Successful utilization of lyophilized lipoprotein(a) as a biological reagent.

Abstract:

:Lipoprotein(a) [Lp(a)] represents a class of lipoprotein particles having as a protein moiety apoB-100 linked by a single disulfide bond to apolipoprotein(a) [apo(a)], a multikringle structure with a high degree of homology with plasminogen. A recognized feature of Lp(a) is its instability on storage caused by attendant protein and lipid modifications that affect the structural, functional, and immunological properties of this lipoprotein. Here we present data showing that, under appropriate conditions of cryopreservation, Lp(a) retains the properties of the freshly isolated product, and we provide examples supporting the stability of this cryopreserved product as a primary standard in immunoassay settings and in cell culture systems.

journal_name

Lipids

journal_title

Lipids

authors

Scanu AM,Hinman J,Pfaffinger D,Edelstein C

doi

10.1007/s11745-004-1268-5

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

589-93

issue

6

eissn

0024-4201

issn

1558-9307

journal_volume

39

pub_type

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