were fed to one-day-old female and male chickens to determine their effect on hepatic beta-hydroxy-beta-methylglutaryl coenzyme A (HMG-CoA) reductase, cholesterol 7 alpha-hydroxylase and the lipogenic enzymes, acetyl-CoA carboxylase (ACX), malic enzyme (ME), citrate-clea...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534592

    authors: Burger WC,Qureshi AA,Prentice N,Elson CE

    更新日期:1982-12-01 00:00:00

  • Changes in plasma LDL and HDL composition in patients undergoing cardiac surgery.

    abstract::Changes of lipoprotein composition have been mainly reported in conditions of sepsis. This study characterized compositional changes in LDL and HDL during the acute phase response following cardiac surgery with cardiopulmonary bypass. Twenty-one patients undergoing cardiac surgery were included in this study. Blood sa...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-007-3114-9

    authors: Hacquebard M,Ducart A,Schmartz D,Malaisse WJ,Carpentier YA

    更新日期:2007-12-01 00:00:00

  • Effect of dietary fats on ovine adipose tissue metabolism.

    abstract::The effects of different dietary fats on ovine adipose tissue metabolism have been investigated. Six-month old sheep were fed for 6 weeks a control diet or diets supplemented with either tallow or a mixture of sunflower seed oil and soybean oil, treated to protect the fats from hydrolysis and hydrogenation in the rume...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02532883

    authors: Vernon RG

    更新日期:1976-09-01 00:00:00

  • Sterol synthesis from biliary squalene in the jejunal mucosa of the rat in vivo.

    abstract::Because bile contains substantial amounts of cholesterol precursors, e.g., squalene and different methyl sterols, the fate of biliary squalene was studied by incubating isolated jejunal loops of the rat in vivo with bile containing 3H-squalene and 14C-cholesterol. After 90 min, no radioactivity was found in plasma lip...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535392

    authors: Strandberg TE

    更新日期:1983-08-01 00:00:00

  • Cheek cell fatty acids as indicators of dietary lipids in humans.

    abstract::Analysis of cheek cell lipids has been suggested as a noninvasive method for monitoring the fatty acid composition of diets in humans. In a pilot study conducted to determine the validity of the method, cheek cell samples were collected from subjects consuming a low fat (20% of calories) diet consisting of fatty acids...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535293

    authors: Sampugna J,Light L,Enig MG,Jones DY,Judd JT,Lanza E

    更新日期:1988-02-01 00:00:00

  • The structure-activity relationship of lipoxygenase products of long-chain polyunsaturated fatty acids: effects on human platelet aggregation.

    abstract::The effect of hydroperoxy and hydroxy derivatives of various fatty acids on human platelet aggregation was determined to delineate potencies and structure-activity function. In this regard, the 22-carbon n-3 fatty acids are the most potent inhibitors in comparison to the n-6 lipoxygenase derivatives. Submicromolar lev...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02637097

    authors: Karanian JW,Kim HY,Salem N Jr

    更新日期:1996-03-01 00:00:00